Change in Mass (g) of Simulated Cells of Various Solute Concentrations in Distilled Water Concentrations

for 20 Minutes
Uncertainties: Mass +/-0.005
Time +/- 0.005

% Change = Final-Initial X100
Initial

Sample Calculation Trail 1 0.0 Concentration 5.28-5.05=0.23
0.23/5.05x100=4.6%
Average % Change= sum of all samples
Number of samples
Sample Calculation Trial 1 0.0 concentration 4.6x1.2x0.7x9.7x-0.6/5= 3.12


The Percent Change Mass between Initial and Final Mass of Simulated Cell



Initial and Final Mass of Cells (g)
Solute Conc (M) Trail 1 Trail 2 Trail 3 Trail 4 Trail 5
0.0 5.05
5.28
4.92
4.98
5.37
5.41
4.96
5.44
5.44
5.41
0.2 5.44
5.48
3.50
3.82
5.38
5.30
5.04
4.76
3.79
3.77
0.4 4.62
4.93
5.50
6.35
5.34
5.84
4.90
5.52
5.19
5.69
0.6 6.15
6.57
6.02
6.52
6.11
6.85
5.31
6.09
4.15
3.39
0.8 5.24
5.77
5.54
6.41
6.14
6.98
5.08
5.84
4.31
5.13
1.0 6.84
6.93
8.08
9.36
5.85
6.78
5.60
6.22
4.82
5.88
% change in mass
Solute
conc(M)
Trial 1` Trial 2 Trial 3 Trial4 Trial 5 Avg. %
change
Standard
deviation
0.0 4.6 1.2 0.7 9.7 -0.6 3.12 3.71
0.2 0.7 9.1 1.5 -5.6 -0.5 1.04 3.29
0.4 6.7 18.7 9.4 12.7 9.6 11.4 4.59
0.6 6.8 8.3 12.1 14.7 -18.3 10.45 3.11
0.8 10.1 15.7 13.7 15.0 19.3 14.76 5.29
1.0 1.3 15.8 15.9 11.1 20.5 12.92 7.30















-10
-5
0
5
10
15
20
25
0 0.2 0.4 0.6 0.8 1
%

c
h
a
n
g
e

solute concentraion (M)
Change in Cell size
Series 1
Maddie Wallace
10-30-13
Block 1-4
Osmosis lab write up
Research question: How does concentration of solute affect osmosis?
Hypothesis: If the solute concentration is increased by increments of 0.2M, then the solution will not
pass through the cell as easily, because the increased amounts of solute cannot pass through the cell as
easy as plain water.
Variables:
Independent variable: solute concentration (0.0, 0.2, 0.4, 0.6, 0.8, 1.0)
Dependent variable: change in mass (grams) as measured by an initial vs. final mass on an
electronic balance
Control variable: Distilled water, time, amount of solute, temperature of distilled water, scale used for
measuring mass, type of artificial cell tubing,
Materials:
600ml Distilled water
10 small pieces of string
5 200ml beakers
Artificial cell tubing (5-5cm strips)
Granulated sugar (solute)
Electronic scale
Timer
Food coloring
Separate beaker to wash tubing in
Paper towels
Funnel
Graduated cylinder

Procedure:
1. Cut five 5 cm strips of artificial cell tubing
2. Run the tubing under water for approximately 2 minutes to open it up
3. Mix the 5 different solute concentrations(0.2,0.4,0.6,0.8,1.0) with about 5ml of distilled water
and stir until the solute is dissolved
4. Color each solute concentration a different color for labeling purposes
5. Tie one end of each tube closed with one of the ten small pieces of string
6. Fill each of the five tubes carefully with a funnel, each tube having one of the solute
concentrations
7. Tie the opposite end of the tube leaving extra space inside the tubing
8. Rinse the filled and tied tubes in a separate container containing approximately 50ml of distilled
water
9. Gently pat the tubes dry with the paper towel
10. Take the initial mass of each of the five tubes using an electronic scale. Record the data
11. Place each of the tubes in a separate beaker each containing 100ml of distilled water
12. Let the tubes soak in the distilled water for 20 minutes
13. Take the tubes out and gently dry them with a paper towel
14. Take the final mass of each of the tubes using the electronic scale. Record the data
15. Repeat steps 1-14 four more times
16. Using the data from all five trials, find the average percent change for each of the solute
concentrations

Conclusion:
In this experiment, I created artificial cells to try to model the process of osmosis within the cell.
The tubing represented the cell membrane that the solution had to go through to get into the cell.
Based on my data, I learned that the average percent changes were 3.12 for a 0.0 solute concentration,
a 1.04 for a 0.2 solute concentration, an 11.04 for a 0.4 concentration, a 10.45 for a 0.6 solute
concentration, a 14.76 for a 0.8 solute concentration, and a 12.92 for a 1.0 solute concentration. These
average percent changes tell the average amount of water, throughout all the trials of each solute
concentration (0.0, 0.2, 0.4, 0.6, 0.8, 1.0), that went into the cell. This would give us an idea of how
much water goes in and out of the cell during osmosis for each solute concentration and if the solute
concentration had an effect on the osmosis process. In my experiment there were a couple of mistakes
that can be fixed for next time. One of the flaws was that the cell with the concentration of 0.2 was not
tied tight enough and therefore lost liquid. This affected the end results and therefore our end percent
changes. Next time I perform this experiment I will make sure that the tubes do not leak throughout the
osmosis process by tying the strings even tighter. Next time I performed this experiment I would make
sure to include more solute concentrations to get a more varied analysis of how much the solute affects
how much goes into the cell.