Model Test Paper 2013 B.Sc.-II Biotech.

Immunology
Question No. 1 is compulsory. Answer five questions in all, selecting one question from each pair. 1. Fill in the blanks:a) _____________ immunity is associated with specificity and memory. b) T helper cells express the membrane glycoprotein molecules ________ and are restricted to recognizing antigen bound to __________ on antigen presenting cell. c) The primary role of natural killer cells is the destruction of __________ and _________. d) _____________ and _____________ are the cells of the immune system that phagocytose and degrade antigens. e) __________ has a bilobed nucleus and granulated cytoplasm that stains with the __________. f) ___________ is the transplant of tissue from one person to another person, for example ____________. g) IgA is dimeric antibody and is primarily in secretions such as ________, ___________ and ____________. h) ___________ and __________ cells play an important role in the development of allergic reactions. i) The synthesis of T cells takes place in ____________ and maturation in ___________. j) Type I hypersensitivity can be exemplified by __________, _________ and ___________. 10X1 2. Define non-specific or innate immunity. Describe different types of barriers or lines of defences comprising innate immunity. Or Write short notes on any two: a) Natural Killer cells b) Lymph node c) Granulocytes 5+5 2+8

3. Discuss the distinguishing features of five different classes of immunoglobulins. 10

Or Describe briefly any two of the following:a) Radioimmunoassay b) Hybridoma technology c) Cytosolic pathway of antigen processing and presentation 5+5

4. What is complement system? Explain in detail the alternative pathway of complement system. Or Write short notes on any two:a) Type I hypersensitivity b) General properties of cytokines c) Antibody dependent cell mediated cytotoxicity 5. Describe the defense mechanism of host against bacterial infections. Or Write short notes on any two:a) Types of tumour antigens b) Autoimmune disorders c) Vaccines 5+5

2+8

10

Model Test Paper B.Sc. Biotech - I Microbiology

Time: 3 Hrs.

MM: 50

Question No. 1 is compulsory. Answer five questions in all, selecting one question from each pair. 1. Fill in the blanks:a) __________ are the micro-organisms which normally grow in hot acidic conditions. b) Monera kingdom includes all the ____________ organisms. c)Prokaryotes consist of ____________ type of ribosome. d) Viruses that infect bacterial cells are called ___________. e) Bacteria that can live in the presence of oxygen are called __________, and bacteria that can live in the absence of oxygen are called __________ f) According to the rules applied to the scientific naming of a biological organism, the ________________ is always capitalized. g) Our system of scientific nomenclature is called _____________ nomeclature. h) Bergey's Manual includes information on two domains _________ and

____________. i) Define Carboxysomes. j) Define species. 1 X 10=10

2. Describe in detail the points of difference between prokaryotic and eukaryotic cell with suitable diagrams. Or Write short notes on any two: a) Whittakers five kingdom classification b) Viruses c) Fungi 5+5 2+8

3. Describe in detail the ultra structure of bacterial cell. Or Describe briefly any two of the following:a) Cell wall of gram +ve and gram ve bacteria b) Structure and formation of endospores in prokaryotes c) Various types of Plasmids

10

5+5

4. Write short notes on:a) Conjugation b) Effect of pH and temperature on bacterial growth c) Continuous culture of microbes Or Write short notes on:a) Aerobic and anaerobic growth b) Generalized and specialized transduction c) Bacterial Growth curve 5. Describe the following: a) Kreb Cycle b) Secondary metabolites production by Microorganisms Or Write short notes on any two:a) Bioleaching b) Biogas c) Biopesticides 5+5

3+3+4

Model Test Paper B.Sc. Biotech - II Production of Recombinant Molecules Time: 3 Hrs. MM: 50 Attempt five questions in all, including Question No. 1 which is compulsory, selecting one question from each section. Q1. Answer the following:

a) __________ is an exonuclease that removes nucleotides from both strands of a double stranded molecule. b) Cosmid is hybrid between _________ and _________. c) AFLP stands for __________. d) BAC stands for __________. e) ___________ is the technique which is utilized for determination of nucleotides in a DNA molecule. f) The procedure by which billions of copies of a sequence of DNA can be made in a few hours is called the ___________ reaction. g) _____________ is a strong inhibitor to arrest the growth of cells at metaphase. h) _____________ enzyme helps in addition of phosphate group at 5 end of DNA molecule. i) In 1975, scientists convened in Asilomar, California and: i) Determined that restriction enzymes could cut DNA ii) Created the first transgenic animals iii) Reviewed the use of drugs produced by recombinant DNA technology iv) Drew up guidelines to regulate recombinant DNA technology j) The transgenic animals are used to produce drugs through their milk, blood or urine. This area is known as:i) Pharming ii) Hybridoma technology iii) Gene therapy

SECTION A Q2 Explain the following: a) Restriction endonuclease b) Reverse transcriptase c) Terminal deoxynucleotidyl transferase d) Polynucleotidyl transferase e) DNA ligase Q3 Describe in brief: a) Cosmid 5X2=10

b) YAC c) Transposons 3+3+4=10

SECTION B Q4 What is cDNA library? Explain in detail the construction of cDNA library. Q5 Explain the following: a) Ti plasmid b) Particle gun method c) Embryonic stem cell SECTION C Q6 How insulin and growth hormone can be synthesized with the help of recombinant DNA technology. Q7 Explain the following (any two): a) Promoters used in expression vector b) Fusion protein tags and purification tags SECTION D Q8 Explain in detail that how the purity and activity of over expressed protein can be determined. Q9 Explain the following any two: a) b) c) Baculovirus as an expression system Production of inclusion bodies and solublising insoluble proteins Yeast as experimental model system for over expression of proteins 5+5=10 5+5=10 10 3+3+4=10 10

Model Test Paper B.Sc. Biotech - III Plant Tissue Culture Time: 3 Hrs. MM: 50 Attempt five questions in all, including Question No. 1 which is compulsory, selecting one question from each section. Q1. Answer the following: 1. Plant tissue culture technique is a redefined method of ________ a) Hybridization

b) Vegetative Propagation c) Asexual Reproduction d) Selection 2. Polyethylene glycol is a) Fusogenic chemical b) Electrofusion stimulant c) Callus stimulant d) Differentiation stimulant 3. Somatic hybridization is achieved through a) Grafting b) Protoplast fusion c) Conjugation d) Recombinant DNA technology 4. The enzymes required to obtain wall-free / naked protoplasts are a) Cellulase and Proteinase b) Cellulase and Pectinase c) Cellulase and amylase d) Amylase and Pectinase 5. The first transgenic crop was a) Pea b) Tobacco c) Flax d) Cotton 6. A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.

a) microshoot b) medium c) explant d) scion 7. Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with a) cellulotyic enzymes b) pectolytic enzymes c) both cellulotyic and pectolytic enzymes d) proteolytic enzymes 8. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation? a) Multiplication of sexually derived sterile hybrids b) Less multiplication of disease free plants c) Storage and transportation of propagates d) Both (b) and (c) 9. What is meant by 'Organ culture' ?

a) Maintenance alive of a whole organ, after removal from the organism by partial

10.

a) b) c) d)

immersion in a nutrient fluid b) Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal c) Cultivation of organs in a laboratory through the synthesis of tissues d) The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body Which method of plant propagation involves the use of girdling? Grafting Cuttings Layering Micropropagation 10X1=10 10

Q2 Define the components and their composition of plant tissue culture media. Q3 Define the following: a) Surface sterilization technique b) Somatic embryogenesis Section - B Q4 Explain in detail the process of isolation and culturing techniques of single cell.

5+5

10

Q5 Describe in detail the process of isolation of somaclonal variants along with their application. 10

Section - C Q6 Describe the process micropropagation. Explain in detail along with its applications. 10 Q7 Explain in detail the steps involved in haploid production along with its major applications. 10 Section - D Q8 Explain the process of protoplast isolation, culture and its fusion for the formation of somatic hybrids. 10 Q9 How disease free plants can be produced with the help of tissue culture. 10

Model Test Paper M.Sc. (Previous) Biotechnology Virology and Immunology Time: 2 Hrs. MM: 50

Attempt five questions out of nine. Question No. 1 compulsory. All questions carry equal marks. Q1. Select the correct choice: 1. The mononuclear phagocyte system does not include: a) Monocytes b) Kupffer cells
c) Kidney mesangial cells

d)Endothelial cells

2. A polymorphonuclear neutrophil (PMN): a) Is a bone marrow stem cell c) Contains microbicidal cytoplasmic granules b) Is closely similar to a mast cell
d) Is not a professional phagocytic cell.

3. One principal function of the Class I and Class II MHC complex proteins is to a) transduce the signal to the T-cell interior following antigen binding b) mediate immunoglobulin class switching c) present antigen for recognition by the T-cell antigen receptor d) stimulate production of interleukins 4. All of the following are true about antibodies, EXCEPT which one? a) They fix complement c) They are glycoproteins b) They occur on the surface of B-lymphocyte d)They predominate the primary immune response to antigen

e) They are molecule with a single, defined amino acid sequence. 5. Antigen-antibody reactions can result in the following EXCEPT: a) Agglutination b) Complement fixation c) Virus neutralization

d) Allergic reactions e) Inflammatory reactions 6. Antibodies are distinct from antigens because: a) they can only be proteins c) they are not normally recognized as "foreign" b) they are only produced by B-cells d) they are only produced by T-cells

7. Bacteriophages that can enter into stable long-term relationships with their hosts are called: a) Lytic phages e) Temperate phage 8. a common polyhedral capsid shape of viruses is: a) cube b) icosahedrons c) pyramid d) sphere b) Defective phages c) Virulent phages d) Lazy phages

9. Which of the following viruses does not have an envelope? a) falvivirus b) Tobacco mosaic virus c) Corona virus d) Rubivirus

10. Viral replication within cell is inhibited by: a) IL-13 b) IL-7 c) IFNs d) IL-4 10

Q2. Describe the structural organisation of bacteriophages. Give brief details of X 174 and lambda bacteriophages. Q3. Explain the following any two: a) Physical and Chemical Method of viral assay b) Viral Vaccines 5+5

c) DNA viruses Q4. Explain the following any two: a) Maturation and activation of T lymphocytes b) Radioimmuoassay c) Hypersensitivity Q5. Write a brief account of any two: a) CTLs mediated cytotoxicity b) Hybridoma technology c) RNA viruses

5+5

5+5

5+5

Q6. Name and explain the different types of transplant? Also explain in detail the different stages of rejection of transplant. Q7. Write a brief account of: a) Autoimmune disorders b) Antigen processing and presentation Q8. Write a brief account of: a) Autoimmune disorders b) Antigen processing and presentation Q9. Write a brief account of: a) Immune response to bacterial infection b) AIDS 5+5

2+8

5+5

5+5