Lactose added to identical cultures of Escherichia coli

Start

9:55:00
10:00:00
10:05:00
10:10:00
10:15:00
10:20:00
10:25:00
10:30:00
10:35:00

End

10:25:00
11:00:00
11:35:00
12:10:00
12:45:00
13:20:00
13:55:00
14:30:00
15:05:00

Time lapse

0:30:00
1:00:00
1:30:00
2:00:00
2:30:00
3:00:00
3:30:00
4:00:00
4:30:00

glucose
mol/l

26
38
48
57
65
74
81
86
90

glucose
mol/min

0.87
0.63
0.53
0.48
0.43
0.41
0.39
0.36
0.33

1.00
0.90
0.80
0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
0:30:00

glucose mol/min

1:00:00

1:30:00

2:00:00

2:30:00

3:00:00

3:30:00

4:00:00

4:30:00

4:30:00

Activities of 10 Mice in 2 different cages

Sleep

Eat

Run

Cage A

47

37

16

Cage B

60

21

19

100%

120

90%
100

80%
70%

80
Cage B
60

Cage A

40

60%
50%
40%
30%
20%

20

10%

0%
Sleep

Eat

Run

Cage B
Cage A

Sleep

Eat

Run

Plate ID
8877p58a StdDev
8877p58b StdDev
8696p08a StdDev
8696p08b StdDev
8697p58b StdDev
Grand StdDev

50 ng/mL %CV 25 ng/mL

1.48772757
0.1414214
1.87860764
1.0242884
2.85817541
1.6938615
1.79722008
1.1518102
2.21114601
0.1414214
3.42779275
2.2181073

%CV

3.5

2.86

2.5
2.21

1.88
1.80
1.69
50 ng/mL

1.5

1.49

25 ng/mL
1.15
1.02

0.5
0.14
0

0.14

8877p58a StdDev

8877p58b StdDev

8696p08a StdDev

8696p08b StdDev

8697p58b StdDev

50 ng/mL

1.487727574

1.87860764

2.858175409

1.797220076

2.211146008

25 ng/mL

0.141421356

1.024288371

1.693861466

1.15181017

0.141421356

Weight Bins Frequency

120
1015
130
1022
140
1040
150
1055
160
1050
170
1046
180
1042
190
1028
200
1011
1200
1000
800
Weight Bins

600

Frequency
400
200
0
1

120
130
140
150
160
170
180
190
200

Frequency
1015
1022
1040
1055
1050
1046
1042
1028
1011

Frequency
1060
1050
1040
1030
1020

Frequency

1010
1000
990
980
120

130

140

150

160

170

180

190

200

Day1
Day2
Day3
Day4
Day5
Day6
Day7
Mean

Strain1 Strain2 Strain3 Strain4

1.24
1.78
1.45
1.92
1.30
2.15
2.41
2.04
2.04
2.99
2.64
2.10
2.43
3.90
3.18
2.86
3.38
4.48
3.72
3.26
3.86
5.39
4.59
3.58
4.01
5.43
5.00
4.42
2.61
3.73
3.28
2.88

0
Strain1

Strain2

Day1
Day2
Day3
Day4
Day5
Day6
Day7
Mean

Strain2

Strain3

Strain4

10
15
20
25
30
35
40
45
50
55
60
65
70

Frequencies Norm.Distr.
0
0
0
1
3
2
5
5
10
10
11
15
21
19
19
19
13
15
5
9
5
4
4
1
0
0

25

20

15

10

0
10

15

20

25

30
Norm.Distr.

35
Norm.Distr.

40

45
Frequencies

50

55

60

65

70

Z
% 95%
-3.00 0%
-2.75 0%
-2.50 0%
-2.25 1%
-2.00 1%
-1.75 2% 2%
-1.50 3% 3%
-1.25 4% 4%
-1.00 5% 5%
-0.75 7% 7%
-0.50 8% 8%
-0.25 9% 9%
0.00 10% 10%
0.25 10% 10%
0.50 9% 9%
0.75 8% 8%
1.00 7% 7%
1.25 5% 5%
1.50 4% 4%
1.75 3% 3%
2.00 2% 2%
2.25 1%
2.50 1%
2.75 0%
3.00 0%

12%

10%

8%

6%

4%

2%

0%
-3.00 -2.75 -2.50 -2.25 -2.00 -1.75 -1.50 -1.25 -1.00 -0.75 -0.50 -0.25 0.00 0.25

0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 2.00 2.25 2.50 2.75 3.00

Xconcentration
Yabsorption
0.003
2.5
0.003
2.9
0.005
2.9
0.010
3.3
0.014
3.3
0.019
3.3
0.029
4.2
0.040
4.2
0.057
5.8
0.063
6.0
0.090
8.9
0.125
23.3
0.180
59.2
0.250
89.0
0.300
96.1
0.425
99.9
0.500
100.0

120

100

80

60

40

20

0
0.00

0.05

0.10

0.15

0.20

0.25

0.30

0.30

0.35

0.40

0.45

0.50

Plate1
Plate2
Plate3
Plate4
Plate5

Day0
670
719
769
818
867

Day1
163
164
172
182
189

Day2
150
153
155
178
182

Day3
115
116
122
137
140

1000
900
800
700
600
500
400
300
200
100
0
Plate1

Plate2
Day0

Day1

Plate3
Day1

Day2

Plate4
Day3

Plate5

900
800

700
600
Day0

200
500

Day1
400
180

Day2
Day3

300
160
200
140
100
120
0
100

Day0
Plate1

Plate2

Plate3

Plate4

Plate5

Day1
Day2

80

Day3

60
40
20
0
Plate1

Plate2

Plate3

Plate4

Plate5

Bins Frequencies Norm.Distr. Cumulative

10
0
0
0
15
0
1
1
20
3
2
3
25
5
5
8
30
10
10
18
35
11
15
33
40
21
19
52
45
19
19
71
50
13
15
86
55
5
9
95
60
5
4
99
65
4
1
100
70
0
0
100

25

20

15

10

0
10

15

20

25

1.2

0.8

0.6

0.4

0.2

0
30

35

40

45

50

55

60

65

70

2
10
15
20
25
33
39
45
55
70

Near Point Foc. Power

in meters

0.071
0.077
0.083
0.091
0.100
0.125
0.167
0.250
0.500
1.000

1.2

dioptre (=1/NP)

14
13
12
11
10
8
6
4
2
1

1.0
0.8
Near Point

Age

0.6
0.4
0.2
0.0
0

10

20
Near Point

30
in meters

40
Foc. Power dioptre (=1/NP)

16
14

10
8
6
4
2
0
50

60

Foc. Power dioptre (=1/NP)

70

80

Focal Power

12

Strain1
Strain2
Strain3
MEAN
SD

1 week 2 weeks 3 weeks MEAN

0.45
0.55
0.70
0.57
0.30
0.34
0.39
0.34
0.32
0.37
0.43
0.37
0.36
0.42
0.51
0.08
0.11
0.17

SD
0.13
0.05
0.06

0.80
0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
1 week

2 weeks
Strain1

Strain2

Strain3

2 weeks
Strain3

3 weeks
MEAN

Cholesterol Measurements
Afr.Amer.

Caucasians Hispanics

Nat.Amer.

180

161

172

157

183

163

173

158

186

165

174

159

189

167

175

160

192

169

176

161

195

171

177

198

173

178

Nat.Amer.

159

Hispanics

175

Caucasians

167

Afr.Amer.

192

201
204
Afr.Amer.

Caucasians Hispanics

Nat.Amer.

192

167

175

159

Mean

8.22

4.32

2.16

1.58

SD

5.37

3.20

1.60

1.39

1.96 SE

150

155

1.39

155

160

165

170

175

180

185

190

195

200

200

Hb% RBCount
80
5.1
86
5.4
92
6.7
93
7.3
96
6.5
99
7.2
103
7.5
108
7.7
117
8.5

9.0
8.5
8.0
7.5

103, 7.5
93, 7.3

99, 7.2

7.0
92, 6.7
6.5

96, 6.5

6.0
5.5

86, 5.4
80, 5.1

5.0
80

85

90

95

100

105

117, 8.5

108, 7.7

110

115

120

20.0

18.0
16.0
14.0
12.0
10.0
8.0
6.0
4.0

2.0

Plate18

Plate17

Plate16

Plate15

Plate14

Plate13

Plate12

Plate11

Plate10

Plate9

Plate8

Plate7

Plate6

Plate5

0.0
Plate4

75%
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3
12.3

Plate3

25%
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9
7.9

Plate2

50 ng/mL
9.7
10.7
11.3
7.9
9.5
7.9
5.2
6.3
18.4
13.3
14.3
11.7
14.9
12.1
13.0
10.6
9.5
9.5
5.2
6.3

Plate1

Plate1
Plate2
Plate3
Plate4
Plate5
Plate6
Plate7
Plate8
Plate9
Plate10
Plate11
Plate12
Plate13
Plate14
Plate15
Plate16
Plate17
Plate18
Plate19
Plate20

Plate20

Plate19

Plate18

Hb%
72
76
79
82
86
87
87
88
90
90
90
90
92
93
94
94
94
95
95
96
96
98
102
104
108

RBCount
55
56
61
63
64
65
65
66
68
69
70
72
75
76
78
79
80
81
81
82
83
88
90
92
94

Median X
92.0
92.0
X
60
120

100

50.0
100.0
Median Y
75
75

R
B
C
o
u
n
t

95

90
85
80
75

70
65
60
55
50
60

70

80

90
Hb%

100

110

120

Generation
0
1
2
3
4
5
6
7
8
9
10

2.5
2
5
13
31
78
195
488
1,221
3,052
7,629
19,073

Generation Max. Capacity

0
1,221
7
1,221
7
1 if exponential
Generation Opt.Capacity
0
195
5
195
5
1

100,000

Series1
Max Capacity

10,000

Optimum Capacity

1,000

100

10

1
0

10

Fraction
Undissoc.
Acid
0.01
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
0.60
0.65
0.70
0.75
0.80
0.85
0.90
0.95
0.99

4.75

pH
6.75
6.03
5.70
5.50
5.35
5.23
5.12
5.02
4.93
4.84
4.75
4.66
4.57
4.48
4.38
4.27
4.15
4.00
3.80
3.47
2.75

CH3COOH
acetic acid

A
log

pH pKa

HA
pH

pKa

insert for pKa

0
4.75
0.5
4.75
0.5
0

<pKa+1pH
0
1

5.75
5.75

>pKa-1pH
0
1

3.75
3.75

8.00
7.50
7.00
6.50
6.00
5.50
5.00
4.50
4.00
3.50
3.00
2.50
2.00
1.50
1.00
0.50
0.00
0.0

0.1

0.2

0.2

0.3

0.4

0.5

0.6

Fraction Undissociated Acid

0.7

0.8

0.9

1.0

60
70

66
60
70

0
66
66

120.0
92.2
95.8
94.4
94.4
94.4
0

92.2
95.8
94.36

100.0
80.0

% HbO2

pO2 mmHg % HbO2

5
5.2
10
9.3
20
29.9
30
52.9
40
71.5
50
83.4
60
92.2
70
95.8
80
97.5
90
98.9
100
99.1
110
99.5
120
99.9

60.0
40.0

218
10
20
16.1

10
20
16

0
16
16

21.8
9.3
29.9

9.3
29.9

20.0
0.0

0
21.8
21.8
0

20

40

60
pO2 mm Hg

80

100

120

week1
week2
week3
week4
week5
week6
week7
week8
week9
week10
week11
week12
week13
week14
week15
week16

82.1
46.8
69.7
60.8
46.4
87.3
51.7
54.6
68.6
51.0
86.0
52.8
77.0
93.9
94.4
75.0

Start at

100.0
90.0
80.0
70.0
60.0
50.0
40.0
30.0
20.0
10.0
0.0
week1 week2 week3 week4 week5 week6 week7 week8 week9 week10 week11

week11 week12 week13 week14 week15 week16