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Bio Section 02
Time (sec)
20
40
60
Toothpicks
Hydrolyzed
80
100
120
4. Graph the results plotting Product Formed (the total number of
toothpicks broken) vs. Time (20s, 40s, 60s, 80s...) on the grid below.
Now calculate the rate of enzyme action in toothpicks per second for each
60s interval for example, how many toothpicks were broken after 1
minute, 2 minutes, 3 minutes, etc.
Concentration of
Toothpicks
Time
(sec)
10
20
20
20
40
60
80
100
20
20
20
20
Toothpicks
Hydrolyzed
3. Remove the broken toothpicks and repeat with concentrations of 20, 30, 40, etc.
up to 100 toothpicks (i.e., generate 10 data points). Record your data in the table
above.
4. Graph the results by plotting Reaction Rate (toothpicks broken in 20
seconds) vs. Substrate Concentration (10, 20, 30, 40 ... 100). Dont forget to
title your graph and to label the axes. Each member of the group must do this.
Time (sec)
Temperature
10
Normal
10
Low
Toothpicks
Hydrolyzed
4. Calculate the rate of enzyme action in toothpicks per second for both
the low temperature and normal temperature conditions. Compare the two
rates which is higher?
What did you learn about enzymes and the way they act in Part A, about
the rate of product formation?
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What did you learn about enzymes and the way they act in Part B, about
the reaction rate and substrate concentration?
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What did you learn about enzymes and the way they act in Part C, about
the way the reaction rate is influence by temperature?
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The activity we just did used toothpicks as a model of enzyme activity.
Models arent perfect, though. What are the good parts and bad parts of
using this toothpickase model to learn about enzymes?
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6. Before handing in your lab, make sure that you have:
i) included a proper title for each graph, and labeled both the x and y axis
ii) answered the questions & completed the calculations
iii) put your name on the front page and make sure everything is stapled
together.