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Ipratropium Bromide

Molecular formula: C20H30BrNO3 Molecular weight: 412.4 CAS Registry No.: 22254-24-6, 66985-17-9 (monohydrate)

SAMPLE Matrix: formulations Sample preparation: Dilute with water to a concentration of 125 (xg/mL, add a 30b JULL aliquot to 250 |JLL 2 mg/mL mepivacaine hydrochloride in water, vortex, inject a 10 JULL aliquot. HPLCVARIABLES Column: 100 X 8 4 jutm NovaPak C18 radial compression Mobile phase: Gradient. A was 2.5 mM PIC B-8 Low UV (Waters) in THF: water 40:60. B was water. C was MeOH:water 50:50. A:B:C 50:50:0 for 7.7 min, to 60:15:25 over 5.3 min, re-equilibrate at initial conditions for 5 min. Flow rate: 2 Injection volume: 10 Detector: UV 220 CHROMATOGRAM Retention time: 5.9 Internal standard: mepivacaine (8.2) OTHER SUBSTANCES Simultaneous: albuterol, fenoterol, terbutaline KEYWORDS nebulizer solutions; stability-indicating REFERENCE
Jacobson, G.A.; Peterson, G.M. High-performance liquid chromatographic assay for the simultaneous determination of ipratropium bromide, fenoterol, salbutamol and terbutaline in nebulizer solution. J.Pharm.Biomed.AnaL, 1994, 12, 825-832

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