act:
Gundruk is an important fermented
vegetable food in the local diet of the Sikkim
Himalayas which is prepared at individual household.
Present study improves the preservation and
nutritional value of a traditional fermented food –
Gundruk by various dehydration processes. The raw
materials used for the preparation of gundruk are rich
in carotenes and vitamin C. Carotene content per 100
gram of the cabbage is 120 microgram, radish leaves
is 13000 micro gram and mustard leaves is 2622
microgram. Vitamin C content of cabbage is 124 mg
and radish leaves is 106 mg. During the traditional
process of gundruk fermentation, there is 90% loss in
carotenes and most of the vitamin C is also lost. This
is mainly due to sun drying of the fermented leaves.
Improved methods of drying might reduce the
vitamin loss. The study concludes that introduction of
gundruk can be taken up as a new product
development which will not only confer nutritional
benefits to the people but also help in utilizing the
otherwise wasted vegetable leaves and can be
introduced globally.
Original Title
Process Improvement to Enhance the Nutritional Quality of Indian Fermented Food (Gundruk)
act:
Gundruk is an important fermented
vegetable food in the local diet of the Sikkim
Himalayas which is prepared at individual household.
Present study improves the preservation and
nutritional value of a traditional fermented food –
Gundruk by various dehydration processes. The raw
materials used for the preparation of gundruk are rich
in carotenes and vitamin C. Carotene content per 100
gram of the cabbage is 120 microgram, radish leaves
is 13000 micro gram and mustard leaves is 2622
microgram. Vitamin C content of cabbage is 124 mg
and radish leaves is 106 mg. During the traditional
process of gundruk fermentation, there is 90% loss in
carotenes and most of the vitamin C is also lost. This
is mainly due to sun drying of the fermented leaves.
Improved methods of drying might reduce the
vitamin loss. The study concludes that introduction of
gundruk can be taken up as a new product
development which will not only confer nutritional
benefits to the people but also help in utilizing the
otherwise wasted vegetable leaves and can be
introduced globally.
act:
Gundruk is an important fermented
vegetable food in the local diet of the Sikkim
Himalayas which is prepared at individual household.
Present study improves the preservation and
nutritional value of a traditional fermented food –
Gundruk by various dehydration processes. The raw
materials used for the preparation of gundruk are rich
in carotenes and vitamin C. Carotene content per 100
gram of the cabbage is 120 microgram, radish leaves
is 13000 micro gram and mustard leaves is 2622
microgram. Vitamin C content of cabbage is 124 mg
and radish leaves is 106 mg. During the traditional
process of gundruk fermentation, there is 90% loss in
carotenes and most of the vitamin C is also lost. This
is mainly due to sun drying of the fermented leaves.
Improved methods of drying might reduce the
vitamin loss. The study concludes that introduction of
gundruk can be taken up as a new product
development which will not only confer nutritional
benefits to the people but also help in utilizing the
otherwise wasted vegetable leaves and can be
introduced globally.
Process Improvement to Enhance the Nutritional Quality of Indian Fermented Food (Gundruk) Feby Lucose, K. Sundar and Prathapkumar Halady Shetty* Department of Food Science and Technology, Pondicherry University, India shetty.fst@pondiuni.edu.in Abstract: Gundruk is an important fermented vegetable food in the local diet of the Sikkim Himalayas which is prepared at individual household. Present study improves the preservation and nutritional value of a traditional fermented food Gundruk by various dehydration processes. The raw materials used for the preparation of gundruk are rich in carotenes and vitamin C. Carotene content per 100 gram of the cabbage is 120 microgram, radish leaves is 13000 micro gram and mustard leaves is 2622 microgram. Vitamin C content of cabbage is 124 mg and radish leaves is 106 mg. During the traditional process of gundruk fermentation, there is 90% loss in carotenes and most of the vitamin C is also lost. This is mainly due to sun drying of the fermented leaves. Improved methods of drying might reduce the vitamin loss. The study concludes that introduction of gundruk can be taken up as a new product development which will not only confer nutritional benefits to the people but also help in utilizing the otherwise wasted vegetable leaves and can be introduced globally.
1. INTRODUCTION Traditional foods form an integral part of cultural, religious as well as regional identity of a population and have been evolved as the result of tradition and empirical experiences of generations over a period. These foods play an important role in food and nutritional security of these populations, especially in the rural and semi urban areas. As a result of globalization and better socio economic status, consumers have varied lifestyle and consumption preferences. This has reduced the consumption of certain traditionally consumed products at the household level. However, many of these products have tremendous nutritional and health value. In this connection, value addition to these traditional products by making them widely acceptable and also to enhance their nutritive and health benefits through technological intervention is extremely important. Commercialisation of traditional foods not only has positive effects on rural economies, will also play a role in preventing the vanishing of these traditional foods. . According to UNESCO, traditional foods are slowly getting extinct and as many as are already extinct. Protection of traditional foods allows the protection of cultural heritage, consumers, and local producers. It allows job creation, and especially an increase of womens contribution to the economy. It guarantees production according to food safety regulations, and contributes to the promotion of a country [1]. Among traditional foods sold worldwide, Beaujolais Nouvea wine and Quiche Lorraine from France, Kimchi from Korea, Arepas from Venezuela, Feijoada from Brazil, Silig from Saudi Arabia sushi, natto from Japan, idli, dosa, dhokla of India, pizza, pasta, ricotta of Italy can be given as examples. Most of the traditional foods connected with each country are actually the fermented foods produced and consumed regionally in that country. Fermented foods appreciate worldwide popularity as attractive, wholesome and nutritive components of our diet. These foods produced on large scale employing International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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variety of ingredients and manufacturing practises [1]. Fermentation is also of economic importance in areas or for populations where preservation technologies such as cold storage and other techniques cannot be used for lack of resources or facilities. In such situations fermentation can be an affordable technology, which if applied correctly, results in the safe preservation of foods [2]. With technological intervention, it is possible to enhance the nutritional quality of traditional foods leading to reduction in the loss of valuable vitamins and other nutritionally important component. Sun drying is traditionally used as the major preservation method throughout the world. However, sun drying also results in the substantial loss of valuable nutritional components. There are many modern methods available for drying of foods like, freeze drying makes better preservation of nutrients of the food material. Gundruk is one of the widely consumed fermented foods in the North East parts of India and Nepal. Gundruk is nutritionally very rich and is also produced from the vegetable components normally discarded during cooking process. However, gundruk, like many other fermented foods, does not fid appealing to consumers who traditionally consume it due to its specific fermented flavour [3]. In the present study, various modern drying methods are compared with respect to retention of nutrients and also formulation of new recipes making the product widely acceptable. 2. METERIALS AND METHODS 2.1. Preparation of Gundruk Taking into consideration the availability of vegetable leaves in the local market, two leaves were selected for the present study to prepare Gundruk (outer leaves of cabbage and cauliflower). The leaves were first wilted in a well-ventilated room for one day and then washed in normal tap water thoroughly and then rinsed in lukewarm water (30-40 C). The leaves were then spread out to dry under shade. Care should be taken not to dry the leaves completely but the leaves should be slightly moist for crushing. Crushing was done using a mortar and pestle. The veins of the leaves should be well crushed. The crushed leaves were then packed tightly in polyethylene zip pouches and transferred to air tight container. The leaves were allowed to ferment at 25 C for 5-7 days. The leaves should retain some amount of moisture during fermentation, or else undesirable growth of yeasts and molds will take place. So to prevent this, the leaves should not be left to dry completely off moisture before crushing and also a temperature of 25 C should be maintained during the fermentation period. 2.2. Drying The fermented leaves were then subjected to drying by three methods sun drying, hot air drying and freeze drying. In hot air drying, the leaves were dried at 50 C for four hours. Freeze drying was done using VIS-TRIS Wizard Freeze drier. Drying kinetics was studied for hot air oven dried cauliflower and cabbage Gundruk. The samples were filled in petri dishes uniformly and the weight of the petri dishes noted. The petri dishes were kept in preheated hot air oven at 50 C. After 30 minutes interval one set of petri dish is taken out, cooled and weighed to a constant weight. In another petri plate, same amount of sample is taken and kept in a hot air oven at 120 C for 2 hours and then cooled in a desiccator and when constant weight is recorded, it gives the bone dry weight of the samples. Moisture content of the sample is expressed as moisture content on dry basis (MCDB = moisture in sample/amount of bone dry matter) and drying rate (= MCDB / drying time).MCDB can be plotted against time in minutes to give Drying Curve. Drying Rate Curve is obtained by plotting drying rate against time. 2.3. Chemical analysis 2.3.1. Determination of Vitamin-C 25g of sample was extracted with aliquots of 6% H 3 PO 3 , filtered into a 100ml volumetric flask by decantation re extracted 3 times with few ml each time of H 3 PO 3 . Filtered into the same flask and made up to volume to 100ml. To 5ml of Vitamin-C extract, suitably diluted, add 5ml of 3% H 3 PO 3 and titrated International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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against the standard dye solution. Vitamin-C (mg/100g) is calculated from the below formula: Vitamin-C (mg/100g) = Titre value x Dye factor x Volume made up x 100 / Volume taken for titration x Sample weight 2.3.3. Antioxidant potential (AOP) AOP of the sample can be measured using DPPH method, a simple method that has been used to determine the antioxidant potential of foods using stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. 100 grams of sample is extracted using methanol (about 100ml) using orbital shaker. The methanol extract is concentrated to 5- 10 ml using rotary evaporator. 250 micro litre of this extract is taken in 100ml volumetric flask and volume made up using methanol. 100 micro litres of extracts was taken and the volume made up to 100 millilitre using methanol. 3ml DPPH solution is taken in a test tube and incubated in the dark for 30 minutes. After which, absorbance was measured at 517 nm. Methanol is taken as blank and absorbance set to zero in the beginning. Vitamin C (0.1mg/ml) was used as standard. Total Antioxidant Activity is calculated using the formula; TAA (%) = (Absorbance of control absorbance of sample) x 100/ (Absorbance of control) 2.3.4. Estimation of total carotenoids 10g of sample was extracted with acetone in mortar and pestle, decanted the supernatant and repeated the step with acetone till the residue becomes colourless. The extract is filtered into 250 ml volumetric flask and volume made up with acetone. 20 ml of ether was taken in a separating funnel and 25 ml of acetone extract was added, water was added from the side until water layer is free of all fat soluble pigment. Drain of water layer, wash the ether layer 5-10 times with 10ml portion of distilled water until the ether layer is free of acetone. The ether extract is carefully separated and scanned using UV visible spectrophotometer (Shimadzu) to find out the absorption maxima. Total carotene content was calculated using the formula: Total carotene content = (3.857x max volume made up x100) / sample weight 2.3.5. Estimation of acidity The acidity is determined in triplicate by taking an aliquot of the sample (10 ml) with distilled water (40 ml) and titrated with 0.1N NaOH by using 3 drops of phenolphthalein solution as indicator. The titre value is noted and acidity is calculated by using the standard formula (Ranganna, 1999). The results were expressed as percentage anhydrous lactic acid. Acidity (% lactic acid) = (6.4 x Titre value x Normality of NaOH) / weight of the sample 2.4. Product development Following three different products were developed incorporating Gundruk (cabbage and cauliflower, 1:1): Gundruk - groundnut chutney mix Gundruk dehydrated squid mix Gundruk dry prawn mix Gundruk - groundnut chutney mix: For preparing Gundruk-groundnut chutney powder, 200g of groundnut was roasted and powdered in a grinder. 15g of dried red chillies, fried in oil and ground was added to the groundnut powder and mixed thoroughly. Salt was added for taste. Gundruk (ground to fine powder) was then added at 20, 30 and 40% to different batches. Gundruk dehydrated squid mix: Squid was dehydrated at 70 o C for 4 hours, powdered and spices (red chilli powder, cumin seeds, pepper), Bengal gram flour and salt were added. To this Gundruk was fortified at 20, 30 and 40%. Gundruk dry prawn mix: 200g dry prawn (small) was roasted to golden brown, cooled crushed 15g of dried red chillies, dry roasted and ground was added to the groundnut powder, prawn powder added to this and mixed thoroughly. Salt was added for taste. Gundruk was then added at 20, 30 and 40% to different batches. Sensory evaluation of Gundruk (dried by the three different methods) products was done by a panel of ten judges. The parameters considered were colour, taste, odour, texture, appearance and over all acceptability. The panellists used 9 point hedonic scale to rate the individual attributes numerically. International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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Scores were assigned from like extremely to dislike extremely. 3. RESULTS Moisture content on dry basis was calculated for the samples at an interval of 30 minutes. The table 1 shows that there is a steady decrease in moisture content during the first 60 minutes. The change in moisture content is then nearly constant for the next 30 minutes, after which the moisture content decreases gradually. The table 1 shows that drying rate is at its peak within first 30 minutes and decreases gradually thereafter. 3.1. Product Recovery The recovery of Gundruk dried by the three different methods was estimated. The results are tabulated in table 2. Recovery after drying was seen highest in hot air oven drying for both cabbage and cauliflower leaves. Sun drying gave the least amount of product. This may be due to greater removal of moisture in this method when compared to the other two methods. 3.2. Moisture estimation Moisture content of fresh leaves, fermented, sundried, hot air oven dried and freeze dried leaves were measured. The results are presented in table 3. Sundried Gundruk was found to contain the least moisture. But all the dried Gundruk samples were found to contain moisture level within acceptable limits. 3.3. pH Estimation The pH of fermented leaves, sundried, hot air oven dried and freeze dried samples were determined. pH of the samples increased slightly on drying this may be due to reduction of lactic acid content on drying. The pH of all the samples was in the range of 4.64 to 5.26 thus, the product falls in the category of low acid foods (Table 4). 3.4. Acidity Acidity of the samples was determined by titration method, in terms of lactic acid as it is the predominant acid produced by LAB. Fermented leaves were found to have the highest acid content followed by freeze dried and sundried Gundruk samples. Acidity of hot air oven dried Gundruk sample was found to be the lowest (Table 5). Table 1: Moisture content on dry weight basis (MCDB) and drying rate of cabbage and cauliflower Gundruk samples Time (min) MCDB (g) Drying rate (g/min) Cabbage Cauliflower Cabbage Cauliflower 0 4.479 5.173 - - 30 2.85 3.651 0.095 0.1217 60 1.79 2.759 0.029 0.046 90 1.515 2.65 0.017 0.029 120 0.705 1.926 0.006 0.016 150 0.418 1.409 0.003 0.009 180 0.216 0.917 0.001 0.005 210 0.056 0.811 0.0003 0.004 240 0.013 0.417 - -
Table 2: Recovery of Gundruk during various modes of drying Method of drying Cauliflower Cabbage Sun drying 52% 51.56% Hot air drying 57.30% 59.10% International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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Freeze drying 54.02% 57.28%
Table 3: Mean and standard deviation values of moisture content Sample type Cabbage MeanStd dev Cabbage MeanStd dev Fermented leaves 83.81.1 82.11.2 Sundried Gundruk 14.61.2 14.30.5 Hot air dried Gundruk 17.90.8 17.20.5 Freeze dried Gundruk 16.20.2 16.60.4
Table 4: Mean and standard deviation values of pH Sample type Cauliflower Cabbage Mean Std dev Mean Std dev Fermented leaves 4.730.02 4.640.03 Sundried Gundruk 4.920.02 4.970.02 Hot air dried Gundruk 5.260.04 5.140.02 Freeze dried Gundruk 4.90.02 5.030.15
Table 5: Mean and standard deviation values of acidity (in % lactic acid) Sample type Cauliflower Cabbage Mean Std dev Mean Std dev Fermented leaves 0.530.01 0.510.01 Sundried Gundruk 0.460.02 0.470.13 Hot air dried Gundruk 0.430.12 0.410.02 Freeze dried Gundruk 0.460.03 0.430.01
3.5. Vitamin C analysis Vitamin C content was analysed for the samples to study which drying treatment helped in preserving the maximum amount of vitamin C leaves (Table 6). Vitamin being heat labile, is easily degraded when exposed to heat and oxygen. It also destroyed on crushing and cutting of leaves. Hence, some loss was seen in the vitamin C content when compared to that of raw fresh. Vitamin C loss was the highest in sun drying was 85.2% and 87.2% in cabbage and cauliflower leaves respectively (Table 7), which is expected as it is an uncontrolled method of drying. Freeze drying was found to be the most effective method of drying in terms of vitamin C loss, which was the least at 15.5% and 17% for cabbage and cauliflower leaves respectively. 3.6. Carotene content The carotene content of raw leaves, fermented leaves, sundried, hot air oven dried and freeze dried leaves were estimated and the values were analysed to determine the loss of carotene content of leaves during the various levels of processing. It was found that freeze drying preserved carotenes to the maximum. Fermentation resulted in some International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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loss of carotenes at 8.78% and 6.20% for cabbage and cauliflower leaves respectively. Sundried caused great loss in carotene content at 79.43% and 80.01% (Table 8 and 9). 3.7. Antioxidant Potential The antioxidant potential of Gundruk prepared by the three methods of drying were determined by DPPH method and expressed in terms of Total Antioxidant Activity (TAA) % (Table 10). 3.8. Product Development Three different products were developed incorporating Gundruk (cabbage and cauliflower, 1:1). They were: Gundruk - groundnut chutney mix Gundruk dehydrated squid mix Gundruk dry prawn mix Product 1: Gundruk groundnut chutney mix Three variations of the product was prepared and presented to a panel of ten judges. The three variants were: GG1 20% Gundruk + 80% groundnut mix GG2 30% Gundruk + 70% groundnut mix GG3 40% Gundruk + 60% groundnut mix GG4 Groundnut mix (Control) The results of the sensory evaluation, using nine point hedonic scale rating, are tabulated below: According to the results of the sensory evaluation, Gundruk groundnut mix at 20% Gundruk level was the accepted variant. GG3 with 40% Gundruk was found to have a slight bitter after taste as per the comments given by the panellists. Product 2: Groundnut dehydrated squid mix The three variants prepared were: GS1- 20% Gundruk + 80% dehydrated squid mix GS2 30% Gundruk + 70% dehydrated squid mix GS3 40% Gundruk + 60% dehydrated squid mix GS4 Dehydrated squid mix (Control) GGS1 with 20% Gundruk was well accepted by the panellists. GGS3 with 40% Gundruk scored least. Product 3: Gundruk dry prawn mix Three variations of the product were as follows: GP1- 20% Gundruk + 80% dry prawn mix GP2 30% Gundruk + 70% dry prawn mix GP3 40% Gundruk + 60% dry prawn mix GP4 dry prawn mix (Control) GP1 with 20% dry prawn scored the highest among the variants and was given a high overall acceptability score of 8. GP3 scored the least among the variants, but managed to score an overall acceptability of 7 points. 3.9. Sensory Evaluation of Gundruk samples Gundruk prepared by the three drying methods namely sun drying, hot air drying and freeze drying were evaluated for their sensory properties, in order to determine if there were any adverse effect on the sensory attributes of the mechanically dried Gundruk over the conventionally sun dried Gundruk. These samples were also compared with Gundruk prepared and bought from Nepal. The samples used were: SG sundried Gundruk HG hot air oven dried Gundruk FG freeze dried Gundruk NG Gundruk sample from Nepal (Control) In all the above samples cauliflower and cabbage Gundruk were mixed in 1:1 ratio. From the data shown above, it is evident that freeze dried Gundruk is more acceptable when compared to other samples. Gundruk when incorporated into other products was found to be more acceptable by the panellists when compared to 100% Gundruk (Table 11, 12, 13 and 14) and (Figure 1, 2, 3 and 4).
Table 6: Mean and standard deviation values for vitamin C content (mg/100g) Sample type Cabbage Cauliflower International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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Mean Std dev Mean Std dev Fresh leaves 101.871.01 48.270.65 Fermented leaves 94.10.65 43.60.7 Sundried Gundruk 15.180.3 6.10.1 Hot air dried Gundruk 57.140.15 25.410.4 Freeze dried Gundruk 86.41.87 39.770.3
Table 7: Vitamin C loss on processing (in %) Sample Cabbage Cauliflower Fermented leaves 8.43 9.7 Sundried Gundruk 85.2 87.2 Hot air oven dried Gundruk 44 47.3 Freeze dried Gundruk 15.5 17
Table 8: Total carotene content of the samples (g /100g) Sample Cabbage Cauliflower Raw leaves 4774.96 6232.44 Fermented leaves 4355.18 5840.3 Sundried Gundruk 982 1246.73 Hot air oven dried Gundruk 3409.41 4139.29 Freeze dried Gundruk 3994.62 5331.47
Table 9: Carotene loss on processing Sample Cabbage Cauliflower Fermented leaves 8.78 6.2 Sundried Gundruk 79.43 80.01 Hot air oven dried Gundruk 28.6 33.5 Freeze dried Gundruk 16.34 14.5
Table 10: Antioxidant Potential of the Gundruk samples Sample TAA (%) TAA (mean std dev) Standard antioxidant (Vitamin C) 94.8 93.99 0.88 Cauliflower, Sundried 13.66 13.30 0.34 Cauliflower, Hot air dried 15.8 15.52 0.25 Cauliflower, Freeze dried 24.63 25.53 1.11 Cabbage, Sundried 21.47 21.14 0.28 Cabbage, Hot air dried 31.22 30.88 0.31 International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
4. DISCUSSIONS Gundruk was prepared by fermentation of outer cabbage leaves and cauliflower leaves at 25o C for 5-7 days and then dried employing three different methods like sun drying, hot air oven drying and freeze drying, to study the effect of mechanical drying on the nutritional as well as sensory characteristics of Gundruk. Effect of the different methods of drying and also of fermentation on the vitamin C and carotene content of Gundruk was studied. Anti- Oxidant Potentials (AOP) of Gundruk prepared by the three drying methods were compared to identify the drying method that retained the highest AOP level. Vitamin C content of fresh cabbage outer leaves was found to be higher than that reported by previous studies, 32 mg/100 g and 27.32 mg/100 g in white cabbage. This difference may be because in the present outer leaves of green cabbage were used. Vitamin C loss was seen highest in sun drying (85.2% and 87.2% in cabbage and cauliflower Gundruk respectively), which is expected as it is an uncontrolled method of drying. Freeze drying was found to be the most effective method of drying in terms of vitamin C loss, which was the least at 15.5% and 17% for cabbage and International Journal of Human Genetics Medical Biotechnology and Microbiological Studies ISSN (Online) 2319-1732 : Volume 1 , Issue 2 , October 2012-10-12
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cauliflower leaves respectively. Similar results were observed by Ndawul et al. [4] in cow pea leaves, wherein open sun drying method caused the greatest vitamin C loss at 84%. In another study, Sun-dried leaves had the highest loss of vitamin C (90%), while Freeze-dried leaves, on the other hand, had the lowest vitamin C loss of 19% on jute leaves. Post fermented leaves suffered vitamin C loss at around 8% and 9% in cabbage and cauliflower leaves. In a study by Raci [5] on tarhana, a Turkish fermented cereal food, fermentation at 30 0C for 4 days was found to increase vitamin C content. Vitamin C loss in fermented cabbage and cauliflower leaves can be due to crushing step involved prior to in Gundruk preparation. Crushing and cutting of leaves lead to oxidative degradation of vitamin C. Freeze dried Gundruk was found to retain highest amount of carotenoids. Sun drying resulted in high loss of carotenes. A studied on the effect of hot air oven drying on the beta nutrient contents of the outer leaves of cabbage showed similar result at 50% retention in the beta carotene level and less than 40% retention in vitamin C [6]. The observations are also similar to that noted in cow pea leaves drying where open sun drying method caused the greatest beta carotene loss of 58% [4] and amaranthus leaves in which open sun drying caused 86.5% loss in beta- carotenes [7]. In another study, loss of beta carotene from green leafy vegetables such as mint, curry, gogu and amaranth, after drying was found to range from 24 to 40% in sun dried leaves and 6 to 25% in oven dried [8]. The Total Antioxidant Activities of sundried, hot air oven dried and freeze dried Gundruk samples were evaluated. In the case of cabbage and cauliflower Gundruk, freeze dried samples were found to show highest TAA. But the TAA of hot air dried Gundruk prepared from outer cabbage leaves were found to show lower TAA values than the TAA (94.05%) of the outer cabbage leaves dried at 70 C, studied by Nilnakara et al. [9]. However, the freeze dried cabbage Gundruk showed higher TAA value than the TAA (53.4 0.6%) of outer cabbage leaves measured by Ismail et al. [10] by using the ethanol extraction method. The pH, acidity and moisture content observed in the Gundruk samples were similar to the values observed by Tamang [3]. Evaluation of Gundruk conducted showed that Gundruk as such, to be eaten raw, was not very well accepted, because of the strong sour and bitter taste which was perceived to be unpleasant by the panel members. Gundruk was preferred to be combined with other ingredients that can mask the strong taste of Gundruk to develop products that will be acceptable but the local people. Taking this into consideration, three products incorporating Gundruk were prepared - Gundruk - groundnut chutney mix, gundruk dehydrated squid mix and Gundruk dry prawn mix. Among the three products developed, Gundruk- dry prawn mix scored the highest in the nine point hedonic scale rating with an overall acceptability score of 8 0.65. 5. CONCLUSION It can be concluded that the experiments carried out in this study showed that freeze drying and hot air oven drying help in better retention of nutrients like vitamin C, carotenes and also showed higher total antioxidant activities for the Gundruk samples when compared to the conventional sun drying that causes high loss in the nutrients. The study also showed that introduction of Gundruk in this part of India can be taken up as a new product development which will not only confer nutritional benefits to the people but also help in utilizing the otherwise wasted vegetable leaves. References 1. A.R. Sepaskhah, and M.M. Ghasemi, Every-other- furrow irrigation with different irrigation intervals for grain sorghum. Pak. J. Biol. Sci. 2008: vol. 11, pp. 1234-1239. 2. M. Albayrak, and E. Gunes, Traditional foods: Interaction between local and global foods in Turkey. African Journal of Business Management. 2010: vol. 4, pp. 555-561. 3. J.P. Tamang, Role of microorganisms in traditional fermented foods. Indian Food Industry. 1998: vol. 17, pp. 162-167.
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4. J. Ndawula, and J.D. Kabasa, Y.B. Byaruhanga, Alterations in fruit and vegetable beta-carotene and vitamin C content caused by open-sun drying, visqueen-covered and polyethylene-covered solar- dryers. African Health Sciences. 2004: vol. 4, pp. 125-30. 5. E. Raci, The effect of fermentation and drying on the water-soluble vitamin content of tarhana, a traditional Turkish cereal food. Food Chemistry. 2005: vol. 90, pp. 127-132. 6. Y. Tanongkankit, N. Chiewchan, and S. Devahastin, Effect of processing on antioxidants and their activity in dietary fiber powder from cabbage outer leaves. Drying Technology. 2010: vol. 28, pp. 1063-1071. 7. B.T. Kiremire, E. Musinguzi, J.F. Kikafunda, and F.B. Lukwago, Effects of vegetable drying techniques on nutrient content: A case study of south- western Uganda. African Journal of Food Agriculture Nutrition and Development. 2010: vol. 10, pp. 2587-2600. 8. V.A. Aletor, O.A. Adeogun, Nutrient and antinutrient components of some tropical leafy vegetables. Food Chemistry. 1995: vol. 53, pp. 375-379. 9. S. Nilnakara, N. Chiewchan, S. Devahastin, Production of antioxidant dietary fibre powder from cabbage outer leaves. Food and Bioproducts Processing. 2007: vol. 87, pp. 301-307. 10. A. Ismail, C. Sook Fun, Determination of vitamin C, -carotene and riboflavin contents in five green vegetables organically and conventionally grown. Malaysian Journal of Nutrition. 2003: vol. 9, pp. 31-39.
Effect of 1-Methylcyclopropene Concentration, Storage Temperature and Packaging On The Postharvest Quality of Mango (Mangifera Indica L.) Fruit Cv. Broken and Dausha
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