Datos

tiempo [min]
0
15
30
45
60
75
110
125
140
155
170
185
200
215
230
245
260

Biomasa (abs) Glucosa (abs) Glucosa (g/L) Biomasa

0.340
1.551
6.517
0.280
1.689
7.097
0.214
1.743
7.324
0.240
1.654
6.950
0.336
1.533
6.441
0.230
1.730
7.269
0.2
1.721
7.231
0.216
1.679
7.055
0.244
1.433
6.021
0.22
1.525
6.408
0.239
1.567
6.584
0.263
1.7
7.143
0.268
1.561
6.559
0.292
1.627
6.836
0.314
1.634
6.866
0.316
1.69
7.101
0.325
1.366
5.739

Yx/s
mu

(g/L) LN (X)
0.9770 -0.023
0.8029 -0.220
0.6113 -0.492
0.6868 -0.376
0.9654 -0.035
0.6577 -0.419
0.5707 -0.561
0.6171 -0.483
0.6984 -0.359
0.6287 -0.464
0.6839 -0.380
0.7535 -0.283
0.7680 -0.264
0.8377 -0.177
0.9016 -0.104
0.9074 -0.097
0.9335 -0.069

0.1193
0.0038
0.000

50

100

150

200

250

300
7.400

-0.100
-0.200

Ln(X)

7.200

f(x) = 0.0037844426x - 0.995849406

R = 0.9489239523

7.000
Evol. Biomasa

-0.300

6.800

-0.400

Crecimiento
exponencial
Conc Glucosa
(g/L) 6.600
6.400
Linear (Crecimiento

-0.500

6.000

exponencial)

6.200
5.800

-0.600

Tiempo (min)

Estndar Glucosa (abs)

1.366
1.433
1.525
1.533
1.551
1.561
1.567
1.627
0.238
1.634
1.654
1.679
1.689
1.69
1.7
1.721
1.730
1.743

P
1 0.0294
2 0.0882
3 0.1471
4 0.2059
1.20
5 0.2647
6 0.3235
1.00
7 0.3824
8 0.4412
0.80
9 0.5000
10 0.5588
0.60
11 0.6176
0.40
12 0.6765
13 0.7353
0.20
14 0.7941
15 0.8529
0.00
1.35
161.3
0.9118
17 0.9706

GPN glucosa

1.4

1.45

1.5

1.55

1.4

7.400

f(x) =
R = 0

1.2

7.200

7.000
6.800

Absorbancia a 600 nm
Evol. Glucosa

6.400

0.8
0.6
0.4

6.200

0.2

6.000
5.800

1.6

1.6

Evol. Glucosa

xponencial
ucosa
(g/L) 6.600

miento

0
0

50

100

150

tiempo (min)

200

250

300

Pes

GPN glucosa

1.45

1.5

1.55

bancia a 600 nm

1.6

1.65

1.7

1.75

1.8

1.6
1.4
f(x) = 344.5080536519x + 0.0033944302
R = 0.9995185898

1.2
1
0.8
0.6

Linear ()

0.4
0.2
0
0

0.002

0.004

Peso Seco [g/mL]

0.006

t[min]
0.0
20.0
40.0
60.0
80.0
100.0
120.0
140.0
170.0
190.0
210.0
230.0
250.0
270.0
290.0
310.0
330.0
Yx/s
mu

Biomasa
0.6020
0.5230
0.5130
0.5210
0.5270
0.5760
0.5950
0.6110
0.7160
0.7510
0.7870
0.8310
0.9100
1.0360
1.0540
1.2360
1.2720

Glucosa
1.7100
2.0520
1.8710
1.9330
1.3600
1.7830
1.8080
1.7670
1.7810
1.7330
1.7430
1.7140
1.7910
1.7300
1.6980
1.6630
-

Estndar Glucosa (g/L)

6.3806
7.6567
6.9813
7.2127
5.0746
6.6530
6.7463
6.5933
0.2680
6.6455
6.4664
6.5037
6.3955
6.6828
6.4552
6.3358
6.2052
#VALUE!

10.494
0.0036

Chart Title
1.4000
1.2000
1.0000

f(x) = 0.003574562x + 0.1019027615

R = 0.9785743813

Evolucin

0.8000

Axis Title

Crecimien

0.6000

Linear (C

0.4000
0.2000
0.0000
0.0

100.0

200.0

Axis Title

300.0

400.0

Glucosa
1.3600
1.6630
1.6980
1.7100
1.7140
1.7300
1.7330
1.7430
1.7670
1.7810
1.7830
1.7910
1.8080
1.8710
1.9330
2.0520

Biomasa (g/L)
Ln (X)
1.7375 0.5525
1.5082 0.4109
1.4792 0.3915
1.5024 0.4071
1.5198 0.4186
1.6621 0.5081
1.7172 0.5407
1.7637 0.5674
2.0684 0.7268
2.1700 0.7747
2.2745 0.8218
2.4023 0.8764
2.6316 0.9676
2.9973 1.0977
3.0495 1.1150
3.5778 1.2748
3.6823 1.3035

i
1.0000
2.0000
3.0000
4.0000
5.0000
6.0000
7.0000
8.0000
9.0000
10.0000
11.0000
12.0000
13.0000
14.0000
15.0000
16.0000

Title

Consumo Glucosa
6.80
6.70

2x + 0.1019027615
3

0.0

6.60
Evolucin de biomasa
Crecimiento
Linear (Crecimiento)

6.50
6.40
6.30
6.20

400.0

6.10
6.00
5.90
0.0

50.0

100.0

150.0

200.0

00.0

P
0.0313
0.0938
0.1563
0.2188
0.2813
0.3438
0.4063
0.4688
0.5313
0.5938
0.6563
0.7188
0.7813
0.8438
0.9063
0.9688

Chart Title
1.2000
1.0000
0.8000
0.6000
0.4000
0.2000
0.0000
1.3000

1.4000

1.5000

Consumo Glucosa

150.0

200.0

250.0

300.0

350.0

1.6000

1.7000

1.8000

1.9000

2.0000

2.1000

000

2.0000

2.1000

t
0.00
20.00
40.00
60.00
80.00
100.00
120.00
140.00
160.00
180.00
200.00
220.00
240.00
260.00
280.00
400.00
420.00
440.00
460.00
480.00
500.00
520.00
540.00
560.00

abs biomasa (600 nm)

0.5550
0.5570
0.5570
0.6500
0.5970
0.6880
0.7190
0.7690
0.9250
0.9830
1.0390
1.0980
1.1830
1.2280
1.2550
1.6540
1.6220
1.5940
1.6770
1.5840
1.8000
1.7860
1.8080
1.7720

Yx/s
mu

abs glucosat (505 nm)

0.7250
0.7430
0.8080
0.7660
0.7040
0.6760
0.6660
0.5690
1.2340
1.6610
1.7920
1.5920
1.2980
1.1420
0.9850
0.2640
0.2240
0.1390
0.0660
0.0000
0.0000
0.0000
0.0000
0.0000

estndar

0.2720

0.2900

Glucosa (g/L)
2.6654
2.7316
2.9706
2.8162
2.5882
2.4853
2.4485
2.0919
4.5368
6.1066
6.5882
5.8529
4.7721
4.1985
3.6213
0.9706
0.8235
0.5110
0.2426
0.0000
0.0000
0.0000
0.0000
0.0000

1.1206
0.0032
1.800
1.600
1.400

f(x) = 0.0
R = 0.94

1.200

Axis Title

1.000
0.800
0.600
0.400
0.200
0.000
0.0

100.0

200

Biomasa (g/L)
1.6011
1.6069
1.6069
1.8769
1.7230
1.9872
2.0772
2.2223
2.6751
2.8435
3.0060
3.1773
3.4240
3.5546
3.6330
4.7912
4.6983
4.6170
4.8579
4.5880
5.2149
5.1743
5.2382
5.1337

LN(X)
0.4707
0.4743
0.4743
0.6296
0.5441
0.6867
0.7310
0.7985
0.9840
1.0450
1.1006
1.1560
1.2308
1.2682
1.2901
1.5668
1.5472
1.5297
1.5806
1.5234
1.6515
1.6437
1.6560
1.6358

abs glucosat (505i nm)

0.0000
1.0000
0.0000
2.0000
0.0000
3.0000
0.0000
4.0000
0.0000
5.0000
0.0660
6.0000
0.1390
7.0000
0.2240
8.0000
0.2640
9.0000
0.5690
10.0000
0.6660
11.0000
0.6760
12.0000
0.7040
13.0000
0.7250
14.0000
0.7430
15.0000
0.7660
16.0000
0.8080
17.0000
0.9850
18.0000
1.1420
19.0000
1.2340
20.0000
1.2980
21.0000
1.5920
22.0000
1.6610
23.0000
1.7920
24.0000

Chart Title
1.800
1.600
1.400

f(x) = 0.0032259318x + 0.3881914192

R = 0.944964649

1.200

Evol Biomasa

1.000

Lineal

0.800

Linear (Lineal)

0.600
0.400
0.200
0.000
0.0

100.0

200.0

300.0

Axis Title

400.0

500.0

600.0

P
0.0208
0.0625
0.1042
0.1458
0.1875
0.2292
0.2708
0.3125
0.3542
0.3958
0.4375
0.4792
0.5208
0.5625
0.6042
0.6458
0.6875
0.7292
0.7708
0.8125
0.8542
0.8958
0.9375
0.9792

GPN
1.2000
1.0000
0.8000
0.6000
0.4000
0.2000

0.0000
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000 1.4000 1.6000 1.8000 2.00

00 1.4000 1.6000 1.8000 2.0000

t [min]
0.00
20.00
40.00
60.00
80.00
100.00
120.00
140.00
160.00
180.00
200.00
220.00
240.00
260.00
280.00
300.00
320.00
340.00
360.00
380.00
400.00
420.00
440.00
460.00
480.00

Biomasa
0.675
0.615
0.647
0.624
0.654
0.707
0.776
0.838
1.056
1.114
1.21
1.308
1.366
1.366
1.479
1.512
1.577
1.578
1.58
1.515
1.53
1.38
1.482
1.634
1.629

Glucosa
0.443
0.44
0.42
0.39
0.387
0.38
0.357
0.266
0.461
0.334
0.391
0.296
0.282
0.273
0.167
0.4
0.198
0
0
0.013
0.029
0.565
0.79
0.782
0.803

Estndar

Estndar

0.246

0.389

0.392

0.362

Glucosa (g/L) Biomasa

1.801
1.789
1.707
1.585
1.573
1.545
1.451
1.081
1.874
1.358
1.589
1.203
1.146
1.110
0.679
1.626
0.805
0.000
0.000
0.053
0.118
2.297
3.211
3.179
3.264

(g/L)
1.9494
1.7753
1.8682
1.8014
1.8885
2.0423
2.2426
2.4226
3.0554
3.2237
3.5024
3.7868
3.9552
3.9552
4.2832
4.3790
4.5676
4.5705
4.5764
4.3877
4.4312
3.9958
4.2919
4.7331
4.7186

LN(X)
0.6675
0.5740
0.6250
0.5886
0.6358
0.7141
0.8076
0.8848
1.1169
1.1705
1.2534
1.3315
1.3750
1.3750
1.4547
1.4768
1.5190
1.5196
1.5209
1.4788
1.4887
1.3852
1.4567
1.5546
1.5515

0.377
Yx/s
mu

2.629
0.0041

1.6000
1.4000

f(x) = 0.0040659327x
R = 0.9565033596

1.2000
1.0000

Axis Title

0.8000
0.6000
0.4000
0.2000
0.0000
0.00

100.00

200.00

Axis Title

300

Glucosa i
0.000
0.000
0.013
0.029
0.167
0.198
0.266
0.273
0.282
0.296
0.334
0.357
0.380
0.387
0.390
0.391
0.400
0.420
0.440
0.443
0.461
0.565
0.782
0.790
0.803

f(x) = 0.0040659327x + 0.3592744422

R = 0.9565033596

Evol. Biomasa
Crecimiento
Linear (Crecimiento)

100.00

200.00

Axis Title

300.00

400.00

P
1.000
2.000
3.000
4.000
5.000
6.000
7.000
8.000
9.000
10.000
11.000
12.000
13.000
14.000
15.000
16.000
17.000
18.000
19.000
20.000
21.000
22.000
23.000
24.000
25.000

0.020
0.060
0.100
0.140
0.180
0.220
0.260
0.300
0.340
0.380
0.420
0.460
0.500
0.540
0.580
0.620
0.660
0.700
0.740
0.780
0.820
0.860
0.900
0.940
0.980

1.200
1.000
0.800
0.600
0.400
0.200
0.000
0.000

0.100

GPN
1.200
1.000
0.800
0.600
0.400
0.200
0.000
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

S inicial
mu
Yx/s
1/mu
1/s
6.517 0.00380
0.1193 263.158
6.381 0.00360 10.4936 277.778
2.665 0.00320
1.1206 312.500
1.801 0.00410
1.4396 243.902

s/mu
mu/S
0.153 1714.949 0.000583
0.157 1772.388 0.000564
0.375 832.9504 0.001201
0.555 439.2227 0.002277

Metodo Lineweaver-Burk
350

2000

300
250
200

Axis Title

1500

f(x) = - 33.4082317396x + 284.696522659

R = 0.0498023569
Metodo Lineweaver-Burk
Linear (Metodo LineweaverBurk)

150

Axis Title

100

1000
500

50
0
0.10

0.20

0.30

0.40

0.50

0
1.0

0.60

Axis Title

Eadie-Hofstee
0.00450
0.00400
f(x) = 0.220982466x + 0.0034195101
R = 0.2212042811

0.00350
0.00300

Axis Title

0.00250

Eadie-Hofstee

0.00200

Linear (Eadie-Hofstee)

0.00150
0.00100
0.00050
0.00000

0.001

Axis Title

0.002

0.003

Langmuir
2000
1500

Axis Title

ee

ie-Hofstee)

f(x) = 266.3523409435x + 33.664828721

R = 0.9862324342
Langmuir

1000

Linear (Langmuir)

500
0
1.00

2.00

3.00

4.00

Axis Title

5.00

6.00

7.00

S inicial
mu
Yx/s
1/mu
1/s
10.000 0.00380
0.0560 263.158
7.500 0.00360 10.4936 277.778
5.000 0.00320
1.3359 312.500
2.500 0.00410
1.4396 243.902

s/mu
mu/S
0.100 2631.579 0.00038
0.133 2083.333 0.00048
0.200
1562.5 0.00064
0.400 609.7561 0.00164

Lineweaver-burk
350.000
300.000
f(x) = - 95.9686975412x + 294.3280065392
R = 0.1980062734

250.000
200.000

Axis Title

Lineweaver-burk

150.000

Linear (Lineweaver-burk)

100.000
50.000
0.000
0.000 0.100 0.200 0.300 0.400 0.500

Axis Title

eadie

Axis Title

0.00450
0.00400
0.00350
0.00300
0.00250
0.00200
0.00150
0.00100
0.00050
0.00000

f(x) = 0.4008522446x + 0.003360331

R = 0.379285925
eadie
Linear (eadie)

0.0005

0.001

Axis Title

0.0015

0.002

Langmuir
3000
2500
2000

er-burk

neweaver-burk)

Axis Title

f(x) = 263.4520753102x + 75.2166238768

R = 0.9768271142
Langmuir

1500

Linear (Langmuir)

1000
500
0
2.000

4.000

6.000

8.000 10.000 12.000

Axis Title

eadie
Linear (eadie)

muir

ar (Langmuir)