Received:

.: 16th International Symposium on

Aromatic Compounds
November 4 - 7p 1997
Charlotte, North Carolina, USA
Jan 15 2009 01:45Dm
Presentation Session: 11 Nov 7 0950-1010
Mackerer
Mutagenicity and PAC content of seal coatings for asphalt pavement
MUTAGENICITY AND PAC CONTENT OF SEAL COATINGS FOR ASPHALT
PAVEl\.1ENT. C. R. MACKERER
1
T. A. ROyl and G. R. BLACKBURN
2
lproduct Stewardship and Toxicology, Mobil Bu.ciness Resources Corporation, Paulsboro,
Nl 08066 and 2PetroLabs, Inc-
7
IvYland, PA 18974
We have evaluated the mutagenicity and polycyclic aromatic compound (PAC) comem: of
12 water emulsion products available to cOl1SUIIlers fOT the sealing of asphalt pavemtom.
Small pails of product (5-6 gallons each) were pun:hased at local department storcs_
According to the labels on the pails there were 11 coal tM emulsions (samples 1 - 10 and
12) and one asphalt emulsion (sample 11). The emulsions were broken by cc:posing the
pails to multiple freeze-thaw and the ceDlIill portion of the solids fraction was
sampled and blotted dry. 20 g of each product was for PAC
analysis and testing in the modified Ames mutagenicity assay (ASTM E1687J9S). For
samples 1 - l2, the level of solids (i.e. DCM insoluble mnerial) ranged from 56% to 91%
(mean, 72.5%). The DCM soluble material was tested in ASTME1681J95 where samples
1 - 10 and 12 produced very high mutagenicity index (Ml) values (ranging from 275 to
657 (mean, 445); sample 11 produced an MI of.only 0.6. The DCM soluble fractions of
sampiE3 1 - 10 and 12 contained similar amounts of 3-7 ring PAC ranging from 43% to
57% (mean, 50.9%); sample 11 contained an estimated 3-7 ring PAC level of 2.6%_
GeMS indicated that samples 1 - 10 and 12 contained coal tar obtained from the
same source, but also, that sample 11 contained a low level of the same tar. In.
addition., sample II did not comain pentane insoluble components such as asphaltenes that
would indicate the presence of asphalt. The very high MI values seen fo[" samples 1 - 10
and J2 suggest that individuals who may have prolonged and repeated dennal contact with
products such as these may also have an elevated risk for skin cancer.
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CompDsition Labellnformation
Sample Product Name Component CASNo. Toxicology First Aid
No.
t Blacktop Driveway Relined coal tu 65996909 May caUBe skin irritation.
Coating Clay 131258-7
Silica 14808-60-1
Water 7732-18-5
I 1 Polymer Fortified Asphalt 8052-42-4
Driveway SealerlFiHer Clay 1332-58-7 No skin irritants.
-, -
Silica J4808-60.. 7 Ecologically safe.
Ethylene-vinyl 2506710-0
Acetate c o p o ~ y m e r
Water 7732-18-5
2 Dri\leway Sealer Premium- Contains no solvents May cause irritation. In case of skin contact
Rubberized Coa] ter based tinse with water.
4 Driveway Contairts no solvents May cause irritation. In case of skin contact
Sealer/Resurfacer. Coal tar based rinse thoroughly with
Polymer Fortified Sealer, water.
Rubberized - Te.l(lurized
,
3 Rubberized Blacktop Contains coallar pit.ch May cause skin irritation. In case of contact with
Sealer Coal tar 8007-45-2 Harmful if swaJlowed. eyes or skrn, flush with
K.aolin clay 1332..58-7 water.
Amorphous silica 7631..86-9
7 Driveway Sealer, Blacktop Contains coal tar pitch Eye and skin irritant. [n case of contact with
Tar Emulsion Coal t.3.r 8007-45-2 Harmfullfswallowed. eyes or skin. flush whh
Kaolin clay 1332-58-7 water.
5 Drive\vay Sealer Filler. Latex, finely graded ... flusI1 with water [i.e.
Rubberized. Latex aggratales and mineral eyes and mouth] '.'
formula. coal tar,
Refined coal tar 65996-90-9
--._.
Cla.y 1332-58-7
Silica 14808-60-7
'i\TaIer 7732-J 8-5
Page 1
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Composition Label Infonnation
Product Name Component CAS No. Toxicology First Aid
No,
B Coal Tar - Sealer Filler. Coal tar ... flush with water (Le.
Driveway Sealer with Sand eyes and mouth]...
Sand Added
12 Coal Tar Driveway Sealer Refined coal tar 5599690.. 9 If substance gets in eyes
Clay 1332-58-7 or maut h, flush with
Smca 14808.. 60-7 water...
Water 7732.. 18-5
6 Rubberized Driveway Coal tar cat:ne s,kin irritation, [f swallowed. do not
Sealer lnduce vomiting.
10 Rubberized Driveway Coal tar May cause skin irritatIon. If swallowed, do not
Sealer induce vomitjng.
9 Driveway Sealer) Latex Coal tar pilch 8007-45-1 Harmful or fatal if ,..if swallowed, induce
Rubber Formula (Pilch coal tar swallowed. skin, vomiting. In case of eye
Base) Clay 1332-58.. 7 respiratory irritant. contact flush with
Water
_..-
-p1enty1Jfwann-wat er.
Sand 14808-60-1 or stopped
brealhing - administer
oxygen, start
resuscitation.
"Sources ofproducts: Hecltinger
l
Ox.ford Valley, PA; Sears Roebuck, TrenLon
J
Channel Lumber. Lawren:Jeville
J
NJ; and
Gros!;man, Lawrenceville) NJ.
Page 2
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Ames Test
Liver
Mineral
MelaboUztng
all Ames Tester 5-9 Mixture
(Per Assay) BacterIa (Per Plate)
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Add 3 ml of Strain Prepare a-Fold
Q.
to
TAgS
"0:1'"
Cyclohexane to
(400 Arocior

0
2. ml of all
1254 Induced. Syrian
CD
0
V
Golden Hamster LIver

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Mix
5-9 .......
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Add 10 DMSO
(0

V
V
Add 2-Ford (B mM)
MIx Every 5 Min.
NADP Cofaclor
for 30 Min. V
V
Add Standard Amounts
Collect 5 ml Aliquot
of Other Components
of Lower DMSO L:ayer
of S9 Mlxture
Free of
Slore In Dark at
4C Until Assayed
"'C Combine Acpropriale Volumes
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In uspenslon
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0
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Cl)

Incubate 20 Min. at 37
w
C
V
Pour Into Petri Plate
V
Incubate 48 Hrs.. at 37C
V
Count Revertant Colonies 930354 7
2 x 2 Table for Tumor Incidence and Mutagenicity
Indices for 55 Oils'
< 2 Mice
With Tumors
Boiling Above 50[f F
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p= .0001
GC/FID Chromatogram of a High Percentage PAC Petroleum
Distillate Extract With Overlay of PAH Standard Chromatogram
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930354 23
Carcinogenicity vs. 3-7 Ring PAC Content (%)
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37 Ring PAC
< 10/0
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~ 1%
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< 2.Mice .
Wit'h Tumors
2
---------
2
~ 2 Mice
With rumors
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23
C:arcinogenicity of Petroleum Oil is Dep'endenl Upon the
P ~ r e s e n c e and Concentration of PAC
930354 33
Correlation Between 37 Ring Polycyclic Aromatic Compounds
(PAC) and Mutagenicity In the Modified Ames Test for 104
Petroleum-Derived Oils
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% 37 R!ng PNA
930354 10
Received:
Jan 15 2009 01:47pm
Fractionation of Drivevlay Sealer Emulsions
120g of samliliiJ DeMextractidD
determine wtOA. DeM
solubles
determine wtOA. '''non-
as haltcnes" (solubles
reconstitute in
cyclohexane
" - - ~ - ~ ~ - - - r
extract DMSO soluble
determine wt%
DMSO soillbles/g
DCM solubles'
assayDMSO
soIubles etC-Fill
detennine wt%
1-7 ring aromatics/g
DCM solubles
determine wtO/o solids
determine wt%
entane-asphalteneslg
DeM solubles
. assayDMSO
solubles by GC-MS
dnray.doc
DCM Extractable Materllt In 12 Driveway SI.ler.
S a [ n p ( ~ No.
Fractlonatton of the OeM So'ub1e Components
B S8mple11
p,P'J lin.
fnsoJubit
99....-------
Pol.,
p."flln
Htphrhtm/t
F r I ~ f l o n
Pentane
Extraction
DMSO
Extraction
Modlned Ames Test Results for 12 Driveway Sea1ers
BOO .
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675 CD
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2 :3 .{ 5 6 1 tl 9 '0 11 t2
Sample No.
1 - 7 Ring Aromatics Present in the DMSO Fraction of Samples 1 - 12
(Expressed as %of Total)
Totaf Polycyclic Aromatie Compounds
SAMPLE 11
8. Polycyclic Aromatics
SAMPLES 1-10
1
12
1
Aromatics Found ~ n Ring Groups
A. AU Aromatics
SAMPLES 1 12
35
33.1
"O.B
30
~ 25
t'1
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~ 20
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15
10
5
1,32
:1:.51
0
1 2 3 4 5 6
Received:
Jan 15 2009 01:49pm
Comparison ofGC-MS Results for Coal Tar Based Driveway Sealers
COALTAII1
1.. '2
20.96
J].66
11.. 50


.47
Area ,
Gaoui' I
GROUP II
GROUP 'II%
GROUP N
GROUP V
GROUP VI
GROUP VIJ:
GC
t'eak Na.aMI
-19
COA1."'I'.I\B1
58-

GC aults:
Peak NIIIDII'
Area ,
48
alOOf I
GN)OP IX 11..1.0
39
GROUP 1:I1 31.1Z
GROtn'IV 11.26
29
GROUP V lL.'.
-
GROUP VX 10.18
,.....
tra
GIWUP 'ILl: 5.24
COAL"1'A.Jl12
S8
raY
.a
30
21
161
9
-li
Q
UI 21 38
PllfI.I.... ,
ClOUp I
. GRQQ'P 'II:
GROUP XIX
GlU)UlI rI
GRQOP V
GRQOP VI
GRCOl' Vl:X
1.. 9
19.. 9
32.8
11 .. E
12.:1
lO.. E
t. i
Comparison of GC-MS Results for Products 11 and 12
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Com par eRe p 0 r t .,

COALTARll EK Ce> lnJ 1 Ch 1
15
mV
10
5
,
In..J 1 Ch 1

COALTARJ2 EX ( 0)
2513"
2ae
158
lee-
5e
.........
o 10
Mtnutes
mV
t.
Mutagenicity MI of AsphaJt and Coal Tar Pitch
'"0
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1100--
1000-
900
BOO-
700--
600-
:i
500-'
400-
1,5
300-
200-
100-
C
Coal Tar Roofing
Pitch Asphala1
(Type I) Type In
l.
Roofing
Asphalt
TypelU
,
r-F 0,5