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Bou 'ill then: 1# enrich and isolate soil bacteria from sieved soil samples on special selection agar plates "C -rotocol A#, and in a parallel e1perimental set up !# observe bacterial cellulolytic activity in soil on cellulose filter discs plates "C -rotocol B# follo'ing the t'o protocols belo'.
7abl". Li t o! &no'n c"llulolytic oil bact"ria Bact"ria Na$" C*aract"ri tic >onsporulating, slender irregular rod shaped "coryneform# bacteria9 Dram )o iti#"9 A"robic or facultative anaerobic9 Eften motile9 ?onve1, yello' colonies
on peptone yeast agar
Ob "r#"d or I olat"d
"chec& mar&#
/emp range: !) %)o? >on photosynthetic short cyclindrical rods9 Dram n"gati#" A"robic or facultative anaerobic9 2otility "gliding# *avy edged, depressed "agar# colonies9 sho' spherical or oval
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9"r)"to i)*on
Clo tridiu$ )8 e.g. C. thermocellum "positive control bacterium in the 4io!5) lab# e.g. C. cellulolyticum e.g. C. cellobioparum
/emp. @ange: 15 L)o? Straight or curved rod shaped bacterium9 Dram n"gati#"9 A"robic "E! dependent#9 2otile9 ?atalase positive9 Produce fluorescent pigments "pyocyanins# ?ellulolytic9 /emp. @ange: %5o? ?ylindrical rod shaped bacteria9 Dram n"gati#"9 Strictly a"robic9 2otile "gliding#
My;obact"ria )8 e.g. Sorangium cellulosum e.g. Myxococcus xanthus e.g. M. sp. ALe.g. Stigmatella aurantiaca
3orms orange or bro'n colored my1ospores and fruiting bodies 'ith sporangioles9 "optical refraction#9 Decompose cellulose "e.g. filter paper#9 ?ellulolytic and chitinolytic activity
produce beta 1,( endoglucanase "?elA gene# 8
Acidot*"r$u c"llulolyticu
Butyro#ibrio )8
Str")to$yc"
)8
Sho' lichenoid leathery flat colonies and build hyphae as 'ell as air mycelia9 sporophores 'hich form N% spores9 3orm pigments "veget. cells O aerial mycelia#9 ?olonies 'ith typical soil smell9
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e.g. Microbispora bispora e.g. Streptomyces !SM"# halstedii e.g. $hermospora fusca
?ellulolytic9
produce beta 1,( endoglucanase "?asAA?elA1 gene#
?ellulolytic9
produces beta 1,( endoglucanase "E! gene#
0" ult 7abl". %n&no'n 2 ________________ Date Performed Test e%&% 'eth(l red _____________ Table # Result ne&at )e Comment a#ter 24 h ncubat "n n N* ____________________ Student Name Expected character !t c! "# dent # ed bacter um$
1+
$ acc"rd n& t" *er&e(,! 'anual "# Determ nat )e *acter "l"&( -9th Ed t "n.
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