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Stones ies ator tanec Ho alrose mora ws rvow at Is en suey ko pave Lambert mcr tl meas veanene pc pz noisy ech asorbuacy arnt Sgt SII (pa a, gy grow ea Ses ae Pree cn ms Ftaemey ome te he 30) Ponce ras pat one er se ie 1% pire ose ice so 0% perwotne o> ante epic poclons 2" ‘ont od dlvgosi fal swiats, temperstry + repuscaings W sah | omacza sig jako wapoteryemikabserph ik) Rowonnie 7) payer wlady oat loleeymcii cnsyweny sche — ryema OD} Prawe Lanter not Pramo Lambert sls: absorbant iia menccramatyemes » foe reporceane do ral want) wee in, Pn siete inant na coat can a gor Lana mena one ier at Eee pas mr ee SE eu one apace some Boe dset (toi ono: Abort baineym jst proporcionan 0 eapore tem, shebanc Snit Se lok AY 1 ces etehy’t hb A> —heT ramming mi of 0401 od do 10% Mepeeerneme setoncene wis ale prays ‘Setar rss 2 Stee fast) sm eas an prin mene» sie 0 silage et zm pay amin absorb ocean tae Saran ere OS warsny rotwore, ns ‘Ssoapmpowad treat a puboc wast, Kors ures San et bt mah aah ‘ote et Wide shor aj stan har) ms A flyaion wrt ly in dps fas (2) isn Geese sae ee 9 guboss warty 1c “Diao vriors Soloyacsoning fea remetowsnes ea Wye anatycrnl dugot ak Prespszani par sotwe Svat cai ‘ina, Kora jest makemalac sbrotbowata paw e sbervowane i tetrycensch nay unt pat monochromt)ezgo(poburwmeg) of rekon) Slugs fa Swit monochromatcne unskane w spektloo fetrach 2a pomeca pryematow lib ssh dayne, smativia pawl micztanic renew taeei ary, amiss | dokiook pom awa pewewens SSeS aiarpa w HSH os C1 i gestae oP My YG “en 7 006ZVTANE ABSORBANCST og wus rotor ested ra er 4 ide) MOWAC, do Hoi reese a rere 0° HE song «rie ae bit satan pric baane) ch w pile wroreowe}) ub tang bie badang) = dpi) Bob io aang w probe wzorcowe) cm wr oy rs wai sanych notch weit ‘hla real ea ge ‘rele thy pate tacos» tlh samychdnostach probe Badan, np. w mg, wg, umcl itp, . MND tld far a fof seni 15 p/n, dada minal eves ekonoge spinon Fry ble 0 ae nhs eno warok aborbaas Mens te ini tng pce) Sa SGI s sady Say nt? PO WY WOlanin zaber smg/100 m= (yA ing) mt) = 030, {9 Poniewaz Kolorymetiomany rztr lege 4otm ‘erie absorbunsh towne! mig hes oe cht HEP = (4/4) u8P w pric waorsowe) = 0150011) 10 = mg/100 m= (4/4, og100 mt} = ,309,812)15 = 20 4x wide 2 powyiyeh ote, sce fra W mg bd sie zmienilo sig po. M4rotnym roche Islrymeronanegn estore, Ob {eto Kolorymetrowanego rouwors ne wplyna wis maceana wate Seta Poniewad sieiesubsianoblica sg nie w baway holorymetowanym fa Sworze(czl nie Sub 18m lew badane) poet, —w aay pea ‘2 il roby brane} do ywolnia bares (opie mowite © aaizowang ‘ance, surowiey, kr tp) Ite est natomiset Sey bosses ote ba ‘mg Kelrymeicowanep> roiworu bla zawsw jdakows © robe bala Usa: Rozciesczeni Kolorymetromanpo rortoru wpa ene as cane) sbstangji tylko wed, gy jet to sata barwna (op ore ck ‘hemoglobin Tob edly dane soba ma wiicwos sore rominiovania ‘flee ey tedy bepotedio ery absorbance rato HEC “eucen sp stone dan} substan, ber upoednies wywolywana aw (ate peaked 3) Woptezyenh haltech Sigel sutstansw pie badang| bir = wa = 3258 Le prance foztvora wzorcowego w+ a ‘uma naome oa nan Maro suaku absorbs. sposobami ‘ie badane} przybiera nas a ar ot = OH 08) 4 W metodach 2 zastoswniem nturanego lb scmeyo ware arn, 21 ‘hm mi wykonoje idee procetury nywoywana bury «Meg tong tlcsytje a Bezosrsn w petrokolret no obi asp _-omsiehraiebaanego materia w casi proeedry Sieéenieweorca ale w tym wypak war takch samych notch — jk igen aban w pre had) ap 8% m1, 10 mi aati w pric plans) we edeosthach mas ere len Meme es es ‘oink (8) é alances mac ee Sica emer 2 cw pi aa ~ Wt “eat son/2-= 2098 F100 1 Pe ee eet ty 0.) i = Peseta rome ra om PO ia Fee a ole 2 IN san eet ageae Few arowicy tei 1 eh nh eh este 10 10 oes i Tare alow sa = HOaLowy 0:2 Moye Fet0 a po in ye oj os phd sei Few 58 Sheen Geetacs las ee po Unb gn Fev ome ‘seen nis yb fs sie Fe alin pit cl # a suowiy) =p A,)4 vs Fe = (0800444 yg Fe UpiAttmaFe= 20040, 421g Fe ate atom ec Fw smi 0, A = C/A.) ig/100 mt) ~ Mido Searels frat ry mn linedivtprestepinerit alin D ei trios sonny aor a Few ng sini Few suri (100 mi) ~ 4 er aa nee niece emg ce ee in cme oan ge Si at oy nt ton sia mn oe (Raiaie'ga! Pobrano 4 a surowcy,dodano 4 ml rewor CC,COOH 12 al Fee csaais barry pobrano # ml pragacre ome 2, waters Fae. ow at ecg sicenn § mgfoL Onieyé mecinik ie ici Fee eyo maroc ng/00 mi] podang metoteean Sera Moewigpae: ley obeayé Hoi aroniny wana do lean bE G9 objeto srowiey anaes» 6 pss) 00/0 = 0 10pastes i ; wate meses ost et} Hl dowywotnss cm ines owe ee ei me substanci tad eae ian = me 8 eno lL rr ssc ac Da rortmore oksphemozlh Se eeeioca” See gating we Ki omacza sit 2 hss urine W i etn se yoania zabarwicnia), a wi: ei cower ore ora Sek seeteeteron pink Kemet meseirees 30 27) (05 cl ioe Noa iio x0 - si we t= (44a = (031055) 24 aye tee roa Wl wpe rye pier sig re oi ‘ator wecnesveeogm sein nywaie wo zabormiense ok tae march mt yt are abort) py mybranc) de ‘SN ps ey nocac w ch mart abvorbancy. 2 9 sc cg ep ip) ub warts zc (954703 toa rem wy sew pie Daan). Ob om tery KEY mest me 10 rae aw dans bad ms yn ey ren Hay eo materi, bra) = tm an wc 50> c oi be Pele 36 Do 4m Riba, on] = 015-70 pl = 105 pe, rmnoiniky = 045 ml 70 pg/ml ~ 315 ye » sooth (om) = me/00 mi): Ojos surowiy (K,) we more niet bjt poeta (mong lee objeto brag espotredna do mywobna bre cy aja se» 3 4 srowiey — 10 ml otworn 4 +6) eal Sm) seperate 2 ml surowicy — 20 onto koe oboe w soe) xml — "$l rotworu 16 ml waworu 8 +3+5) 4 ml pasa 502. soy 08 ml ewowiey — alng - vean-aese05 0 = Sees vein 90 20.10 ot a seems nen n= ty fp 05 12m m= 656 4/10 ‘yt mma a oe (ie dear 2iti,poiewa» ch He snare nec rae} Bo wy bar apo ORS wk sacowicy (8 przesacry fez wars mnoinikow do olin cas empl 2 ia ated oben je trzes pode | ee ews. k wad, kx pare 025m ‘een mir sara 9m tpt wine mayaich pn Sea rah mona sorte inp rei a pres Lamberts ee do stghenin. Preyeczynai ‘maa (leone 16 Palermo barn o gen = Clernie22 Ws analiyom) gu Goeznt32- r aatymy g a ykeieealrcy Do 7 probiwek dodué Kolo 0 1,2 Zawirsht aayulich provrc wapee wou fe 5 al cole OL fexwop Evert llvlcotowegs onal 374 srera sacl 1. Wybir dag ak, Ozmacné w fotki tale pe yc go {ach (yw lve 40-800 wari sorb a otros ee yeh wren Wes hreywe jk ure 32a opal ge 2. Wyhrdlane bre laienye Pray wytarych pi ih macy aoe tance dln wept sworom wo by kong (nobowha Pike konto rr tcana nora odsosnier ab prob dee sal si = wx dyke fe wiawieiekomory at amare Oa carne, a * demtogas ulon) opis 4.2, CHROMATOGRAFIA ADSORPCYINA Ronda ebitanj © proce hr Sdeorpei oweriniowane she ye einige teria. Neu weet ay = 5s do powers ad WY okrclonyeh warvokach waa i Se era vores wy nytt Snes cores Sa weitae te rSetieg! rey echo brn Lin cma a rasterck pottzcbprzypamni) 20 i. . - TChrommtograiadsorpeyon ech sepier ety: 1) specail ‘a wige masivoie ronal sktadaikow zizonych pod wraedem shad che myo, nie dajpcyeh si roadietrwpkis mata kpc tb fk ‘Frawehne.dnigiagy 2) univer, nods wt 40 rendab ob- Teng bacco inych, od weglowodoré nepolaayc Ib Sabo olen do aad oreanszayeh 1 Kvasoe alle polaryeh | reaktymayhs 2 were mad Talc alan earaokw rozdzatachromaioptczneo, 4h ania sa rato adsorventty 4) exis np. ma ohunol colru.o iy mi HES Teiise 1100 ng coro ow oben yeh oii clnoin b i aden Mraeacee stone regan exes tomato adorns 40 kt panicae chomatogti ecorpeing, male sw Web mia i a bya 2 Yaowane headracnie PE Nic moéna jednak pomina ona aastorowanie ko do road ftrktery chemice} pola 2s AC tego. Ponadioskadnksadsorbtu ne mow AE advrbentem i eo wines we mea Bre aH borbent o__ samen teumey mow Man scrim maa ®TOINK aor — ey Ee enedie ire ga rxpuzanhin. W ay 5 ere Gra es on ‘evel ania erty sie polar (ap. SO, 10.1 ‘io ple lip. MEO, CACO silane op eg aktywowany pra, ae ek set. det pin owas nserse® RU a ree MIMS atmctsporedwhitmoicapesferosc des SSetearemeetpoml wong, ne rego £02 ey age ny inn mens sion. srhh an ae is min nsrbentck chor Sees hm amr roe of KONE ser, No ee ss amos a ; a acer cea answer ce ndan roresenks | 142 caym wpa pion adsorpcyne do dis wha Ulu chromatoraicen) aly od pw Peete epee deh suc w lle adsorpesinym, Czynmk ‘unsere arp vin sq 7 natu advoeots Somat att a aaa tae bor > sich. see > midovday ensscone > mln ‘ret sos cet tee srt wr wr 7 iia pods rai ith podsavniz tego samen 0222 ser ac at Awa stan» Dobie slameniw ide chromo ood mii) ke ‘Tabych adsorbentach czastecck adsorbatu prawie nt leet adsorp i calkowiowst ah © 9 sesh rt se reat tys ur Deena cs se cision (hon 2 tan ch Llane ose ips Won Bint 1 WN. Ne samy wel 2 hor arepy CHO. cans ary AO ws 0 er eat on. Wop ose sj Paro mca pe sf) maylue rastosowanie sins ‘keiowych, pier | masach exqutccskowsch erage eee aes ay a web rant, Nad me poiercni so as 2, Prrygotommiewyeage sie 2 hos po Gedicrrs Bout» ane 296 prez bul zona cern nallonse 4. Rozwijanie chromatogra, Praeicoay iy cay wycigg tones rnc cance fu skr0bi, dodat powol aly parca ni cen sane osuck| entero Ait ie wpronadsi na klum exten) wey) ead ; ck 15 ml erp domes mszaniny eters aftowcg, etna ders elomego Obrien roaddal barwnikow ls wedug naepuaes Llgoout oly chloral b (Podroede 11.7, ktbry nasi sores sho, pana wea Yotumay, pod sim — nebestoacns wanna lof at My atl ‘ajme polozene njiize 4.3. CHROMATOGRAFIA JONOWYMIENNA 4431. Charakterysiyka procesu wymiay oro CChromstogafiajooowymiena ples a wymini wzyskchnoych do wy ny jondw fdodatnich | jemnyeh) sorbent onl mt ine joay 0 tym samy Hada, aware w rortwore nity 1 ci sale nitozucre w woke med ins yeh ozpuszcalnkach rguncych, Kt aj whines wymiay jon 0 ‘ah estan nya en won. Se EN tad trdkoceyteerkowe, w lorych myodrebia sie mate w poste bes) sit resrznne) ora grpy chemizne cnn (rap anki Awan ob ass Wee, zawnae sajte pene joy oak peacinye. Rodzaj gop fenkynpeh sede o eharakteraeeaksi ymin:pepe ices coon! —OH>~su, vote sees SHC i See vw proves rene (ej en aie 7 rere asa ich sl ymin sore sing 2 Herc Malan eran abo sale teh Zt Ste noio TEEONLE MTN Ty one at oF =NH,011>—NHLOH SSE sangeet iad eect tan nny = papa aminowrms ereatornesonyey (Eom a eur she rn See emer aint sue weg schemata S17 so Penman Loa! Towarncre [ae Si” Toren = asinine jy O- emi “anionic jonas ruclingm. HS nym tyne nae (8 soos wymienna jonity jet 10 ilose 300% tiem ab boa joi. O22 mt ewomeniac Ceili. Perry ways Chita ego caps wymcnney Mealegineych wyimanic [np SOI Geempeh poocesto © wrmienian, Drain Capen oe enact en foal oztword celts lent aoe ae jon wymienny 0 wickszym poninowactne do sass nen oes ie enn w poe Fo emo cy ly spa Podutawy chromatogra jonowymenan et genie een ax jen 0 taki samy zaka rene 1 mute, ee = mies oe ls pinsch od ext polars plane joy rnleanyen ea retnora ease Pere O Bitinstarowene ri sano 1 Gna See toeae expres nse si Saepareormtce mri sy et et so oe ce Stace pe ji Ses a et eee le Te corr) nines en Set pgs aan ne avis + sre loon ‘ Taian saber HU Trym Zywie pityrenovs wih bes iy coma Poa a mjpemn 2 a et etalon) fabrz. Dy = eno ju usieciowania reedu 15-2) een tore ean apy op eC I es me ado foo 4 sae a ein ec ice a saa 2 Pas nan See nua tic ibe aby elem en # Pacers cman lee oes ta tn ee Serercmemnr onsen —— =r a Rename eon ; Ta aneaespiy nip rene (~COOM. Ok = 2= oemnenenaee ‘pl tatomian aalonity prupami —NH,OH zachowujy sig jak stabe sas) er ake ras \2etatjcaniie ee ene rain =e ah Seep oe ee gy nse Stare re oo ey spinon 56° iy ee 0047 peta pam — poe pliner ‘Te poo Aol ‘ian i ete ot mpvadii 2) ‘te driers Spe om ‘ibe funk — sce =r nrg Oe diwinyl. Siee preest sos one mo Ile 1V-ragdowe, Najsab ant inowspierwszr7¢403, 8 ay. Morales grupy czy gem perze mieSce SUN} ft Ueropowania polame st umes any, Belacym pochodn sirens. Dia mika ssilgceg. Najeeici st ry procentowa aarti div Mirmlouniny ayeny{dvinsloderzenu pred ee pate tems puirnoweo os erro recy amp eran pes ho — == 0 0 ae -hi—on, ght50 ees wage wane ol He til inte Ada nec (og Moi ren wesue 8) der one oem. 2', ali eh ah oath since sie = spongy tak. wit ney 0 rh tem xp yey hms pou > (Pela: 1% mt amino. Pe mia tay tas = even 42. atetss Arinokwasy w formic anor mone RroW takotene na 1 M NCH wedh 20 ml 1 M tomtworu HCL Iub ('M rectors ROOK ene dee 2 nM owe HC M tet WOM wt a Titanate ray woda do usa okzyeu shen hase rao > EANO, w presers w prspadku HC Po seta peemjeeody a Dspptowang zawisig jotu preci do holy emule DObaliyé Geez w Lolurmne sklae do prep! pocome font, Naa ortwora kate gtaminowegs, Prec ova mu tohneyspowedavad wa Iie roatworu bie yee orang kan 4 soda zbiraé po ok ‘emt npc do pobtwel wk On AgNO. Obsrwowaéstrqane joni Cl” mypajgachskounay {Grceenie 4. Cheomatrgrafiajnenyienmma CM cela ston gray bye Ieeg HL Nohara wep 168: Batch Boys. Act, ISKSIS-S39) “asad Do roel nsednortaych bak zsadowch toe nowy Kationowy, CMt-ellog gupams kwasowyzs ~OCH,COOH. Ns wrk ae fini przemtepobuforem ocatonym 0 pH 42 ma) le now) wpromada fewiel se stoma rozpusconep tm samy bfos: Cesc ions {jer po ston Kwesowe swojege rk ue KEEDEHD Wt w Psa atondw (NH,") i aosaitzoizane paz aiosomae annowe aura CM =e fary PCHCOO) 7 rea si alee od Bosra sep salon, Pm tiene Kun contworan orks sik enw) oii tte wrap dahon balk kaon 1 prema Tone aleaioy emply Jil wiadome, 0 Ry wpich rnd bong et eae SOM moian odie’ celord see prnnge nepwxcaata | 5m soap trelininowné wymyte presen rpc ancajecria by ) doy sunigin jogo dadow w roku samen, prcpuices 9 peat home eet eon crenata — arama nt pn ruins i tkemopaionstchowsogranspeeoncp = ya sme ts wo see oe Cuma ius repress penis yo Ma = Recnd se saprewian roc pc vet iy saan, Wink ‘Sn rm oe ep et nce mse rye mosis dang an» ews do poms Polgs ons ma poddawani ‘ing a mses eno an ne vich aye temo rozpuszcralnikow » 0: edn — rjgco-wstgpuac® aan ro romain hontgrine ca 5 oi 2 wsigpaaco- ster irene, Ou sae | cans ey | et re Dwscronce wins hie’ ceo mie mn en rc a pom ng | Saar mete ag tame ara pus, Coron Saag en ni rh ome ad ayn eddicnyn 6%" mp ps ey he ‘enon rein cr rte mnt. gy hea mance etme Se race is ——ieee se a secon cmovesss ow 10 Yo He sh ous Mone tad erate lth ‘ew aa ‘pn hate ‘Eira OH ee ey tem nner ‘naan aol jr cn te to 9c "ant coe ope ei me emma oa, Cote ples A Wierik prepyws w chromatografybbuloss ymit tk plo mig cla pm do predkosch weds ems Fava Zp #1 obese go mstepusacr % aie a iii tl da nego sida, system corpse ht (nays din poreceno io wich ceynit turks Ds alanis ayes Ho hom presse. 3m sa ink pin cay as Pewniirych wyrikiw dn anny wobec roiwors wrorcowey Bedtng) 1 warcowe) chromatognfovanch ne Ons samy are Oia pinay wynou wigs) ir 4082. Orme wpm Ry ne) sn i wymagn sor rownolegych chromogranin fepoveeeia edn] pik 0 rozravie rgd 2" oe agri bade) mi ma wr on pap ratogramis ) orhacenie soon bet Predaio na bbule oraz prose cog, nnn ns, ine a naa in tere mime a ae lana ey ober Ferra Loser st pce nomi ‘ts nh 9 sang oe os COnnaccenle akciowe pees ei polean ma wyoreie pam obriomanyc vedlug sabnrrigna Iso pocklniana » audios, potroeai ich me waste Fon eated ibay, lug nipeniedimirouoramisubxtanch nyarwions Erzbarwng) oraz na oenacoci siizinsbsanc cea Pomoe HOTS tare nelktatcrometrs, ecb jo west pam dane rei Rg, Far peek cnae {waorooweny © srosoket I STesat ae Aakowe) pocterachat, W najpomyiesjyeh marunkach wikia s 40 aed 42%ene do wpe) leet pow rou par BinacoOH. wn ‘ere nt ee aia aa oat egy ainoxwenon Ho Seo ee sprosskowanego bis: g ses anne Doki oftKG (2-6 Seer cams robin ino piven sa, zae 2 ol S51 Sr tr Hl mn aly pas nyo, Zatoions Wier dew cae w emp 1N6C pre 24 godt Do hiro yes i emer ih aay pune wc, Ary pryr7za nase Seren nl uoeg roworu HCL iia 2 420 ml wosy destlue ve Patek 10 ln, Pervee 60 ml miesaniny a ti soso non ty a a on face mr jor oct in ni a el. Aso fa ‘sig dokladnie} na dhursee) drodze ich wedriwki. ae NERY ako rent (ey er a -wome] chromatografii bibatowe) jednewymiarowe) = Wytemaie I epee eaten Nests Wt ae reneged ceo bes oe BS om mart ee oo ol wags mamas pt) mk Ne Ra neh 3 cn of eae 125 ot ST Seep amachonn octamer ontera enentrsie Os ‘ety Aur sre malo ror hone ‘ote rrp no Pare uaa) 3-4 ane pel ae ees ony patos cmos 1 a 1.0 seek oid 319 med any sate ps ok 18 snd SOHO 051510 iy mety rl ——a———————ce Pc yaaa Ey ae eerste roy on et ietememeemenmiteemi grrr: nc.” Unter eto sneeoenya shen pac (5 rortwr nn" feniaos Ne crmtoyran resto Algo od instar Cys 1° a Al PT MeV Phe, Lew le. (CRAAAE Helicon (Beat 69: Oznacranie wsutcongy anindvan mts diseroana (SEAR! CeomatngafaWlona jomynrons mbltyin RNA eH) Cremations ory na tench DNA 45. CHROMATOGRAFIA CIENKOWARSTWONA 4451, Podstay teoreyezne Se ee en ee mein ie skye rie weber Seen 4 bike wrench ees Creeoss ee oneFS eens an anise fy pena wn ta ss si aes SRA Siac psa wae nk Te deere. WC Be tie aioe ub acctonen) om gumowe 1 plat 1 mee poskiace en Com! Zavat ainn patton ria prystosowanego sro ‘fuser ds po pm do pk pra rates 300 0 of primi. Palla 6 Neh ow spel apart. 8 Aoinieie chromatogram, Ua rx \Wyotniecromatogamsjepodlumenin. slim poe ata ab ‘ose wajce ma chranstogramic arb Zeavaghan aye speyskanie pt substasjam tora zante plc Zs a Pod sortujepromienie UY, eo awicmaa se w psx ernych plum na wien le (Gal warsinasavicrata Jot suban ores. puoi Ce 1 Pochtnianc wystgpue opi Yo sprskanis pus wywsencem mp tor ‘Moray w metanok (etd sosowa ign season Peon Wywotywaceami mop tye wyike subane vane © cmap ule) do bare poszenyeh Bp win: Poa saa swat substance o bard slam dann one kas (80, HNO, mew Sechromowa, Do maj) umeanyh wots sn Ag an Duran whose onan smiion le tuna, ERO, w HaSO, ‘rar SbCIy 1 SDC, scrgnewshazne do w)kywans karen ‘etodo enol oraz mglowodorow «pea cypee waruy. Czasem po spy ete J ole ces jen. micszaniny Barwsikow yi alia ako sprawdzian pow dang) substan ot Bei amt sie sige of Mey aban wen KH exyncik, po nr Di dan so doiwie wsaza Male Fo te) sr ARS ae Wars nd Mein Govan rnc powina Dts sun ox ata Ay doko wily, ly rece ents pei pow pena Ry loupszcalnik do low cher fy tency Tez teh, KUGre mos poke ayptovat a ico. Naty zwracac uwoes natch avieajaeych alkohol i hw. Wats 904 spa sya komory params rope! aie pores rozpunzeainki ust Siena arash Rod tek psi oe bmg Ung aowania vkladuw roinych push an simomictne wysyeenie atmosfery komory prs ihc ‘nie ramaceajy sie jnakie doi” it erm ols Krist ree carts ms P wywoliin jdentyikaci badanych oie sig ego proce sfotogrlo¥ re sporzadzenie diaporsty¥i™ vo me. Ct ree ick = sy a inn yeh chromatogeamon. Ui”Je cecepencain geese unde rorPIalek eae re fee as Ute a ma seo om wu b= ew Ament ton h or {xo sionego pomiara substan stom, mn miarodajne niz w chroma 3-1 laniis masigajace meted so orbwarraie le gem Sons pun, prstnowggc oy onc tn rmee © pebee Oumecranie stgienin © chatach jest to minds en Hiegmeryens Unqehsotsies fein on fF ienlezych warukach. W meoneh piped ok Sanng {454, Chromatogrataclenkowarstwows prodstow hydro gluttiony oraz niekoryeh ecnockrow Clete 412. Lolomancglotatons »dridy harsh ore rn be inatogratcay jeg bydolzatakvasoveg> dn Mey oprah ein once a See ire aan eke oy pee erm Bag hay dol ae ae aa Selah tne Stsaneess na m aeons = paar aioe, le iSeances So Per one rortwors CCI,CG es fae es tO rortworu hac ess a im roaloiyé merkaptyd mictrion, Moreen HS te aparato Kipp i osc enkowarstwowe) MErkaptyd wy mete Meter para merkaptyda miediowee0 eh [pb | dodaé 0.3 ml 2M rociwou temp. 105°C proce 24 godz Fo Thydoizat pectic lsciowo do Pa unieis w eksyalooe Inydrlet x do uzyskania oY ob Ne doch a mastgpie dodat 05 mal H,0.Th Sesame ehromaogramy, Rownolesle ¥ Preparata waoecowego glutton! wykoaanie: [Prayeptoasiectromatogean Hiey(ok Sem tsi) a patna) pany wae ee AEE cece seen ore re ten ta ieee Co meme oii rk ate tet et ed uae nt [pare me) ego case zu. do wren 2 Nanosenie substan Lin srt vse brbtsego bok pith 2a pomocy sto sscuperoane wrdwnych odlegloiciach maniee po Sy tHntaminowego. 2 ponado hydrolets n Sat mevapiode mone dato Fy mans soy nag Stumieniem powicirra Nx pornym tra rn on bale ore plamy suncrami ub mapas costes Pe” ome SAH orp 3 bes 2 ey py, eat owceo pepe itn 1 des 4. 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Zosaly wyktye u ily Some, Pri, Ase re me Laem Nt Rule fa =" Min ae ho iowa) tubo ja pra rare ami avatoica lb alksbnten ac pm hn eg och ahh rac eee sa TERE eae aja opon em take ulus piscenionego. Wigkvnt sens nuataoe rumastea | ove w dodze mtabolzmo reps ‘te aly pcm py terpenidow. W przypudk (> ‘hat jo beng ph wks sak toroteyeznego. Thabo ‘ang a sim flog maa seo rasosowanie paki: oceans GREEAIRE WArwanc slain glitika (H Wagner, S. Bladt 1996 w 4) ae iy nes sp say: bey, cso ‘echey| meine Mae at EE petee, Peele i benlozocunalinaa css iy aa is ae enki cing and vhicwoki erases, lea Team 2 elma Naim fm 8 o (Geen NG) Ormacranie N-Lebconeg am orocs druwymarowechrmatopty enna > 4.6. FILTRACIA ZeLoWa Pramdsiny przlom w wchnce chromatogacn Porath Flin zastosowawseciowaoe poche desea ke arp ‘xatecock delstrany sala sg poste satoiegs npromacis to abomonon ‘Sephacryl wales ’ 464, Niektére sta molekulrne Saphader. 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Nak Acad. Sci. USA. 81 many. Uwaia sig, Ze w reabot akrouyeh agarozy tworn st apy tyne cr Supchmin we om ne 5 Rytoomony RNA, rnin DNA, ‘auminogen yer mins Faso ignore 4 nna wy, cea tpn, pt, Hen Spee 1 Wal A iae Gore oa eo Seam FF sen She a dese almost, Cm Seba 1 ase tao Staten ten sqnove inane Seoeat at a oa igand alk iat mes 80 | Tai aan NN pp i Sasa ope ais den ya aes ey gn a AE a eee Ph a ee tas ons sate ah efor 10 $e eta mo ln Sees ng) Rew eee al ene et peranenneas poars ar Se re 2 we ets eat ed cine in SSIES es ye ec Sect Dot SS ah Sa MS ors sas 2a prs) Helse 1M bulorem boranon) 3} rte 01 M bre scanowym airs | ase nae Maren branes 0 Sagat oda bakestacmes 2 ' i : Pinal ainlysh 1 a0 ST IB Ba. 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