BIO 4V40

Medical and Molecular Cell Biology

Fall 2005

Class Times: Tues. and Thurs. 12:30- 1:45; FO 3.616

Instructor: Dr. Gail Breen

FO 3.710; (972) 883-2504
Office Hours: Tues. 2 pm (or by appointment)

Course Description:

This course will focus on the molecular and cellular basis of various processes involved in health and disease.
The goals of this course are:
1) Development of skills needed to critically read and understand scientific literature.
2) Development of skills necessary for clear, precise and accurate oral presentation of scientific data.

Course Format:

For the first part of this course, selected papers or book chapters will be assigned for each class or topic. Students are
expected to have read the assigned papers or chapters before class and be prepared to discuss and analyze them in
class. Attendance and class participation are an important part of this class. There will also be two examinations
covering this material.

For the second part of this course, students will present a seminar (approximately 30 minutes in length) on an approved
topic of their choice. Students should post their primary paper(s) at least one week in advance of their presentation.

Course Grading: Attendance and Class Participation 25%

Oral Presentation 35%
Exams 40%

Prerequisites: BIO 3302 (Eukaryotic Cell and Molecular Biology)

BIO 3380 (Biochemistry or Cell Biology Laboratory)
BIO 3161 and 3162 (Biochemistry)
 SCHEDULE 2

Aug. 18 Introduction

Aug. 23 Mitochondrial DNA and Genetics

Lodish et al., Molecular Cell Biology, Chapter 10, pp 438- 443

www.mitomap.org

Aug. 25 Mitochondrial Diseases

www.mitomap.org

The mitochondrial genome in human adaptive radiation and disease: On the road to therapeutics
and performance enhancement (review)
Gene 2005 Jul 14; [Epub ahead of print]
Wallace DC

Mitochondrial DNA Mutations in Human Disease (review)

Nature Reviews (2005) 6: 389-402
Taylor RW and Turnbull DM

Aug. 30 Mitochondrial Diseases

Mitochondrial disorders (review).

Brain 2004; 127 (Pt 10): 2153-72.
Zeviani M, Di Donato S.

Sept. 1 Mitochondrial Diseases

Identification of a gene causing human cytochrome c oxidase deficiency by integrative

genomics.
Proc Natl Acad Sci U S A. 2003 Jan 21; 100(2):605-10.
Mootha VK, Lepage P, Miller K, Bunkenborg J, Reich M, Hjerrild M, Delmonte T, Villeneuve
A, Sladek R, Xu F, Mitchell GA , Morin C, Mann M, Hudson TJ, Robinson B, Rioux JD, Lander
ES.

Sept. 6 Mitochondria and Aging

Mitochondria, oxidants, and aging (review article)

Cell 2005 Feb 25; 120(4): 483-95.
Balaban RS, Nemoto S, Finkel T.
Sept. 8 Mitochondria and Aging

Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging.

Science 2005 Jul 15; 309 (5733): 481-4.
Kujoth GC, Hiona A, Pugh TD, Someya S, Panzer K, Wohlgemuth SE, Hofer T, Seo AY,
Sullivan R, Jobling WA, Morrow JD, Van Remmen H, Sedivy JM, Yamasoba T, Tanokura M,
Weindruch R, Leeuwenburgh C, Prolla TA.

Sept. 13 Mitochondria and Aging

Extension of murine life span by overexpression of catalase targeted to mitochondria.
Science 2005 Jun 24; 308(5730): 1909-11
Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges
W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS.

Sept. 15 NSF meeting at UTD- no class

Sept. 20 Exam 1

Sept. 22 Proteomics of Mitochondria

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse
mitochondria.
Cell 2003 Nov 26; 115(5):629-40.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR , Stahl E, Bolouri MS, Ray
HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M.

Sept. 27 Mitochondria and Alzheimer's Disease

ABAD Directly Links Aß to Mitochondrial Toxicity in Alzheimer's Disease

Science (2004) 304: 448-452
Lustbader JW, Cirilli M, Lin C, Xu HW, Takuma K, Wang N, Caspersen C, Chen X, Pollak S,
Chaney M, Trinchese F, Liu S, Gunn-Moore F, Lue LF, Walker DG, Kuppusamy P, Zewier ZL,
Arancio O, Stern D, Yan SS, Wu H

Mitochondrial dysfunction and Alzheimer's disease: role of amyloid-beta peptide alcohol

dehydrogenase (ABAD) (review)
Int J Exp Pathol. 2005 Jun; 86(3):161-71.
Yan SD, Stern DM.

Sept. 29 Mitochondria and Alzheimer's Disease

Alzheimer's brains harbor somatic mtDNA control-region mutations that suppress mitochondrial
transcription and replication.
Proc Natl Acad Sci U S A. 2004 Jul 20; 101(29):10726-31.
Coskun PE, Beal MF, Wallace DC

Oct. 4 Mitochondria and Diabetes

PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately

downregulated in human diabetes.
Nat Genet. 2003 Jul; 34(3):267-73.
Mootha VK, Lindgren CM, Eriksson KF , Subramanian A, Sihag S, Lehar J, Puigserver P,
Carlsson E, Ridderstrale M, Laurila E, Houstis N, Daly MJ, Patterson N, Mesirov JP, Golub TR,
Tamayo P, Spiegelman B, Lander ES, Hirschhorn JN, Altshuler D, Groop LC
Oct. 6 Mitochondria and Cancer

mtDNA mutations increase tumorigenicity in prostate cancer.

Proc Natl Acad Sci U S A. 2005 Jan 18; 102(3):719-24.
Petros JA, Baumann AK, Ruiz-Pesini E, Amin MB, Sun CQ, Hall J, Lim S, Issa MM, Flanders
WD, Hosseini SH, Marshall FF, Wallace DC .

Oct. 11 Regulation of Mitochondrial Biogenesis

Myc stimulates nuclearly encoded mitochondrial genes and mitochondrial biogenesis

Mol Cell Biol. 2005 Jul; 25(14):6225-34.
Li F, Wang Y, Zeller KI, Potter JJ, Wonsey DR, O'Donnell KA, Kim JW, Yustein JT, Lee LA,
Dang CV

Oct. 13 TBA

Oct. 18 Exam II

Oct. 20 Genetics of Alzheimer's Disease Meeting, No class

Oct. 25 Student Presentation- Morrison

Oct. 27 Student Presentation- Lee, Sang Won

Nov. 3 Student Presentation-McNeill

Nov. 8 Student Presentation-Shin

Nov. 10 Student Presentation-Ali

Nov. 15 Student Presentation-Tata

Nov. 17 Student Presentations- Patel and Kiamanesh

Nov. 22 Last Class, Student Presentations- Singh and Denison