DENTAL BIOFILM

NATURE
Soft, adherent,
structural deposits

are
Mouth or
wet
environment
Dentition and
removable
appliances
Are
found
in
includes
microorganisms Intercellular
martix
Extracellular
polysaccharide
Composed of
Protection to
species
Nutrient
uptake
facilitation
communication
Bacterial
blooms
Palisading
appearance
in electron
microscope
Signalling
mollecules
Genetic
information
exchange
How?

Composed of
Organic
constituents
Inorganic
constituents
Function
Maintain
integrity
Prevent
desiccation
Prevent
attack by
harmful
agents
Acts as buffer; assist in
retention of
extracellular enzymes

Pellicle formation
Bacterial adhesion
Sequential
adsorption
Detachment

formation
Characterized
by
Doubling
times are
rapid
initally
Contain areas
of high and low
bacterial
biomass
interlaced with
aqueous
channel

Dental Biofilm Formation
Pellicle formation
Conditions biofilm
formation
Pellicle forms 30 minutes
after brushing
serves as protective
barrier against desiccation
changes tooth energy
Bacterial Adhesion
facultative gram + cocci
streptococcus sp.
actinomyces sp.
bacteria with protein
adhesins
multiplication of adhering
bacteria
coaggregation
growth of ECM
Sequential Adsorption
shift to an increase in
gram - anaerobic bacteria
virulence factors
trigger inflammation
Detachment
migration/translocation
oxygen gradient