Herbal purple sweet potato (PSP) wine was prepared from purple-fleshed sweet potato (Ipomoea batatas L.) and 18 medicinal plant parts. Starch present in PSP was enzymatically saccharified to fermentable sugars. Medicinal plant parts were mixed to it at desirable quantities before subjected to fermentation.
Herbal purple sweet potato (PSP) wine was prepared from purple-fleshed sweet potato (Ipomoea batatas L.) and 18 medicinal plant parts. Starch present in PSP was enzymatically saccharified to fermentable sugars. Medicinal plant parts were mixed to it at desirable quantities before subjected to fermentation.
Herbal purple sweet potato (PSP) wine was prepared from purple-fleshed sweet potato (Ipomoea batatas L.) and 18 medicinal plant parts. Starch present in PSP was enzymatically saccharified to fermentable sugars. Medicinal plant parts were mixed to it at desirable quantities before subjected to fermentation.
SANDEEP K. PANDA 1 , MANAS R. SWAIN 2 , SRADHANJALI SINGH 3 and RAMESH C. RAY 1,4 1 Regional Centre of Central Tuber Crop Research Institute, Bhubaneswar, Orissa 751013, India 2 Department of Biotechnology, College of Engineering and Technology, Bhubaneswar, Orissa, India 3 Department of Bioresources Engineering, Institute of Minerals and Materials Technology, Bhubaneswar, Orissa, India 4 Corresponding author. TEL: +91-674-2470528; FAX: +91-674-2470528; EMAIL: rc_rayctcri@rediffmail.com Accepted for Publication January 19, 2012 doi:10.1111/j.1745-4549.2012.00681.x ABSTRACT A herbal purple sweet potato (PSP) wine was prepared from purple-eshed sweet potato (Ipomoea batatas L.) and 18 medicinal plant parts (fruits of ink nut, Indian gooseberry, garlic cinnamon, leaves of holy basil, night jasmine, Malabar nut, roots of belladonna, asparagus, rhizome of ginger, etc.) by fermenting with wine yeast, Saccharomyces cerevisiae. The starch present in PSP was enzymatically saccharied (using commercial thermostable enzymes Termamyl [0.2%] and Dextrozyme GA [1%]) to fermentable sugars, and the homogenized medicinal plant parts were mixed to it at desirable quantities before subjected to fermentation. The herbal wine had the following compositions: total soluble sugar (TSS), 4.0 Brix; starch, 0.24 g/ 100 mL; total sugar (TS), 0.95 g/100 mL; reducing sugar, 0.38 g/100 mL; titratable acidity (TA), 1.25 g tartaric acid/100 mL; phenol, 0.19 g (caffeic acid equivalent)/ 100 mL; anthocyanin, 59.90 mg/100 mL; lactic acid (LA), 1.92 mg/100 mL; ethanol, 8.61% v/v; and pH, 3.34. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of the wine was 51.35% at a dose of 250 mg/mL. The herbal wine thus pre- pared was presumed to contain the therapeutic and antioxidant properties of PSP as well as those of medicinal plant parts added as adjuncts. Principal component analy- sis reduced the 11 original analytical and proximate variables (TSS, reducing sugars, starch, TS, TA, pH, phenol, DPPH, LA, ethanol, anthocyanin) to four independent components, which accounted for 74.53% variations (PC1, 24.18%; PC2, 20.18%; PC3, 15.41%; PC4, 14.76%). PRACTICAL APPLICATIONS Herbal purple sweet potato (PSP) wine prepared fromanthocyanin-rich PSP and 18 different types of medicinal plant parts is a novel and unique product with ethanol content of 8.61% v/v. The wine is rich in antioxidants such as anthocyanin and phenols and presumably possesses biological active ingredients as remedies for common ailments like cold, cough, skin diseases and dysentery. INTRODUCTION Medicinal herbs and spices have been added to foods since ancient times, not only as avoring agents but also as folk medicine andfoodpreservatives (Beuchat 1994; Cutler 1995). These herbs prolong the storage life of foods and beverages by preventing spoilage through their antioxidant and bacterio- static or bactericidal activity (Beuchat and Golden 1989) and alsoenrichthe foods withphytonutrients, vitamins andessen- tial minerals. A number of studies have been reported on medicinal herbs used for production of wine either as sub- strate (rawmaterial) or as adjuncts. Soni et al. (2009) reported fermentation of Indian gooseberry (Emblica ofcinalis) fruits (having medicinal properties like diuretic, laxative, carmina- tive, stomachic, astringent, antidiarrheal, antihemorrhagic and antianemic) to wine. Aloe vera known to be a panacea has beenfermentedtowine (Pongparnchedtha andSuwanvisolkij 2011 http://www.aseanfood.info/scripts/count_article.asp). Journal of Food Processing and Preservation ISSN 1745-4549 1 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. Tea (Cammellia sinensis) leaves have been fermented to wine, having very good acceptance both on sensory and health attributes (Aroyeun et al. 2005). Herbal tea has been prepared by blending common South African medicinal herbs those are known for combating arthritis, constipation, hangover, cardiac andother diseases withtea andmarketedthe enriched tea (Bhat andMoskovitz 2009). Purple sweet potato (PSP) (Ipomoea batatas L.) is a special type of sweet potato, and there is high content of anthocyanin pigments in the roots of some sweet potato cultivars (Ray and Tomlins 2010). The anthocyanins from PSP are more stable than those fromstrawberry, red cabbage and other plants (Lu et al. 2010). Many researchers reported PSP anthocyanins could scavenge free radicals (Saigusa et al. 2005), attenuate liver dysfunction (Han et al. 2007), enhance memory func- tion (Wu et al. 2008), decrease blood sugar (Ray and Tomlins 2010) and lower insulin resistance (Kusano and Abe 2000) and inhibit cancer cell growth (Wang et al. 2006). In our pre- vious study, we have developed a red wine by fermenting the starchy PSP roots with Saccharomyces cerevisiae, and the wine has identical characteristics as that of commercial red (grape) wine (Ray et al. 2011). Infrared spectroscopy is an interesting tool for the analysis of beverages and foods to determine the functional groups (Lamet al. 2005). The analyses of food and beverages through Fourier transform infrared (FT-IR) have several advantages as it is rapid, nondestructive and reproducible. Principal component analysis (PCA) is a method used advantageously for the evaluation in the food product analysis (Arvanitoyan- nis and Tzouros 2005). PCAis used for the reduction of infor- mation on large number variables into a small set, while losing only a small amount of information (Hair et al. 1998). The major feature of this method is a reduction of the dimen- sionality in a set of variables by constructing an uncorrelated linear combination of them. The combinations are computed in such a way that the rst component accounts for the major part of variance, that is the major axis of the points in the P-dimensional space (Tzouros and Arvanitoyannis 2001). In this study, an attempt has been made to prepare an herbal wine using 18 different types of medicinal herbs and plant parts (Table 1) and PSP. Evaluation of the wine charac- teristics was carried out with reference to nutrients and proxi- mate composition. The results of the proximate composition were analyzedby multivariate statistical methods (correlation analysis and PCA). MATERIALS AND METHODS Medicinal Herbs and Plant Parts Healthy medicinal herbs and plant parts (Table 1) were selected from the local horticultural garden at Bhubaneswar, India, during the month of November 2009 (day tempera- ture, 28 2C and night temperature, 20 2C). Specic parts of the herbs (Table 1) to be used in wine making were thoroughly washed, sun-dried and crushed to get them in powder forms. PSP Roots Freshly harvested and unbruised PSP roots (var. ST-13) (starch, 178 g/kg; total sugar [TS], 25 g/kg; and fresh roots) were collected from the experimental farm of Regional Centre of Central Tuber Crops Research Institute (CTCRI), Bhubaneswar, during the month of November 2009 and immediately brought to the Microbiological Laboratory of the Regional Centre of CTCRI. The roots were used within 24 h after harvest. Wine Yeast The wine yeast, S. cerevisiae was maintained on potato dex- trose agar (PDA) slants at 4C in a refrigerator. This culture is regularly used in wine making in our laboratory (Choudhury and Ray 2007; Kumar et al. 2008). The yeast strain was pro- vided by Dr. E. Suresh, principal scientist (Microbiology), Division of Postharvest Technology, Indian Institute of Hor- ticultural Research, Bangalore, India. Preparation of Starter Culture One hundred grams of good quality sweet grapes (var. Banga- lore blue) was selected and washed under running tap water. The grapes were mashed in a mixer-cum-grinder (TTK Pres- tige Limited, Bangalore, India), and the juice was extracted using a juice squeezer. The volume of the juice (ltered through cotton cheese cloth) was measured, and an equal volume of water was added. The mixture was boiled for 1015 min on a hot plate and was cooled at room tempera- ture. After cooling, the grape juice was inoculated with S. cer- evisiae culture from the stock culture under laminar air ow (Klenzoides, Mumbai, India) and was incubated at 30C for 24 h to prepare the starter culture. Enzymes for Saccharication Two commercial enzymes (Termamyl and Dextrozyme GA) were used for saccharication of PSP roots. Termamyl is a thermostable (>90C) a-amylase enzyme, and Dextrozyme (>45C) is a fungal amyloglucosidase (glucoamylase) enzyme. The enzymes were procured from Arun and Co., Mumbai, India. SWEET POTATO HERBAL WINE S.K. PANDA ET AL. 2 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. Fermentation Process Sweet potato is a starchy crop that requires saccharicationby enzymatic treatment for conversion of starch into sugars for wine making. Saccharication Freshly harvested PSP roots were washed in tap water to remove any dust or debris, and adhering water was wiped out with a clean cloth. One kilogramof roots was de-skinned and homogenized with 1 L of tap water using a mixer- cum-grinder. The homogenized PSP roots were mixed with 0.2%Termamyl and incubated at 90Cfor 1 h for liquefaction. The liqueed mash was cooled down to 45C, and 1% Dex- trozyme GA was added to it with thorough mixing. The enzyme-treated mash was incubated for 48 h at 45C in a bio- logical oxygen demand incubator (Calton, Mumbai, India) for saccharication. After 48 h, the saccharied mash was further cooled down to ambient temperature (28 2C) and squeezed through a clean and sterilized cotton cloth to extract the juice (must). The must taken in 1 L Erlenmeyer conical asks was treated with sodium metabisulphite (SMS) (100 mg/mL) to inhibit undesirable microorganisms such as acetic acid bacteria, wild yeasts and moulds (Amerine and Ough 1980). The sugar content of the must (16 Brix measured by a hand refractome- ter, Sipcon, Jalandhar, India) was ameliorated to 20 Brix by addition of cane sugar. The ameliorated must was thoroughly blended with the crushed medicinal plant parts at desired quantity (Table 1) before fermentation. The must was inocu- lated with 24 h starter culture of S. cerevisiae (2% v/v), and TABLE 1. MEDICINAL HERBS AND PLANT PARTS AND QUANTITY USED IN WINE PREPARATION (JAIN 1983) Common English name Botanical name Family Part of the plant used Quantity (g/kg) Medicinal properties Ink nut Terminalia chebula Combretaceae Fruit 5 Antiasthmatic, antidysenteric, antiparalytic in piles Belliric myrobalan Terminalia belerica Combretaceae Fruit 5 Anthelmintic, antiseptic, astringent, expectorant, laxative, lithotriptic, rejuvenative Indian gooseberry Emblica ofcinalis Phyllantheae Fruit 5 Diuretic, laxative, carminative, stomachic, astringent, antidiarrheal, antihemorrhagic and antianemic Ginger Zingiber ofcinale Zingiberaceae Rhizome 3 Anti-dyspepsia and anticancer Bael Aegle marmelos Rutaceae Fruit 5 Anti-chronic diarrhea, antidysentery, antipeptic ulcers and laxative Bishops weed Carum copticum Apiaceae Fruit 3 Antispasmodic, antiseptic Holy basil Ocimum sanctum L. Lamiaceae Leaves 5 Anti-bronchial, antiasthmatic, antimalarial, antidysenteric, antiarthritis Night jasmine Nyctanthes arbortristis Oleaceae Leaves 2 Antisciatica and antiarthritic, antipyretic and ulcerogenic activity Malabar nut Justcia adhatoda Acanthaceae Leaves 2 Antispasmodic, bronchodilator and mucolytic agent in asthma Five-leaved chaste tree Vitex negundo Lamiaceae Leaves 2 Anti-inammatory, antipyretic, tranquilizer, bronchial smooth muscle relaxant, antiarthritic, anthelminthic and vermifuge Garlic Allium sativum Amaryllidaceae Fruit 3 Hypolipidemic, antithrombotic, antioxidant, anticancerous and antimicrobial effects Cardamom Elleteria cardamomum Zingiberaceae Fruit 2 Antiasthma, anesthetic in burning sensation, anti- cold and cough, protects from diseases of bladder and kidney Cinnamon Cinnamonum verum Lauraceae Fruit 2 Anti-atulence, anti-piles, anti-amenorrheal, antidiarrheal Indian aloe Aloe vera Xanthorrhoeaceae Leaves 2 Anesthetize tissues, acts against bacterial, fungal and viral growth, anti-inammatory, dilate capillaries, anticancer and enhance blood ow Belladonna Atropa belladona Solanaceae Root 2 Promoter for wound healing Asparagus Asparagus adscendens Liliaceae Root 2 Anti-spermatorrhea, anti-chronic leucorrhea, antidiarrhea, antidysenteric symptom Round leaf buchu Barosma betulina Rutaceae Leaves 2 Stimulates kidney, cleanses blood, antiseptic, acts as tonic and promotes sweating Neem Azadirachta indica Meliaceae Bark 0.5 Analgesic, alternative, curative of fever S.K. PANDA ET AL. SWEET POTATO HERBAL WINE 3 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. (NH 4 ) 2 SO 4 at 0.1% concentration was added as the nitrogen source. Fermentation was carried out at ambient temperature (28 2C) for 5 days. Three replicates were maintained for conducting this experiment, andthe experiment was repeated twice. The data given were the mean of the two experiments. Racking and Bottling After fermentation, rst racking (decanting the top uid portionfromthe residue, settledat bottom) was carriedout at ambient temperature. Racking of wine was carried out when total soluble sugar (TSS) reached around 23 Brix. This process was repeated three times at 20 days interval to discard the settled residues. Bentonite (0.04%) was added before the nal racking to remove the last remaining residues. After nal racking, SMS (100 mg/mL) was added as preservative before bottling. The bottles were lled full with wine, corked and sealed with beeswax. The ow chart for making sweet potato herbal wine is shown in Fig. 1. Biochemical Analysis The biochemical compositions of the wine were determined as follows. Total titratable acidity (TA), tannin, lactic acid (LA), anthocyanin and ethanol contents of must and wine were determined by using the methods described by Amerine and Ough (1980). Starch and TS were quantied in the must and wine by anthrone method and reducing sugar was esti- mated by Nelsons method. (Mahadevan and Sridhar 1993). Total phenolic content was quantied by FolinCiocalteu method(Bray andThorpe 1954). The pHwas measuredusing a pH meter (Systronics Ltd., Ahmedabad, India), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of wine was measured by spectrophotometric method as described by Marxen et al. (2007). Preparation of Samples for FT-IR Analysis Herbal PSP wine and a commercial grape wine (Port wine 1000, Nashik Vintners Pvt. Ltd., Maharashtra, India) were subjected to FT-IRanalysis for the evaluation of the composi- tional difference. Two milliliters of the respective samples were applied on oval glass (having a diameter of 1 1 cm) and allowed to air dry overnight and then subjected to FT-IR for recording the spectra. The spectra were obtained with a Perkin-Elmer 2000 spectrometer (Perkin-Elmer, Norwalk, CT) at 4/cm resolution with a narrow band liquid N 2 -cooled mercury cadmium telluride detector. Statistical Methods The analytical variables of herbal PSP wine were subjected to PCA to group the related variables into smaller components for interpretation of their relationship. All analyses were per- formed using statistical software SPSS (SPSS Software for Windows release 17.0, SPSS Inc., Chicago, IL). RESULTS AND DISCUSSION In our previous study, we have developed a red wine from PSP variety ST-13 having the following proximate composi- tions: ethanol, 9.33% v/v; TA, 1.34 mg/100 mL; total phenol content (TPC), 0.36 g/100 mL; LA, 1.14 mg/100 mL; and DPPH scavenging activity, 58.95% (Ray et al. 2011). The wine was prepared by saccharication of PSP roots by com- bined treatment of a thermostable a-amylase (Termamyl) Purple sweet potato Liquefaction of pulp was done by adding Termamyl (0.2%) and incubated at 90C for 1h Washing, de-skinning and crushing with tap water Saccharification of pulp was done by adding Dextrozyme G.A. (1%) and incubated at 45C for Pressing and juice extraction Amelioration of must a. Adjustment of sugar to 20 Brix with cane sugar b. Addition of SMS (100g/ml) c. Addition of grinded medicinal plant parts Inoculation with 24h starter culture (2%) along with 0.1% (NH 4 ) 2 SO 4 as nitrogen source Fermentation at 282C for 5 days Racking First racking carried out at 3-4Brix. Racking was repeated for 3 times in 20 days interval Clarification with 0.04% bentonite before final racking Bottling and corking 100g/ml SMS was added Bottle was filled full, corking was done and the bottle was sealed with bee wax Herbal PSP wine FIG. 1. A FLOW CHART FOR MAKING HERBAL PSP WINE SWEET POTATO HERBAL WINE S.K. PANDA ET AL. 4 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. and an amyloglucosidase (Dextrozyme GA) and subsequent fermentation of the must using S. cerevisiae as the starter culture. In the present study also, PSP roots were saccharied by the combination of Termamyl and Dextrozyme GA, and in the process, there was 97% conversion of starch into sugars. From the initial content of 178 g/kg starch in the roots, the TS obtained after saccharication was 189.92 g/kg roots. The must obtained after saccharication was mixed with medicinal plant parts and fermented to prepare the herbal PSP wine (Fig. 2). The composition of the herbal PSP wine is presented in Table 2. The wine had a pHof 3.34, TAof 1.25 g tartaric acid/100 mL and LA of 1.92 mg/100 mL. Similar results were obtained with wines prepared from Indian gooseberry (E. ofcinalis) (Soni et al. 2009), herbal wine from infused tea leaves (Aroyeun et al. 2005) and other tropical fruits such as litchi (Litchi cinensis Sonn.) (Kumar et al. 2008), mango (Mangifera indica) (Reddy and Reddy 2005), jamun (Syzygium cumini L.) (Choudhury and Ray 2007), etc. The carbohydrate content of the wine was low (starch, 0.24 g/100 mL; TS, 0.95 g/100 mL; and TSS, 4.0 Brix). TPC of the wine was 0.19 g/100 mL, which is appar- ently equal to those reported for red wines (Sanchez-Moreno et al. 1998; Piaxao et al. 2007). The polyphenol content of a tea wine after 6-months maturation varied from 0.0013 g/ 100 mL to 0.0091 g/100 mL depending upon the clone type (Aroyeun et al. 2005). In our study, total phenolic content (0.19 mg/100 mL) of the herbal PSP wine might be the cumulative polyphenol contents of PSP and herbal additives. Recent epidemiological studies have shown that avonoid- rich foods and beverages, such as red wine, have benecial effects on cardiogenesis and cardiovascular diseases (Soleas et al. 1997). Further the lower pH (3.34) of the wine is highly favorable for the stability of the polyphenols as they are known to auto-oxidize with increase in pH (Mochizuki et al. 2002). The anthocyanin content of the herbal PSP wine was estimated to be 59.90 mg/100 mL. PSP anthocyanins are natural color pigments and also work as antioxidants. DPPH is a stable synthetic that has been commonly used for the determination of antioxidant capacity in plant extracts. The DPPH scavenging activity was 51.35% in wine at a dose of 250 mg/mL. The wine had a moderate ethanol concentration (8.61% v/v) similar to that of wine produced from infused tea leaves (Aroyeun et al. 2005). Certain biological active compounds are present in the herbal additives such as nimbi- din, margolone and margolonone in neem (Azadirachta indica) (Biswas et al. 2002), latex (activity against Corynebac- terium, Salmonella, Streptococcus, S. aureus) in aloe vera (Cowan 1999), allicin and ajoene in garlic (Allium sativum), atropine in belladonna (Atropa belladona), xanthotoxol, imperatorin, alloimperatorin and alkaloids like aegeline and marmeline in bael (Aegle marmelos) (Joy et al. 1998). Indian gooseberry (E. ofcinalis) is a rich source of natural vitamin C and contains cytokinin-like substances namely zeatin, zeatin riboside and zeatin nucleotide (Joy et al. 1998). The basic cardamom (Elleteria cardamomum) aroma is produced by a combination of the major components, oc-terpinyl acetate and 1,8-cineole. Eugenol (l-hydroxy-2- methoxy-4-allylbenzene), the active constituent present in holy basil (Ocimum sanctum L.) has been found to be largely responsible for the therapeutic values (Prakash and Gupta 2005). Medicinal foods and beverages are enriched in various nutrients and phytochemicals, all of which are supposed to confer specic health benets. These chemical compounds of the medicinal plants described previously, presumably present in the herbal PSP wine, may contribute many health benets. FIG. 2. HERBAL PSP WINE FROM SWEET POTATO TABLE 2. COMPOSITION OF HERBAL PSP WINE AND STANDARD DEVIATIONS Parameter Wine (mean value) Standard deviation TSS (Brix) 4.00 0.44 Reducing sugar (g/100 mL) 0.38 0.12 Starch (g/100 mL) 0.24 0.06 Total sugar (g/100 mL) 0.95 0.07 Titratable acidity (g tartaric acid/100 mL) 1.25 0.04 pH 3.34 0.12 Phenol (caffeic acid equivalent) (g/100 mL) 0.19 0.02 Anthocyanin (mg/100 mL) 59.90 1.30 Lactic acid (mg/100 mL) 1.92 0.32 Ethanol (% v/v) 8.61 1.88 DPPH (%) 51.35 0.19 PSP, purple sweet potato; TSS, total soluble sugar; DPPH, 2, 2-diphenyl-1- picrylhydrazyl. S.K. PANDA ET AL. SWEET POTATO HERBAL WINE 5 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. FT-IR Analysis FT-IR spectroscopy is a promising technique to provide information on antioxidant potential of red wine (Versari et al. 2010). InFT-IR, the wave numbers selected by the math- ematical algorithm are associated with the absorption bands of the most important compounds in wine. In the mid FT-IR region, the main ranges selected (3,7003,000/cm, 1,700 1,400/cmand 1,2001,000/cm) correspond to the absorption bands of OH, C = O and CC/CO stretching vibration (Colthup et al. 1990). Samples of herbal PSP wine and a commercial grape wine were subjected to FT-IR analysis for detection and compari- son of functional groups (Fig. 3). The spectra (05,000/cm) were interpreted by using the guidelines of Stuart (2004). The strong phenol OH stretch detected at 3,591/cm was indica- tive of the presence of alcohol and phenol groups in both herbal and commercial wine. Similarly, peaks detected at 1,843/cm showed the presence of anhydrides group (C = O stretching) in both the samples. Azo compound (n = Nstretching) was detected at 1,495/cm, second overtone CH stretching was detected at 1,179/cm and alkynes (=CH bending) was detected at 773/cm in both the wine samples. Further, the CN stretching bands showed the presence of amines. Aromatic amines showed bands in the range of 1,3231,250/cmandaliphatic amines showedbands at 1,220 1,000/cm in both the samples. Primary (NH 2 ), secondary (NH) and tertiary (no hydrogen attached to N) amines may be differentiated by using the infrared spectra. Some addi- tional new bands were detected in case of herbal-enriched PSP wine samples such as at C = O stretch bands at 1,710/cm and aliphatic CNstretching at 1,269/cm. Asimilar study was conducted by Versari et al. (2010) to predict the total antioxi- dant capacity of red wine using FT-IR spectra in a range of 6004,400/cm. The result revealed a strong correlation betweenphenolic content and the total antioxidant activity of red wines (Versari et al. 2010). PCA of the Analytical and Proximate Data of PSP Wine The correlation analyses among the proximate and analytical variables are presented in Table 3. The most important correlations were starch-TSS (-0.524), ethanol-reducing sugar (-0.556), LA-anthocyanin (0.625), DPPH-anthocyanin (0.549), DPPH-LA(0.815). Using PCA, the 11 original proxi- mate and analytical variables were reduced to four principal components (PC1PC4), which had eigenvalues larger than 1 and retained for rotation (Hair et al. 1998). PC1, PC2, PC3 and PC4 accounted for 24.18%, 20.18%, 15.41%and 14.76%, respectively, and the total variations (74.53%) are presented in Table 4. FIG. 3. INFRARED SPECTRA OF HERBAL PSP WINE AND A COMMERCIAL (GRAPE) WINE SWEET POTATO HERBAL WINE S.K. PANDA ET AL. 6 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. To assist the interpretation of dimensions, the factor pattern was rotated using the varimax method. Based on the guidelines provided by Stevens (1992), anattribute was corre- lated to load heavily on a given component in the factor loading was greater than 0.72. All the 11 analytical variables loaded heavily on the dimension. Three analytical variables i.e., TS (+ve), anthocyanin (+ve), DPPH (+ve) and LA (+ve) were loaded heavily on PC1. TSS (-ve) and starch (+ve) have been loaded substantially on PC2. Reducing sugar (+ve) and TA (+ve) were loaded on PC3 and PC4, respectively. Plotting PC1 loading against PC2 loading depicted these relationships visually (Fig. 4). Several studies have been conducted by using PCA for the evaluation of fermented food products. Panda et al. (2009) have demonstrated the application of PCAin the production, nutritional and proximate composition of anthocyanin-rich sweet potato pickle. PCA reduced the 11 original analytical and proximate variables (pH, TA, LA, starch, TS, anthocya- nin, organic matter, ash, fat, protein and calories) into three independent components (factors), whichaccountedfor 91% of the total variations. PCA was applied for evaluation of proximate compositions of litchi wine, inwhicheight original proximate variables were reduced to two independent com- ponents (PC1 and PC2) that accounted for 100% variations inlitchi wine. Inanother study, PCAreducedthe six analytical variables of LA fermented cabbage juices to two independent TABLE 3. CORRELATION COEFFICIENT FOR ANALYTICAL + PROXIMATE VARIABLE OF HERBAL PSP WINE TSS Reducing sugar Starch Total sugar Titratable acidity pH Phenol Anthocyanin Lactic acid Ethanol DPPH 1.000 -0.112 -0.524 0.187 -0.0293 -0.386 0.037 0.000 -0.341 0.333 -0.398 1.000 0.048 0.071 0.256 -0.027 -0.048 -0.049 0.193 -0.556* 0.046 1.000 0.214 0.209 0.273 -0.071 0.099 0.386 0.026 0.414 1.000 0.163 -0.055 -0.357 0.247 0.410 0.159 0.184 1.000 0.133 -0.116 0.241 0.094 0.026 0.090 1.000 -0.055 0.255 0.248 -0.030 0.316 1.000 -0.247 -0.337 0.000 -0.092 1.000 0.625* 0.220 0.549* 1.000 -0.107 0.815 1.000 -0.102 1.000 * Correlation is signicant at the 0.05 level (two-tailed). Correlation is signicant at the 0.01 level (two-tailed). PSP, purple sweet potato; TSS, total soluble sugar; DPPH, 2, 2-diphenyl-1-picrylhydrazyl. TABLE 4. RESULTS OF PRINCIPAL COMPONENTS ANALYSIS OF WINE PROXIMATE VARIABLES OF HERBAL PSP WINE Parameter PC1 PC2 PC3 PC4 TSS 0.158 -0.909 -0.137 -0.145 Reducing sugar 0.118 -0.006 0.949 0.198 Starch 0.258 0.721 0.113 -0.036 Total sugar 0.727 -0.391 -0.295 0.176 Titrable acidity 0.080 0.057 0.324 0.827 pH 0.101 0.579 -0.052 0.227 Phenol -0.156 -0.194 0.168 -0.685 Anthocyanin 0.747 0.096 0.008 0.373 Lactic acid 0.872 0.089 0.432 0.134 Ethanol 0.048 -0.436 -0.593 0.359 DPPH 0.831 0.443 0.033 -0.202 Variance explained (%) 24.18 20.18 15.41 14.76 Eigenvalue 3.28 2.26 1.38 1.33 Extraction method: principal component analysis. Rotation method: varimax with kaiser normalization (eigenvalue). PSP, purple sweet potato; TSS, total soluble sugar; DPPH, 2,2-diphenyl-1-picrylhydrazyl. -0.50 0.00 0.50 Principal Component 2 (20.18%) 0.00 0.20 0.40 0.60 0.80 P r i n c i p a l
C o m p o n e n t
1
( 2 4 . 1 8 % ) TSS RS Starch TS TA pH Phenol Anthocya LA Ethanol DPPH FIG. 4. GRAPHICAL REPRESENTATION OF PRINCIPAL COMPONENTS PC1 VERSUS PC2 OF ANALYTICAL VARIABLES FROM HERBAL PSP WINE S.K. PANDA ET AL. SWEET POTATO HERBAL WINE 7 Journal of Food Processing and Preservation (2012) 2012 Wiley Periodicals, Inc. components, which accounted for 87.10% of the total vari- ance (PC1, 63.80% and PC2, 23.30%) (Karovicova et al. 2001). CONCLUSIONS Herbal-enriched PSP wine, developed from medicinal herbal parts and PSP is a unique and novel product, which presum- ably behold remedies for common ailments like cold, cough, chronic dysentery, diabetes, skin diseases, etc.. The wine has a higher anthocyanin (59.90 mg/mL) content and higher free radical scavenging activity. The correlation between DPPH and anthocyanin depicts the health benets of the herbal- enriched PSP wine. The wine is moderately alcoholic (8.61%) and has a medicinal avor. The dark color of the wine is attractive. 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