The comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in Owerri was carried out. The mean total aerobic plate count for the borehole water ranged from 2.48 ± 0.02 Log10cfu/mL to 2.58 ± 0.05 Log10cfu/mL while the tap water ranged from 2.00 ± 0.01 Log10cfu/mL to 2.42 ± 0.05 Log10cfu/mL. The mean coliform count for borehole and tap water ranged from 2.04 ± 0.02 Log10cfu/mL to 2.38 ± 0.10 Log10cfu/mL and 1.20 ± 0.03 Log10cfu/mL to 1.46 ± 0.30 Log10cfu/mL respectively. The mean fungal counts for borehole and tap water ranged from 1.95 ± 0.06 Log10cfu/mL to 2.20 ± 0.04 Log10cfu/mL and 1.65 ± 0.05 Log10cfu/mL to 2.24 ± 0.08 Log10cfu/mL respectively. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts for both the borehole and tap water samples were 0 ± 0.00 Log10cfu/mL respectively. The microorganisms isolated were Proteus sps, Staphylococcus aureus and Klebsiella sps Geotrichum sps, Aspergillus sps and Fusarium sps. The mean values for arsenic, barium, cadmium, mercury and nickel in both the borehole and tap water were <0.001 ± 0.00mg/mL. The mean values for the other metals in borehole and tap water were Chromium, 0.008 ± 0.002mg/L and <0.001 ± 0.00mg/L; Copper, 0.230 ± 0.019mg/L and 0.194 ± 0.012mg/L; iron, 0.915 ± 0.010mg/L and 0.542 ± 0.090mg/L; lead, 0.004 ± 0.002mg/L and <0.001 ± 0.00mg/L; manganese, 0.111 ± 0.009mg/L and 0.092 ± 0.010mg/L and zinc, 0.420 ± 0.030mg/L and 0.272 ± 0.020mg/L respectively. The result showed that the water samples were contaminated and should be treated before consumption.
Article Citation:
Eze VC and Okeke CO.
Comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in owerri, Imo State, Nigeria.
Journal of Research in Public Health (2012) 1(2): 047-055.
Full Text: http://jhealth.info/documents/PH0010.pdf
Original Title
Comparative Analysis of Microbiological and Heavy Metal characteristics of tap and borehole water in owerri, Imo State, Nigeria
The comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in Owerri was carried out. The mean total aerobic plate count for the borehole water ranged from 2.48 ± 0.02 Log10cfu/mL to 2.58 ± 0.05 Log10cfu/mL while the tap water ranged from 2.00 ± 0.01 Log10cfu/mL to 2.42 ± 0.05 Log10cfu/mL. The mean coliform count for borehole and tap water ranged from 2.04 ± 0.02 Log10cfu/mL to 2.38 ± 0.10 Log10cfu/mL and 1.20 ± 0.03 Log10cfu/mL to 1.46 ± 0.30 Log10cfu/mL respectively. The mean fungal counts for borehole and tap water ranged from 1.95 ± 0.06 Log10cfu/mL to 2.20 ± 0.04 Log10cfu/mL and 1.65 ± 0.05 Log10cfu/mL to 2.24 ± 0.08 Log10cfu/mL respectively. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts for both the borehole and tap water samples were 0 ± 0.00 Log10cfu/mL respectively. The microorganisms isolated were Proteus sps, Staphylococcus aureus and Klebsiella sps Geotrichum sps, Aspergillus sps and Fusarium sps. The mean values for arsenic, barium, cadmium, mercury and nickel in both the borehole and tap water were <0.001 ± 0.00mg/mL. The mean values for the other metals in borehole and tap water were Chromium, 0.008 ± 0.002mg/L and <0.001 ± 0.00mg/L; Copper, 0.230 ± 0.019mg/L and 0.194 ± 0.012mg/L; iron, 0.915 ± 0.010mg/L and 0.542 ± 0.090mg/L; lead, 0.004 ± 0.002mg/L and <0.001 ± 0.00mg/L; manganese, 0.111 ± 0.009mg/L and 0.092 ± 0.010mg/L and zinc, 0.420 ± 0.030mg/L and 0.272 ± 0.020mg/L respectively. The result showed that the water samples were contaminated and should be treated before consumption.
Article Citation:
Eze VC and Okeke CO.
Comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in owerri, Imo State, Nigeria.
Journal of Research in Public Health (2012) 1(2): 047-055.
Full Text: http://jhealth.info/documents/PH0010.pdf
The comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in Owerri was carried out. The mean total aerobic plate count for the borehole water ranged from 2.48 ± 0.02 Log10cfu/mL to 2.58 ± 0.05 Log10cfu/mL while the tap water ranged from 2.00 ± 0.01 Log10cfu/mL to 2.42 ± 0.05 Log10cfu/mL. The mean coliform count for borehole and tap water ranged from 2.04 ± 0.02 Log10cfu/mL to 2.38 ± 0.10 Log10cfu/mL and 1.20 ± 0.03 Log10cfu/mL to 1.46 ± 0.30 Log10cfu/mL respectively. The mean fungal counts for borehole and tap water ranged from 1.95 ± 0.06 Log10cfu/mL to 2.20 ± 0.04 Log10cfu/mL and 1.65 ± 0.05 Log10cfu/mL to 2.24 ± 0.08 Log10cfu/mL respectively. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts for both the borehole and tap water samples were 0 ± 0.00 Log10cfu/mL respectively. The microorganisms isolated were Proteus sps, Staphylococcus aureus and Klebsiella sps Geotrichum sps, Aspergillus sps and Fusarium sps. The mean values for arsenic, barium, cadmium, mercury and nickel in both the borehole and tap water were <0.001 ± 0.00mg/mL. The mean values for the other metals in borehole and tap water were Chromium, 0.008 ± 0.002mg/L and <0.001 ± 0.00mg/L; Copper, 0.230 ± 0.019mg/L and 0.194 ± 0.012mg/L; iron, 0.915 ± 0.010mg/L and 0.542 ± 0.090mg/L; lead, 0.004 ± 0.002mg/L and <0.001 ± 0.00mg/L; manganese, 0.111 ± 0.009mg/L and 0.092 ± 0.010mg/L and zinc, 0.420 ± 0.030mg/L and 0.272 ± 0.020mg/L respectively. The result showed that the water samples were contaminated and should be treated before consumption.
Article Citation:
Eze VC and Okeke CO.
Comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in owerri, Imo State, Nigeria.
Journal of Research in Public Health (2012) 1(2): 047-055.
Full Text: http://jhealth.info/documents/PH0010.pdf
Comparative analysis of microbiological and heavy metal
characteristics of tap and borehole water in owerri, Imo State, Nigeria
Keywords: Comparative, analysis, microbiological, heavy metal, tap, borehole, water. ABSTRACT:
The comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in Owerri was carried out. The mean total aerobic plate count for the borehole water ranged from 2.48 0.02 Log 10 cfu/mL to 2.58 0.05 Log 10 cfu/mL while the tap water ranged from 2.00 0.01 Log 10 cfu/mL to 2.42 0.05 Log 10 cfu/mL. The mean coliform count for borehole and tap water ranged from 2.04 0.02 Log 10 cfu/mL to 2.38 0.10 Log 10 cfu/mL and 1.20 0.03 Log 10 cfu/mL to 1.46 0.30 Log 10 cfu/mL respectively. The mean fungal counts for borehole and tap water ranged from 1.95 0.06 Log 10 cfu/mL to 2.20 0.04 Log 10 cfu/mL and 1.65 0.05 Log 10 cfu/mL to 2.24 0.08 Log 10 cfu/mL respectively. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts for both the borehole and tap water samples were 0 0.00 Log 10 cfu/mL respectively. The microorganisms isolated were Proteus sps, Staphylococcus aureus and Klebsiella sps Geotrichum sps, Aspergillus sps and Fusarium sps. The mean values for arsenic, barium, cadmium, mercury and nickel in both the borehole and tap water were <0.001 0.00mg/mL. The mean values for the other metals in borehole and tap water were Chromium, 0.008 0.002mg/L and <0.001 0.00mg/L; Copper, 0.230 0.019mg/L and 0.194 0.012mg/L; iron, 0.915 0.010mg/L and 0.542 0.090mg/L; lead, 0.004 0.002mg/L and <0.001 0.00mg/L; manganese, 0.111 0.009mg/L and 0.092 0.010mg/L and zinc, 0.420 0.030mg/L and 0.272 0.020mg/L respectively. The result showed that the water samples were contaminated and should be treated before consumption. 047-055 | JRPH | 2012 | Vol 1 | No 2 This article is governed by the Creative Commons Attribution License (http://creativecommons.org/ licenses/by/2.0), which gives permission for unrestricted use, non-commercial, distribution and reproduction in all medium, provided the original work is properly cited. www.jhealth.info Journal of Research in Public Health An International Scientific Research Journal Authors: Eze VC 1 and Okeke CO 2 .
Institution: 1. Department of Microbiology, Michael Okpara University of Agriculture, Umudike, PM.B.7267, Umuahia, Abia State, Nigeria.
2. Department of Microbiology, Madonna University, Elele Campus, Rivers State, Nigeria.
Corresponding author: Eze VC.
Email: mekus2020@gmail.com
Web Address: http://www.jhealth.info/ documents/PH0010.pdf.
Dates: Received: 15 Aug 2012 Accepted: 22 Sep 2012 Published: 30 Oct 2012 Article Citation: Eze VC and Okeke CO. Comparative analysis of microbiological and heavy metal characteristics of tap and borehole water in owerri, Imo State, Nigeria. Journal of Research in Public Health (2012) 1(2): 047-055 Original Research Journal of Research in Public Health J o u r n a l
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R e s e a r c h
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P u b l i c
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An International Scientific Research Journal
INTRODUCTION Drinking water or potable water is the water pure enough to be consumed or used with low risk of immediate or long term harm. The water supplied to households, commerce and industry in most developed countries is all of drinking water standard, although only a very small proportion is actually consumed or used in food preparation. Water is essential for the survival of all the organisms and has always been an important and life-sustaining drink to humans. Water composes approximately 70% of the human body by mass excluding fat. It is a crucial component of metabolic processes and serves as a solvent for many bodily solutes. Drinking water with a variety of qualities is bottled. Bottled water is sold for public consumption throughout the world. Humans in most parts of the world have inadequate access to potable water and use sources contaminated with disease vectors, pathogens or unacceptable levels of toxins or suspended solids. Drinking or using such water in food preparation leads to widespread acute and chronic illnesses and is a major cause of death and misery in many countries. Reduction of waterborne diseases is a major public health goal in developing countries (Greenhalgh, 2001; USEPA, 2005). The availability of water has become a critical and urgent problem in many developing countries This is a matter of great concern to families and communities depending on non-public water supply system (Okonko et al ., 2008). Increase in human population has exerted an enormous pressure on the provision of safe drinking water especially in the areas of the developing countries (Umeh et al., 2005). There is a great variation in the quality of water that comes out of our taps. Depending on the country and the region, water comes from different, sometimes mixed, sources - surface water, underground sources or even desalinated seawater, as is the case in the Middle East. It undergoes disinfection and chemical treatments, such as chlorine addition that affect the original taste of the water. The processes and level of monitoring vary greatly throughout the world. This water then flows through pipes made of different materials that date from different times and are hermetically sealed to varying degrees, all of which greatly influence the quality of the water that arrives in a home (Nestle Waters, 2009). Unsafe water is a global public health threat, placing persons at risk for a host of diarrheal and other diseases as well as chemical intoxication (Hughes and Koplan, 2005; Agbabiaka and Sule, 2010). Unsanitary water particularly has a devastating effect on young children in the developing world. Nearly 90% of diarrhoeal related deaths have been attributed to unsafe or inadequate water supplies and sanitation conditions affecting a large part of the worlds population. The most common contamination of raw water sources in most parts of the world is from human sewage and particularly human faecal pathogens and parasites. It was estimated in 2006, that waterborne diseases cause 1.8 million deaths each year and about 1.1 billion people lacked proper drinking water. It is then obvious that people in the developing world need to have access to good quality water in sufficient quantity, water purification technology and availability and distribution systems for water. The only source of water in many parts of the world are from small streams, which are often contaminated directly by sewage (WHO, 2005; Hughes and Koplan, 2005; CDC, 2006, Ihejirika, 2011). The principal objectives of municipal water are the production and distribution of safe water that is fit for human consumption (Lamikanra, 1999; Okonko et al., 2008). Recently in Nigeria drinking water is commercially available in easy-to-open 50-60mL polythene sacks Known as sachet/pure water Eze and Okeke,2012 048 Journal of Research in Public Health (2012) 1(2): 047-055 (Umeh et al., 2005). Confirmation with microbiological standard is of special interest because of the capacity of water to spread diseases within a large population. Although the standard varies from place to place, the objective is to reduce the possibility of spreading water borne disease in addition to being pleasant to drink, which implies that it must be wholesome and palatable in all respects (Edema et al., 2001; Okonko et al., 2008, Ihejirika et al.,2011). A collaborative interdisciplinary effort to ensure global access to safe water, basic sanitation and improved hygiene is the foundation for ending cycle of poverty and diseases (Hughes and Koplan, 2005). The aim of this study is therefore to analyze the microbiological and physicochemical characteristics of borehole and tap water sources in Owerri municipal, Imo State, Nigeria.
MATERIALS AND METHODS Collection of Samples The water samples were collected aseptically from 10 different boreholes and taps within the Owerri municipality using sterile containers. They were transported to the laboratory in an ice packed cooler and immediately analyzed on reaching the laboratory.
Chemical Reagents The chemical reagents employed in the study were of analytical grade and were products of BDH Chemicals, Pooles England and Sigma Chemical Company St. Louis Missouri, USA. The microbiological media used were products of Oxoid and DIFCO Laboratories, England. They included nutrient agar used for the estimation of total heterotrophic aerobic bacteria, purification of isolates and for stock culture; Sabouraud dextrose agar used for the isolation of fungi and MacConkey agar for the isolation of coliforms, eosin methylene blue agar for Escherichia coli, Salmonella-Shigella agar for Salmonella-Shigella count, thiosulphate citrate bile sucrose agar for Vibrio cholerae count and Sabouraud dextrose agar for fungal count. Enumeration of Total Heterotrophic Bacteria and Fungi The borehole and tap water samples were serially diluted in ten folds. Total viable heterotrophic aerobic counts were determined using pour plate technique. Then the molten nutrient agar, Sabouraud dextrose agar, MacConkey agar, eosin methylene blue agar, Salmonella-Shigella agar and thiosulphate citrate bile sucrose agar at 45C were poured into the Petri dishes containing 1 mL of the appropriate dilution for the isolation of the total heterotrophic bacteria and fungi, Eze and Okeke, 2012 Journal of Research in Public Health (2012) 1(2): 047-055 049
Log 10 cfu/mL Sample TAPC CC EC SSC VC FC A 2.49 0.03 2.04 0.02 0 0.00 0 0.00 0 0.00 2.08 0.27 B 2.58 0.05 2.38 0.10 0 0.00 0 0.00 0 0.00 2.20 0.20 C 2.48 0.02 2.32 0.06 0 0.00 0 0.00 0 0.00 2.08 0.22 D 2.52 0.32 2.34 0.20 0 0.00 0 0.00 0 0.00 1.95 0.23 E 2.53 0.04 2.26 0.05 0 0.00 0 0.00 0 0.00 2.11 0.07 F 2.45 0.02 2.20 0.05 0 0.00 0 0.00 0 0.00 1.95 0.06 G 2.57 0.08 2.34 0.20 0 0.00 0 0.00 0 0.00 2.20 0.40 H 2.51 0.06 2.36 0.02 0 0.00 0 0.00 0 0.00 2.04 0.02 I 2.54 0.20 2.28 0.02 0 0.00 0 0.00 0 0.00 2.08 0.09 J 2.56 0.07 2.24 0.02 0 0.00 0 0.00 0 0.00 2.18 0.10 Legend: TAPC = total aerobic plate count; CC = Coliform count; EC = Escherichia coli count; SSC = Salmonella-Shigella count; VC = Vibrio cholerae count; FC = Fungal count Table 1 Mean Counts of Microorganisms isolated from Borehole Water Samples
coliform, Escherichia coli, Salmonella-Shigella and Vibrio cholerae respectively. They were swirled to mix and colony counts were taken after incubating the plates at 30C for 48 h and preserved by sub culturing the bacterial isolates into nutrient agar slants which were used for biochemical tests. Characterization and Identification of Isolates Bacteria isolates were characterized and identified after studying the Gram reaction as well as cell micro morphology. Other tests performed were spore formation, motility, oxidase and catalase production; citrate utilization, oxidative/fermentation (O/F) utilization of glucose; indole and coagulase production, starch hydrolysis, sugar fermentation, methyl red-Voges Proskauer reaction and urease production. The tests were performed according to the methods of (Cheesbrough, 2005; Adeoye, 2007; Agwung-Fobellah and Kemajou, 2007; Ochei and Kolhatkar, 2007). Microbial identification was performed using the keys provided in the Bergeys Manual of Determinative Bacteriology (1994). Determination of Heavy Metals The heavy metals of the borehole and tap water samples were determined using Unicam atomic absorption spectrophotometer (Model 969, Unicam).
RESULTS The results of the microbiological and heavy metal characteristics of the borehole and tap water samples are shown in Tables 1 - 5. The mean counts of the microorganisms isolated from the borehole water samples are shown in Table-1. The total aerobic plate count ranged from 2.48 0.02 Log 10 cfu/mL to 2.58 0.05 Log 10 cfu/mL while the coliform count ranged from 2.04 0.02 Log 10 cfu/mL to 2.38 0.10 Log 10 cfu/mL. The fungal count ranged from 1.95 0.06 Log 10 cfu/mL to 2.20 0.04 Log 10 cfu/mL. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts were 0 0.00 Log 10 cfu/mL respectively. Table-2 shows the mean counts of the microorganisms isolated from the tap water samples. The total aerobic plate count ranged from 2.00 0.01 Log 10 cfu/mL to 2.42 0.05 Log 10 cfu/mL while the Coliform count ranged from 1.200.03 Log 10 cfu/mL to 1.46 0.30 Log 10 cfu/mL. The fungal count ranged from 1.65 0.05 Log 10 cfu/mL to 2.24 0.08 Log 10 cfu/mL. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts were 0 0.00 Log 10 cfu/mL and has no count on it. Eze and Okeke,2012 050 Journal of Research in Public Health (2012) 1(2): 047-055 Log 10 cfu/mL
Sample
TAPC
CC
EC
SSC
VC
FC K 2.28 0.03 1.30 0.03 0 0.00 0 0.00 0 0.00 1.85 0.06 L 2.35 0.30 1.34 0.05 0 0.00 0 0.00 0 0.00 1.95 0.20 M 2.25 0.04 1.28 0.20 0 0.00 0 0.00 0 0.00 2.04 0.30 N 2.00 0.01 1.24 0.04 0 0.00 0 0.00 0 0.00 1.90 0.06 O 2.10 0.02 1.46 0.30 0 0.00 0 0.00 0 0.00 1.75 0.10 P 2.22 0.20 1.30 0.08 0 0.00 0 0.00 0 0.00 1.65 0.05 Q 2.30 0.10 1.26 0.10 0 0.00 0 0.00 0 0.00 2.00 0.20 R 2.42 0.05 1.22 0.05 0 0.00 0 0.00 0 0.00 1.85 0.40 S 2.40 0.06 1.32 0.22 0 0.00 0 0.00 0 0.00 2.24 0.08 T 2.38 0.20 1.20 0.03 0 0.00 0 0.00 0 0.00 1.98 0.06 Legend: TAPC = total aerobic plate count; CC = Coliform count; EC = Escherichia coli count; SSC = Salmonella-Shigella count; VC = Vibrio cholerae count; FC = Fungal count Table 2 Mean Counts of Microorganisms isolated from Tap Water Samples The comparative mean counts of the microorganisms isolated from the borehole and tap water samples are shown in Table-3. The mean total aerobic plate counts for the borehole and tap water samples were 2.52 0.09 Log 10 cfu/mL and 2.27 0.21 Log 10 cfu/mL respectively. The coliform count for the borehole water sample was 2.28 0.07 Log 10 cfu/mL while the count for the tap water sample was 1.29 0.11Log 10 cfu/mL. The Escherichia coli, Salmonella-Shigella and Vibrio cholerae mean counts for both the borehole and tap water samples were 0 0.00cfu/mL and has no count in it . The ANOVA, P < 0.05 showed that there is significant difference in the total aerobic plate and coliform mean counts for both the borehole and tap water samples. The fungal counts for the borehole and tap water samples were 2.09 0.17 Lg 10 cfu/mL and 1.92 0.15 Lg 10 cfu/mL respectively. The ANOVA, P < 0.05 showed that there was no significant difference in the mean fungal count. Table-4 shows the microorganisms isolated and their percentage of occurrence. The microorganisms isolated and their percentage of occurrence in both the borehole and tap water were Proteus sps, 46.7% and 42.1%; Staphylococcus aureus, 16.7% and 26.3%; Klebsiella sps, 36.7% and 31.6%; Geotrichum sps, 31% and 36%; Aspergillus sps, 43% and 44%; Fusarium sps, 24% and 20% respectively. The mean values of the heavy metals in the borehole and tap water are shown in Table-5. The mean values for arsenic, barium, cadmium, mercury and nickel in both the borehole and tap water were <0.001 0.00mg/mL respectively. The mean values for the other metals in borehole and tap water were Chromium, 0.008 0.002mg/L and <0.001 0.00mg/L; copper, 0.230 0.019mg/L and 0.194 0.012mg/L; iron, 0.915 0.010mg/L and 0.542 0.090 mg/L; lead, 0.004 0.002mg/L and <0.001 0.00mg/L; manganese, 0.111 0.009mg/L and 0.092 0.010mg/L and zinc, 0.420 0.030mg/L and 0.272 0.020mg/L respectively.
DISCUSSION The water samples were analyzed for the presence of pathogenic and non pathogenic microorganisms and heavy metals, which was used to determine the level of contamination and the safety and Eze and Okeke, 2012 Journal of Research in Public Health (2012) 1(2): 047-055 051 Log 10 cfu/L Sample TAPC CC EC SSC VC FC Borehole 2.52 0.09 2.28 0.07 0 0.00 0 0.00 0 0.00 2.09 0.17 Tap 2.27 0.21 1.29 0.11 0 0.00 0 0.00 0 0.00 1.92 0.15 Table 3 The Comparative Mean Counts of Microorganisms isolated from the Borehole and Tap Water Samples Legend: TAPC = total aerobic plate count; CC = Coliform count; EC = Escherichia coli count; SSC = Salmonella-Shigella count; VC = Vibrio cholerae count; FC = Fungal count Microorganism No. of isolates borehole water No. of isolates tap water % Occurrence borehole water % Occurrence tap water Bacteria Proteus sps 14 16 46.7 42.1 Staphylococcus aureus 5 10 16.7 26.3 Klebsiella sps 11 12 36.7 31.6 Fungi Geotrichum sps 9 9 31 36 Aspergillus sps 13 11 43 44 Fusarium sps 7 5 24 20 Table 4 Microorganisms isolated from the Borehole and Tap water and their percentage occurrence
suitability of the water for human consumption (Geldreich, 2000; Agbabiaka and Sule, 2010). The high microbial counts showed that the borehole and tap water were polluted. The coliform values were very high and therefore were above the WHO limit for drinking water of 0MPN/100mL. The presence of coliform is suggestive of human faecal contamination which may be as a result of seepage into the broken water pipes and the boreholes. Coliforms are known to be the most widely used indicator organisms to determine the level of faecal pollution of the water system. It is also an indication of inadequate treatment or post-chlorination system (Okafor, 1999; Agbabiaka and Sule, 2010). The few microbial species isolated from the water samples may reflect depth and types of materials on the passage routes, the dissolved salt and general human activities especially those bordering on waste disposal system (Yates, 1985; Agwung et al., 2006). The consistent high level of total coliform and other microbial counts is an indication of poor microbiological quality of the water samples. It has been observed that sterile water devoid of microorganisms rarely exists except in the laboratory (Ogbulie and Akujiobi, 2006). The isolation of these bacteria in the water samples is an indicative of feaces or related pollution that may result from poor sanitary conditions especially during harvesting packaging storage and transport of the product. Staphylococcus aureus is a normal flora of the body and mucous membrane and a common aetiological agent of septic arthritis (Ellen and Sydney, 1990; Eze et al., 2008). The consumer is at risk of acquiring food borne diseases. Staphylococcus aureus is the major cause of staphylococcal food poisoning. The poisoning is characterized by diarrhea and vomiting (Singleton, 1995; Frazier and Westhoff, 2004; Eze et aI., 2008). The higher load of this organism in tap water than in borehole water samples may be as a result of handling of the taps and washing of hands or other parts of the body on the taps by individuals who carry it on their bodies as normal flora thereby leaving the organism on the surfaces of the taps. The fungi isolated showed that fungi could survive in water, which agrees with the finding that almost all classes of fungi have representatives in water (Okafor, 1999; Agwung et al., 2006). The heavy metal contents of both the borehole and tap water with exception of iron were within the WHO limit of potable water (WHO, 2006). The accumulation of this metal in the water should be minimized to avoid the toxic effect on human and other animal species. It was observed that the value of the iron was higher in tap water samples than in borehole water samples. This may be attributed to the corrosion of iron or steel pipes or other components of the plumbing system where the acidity of the water measured as pH is below 6.5. The reddish brown Eze and Okeke, 2012 052 Journal of Research in Public Health (2012) 1(2): 047-055 Parameter Borehole water Tap water WHO Standard Arsenic (mg/L) <0.001 0.00 <0.001 0.00 0.01 Barium (mg/L) <0.001 0.00 <0.001 0.00 0.7 Cadmium (mg/L) <0.001 0.00 <0.001 0.00 0.003 Chromium (mg/L) 0.008 0.002 <0.001 0.00 0.05 Copper (mg/L) 0.230 0.019 0.194 0.012 1-2 Iron (mg/L) 0.915 0.010 0.542 0.090 0.3 Lead (mg/L) 0.004 0.002 <0.001 0.00 0.01 Manganese (mg/L) 0.111 0.009 0.092 0.010 0.1-0.5 Mercury (mg/L) <0.001 0.00 <0.001 0.00 0.001 Nickel (mg/L) <0.001 0.00 <0.001 0.00 0.02 Zinc (mg/L) 0.420 0.030 0.272 0.020 3.0 Table 5 Mean values of the Heavy Metal Characteristics of the Borehole and Tap Water Samples precipitate or particles that appear and settle to the bottom of the tap water is as a result of the presence of dissolved ferrous iron. It also gives water a disagreeable metallic taste. The use of more resistant polyvinylchloride pipes to avert the problem is recommended (Eze and Okpokwasili, 2008; Kiely, 1998; Quinn, 2010). All the sampled water was contaminated with microorganisms and the levels of contamination were above the maximum permissible limits of World Health Organization (WHO, 2004). It then follows that these waters, not potable as water intended for human consumption should be free from colour, taste, hardness and microbial, chemical and other physical contaminants (Nwaugo et al., 2006; Okoli et al., 2005). Tap water should be regulated by law and treated to guarantee that it meets certain health criteria and should not be considered always suitable for drinking as the case in many developing countries. Several types of domestic filters are sold to that improve the characteristics of tap water. Depending on the type of filter, the protection it provides represents a more or less reliable barrier against chemical, mineral or bacteriological elements. Regular maintenance of this sort of equipment is crucial to prevent the growth of bacteria (Nestle Waters, 2009). It is therefore the responsibility of the government and the governed to see to the provision of safe, clear and potable water. The essence is to improve the public health standard of the people, which will increase productivity, efficiency and improved social status, but will result in low medical cost, reduced mortality rate and economic loss.
CONCLUSION The increase in the rate of indiscriminate dumping of refuge, sewage disposal and other human activities has lead to the contamination of our drinking water systems. These activities are of public health importance because of the diseases associated with such dumping. It is therefore necessary that all these activities should be controlled so that our drinking water is always be potable.
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