ESTIMATION OF NICKEL WITH

DIMETHYLGLYOXIME(DMG)
Nickel (II) was estimated in sample by the reaction between nickel and
Dimethylglyoxime (DMG); forming the Ni-DMG pink color complex on the TLC foils. It
was done by taking spots of samples (2µL of each concentration) with help of
micropipette on the TLC foils impregnated with 1% DMG.Each spot was scanned using
“Flat-bed scanner”. Density of each concentration colored spot was taken by
“Densitometry” software programmed in PC.Ten spots were taken for each concentration
and reproducibility of each concentration was determined by plotting reproducibility
curve .RSD and 95% confidence interval (95%C.I ) of each concentration was noted. The
data obtained by each concentration is given below.
ESTIMATION OF NICKEL (II) WITH DIMETHYLGLYOXIME
For 100 ppm of Ni (II) Sample

Cons
Serial # (ppm) Optical density
1 100 109.8
2 100 109.9
3 100 109
4 100 109.2
5 100 108
6 100 111
7 100 109.8
8 100 109.09
9 100 112.09
10 100 112
 Mean =109.98
RSD = 1.209 %
95% C.I=109.98±0.6651

Reproducibility curve of 100ppm conc of Ni(II)

sample

114
112
optical density

110
108
106
104
1 2 3 4 5 6 7 8 9 10
no of obs

For 95 ppm of Ni (II) sample

Serial# conc. (ppm) optical density

1 95 105.8
2 95 106
3 95 105.9
4 95 104.9
5 95 104
6 95 105
7 95 105.09
8 95 104.98
9 95 103.998
10 95 105

Mean = 105.0668
RSD=0.668%
95% C.I=105.0668±0.3451
 Reproducibility curve of 95 ppm conc
of Ni(II)sample

108

optical density
106
104
102
1 2 3 4 5 6 7 8 9 10
no of obs

For 90ppm of Ni (II) sample

Serial# conc. ppm optical density
1 90 99.98
2 90 100
3 90 99.01
4 90 99.05
5 90 100
6 90 100.5
7 90 101
8 90 99
9 90 99.8
10 90 101
Mean=99.934
RSD=0.755%
95% C.I=99.934±0.3775

Reproducibility curve for 90 ppm conc of Ni(II)

sample

102
optical density

101
100
99
98
1 2 3 4 5 6 7 8 9 10
no of obs

For 85 ppm of Ni (II) sample

Serial # conc.(ppm) Optical density

1 85 94.01
 2 85 93.9
3 85 93.8
4 85 94.6
5 85 93.98
6 85 94
7 85 94.01
8 85 94.9
9 85 93.9
10 85 94.09
Mean=94.119
RSD=0.371%
95%C.I=94.119±0.1855

Reproducibility curve for 85ppm conc

for Ni(II) sample.

95
optical density

94.5

94

93.5

93
1 2 3 4 5 6 7 8 9 10
no of obs

For 80 ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 80 89
2 80 88.01
3 80 88.09
4 80 90.8
5 80 89.98
6 80 88.98
7 80 88.09
8 80 88.07
9 80 88.98
10 80 90
Mean=89
RSD= 1.104863%
95%C.I=89±0.492
 Reproducibility curve for 80ppm conc
of Ni(II) sample

92

optical density
90

88
86
1 2 3 4 5 6 7 8 9 10
no of obs

For 75 ppm 0f Ni (II) sample

Serial # conc.(ppm) optical density
1 75 83.1
2 75 84.01
3 75 82.5
4 75 83.6
5 75 81.9
6 75 82.8
7 75 82.09
8 75 81.99
9 75 84.01
10 75 84
Mean=83
RSD=1.044%
95%C.I=83±0.4333

Reproducibility curve for 75 ppm conc

of Ni(II) sample

85
optical density

84
83
82
81
80
1 2 3 4 5 6 7 8 9 10
no of obs
 For 70ppm of Ni (II) sample
Serial # Conc.(ppm) optical density
1 70 78.02
2 70 78.08
3 70 78.5
4 70 79.07
5 70 78
6 70 78.09
7 70 77.9
8 70 78.05
9 70 77
10 70 77.32
Mean=78.003
RSD=0.726%
95%C.I=78.003±0.2831

Reproducibility curve for 70 ppm conc

of Ni(II) sample

80
optical density

79
78
77
76
75
1 2 3 4 5 6 7 8 9 10
no of obs

For 65 ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 65 72.5
2 65 72.02
3 65 70.9
4 65 72.56
5 65 71.9
6 65 72.01
7 65 71.05
8 65 72.5
9 65 71.9
10 65 72.6
Mean=71.99
RSD=0.84%
95%C.I=71.99±0.3031
 Reproducibility curve for 65 ppm conc of
Ni(II) sample

73
72.5

optical density
72
71.5
71
70.5
70
1 2 3 4 5 6 7 8 9 10
no of obs

For 60ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 60 66.5
2 60 67
3 60 66.5
4 60 65.9
5 60 67.1
6 60 64.9
7 60 66.9
8 60 66.3
9 60 65.9
10 60 67
Mean=66.4
RSD=1.034%
95%C.I=66.4±0.3432

Reproducibility curve for 60 ppm conc

of Ni(II) sample

67.5
67
optical density

66.5
66
65.5
65
64.5
64
63.5
1 2 3 4 5 6 7 8 9 10
no of obs
 For 55ppm of Ni (II) sample
Serial # conc.(ppm) optical density
1 55 60
2 55 60.87
3 55 61.9
4 55 62
5 55 62
6 55 59
7 55 60.4
8 55 62.8
9 55 62
10 55 60.8
Mean=61.2
RSD=1.899%
95%C.I=61.2±0.581

Reproducibility curve for 55ppm conc of

Ni(II) sample

64
optical density

62
60
58
56
1 2 3 4 5 6 7 8 9 10
no of obs

For 50ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 50 54.6
2 50 53
3 50 55.6
4 50 55.12
5 50 54.6
6 50 55
7 50 54
8 50 55
9 50 58
10 50 55.4
Mean=55.032
RSD=2.334%
95%C.I=55.032±0.642
For 45 ppm of Ni (II) sample
 Serial # conc.(ppm) optical density
1 45 49
2 45 49.3
3 45 50
4 45 50.2
5 45 51
6 45 49.7
7 45 50.9
8 45 50.32
9 45 50.12
10 45 49.8
Mean=50.034
RSD=1.26%
95%C.I=50.034±0.31514

Reproducibility curve for 45 ppm conc of

Ni(II) sample

52
51
optical density

50
49
48
1 2 3 4 5 6 7 8 9 10
no of obs

For 40 ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 40 44.9
2 40 45
3 40 44.5
4 40 45.7
5 40 45.4
6 40 44.8
7 40 44
8 40 45.8
9 40 45
10 40 45.2
Mean=45.03
RSD=1.198%
95%C.I=45.03±0.27
 Reproducibility curve for 40 ppm conc
of Ni(II) sample

46

optical density
45

44

43
1 2 3 4 5 6 7 8 9 10
no of obs

For 35 ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 35 37
2 35 41.5
3 35 39.09
4 35 40
5 35 42.2
6 35 39
7 35 41.3
8 35 40.6
9 35 41.8
10 35 38
Mean=40.05
RSD=4.34%
95%C.I=40.05±0.87

Reproducibility curve for 35 ppm

conc of Ni(II) sample

45
optical density

40
35
30
1 2 3 4 5 6 7 8 9 10
no of obs
 For 30 ppm of Ni (II) sample

Serial # conc.(ppm) optical density

1 30 32.5
2 30 32
3 30 33.9
4 30 32
5 30 33
6 30 33.4
7 30 33.1
8 30 33.2
9 30 33.1
10 30 33.8
Mean=33
RSD=2%
95%C.I=33±0.33

Reproducibility curve for 30ppm

conc of Ni(II)sample

35
optical density

34
33
32
31
1 2 3 4 5 6 7 8 9 10
no of obs

For 25 ppm of Ni (II) sample.

Serial # conc.(ppm) optical density
1 25 28.1
2 25 27.5
3 25 27.9
4 25 28
5 25 29.1
6 25 30
7 25 28.5
8 25 27
9 25 26
10 25 28
Mean=28.01
RSD=3.89%
95%C.I=28.01±0.55
 Reproducibility curve for 25 ppm of
Ni(II) sample

32

optical density
30
28
26
24
1 2 3 4 5 6 7 8 9 10
no of obs

For 20 ppm of Ni (II) sample

Serial # conc.(ppm) optical density
1 20 22.4
2 20 21.5
3 20 21.6
4 20 22.5
5 20 22.9
6 20 21.5
7 20 21.8
8 20 21.6
9 20 22.8
10 20 21.4
Mean=22
RSD=2.65%
95%C.I=22±0.2925
 Reproducibility curve for 20 ppm
of Ni(II) sample

23.5
23

optical density
22.5
22
21.5
21
20.5
1 2 3 4 5 6 7 8 9 10
no of obs

For 15 ppm of Ni (II) sample.

Serial # conc.(ppm) optical density
1 15 16.8
2 15 17
3 15 16.5
4 15 16
5 15 17.5
6 15 16.8
7 15 16.8
8 15 17
9 15 17.1
10 15 17.5
Mean=16.9
RSD=2.63%
95%C.I=16.9±0.2224

Reproducibility curve for 15 ppm

of Ni(II) sample

18
Optical density

17.5
17
16.5
16
15.5
15
1 2 3 4 5 6 7 8 9 10
no of obs

For 10 ppm of Ni (II) sample.

Serial # conc.(ppm) optical density
 1 10 8
2 10 10.1
3 10 10.5
4 10 5
5 10 15
6 10 9.9
7 10 15
8 10 10.2
9 10 11
10 10 11.1
Mean=10.58
RSD=27.85%
95%C.I=10.58±1.5

Reproducibility curve for 10 ppm

of Ni(II) sample

20
optical density

15

10

0
1 2 3 4 5 6 7 8 9 10
no of obs

FINAL GRAPH BETWEEN CONCENTRATION AND OPTICAL

DENSITY
Serial # Conc.(ppm) Optical Density
1 10 10.58
2 15 16.9
3 20 22
4 25 28.01
5 30 33
6 35 40.049
7 40 45.03
8 45 50.034
9 50 55.032
10 55 61.177
11 60 66.4
12 65 71.99
13 70 78.003
14 75 83
15 80 89
16 85 94.119
17 90 99.934
18 95 105.0668
19 100 109.988
 Graph betw een concentration and optical
density

120

R2 = 0.9998
100

80

optical density
60

40

20

0
10
20

30
40
50

60
70
80

90

0
10
Concentration (ppm )