20600 008041-3 05/01

For in vitro diagnostic use

Identification system for streptococci

API 20 STREP is a standardized method combining 20 biochemical tests that offer widespread capabilities. It enables group or species
identification of most streptococci encountered in medical bacteriology. The complete list of those organisms that it is possible to
identify with this system is given in the Identification Table at the end of this package insert.

PRINCIPLE COMPOSITION OF MEDIA AND REAGENTS

The API 20 STREP strip consists of 20 microtubes containing Suspension Demineralized water
dehydrated substrates for the demonstration of enzymatic activity Medium
2 ml
or the fermentation of sugars. GP Medium Cystine 0.5 g
The enzymatic tests are inoculated with a dense suspension of 2 ml Tryptone 20 g
organisms, made from a pure culture, which is used to rehydrate Sodium chloride 5g
Sodium sulfite 0.5 g
the enzymatic substrates. The metabolic end products produced Phenol red 0.17 g
during the incubation period are either revealed through Demineralized water to make 1000 ml
pH : 7.8
spontaneous colored reactions or by the addition of reagents.
NIN reagent Ninhydrin 7g
The fermentation tests are inoculated with an enriched medium 5 ml 2-methoxyethanol 100 ml
which reconstitutes the sugar substrates. Fermentation of TOXIC
carbohydrates is detected by a shift in the pH indicator. R60 : May impair fertility.
The reactions are read according to the Interpretation of R61 : May cause harm to the unborn child.
R10 : Flammable.
Reactions (Table 2) and the identification is obtained by referring R20/21/22 : Harmful by inhalation, in contact with skin
to the Analytical Profile Index or using the identification software. and if swallowed.
S53 : Avoid exposure (avoid contact with skin and
eyes, vapor inhalation and brutal superheating).
S45 : In case of accident or if you feel unwell, seek
REAGENTS medical advice immediately (show the label where
Kit contents (25 tests) : possible).
Voges- 40% aqueous KOH solution 89%
- 25 API 20 STREP strips Proskauer in demineralized water 11%
- 25 incubation boxes reagent: CORROSIVE
Potassium
- 25 ampules of GP Medium hydroxide R35 : Causes severe burns.
S24/25: Avoid contact with skin and eyes.
- 25 result sheets 30 ml
S26 : In case of contact with eyes, rinse immediately
- 25 swabs with plenty of water and seek medical advice.
- 1 package insert S36/37/39 : Wear suitable protective clothing, gloves
and eye/face protection.
S45: Incase of accident or if you feel unwell, seek
Additional products (not included in the kit) : medical advice immediately (show the label where
possible).
- Suspension Medium, 2 ml (Prod. No. 70700)
- Reagents : Ninhydrin (NIN) (Prod. No. 70490) Voges alpha-naphthol 6g
Proskauer Ethyl alcohol 100ml
Potassium hydroxide (Prod. No. V7053) reagent: VP2
VP2 or (Prod. No. 70430) 5 ml or
_-naphthol (Prod. No. V7054) alpha-naphthol 2.05 g
alpha-naphthol to be diluted with 95% ethyl alcohol 29ml
ZYME A (Prod. No. 70470) 30 ml
ZYME B (Prod. No. 70480) FLAMMABLE AFTER RECONSTITUTION
- Mineral oil (Prod. No. 70100) R21/22: Harmful in contact with skin and if swallowed.
- Sterile Pasteur pipettes S24/25: Avoid contact with skin and eyes.
- McFarland Standard #4 (Prod. No. 70900) ZYME A reagent Tris-hydroxymethyl-aminomethane 25 g
8 ml Hydrochloric acid (37 %) 11 ml
- API 20 STREP Analytical Profile Index (Prod. No. 20690) or Sodium lauryl sulfate 10 g
identification software (consult bioMérieux) H2 O 100 ml
- Ampule rack (Prod. No. 70200) ZYME B reagent Fast Blue BB 0.35 g
- Columbia blood agar plates 8 ml 2-methoxyethanol 100 ml
- Schaedler broth (optional) TOXIC
R60 : May impair fertility.
R61 : May cause harm to the unborn child.
Required laboratory equipment : R10 : Flammable.
R20/21/22 : Harmful by inhalation, in contact with skin
- 35-37°C incubator and if swallowed.
S53 : Avoid exposure - obtain special instructions
- Refrigerator (2-8°C) before use.
S45 : In case of accident or if you feel unwell, seek
- Bunsen burner medical advice immediately (show the label where
- Marker pen possible).
- Anaerobic jar
- Inoculating loop
STORAGE OF THE STRIPS AND MEDIA
The strips and media should be stored at 2-8°C until the
expiration date indicated on the packaging.
STORAGE OF THE REAGENTS
The reagents should be stored in the dark at 2-8°C (except
Potassium hydroxide and ZYME A which can be stored at 2-
30°C) until the expiration date indicated on the packaging. VP2
may be kept up to 1 month after the ampules have been opened
and the reagent transferred to the dropper-bottle. After
reconstitution, alpha naphthol should be stored at 2-8°C and may
be kept for up to 90 days, (or until the expiration date if this
comes first) : record the reconstitution date on the bottle label.
The ZYME A, ZYME B and NIN reagents may be kept for up to 1
month after the ampules have been opened and the reagents
transferred into the dropper-bottles, (or until the expiration date if
this comes first) : record the date opened on the bottle label.
The NIN and ZYME B reagents are very sensitive to
light : wrap the bottles in aluminum foil and only leave them out of
the refrigerator while being used. Do not leave them on the bench
for prolonged periods of time.
The NIN reagent is very sensitive to traces of water and air :
transfer the reagent into the dropper-bottle using a dry pipette
and keep the bottle tightly closed.
The ZYME B reagent is normally yellow in color. Dispose of the
reagent if any tint of pink (sign of deterioration) is observed.
At 2-8°C, the ZYME A reagent may form a precipitate which does
not affect any of the properties of the reagent and which may be
redissolved by gently heating.
USE OF THE REAGENTS
Allow reagents to come to room temperature (20-30°C) before
using.
1. ZYME A and ZYME B reagents :
• Open the ampule of reagent as indicated in the paragraph
"Warnings and Precautions" (ampule with dropper-cap).
• Dispense one drop of reagent.
• Carefully close the bottle after use and store it as indicated in
the paragraph "Storage of the reagents".
2. NIN reagent :
• Open the ampule of reagent as indicated in the paragraph
"Warnings and Precautions" (ampule with no dropper-cap).
• Take up the contents of the ampule using a completely dry
pipette and transfer this liquid into the dropper-bottle.
• Fit the dropper to the bottle.
• Dispense one drop of reagent.
• Carefully close the bottle after use and store it as indicated in
the paragraph "Storage of the reagents".
3. VP2:
• Open the ampule of reagent as indicated in the paragraph
Warnings and Precautions" (ampule with dropper cap).
• Dispense one drop of reagent.
• Carefully close the bottle after use an store it as indicated in the
paragraph "Storage of the reagents".
4. Alpha-naphthol :
• Reconstitute with 29 ml of 95% ethanol.
• Carefully close the bottle.
• Shake.
• Reagent can be used after active ingredient is completely
disolved.
• Dispense one drop of reagent.
• Carefully close the bottle after use an store it as indicated in the
paragraph "Storage of the reagents".
WARNINGS AND PRECAUTIONS
• For in vitro diagnostic use only.
• Qualified laboratory personnel should use aseptic technique
and established precautions for infectious agents.
• Do not pipette specimens or reagents by mouth.
• Do not use reagents past the expiration date.
• Do not allow reagents to come into contact with skin, eyes or
clothing.
• Do not interchange reagents or consumables between different
lot numbers.
• Upon removal from refrigerator, allow reagents to come to
room temperature (20-30°C) before using.
• Open ampules carefully as follows :
- Hold the ampule in one hand in a vertical position
(white plastic cap uppermost).
- Press the cap down as far as possible.
- Cover the flattened part of the cap with the upper
part of the thumb.
- Apply thumb pressure in an outward motion to
the flattened part of the cap to snap off the top of
the ampule inside the cap.
*For ampule with no dropper-cap :
- Carefully remove the cap.
*For ampule with dropper-cap :
- Turn the ampule upside down and maintain it in
a vertical position.
- Squeeze on the cap to transfer all the reagent
into the dropper-bottle.
• All inoculated products should be considered infectious and
handled appropriately.
• All patient specimens and microbial cultures are potentially
infectious and should be treated with universal precautions
(NCCLS M29-A: Protection of Laboratory Workeres from
Instrument Biohazards and Infectious Disease Transmitted by
Blood, Body Fluids, and Tissue: Approved Guideline. 1997).
• After completing test, reading and interpretation, all specimens,
spills and inoculated products must be autoclaved, incinerated
or immersed in a germicide prior to disposal.
• Interpretation of the test results should be made by a
competent microbiologist who should also take into
consideration the patient history, the source of the specimen,
colonial and microscopic morphology and, if necessary, the
results of any other tests performed, particularly the
antimicrobial susceptibility patterns.
• Any changes or modifications in the procedure may affect the
results.
INSTRUCTIONS FOR USE
Specimens and bacterial cultures should be considered infectious
and handled appropriately by trained and competent technicians.
Aseptic technique and usual handling precautions for the
bacterial group studied should be observed throughout this
procedure ; refer to Universal Precautions (NCCLS M29-A:
Protection of Laboratory Workers from Instrument Biohazards
and Infectious Diseases Transmitted by Blood, Body Fluids, and
Tissue : Approved Guideline. 1997).
For additional handling precautions, refer to Biosafety in
Microbiological and Biomedical Laboratories, HHS Publication
No. (CDC) 93-8395, 3rd Edition (May 1993), or to the regulations
of each country.
Selection of colonies
Once the microorganism to be identified has been isolated and
verified to be a member of the genus Streptococcus or related
genera shown in Table 3 (Gram, catalase test) :
• Note the type of hemolysis on the result sheet
(21st test).
• Pick a well-isolated colony (Note 1) and suspend it in 0.3 ml of
sterile water. Homogenize well.
• Flood a Columbia sheep blood agar plate (Note 2) with this
suspension (or aseptically swab the entire surface of the agar).
• Incubate anaerobically for 18-24 hours at 35-37°C.
NOTE 1 : Alpha-hemolytic streptococci and enterococci produce
sufficiently large colonies after 24 hours of incubation. For other
streptococci, it is preferable to select a colony after 48 hours of
incubation. For fastidious strains (producing minute colonies after
48 hours), the following procedure is recommended :
- Culture the colony in 1 ml of Schaedler broth at 35-37°C for 5
hours.
- Flood a Columbia sheep blood agar plate with the entire
culture. Remove any excess liquid.
- Incubate anaerobically for 18 hours at 35-37°C.
NOTE 2 : In the case of suspected pneumococci, it is advisable
to prepare 2 plates in order to obtain sufficient growth.
Preparation of the strip
• Prepare an incubation box (tray and lid) and distribute about 5
ml of distilled water or demineralized water [or any water
without additives or chemicals which may release gases (e.g.,
Cl2, CO 2, etc.)] into the honeycombed wells of the tray to create
a humid atmosphere.
• Record the strain reference on the elongated flap of the tray.
• Remove the strip from its packaging and place it in the tray.
Preparation of the inoculum
• Open an ampule of Suspension Medium (2 ml) as indicated in
the paragraph "Warnings and Precautions" (ampule with no
dropper cap) or use any tube containing 2 ml of distilled water
without additives.
• Using a swab, harvest all the culture from the previously
prepared subculture plate. Make a dense suspension with a
turbidity greater than 4 McFarland.
Inoculation of the strip
• In the first half of the strip (tests VP to ____ ADH) distribute the
suspension with a sterile pipette, avoiding the formation of
bubbles (tilt the strip slightly forwards) :
- For the tests VP to LAP : distribute approximately 100 µl into
each cupule (3 drops with a Pasteur pipette ).
- For the ____
ADH test : fill the tube only.
• In the second half of the strip (tests RIB
___ to ______
GLYG ) :
- Open an ampule of GP Medium as indicated in the
paragraph "Warnings and Precautions" (ampule with no
dropper-cap) and transfer the rest of the suspension into it
(appr. 0.5 ml). Mix well.
- Distribute this new suspension into the tubes only.
• Fill the cupule of the underlined tests (ADH
____ to ______
GLYG ) with
mineral oil to form a convex meniscus.
• Place the lid on the tray.
• Incubate at 35-37°C for 4 hours to obtain a first reading and for
24 hours to obtain a second reading if this is required.
Reading of the strip
After 4 hours of incubation :
• Add the reagents :
- VP Test : 1 drop of each of Potassium hydroxide and VP2 or
alpha-naphthol.
- HIP Test : 2 drops of NIN reagent.
- PYRA, _GAL, _GUR, _GAL, PAL and LAP Tests : 1 drop of
each of ZYME A and ZYME B reagents.
• Wait 10 minutes, then read the reactions by referring to the
Interpretation of Reactions Table (Table 2). If necessary,
expose the strip to a strong light (10 seconds with a 1000 W
lamp) to decolorize any excess reagents in the tubes PYRA to
LAP.
Reincubation is necessary :
- if the profile cannot be found in the API 20 STREP Analytical
Profile Index
- if the profile is given with the following note :
IDENTIFICATION NOT VALID
BEFORE 24 HOURS OF INCUBATION
After 24 hours, reread the reactions ESC, ____ ADH, and RIB
___ to
GLYG . Do not reread the enzymatic reactions (HIP, PYRA,
______
_GAL, _GUR, _GAL, PAL, LAP) and VP. Record the reactions on
the result sheet.
Identification
Identification can be obtained :
• using the Analytical Profile Index : the pattern of the reactions
obtained must be coded into a numerical profile.
On the result sheet, the tests are separated into groups of 3 5 240 550 / 5 240 770 Streptococcus mutans
and a number 1, 2 or 4 is indicated for each. By adding the
numbers corresponding to positive reactions within each NOTE : The hemolytic reaction constitutes the 21st test ; beta-
group, a 7-digit profile number is obtained. hemolysis is considered as positive with a numerical value of 4.
All other hemolytic reactions are considered as negative with a
To obtain information on any profile not listed in the codebook, numerical value of 0. Nevertheless, this test may be of
call the Voice Response System at 800-645-7056. discriminant value for the identification of certain species.
• using the identification software.

QUALITY CONTROL
The media, strips, and reagents are systematically quality controlled at various stages of their manufacture. For those who wish to
perform their own quality control tests with the strip, it is recommended that the following stock cultures be used, to obtain the results
below : Table 1

VP HIP ESC PYRA _GAL _GUR _ GAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG
1. – – – – – + – + + + + – – + + – – – + +
2. + V + + + V + – V + + + + – + + + + + –
3. + + + V V + – V + + + – + + + + + + – –
4. V V + V V – – – – – – – + – – + – + + –

1. Streptococcus equi ssp zooepidemicus ATCC 700400 3. Streptococcus uberis ATCC 700407
2. Enterococcus gallinarum ATCC 700425 4. Aerococcus viridans ATCC 700406
ATCC : American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, USA.
• Inoculum adjusted to between 4.5 and 5.5 McF.
• Profiles obtained after : - 4 hours of incubation for tests VP to LAP
- 24 hours of incubation for tests ____
ADH to _____
GLYG
• Strains cultured on Columbia sheep blood agar

DISPOSAL OF USED MATERIAL LIMITATIONS

After use, all ampules, swabs, pipettes, strips and incubation
boxes should be autoclaved, incinerated or immersed in a
disinfectant for decontamination prior to disposal.
The API 20 STREP system is intended uniquely for the
identification of those species included in the database (see
Identification Table (Table 3) at the end of this package insert). It
cannot be used to identify any other microorganisms or to
exclude their presence.

RANGE OF EXPECTED VALUES

The API 20 STREP test produces color reactions which make
positive and negative identification possible. Absolute values are
not obtained.
 INTERPRETATION OF REACTIONS

Table 2
TESTS SUBSTRATES REACTIONS/ENZYMES RESULTS

NEGATIVE POSITIVE

Potassium hydroxide(1 drop) +VP2

or alpha-naphthol (1drop) / wait 10
min

VP Pyruvate Acetoin production Colorless Pink-Red (3)

NIN (2 drops) / wait 10 min

HIP Hippurate Hydrolysis Colorless/Pale blue Dark blue/Violet

4 hrs. 24 hrs. 4 hrs. 24 hrs.

ESC Esculin _-glucosidase Colorless Colorless Black Black

Pale yellow Pale yellow Grey
Light grey

ZYME A (1 DROP) + ZYME B (1 DROP) / 10 MIN (PYRA to LAP) (1)

if necessary, decolorize with intense light

PYRA Pyrrolidonyl-2-naphthylamide Pyrrolidonyl arylamidase Colorless or very pale orange Orange

_GAL 6-Bromo-2-naphthyl
_-D-galactopyranoside _-galactosidase Colorless Violet

_GUR Naphthol AS-BI

_-D-glucuronate _-glucuronidase Colorless Blue

_GAL 2-naphthyl- _-D-

galactopyranoside _-galactosidase Colorless or very pale violet Violet

PAL 2-naphthyl phosphate Alkaline phosphatase Colorless or very pale violet Violet

LAP L-leucine-2-naphthylamide Leucine arylamidase Colorless Orange

ADH
____ Arginine Arginine dihydrolase Yellow Red

4 hrs. 24 hrs. 4 hrs. 24 hrs.

RIB
___ Ribose Acidification Red Orange/Red Orange/Yellow Yellow
ARA
____ L-Arabinose Acidification Red Orange/Red Orange/Yellow Yellow
MAN
____ Mannitol Acidification Red Orange/Red Orange/Yellow Yellow
SOR
____ Sorbitol Acidification Red Orange/Red Orange/Yellow Yellow
LAC
____ Lactose Acidification Red Orange/Red Orange/Yellow Yellow
TRE
____ Trehalose Acidification Red Orange/Red Orange/Yellow Yellow
INU
___ Inulin Acidification Red Orange/Red Orange/Yellow Yellow
RAF
____ Raffinose Acidification Red Orange/Red Orange/Yellow Yellow
AMD
____ Starch (2) Acidification Red Orange/Red Orange/Yellow Yellow

GLYG
_____ Glycogen Acidification Red or Orange Bright yellow

(1) During a second reading after 24 hours of incubation, a deposit may be noticed in the tubes where the ZYME A and ZYME B reagents have been added.
This phenomenon is normal and should not be taken into consideration.

(2) The acidification of starch is frequently weaker than that of other sugars.

(3) A pale pink color obtained after 10 minutes should be considered negative.
 0
api 20 Strep 07625 B - 08/97

RECOMMENDED METHODOLOGY

- Cocci
- Gram +
- Catalase −

Blood agar

Columbia blood agar

24:00 02 37°C

> 4 McF

Suspension Medium 2 ml

VP ADH

ADH

GP Medium

RIB GLYG

RIB GLYG

4:00 37°C

24:00 37°C VP : Potassium hydroxide +

VP2 or alpha naphthol
HIP : NIN
api 20 STREP PYRA LAP : ZYME A + ZYME B

+ - + - + -
 IDENTIFICATION TABLE
Table 3
% of positive reactions after 4/24 hrs. at 35-37°C

API 20 STREP V6.0 VP HIP ESC PYRA AGAL BGUR BGAL PAL LAP ADH RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG HEM

Aerococcus viridans 1 13 50 96 54 33 16 37 1 5 1 83 33 85 70 83 99 33 41 70 33 1

Aerococcus viridans 2 15 70 50 76 10 20 25 1 5 5 25 1 35 2 70 89 1 5 24 1 5

Aerococcus viridans 3 22 88 99 40 85 48 14 14 1 1 8 2 82 5 91 99 37 99 14 1 1

Alloiococcus otitis 0 25 0 100 0 3 100 1 90 0 0 0 0 0 0 20 0 0 0 0 0

Enterococcus avium 99 60 99 94 15 0 24 1 99 0 99 40 100 95 95 99 1 40 15 0 1

Enterococcus durans 100 43 100 97 32 2 76 0 91 100 99 15 2 0 84 76 0 0 56 0 18

Enterococcus faecalis 99 46 99 97 1 0 21 4 99 94 98 0 98 92 92 100 0 0 96 2 1

Enterococcus faecium 94 43 99 95 42 1 90 3 97 93 85 70 78 18 84 98 26 9 73 3 1

Enterococcus gallinarum 99 99 100 100 95 45 99 0 99 99 99 100 99 1 100 100 99 99 83 20 0

Gardnerella vaginalis 0 95 0 0 0 1 53 0 99 0 46 6 1 0 1 0 0 0 73 53 0

Gemella haemolysans 25 0 0 70 0 0 1 84 40 1 1 0 20 10 5 2 0 0 10 5 1

Gemella morbillorum 3 0 0 35 0 0 10 35 86 4 5 0 1 0 1 11 3 1 16 5 0

Lactococcus lactis ssp cremoris 98 15 41 1 13 0 41 4 89 0 27 0 17 0 96 30 0 20 25 0 0

Lactococcus lactis ssp lactis 90 40 99 35 3 0 35 3 96 95 95 15 45 1 72 87 4 5 90 3 1

Leuconostoc spp 91 1 60 5 55 0 65 2 70 10 37 35 29 4 35 65 0 42 11 0 0

Listeria spp 97 79 98 0 0 0 0 0 85 0 6 0 0 0 49 92 1 1 72 0 26

Abiotrophia adiacens 0 0 10 80 0 25 0 0 99 0 0 0 0 0 0 0 0 0 0 0 0

Abiotrophia defectiva 25 0 15 99 100 0 100 0 92 0 0 0 0 0 99 100 5 93 99 0 0

Streptococcus acidominimus 1 95 4 13 1 66 30 60 96 18 17 0 42 10 70 65 0 0 10 0 0

Streptococcus agalactiae 100 99 1 1 4 79 1 96 99 99 98 0 1 1 50 87 0 1 35 4 75

Streptococcus anginosus 100 0 100 0 44 0 1 99 100 100 0 0 33 0 99 88 0 44 97 0 37

Streptococcus bovis I 97 2 100 2 71 4 14 0 97 0 2 13 86 0 100 90 63 90 100 90 0

Streptococcus bovis II 1 95 4 97 1 86 1 17 0 100 1 0 14 0 0 93 30 61 99 73 65 0

Streptococcus bovis II 2 86 4 100 13 85 88 94 0 100 13 0 1 0 0 99 99 13 72 40 13 0

Streptococcus canis 0 1 25 4 95 1 80 100 100 100 100 0 0 0 99 1 0 1 99 0 100

Streptococcus constellatus 100 1 27 0 0 0 5 99 100 100 0 0 0 0 10 72 0 0 12 0 61

Streptococcus dys.ssp dysgalactiae 0 0 1 1 1 99 0 100 99 100 99 0 1 50 86 100 0 1 99 30 2

Streptococcus dys.ssp equisimilis 0 1 25 1 1 99 1 99 100 97 97 1 1 1 45 99 0 1 98 40 94

Streptococcus equi ssp equi 1 0 1 0 0 100 0 100 100 100 0 0 0 0 0 1 0 0 100 100 100

Streptococcus equi ssp zooepidemicus 0 1 15 0 0 100 1 99 100 99 85 0 0 99 100 0 0 0 99 99 99

Streptococcus equinus 100 0 95 0 28 0 1 1 100 0 0 0 30 0 25 7 25 15 17 10 0

Streptococcus group L 0 75 1 0 0 100 1 100 100 100 100 0 0 0 75 100 0 0 100 98 94

Streptococcus intermedius 100 0 87 0 0 0 44 99 100 100 0 0 0 0 99 99 3 3 99 0 40

Streptococcus mitis 1 1 0 3 1 21 0 25 35 99 19 14 1 0 1 94 7 3 26 67 5 0

Streptococcus mitis 2 0 0 3 0 31 0 35 50 100 99 1 0 1 0 100 1 1 31 84 0 0

Streptococcus mutans 99 0 99 1 64 0 1 1 100 18 0 0 99 90 90 100 81 81 1 0 1

Streptococcus oralis 0 0 1 1 50 0 46 72 100 5 1 0 1 0 99 32 1 72 96 0 0

Streptococcus pneumoniae 0 0 39 60 70 3 79 3 100 57 3 0 0 0 99 98 64 87 84 10 1

Streptococcus porcinus 100 5 99 1 19 99 1 97 97 100 98 0 88 88 83 99 0 0 50 0 100

Streptococcus pyogenes 0 1 5 98 0 15 0 100 100 99 0 0 8 1 99 98 0 1 61 22 98

Streptococcus salivarius ssp salivarius 85 0 98 1 8 0 70 20 100 0 0 0 5 1 86 67 34 88 74 1 1

Streptococcus sanguis 0 1 42 0 63 0 1 5 100 90 0 0 1 48 83 98 33 55 67 0 0

Streptococcus suis I 0 1 82 53 80 94 76 1 100 91 0 0 7 0 94 100 75 0 100 89 0

Streptococcus suis II 0 1 70 41 91 91 52 3 100 95 0 0 3 1 99 98 63 93 99 96 2

Streptococcus uberis 99 98 100 35 10 86 5 30 100 98 99 0 99 98 99 100 89 10 50 20 0

 BIBLIOGRAPHY
1. APPELBAUM P.C., CHAURUSHIYA P.S., JACOBS M.R.,
DUFFETT A.
Evaluation of the Rapid Strep System for Species
Identification of Streptococci.
(1984) J. Clin. Microbiol., 19, 588-591.
2. BALL L.C., COLMAN G.
A Comparison of Conventional Methods and API Galleries
for the Identification of Streptococci.
(1982) International Meeting on Streptococci and
Streptococcal Diseases, LUND SWEDEN, 41-42.
3. BANNISTER M.F., BENSON C.E. and SWEENEY C.R.
Rapid Species Identification of Group C Streptococci
Isolated from Horses.
(1985) J. Clin. Microbiol., 21, 524-526.
4. COLMAN G., BALL L.C.
Identification of Streptococci in a Medical Laboratory.
(1984) J. Appl. Bact., 57, 1-14.
5. FACKLAM R.R., RHODEN D.L., SMITH P.B.
Evaluation of the Rapid Strep System for the Identification
of Clinical Isolates of Streptococcus Species.
(1984) J. Clin. Microbiol., 20, 894-898.
6. HUMAN R.P. and TILLOTSON G.S.
Identification of Gardnerella vaginalis with the API 20 Strep
System.
(1985) J. Clin. Microbiol., 21, 985-986.
7. KLOOSTERMAN R.E., CULLEN K.D.
Comparison of Two Commercial Systems for the Rapid
Identification of Streptococci.
(1984) ASM ST. LOUIS C198.
8. MacGOWAN A.P., MARSHALL R.J., REEVES D.S.
Evaluation of API 20 STREP System for Identifying Listeria
Species.
(1989) J. Clin. Path., 42, 548-550.
9. RUOFF K.L., KUNZ L.J.
Use of the Rapid STREP System for Identification of
Viridans Streptococcal Species.
(1983) J. Clin. Microbiol., 18, 1138-1140.
10. TILLOTSON G.S.
An Evaluation of the API 20 Strep System.
(1982) J. Clin. Path., 468-471.
 Fabricant / Manufacturer bioMérieux SA

bioMérieux SA bioMérieux, Inc.

au capital de 77 421 420 F 595 Anglum Road Hazelwood,
673 620 399 RCS LYON MO 63042-2320 / USA
69280 Marcy-l’Etoile / France tel. (1) 314-731-8500 / fax (1) 314-731-8800
tel. (33) 4 78 87 20 00 / fax (33) 4 78 87 20 90 Imprimé en / Printed in USA