Rencontres de lingnierie cologique en Bretagne

Ple Phytoremdiation
Titre Contact Socit / Organisme N0

TREATMENT OF WASTEWATER BY PHYTOREMEDIATION IN ARID AREA

Mohamed SAGGA Mounir

Laboratoire ECOBIO (CNRS, UR1, OSUR)

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PHYTOREMEDIATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS)

Anne-Sophie DUMAS

Laboratoire ECOBIO (CNRS, UR1, OSUR)

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STRESS RESPONSE TO POLYCYCLIC AROMATIC HYDROCARBON OF GLYCOPHYTES AND HALOPHYTES PLANT MODELS

MOEZ SHIRI

Laboratoire ECOBIO (CNRS, UR1, OSUR)

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23 janvier 2013 Carrefour des Gestions Locales de lEau

Treatment of wastewater by phytoremediation in arid area

Mohamed Mounir SAGGA1, Moez SHIRI2, Anne-Sophie DUMAS2, Ryad DJERIBI3 and Abdelhak
2 EL AMRANI

1 Ouargla University (Algeria) 2 Rennes1 University (France)/ ECOBIO Laboratory 3 Annaba University (Algeria)

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Arid climate

Macrophytes Phyto-purification

Domestic wastewater

Organic load
Pic.1

Research topic

Captions
Pic.1 Schematic design of the processing station
by macrophytes

- Ecotoxicology - Ecological restoration - Phytoremediation

Pic. 2

Input-output balance for BOD5 (Biological Demand of Oxygen ) balance for COD (Chemical Demand of Oxygen ) Variation of phenanthrene (enter, center and Exit)

Pic. 3 Input-output Pic. 4

Activities and Expertise

Design, construction and control of Wet Areas Wastewater Treatment,

Pic. 2

Pic. 3

Pic. 4

Image 3

Abstract
The aim of this study was the monitoring of the effectiveness of a system of sewage from domestic waste through the use of macrophytes in an arid climate. The experimental system was designed as fellow; the duration of treatment was 07 days (Pic.1). The reactor effluent through the basin by gravity with a flow of 4 L/min. The retention time in the decanter was 60 hours. Irrigation frequency is weekly. The parameters studied are: (TSS, BOD5, COD, EC, pH), nutrients (total nitrogen: total phosphorus and TKN: TP) and microbiological parameters. The results showed encouraging performance, decline in organic load and microbiologic load (TSS: 95.75%, BOD5: 91.15%, COD: 91.92%, PT: 24.90% pH 7.5).

Thematic topic
The arid climate and vegetation gives specificity to Saharan regions, which are characterized by a typical ecological fragility, this requires caution in approaches and choices to deal with environmental problems. phytoremediation approach may be used to clean up several sources of pollution, the challenge of this project was to exploit the flora of these regions and their potential to serve this choice (Pic.1).

Our Analyzes allowed us to determine the potential of WWG system. A new approach was developed with the laboratory of ECOBIO for the treatment of PAHs. The species studied showed high capability to reduce the concentration of PAHs (Pic. 4) These preliminary results open opportunities for broader uses of Saharan plants for phytoremediation.

Problematic

The project was led and determined to control the potential of a water treatment system by macrophytes (plants that grown in the study area), and study the behavior of Selected species without forgetting the quality of the water obtained after treatment (PIC. 2, 3 & 4) .

The project partners are:

- Ministry of Water Resources (Algeria) - National Sanitation Office (ONA) - Wastewater Garden (WWG). - APC Tmacine - University of Rennes 1

Subjects of study

Selected plant can reduce pollution levels to acceptable levels? The cleaning up capability is more or less stable throughout the year?

Coordonnes
CNRS UMR 6553 6553 Ecobio: Ecosystme,Biodiversit, Evolution Campus de Beaulieu, CS 742005 35042 Rennes Cedex, France Mohamed Mounir SAGGA/ Abdelhak El Amrani abdelhak.elamrani@univ-rennes1.fr mohamed-mounir.saggai@univ-rennes1.fr

Les Rencontres de lingnierie cologique en Bretagne, au 14e Carrefour des Gestions Locales de lEau, le 23 Janvier 2013 Rennes

Phytoremediation of polycyclic aromatic hydrocarbons (PAHs)

Anne-Sophie Dumas1, Richard Berthom2, Moez Shiri1, Mohamed Mounir Sagga1 and Abdelhak El Amrani1
1CNRS,

Universit de Rennes 1, UMR 6553, Ecosystmes-Biodiversit-Evolution, 35042 Rennes cedex, France, 2URGV, UMR INRA 1165 - CNRS 8114 - UEVE, 2, Rue Gaston Cremieux, CP5708, 91057 Evry cedex, France.

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Phytoremediation

PAH

Field trial

Molecular mechanisms

Plants

Green technology

Fields of Research
o o o

Activities
o o o

Decontamination of PAH-contaminated sites Green/ sustainable technologies Interface environmental stress-genome

Design and monitoring of PAH phytoremediation sites Molecular biology, in-vitro culture, GC-MS analysis Transcriptomics , metabolomics

Projects:

In-vitro analysis of PAHs effects on model plant, Arabidopsis thaliana, to understand how plants cope with PAHs stress at different scales (Fig 3.) :

Plant development in PAH contaminated media PAH effects on genome expression (1) and on metabolome at short-term (30 min to 24h) PAH quantification in plants (2) and localization of PAH in plants. Some additives, such as sucrose, enhance plant xenobiotics: is it appliable to PAHs? tolerance to some

Design and monitoring of a full-scale field trial of PAH phytoremediation: comparison between several plant species, impact of bringing a uncontaminated soil with its micro-organisms and worms on the rates of PAH degradation (3)

Issues
o o

How to optimize in-situ phytoremediation of PAH? What are the processes of detoxification of PAHs in plants?

Studies
o

Possible projects for the future

Testing other species and a mix of several
species (trees and contaminated soil grass) on a PAH

Create remediation systems combining plants, micro-organisms and agricultural practices (amendments, tillage) that are adapted to the site, the type of contaminant Identify and optimize the mechanisms of plants PAH metabolism Use our knowledge on detoxification systems in plants and microorganisms to enhance phytoremediation by creating or selecting plants species that are more tolerant and more efficient

o o

Create a guideline that will help in the set-up of a phytoremediation system Determination of the adapted species Establishing a timeline of treatment

Collaborations (1) URGV, INRA-CNRS, Evry, France (2) Gosciences, CNRS - University of Rennes 1, Rennes, France (3) Axson (formerly Bs Coatings), Aubevoye, France.

UMR 6553 Ecobio- Universit de Rennes 1- CNRS Campus de Beaulieu, Avenue du Gnral Leclerc 35042 RENNES Cedex France http://ecobio.univ-rennes1.fr/news.php

Contact Anne-Sophie Dumas (PhD student) - Abdelhak El Amrani (Supervisor) +33 (0)2 23 23 58 26 anne-sophie.dumas@univ-rennes1.fr Les Rencontres de lingnierie cologique en Bretagne 14e Carrefour des Gestions Locales de lEau, 23 Janvier 2013 - Rennes

Stress response to polycyclic aromatic hydrocarbon of glycophytes and Halophytes plant models (Arabidopsis thaliana, Thellungiella salsuginea)
Moez Shiri1, Med Mounir Sagga, Anne-Sophie Dumas, Chedly Abdelly1 and Abdelhak El Amrani1
1CNRS,

Universit de Rennes 1, UMR 6553, Ecosystmes-Biodiversit-Evolution, quipe Mcanismes lOrigine, LPE, laboratoire des plantes extrmophiles de la Biodiversit, 35042 Rennes cedex, France

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Polycyclic aromatic hydrocarbon

phytoremediation

Oxidative stress

Abiotic stress

Glycophyte

Halophyte

Knowledge - skills
studies of the mechanism of tolerance to abiotic stress.

Response of plants to PAH stress

Research project
o o o o o

hydrocarbon pollution functional genomics of phytoremediation Bioremediation and extremophile plants Activities and Expertise treatment of contaminated sites by organic pollution and heavy metals Application of biological processes
Image 4 Image 1

Project This thesis project is a collaboration between Rennes university and the Laboratory of Biotechnology and Extremophiles Plant of Borj Cedria center. It will study the physiological, biochemical and molecular responses of plants to abiotic stress. This study will focus on how plants may clean up contaminated environment.

Hypothesis
Extremophile plants have significant potential in terms of adaptation and tolerance. They have already proven their ability to grow in extreme conditions, and were used to desalinate and decontaminate soil polluted by heavy metal. These traits may help extremophile plants to clean up PAHcontaminated sites.

Issues
o

Identify the mechanisms of degradation and sequestration of PAHs in extremophile plants. Identify genes involved in stress tolerance towards PAHs Isolate the gene or genes responsible for PAHs degradation and overexpress them in plant in order to increase the remedial competitiveness

o o

UMR 6553 Ecobio Avenue du Gnral Leclerc Campus de Beaulieu 35042 RENNES Cedex - France 33.(0)2.23.23.58.26 http://ecobio.univ-rennes1.fr

Moez Shiri/ Abdelhak El Amrani Campus Beaulieu, bt 14a, p.122 CS 74205 - 35042 RENNES CEDEX 02.23.23.58.26 moez.shiri@univ-rennes1.f.fr abdelhak.elamrani@univ-rennes1.fr

Les Rencontres de lingnierie cologique en Bretagne, au 14e Carrefour des Gestions Locales de lEau, le 23 Janvier 2013 Rennes