NI DUNG

II

Gii thiu v curcumin

Cc phng php trch ly curcuminoid

III Phng php phn lp curcumin t curcuminoid

Gii thiu v curcumin

1) Gii thiu chung:

Cng thc cu
to

o Thnh phn chnh ca curcuminoid mt

cht trong c ngh
o Curcuminoid gm curcumin (diferuloylmethane),
demethoxycurcumin, v bisdemethoxycurcumin.
o Tn ha hc ca n l(1E,6E)-1,7bis(4-hydroxy-3-methoxyphenyl)1,6-heptadien-3,5-dion.

CTPT: C21H22O6

Dng enol

Dng keto

Gii thiu v curcumin

1) Gii thiu chung:

Tnh cht vt l:
o C mu vng
o Tan trong du, ethanol, methanol, dichloromethane,
acetone
o Hu nh khng tan trong nc mi trng acid v
trung tnh, tan trong mi trng kim.

Gii thiu v curcumin

2) Vai tr v ng dng curcumin:

Ti sao phi
tch v tinh
ch Curcumin

Gii thiu v curcumin

2) Vai tr v ng dng ca curcumin

Vai tr ca Curcumin:

Sc khe
Sc p

Gii thiu v curcumin

2) Vai tr v ng dng curcumin

D dy,
gan,
tim
mch

Tr hen
suyn,
ho,
cm..

Khng
vim

Phng
chng
ung th

Tiu
ha tt

Phng
chng
HIV/AID
S

p da

II Cc phng php trch curcumin

Nguyn tc trch ly

Cc phng php trch ly trc y

Phng php trch ly bng siu m

II Cc phng php trch curcumin

Nguyn tc trch ly

II Cc phng php trch curcumin

Cc phng php trch ly trc y

PP trch ly

C in

Chng ct
li cun
bng hi
nc

Dng dung
mi hu c

Hin i

Dng
soxhlet

S dng
CO2 siu
ti hn

Chng ct li cun hi
nc
`

Nc
nng
Hi nc

`
`

Chng
ct li
cun
X l hi
nc

Lng,
gn

Sy

Tinh du ngh

Curcumin

PHNG PHP CHNG

CT LI CUN BNG
HI NC

C ngh

X l
Hi nc
Chng ct li
cun hi nc

Tinh du

Cn rn
Nc
nng
Lng,
gn
Cn,
rn
Sy

Curcumino
id

Trch ly bng dung mi

hu c
Ethanol

Este du
ha

Trch
ly

Sy

Trch ly

X l
Tinh du ngh

Curcumin

C ngh

X l
Ether du
ha
Trch ly

Tinh du

Cn rn
Ethanol
Trch ly

Dung dch
lng
Curcumino
id

B rn

Trch ly bng soxhlet

Ethanol

Este du
ha

Trch
ly

Sy

Trch ly

X l
Tinh du ngh

Curcumin

Trch ly bng CO2 siu

ti hn

Nhc im ca cc phng php:

Tn
thi gian

Hm lng
Thp

C in

Curcumin d
B phn hy

tinh khit
thp

Chi ph
cao

Thit b
phc tp

Trch ly vi s h tr sng
siu m
Trch ly
bng siu
m
Tinh
du

Trch ly
vi
nhexane

C c

Curcumin
Kt
tinh

III Phng php phn lp curcumin t curcuminoid

Nguyn liu: bt curcuminoid c chit

sut t ngh vng
Quy trnh phn lp:

Bt
curcuminoid

Kt tinh li

< 95 %

Tinh th

Sc k
ct
Curcumin

Curcumin
tinh

H dung mi methanol
nc
Thi gian: 24h, nhit : 5 o
C

Silicagel 60G 63-200

Dung mi: 100% CH2Cl2

III Phng php phn lp curcumin t curcuminoid

1. Kt tinh li:
Loi b tp cht
Mc ch:
Thu phn tinh th c hm lng Cur cao
Nguyn tc: Da trn tnh tan khc nhau ca cc cht
trong dung mi theo nhit v tinh khit ca hn
hp em kt tinh
Dung mi s dng: Methanol, nc ct

III Phng php phn lp curcumin t curcuminoid

2. Sc k bn mng (TLC):
Xc nh h dung mi cho sc k ct
Mc ch
Theo di qu trnh sc k ct

III Phng php phn lp curcumin t curcuminoid

2. Sc k bn mng (TLC):
Nguyn tc: Da trn s tng tc khc nhau
gia cc thnh phn trong hn hp vi dung mi
v pha tnh
Nguyn liu:

Bt curcuminoid sau 3 ln kt tinh

Methanol
Dicloromethane
Cloroform
Acetone
Bn mng silica gel 60G, Merck

III Phng php phn lp curcumin t curcuminoid

2. Sc k bn mng (TLC):
Pht hin vt v tnh tr s Rf:
o Hin hnh cc vt sau gii ly

Mu sc t
nhin

Phun thuc th
trong dung mi

n UV

III Phng php phn lp curcumin t curcuminoid

2. Sc k bn mng (TLC):
Pht hin vt v tnh tr s Rf:
Tnh Rf :

Nhn xt v nh gi cc vt trn mng mng

chn dung mi chy bn mng ph hp

Chn dung mi ra gii ct sc k

III Phng php phn lp curcumin t curcuminoid

2. Sc k bn mng (TLC):
Cc my sc k bn mng:

III Phng php phn lp curcumin t curcuminoid

3.

Sc k ct:

Mc ch: Tch, tinh ch Curcumin t curcuminoid

Nguyn tc: Da vo i lc khc nhau ca cc cht trong
hn hp ban u i vi pha tnh trong ct v pha ng
qua ct
Phng php thc hin:

o Ct thy tinh hnh tr

o Pha tnh: Silica gel 60G, 0.063-0.200 mm
o Pha ng: Kho st trc bng TLC v
chn dung mi 100% CH2Cl2
o Mu: bt Curcuminoid kh

III Phng php phn lp curcumin t curcuminoid

3.

Sc k ct:

Tin hnh

1. Chun b
2. Nhi ct
3. Chun b mu v cho mu vo ct
sc k
4. Trin khai ct
5. Thu v kim tra thnh phn mi
phn on bng sc k bn mng

III Phng php phn lp curcumin t curcuminoid

3.

Sc k ct:
My sc k ct: