Professional Documents
Culture Documents
Sc BIO-TECHN OLOGY
2012-13 ON WARD S
S.
No. Course No.
1
2
3
4
5
6
7
BT-501 MS
Course Title
Teaching
Examination Scheme
Duration
Scheme
Credit of Exam
L
P Total Sessional External Marks Total
(Hours)
Marks
Theory Practical
4
50
100
150
4
4
4
4
4
4
50
50
50
100
100
100
150
150
150
4
4
4
3
3
3
4
4
4
4
50
50
50
50
100
100
2
2
6
6
50
50
100
16
12
28
350
400
150
900
22
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
S.
No. Course No.
1
2
3
4
5
6
7
BT-502 MS
BT-504 MS
BT-506 MS
BT-508 MS
Course Title
BIOINSTRUMENTATION
IMMUNOLOGY-I
GENETIC ENGINEERING-I
INTRODUCTION TO
BIOINFORMATICS
BT- 510 MS BIOTECHNOLOGY LAB - IV
BT-512 MS BIOTECHNOLOGY LAB V
BT-514 MS BIOTECHNOLOGY LAB VI
TOTAL
Teaching
Examination Scheme
Duration
Scheme
Credit of Exam
L
P Total Sessional External Marks Total
(Hours)
Marks Theory Practical
4
4
4
4
4
4
4
4
50
50
50
50
100
100
100
100
150
150
150
150
4
4
4
4
3
3
3
3
16
4
4
4
12
4
4
4
28
50
50
50
350
400
50
50
50
150
100
100
100
900
2
2
2
22
6
6
6
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
Each student has to undergo a professional training of at least 4 w eeks from a
biotechnology-based industry/ research Institute/ laboratory. Its evaluation w ill be carried
out in the 3rd Semester.
S.
No. Course No.
1
2
3
4
5
6
7
8
Course Title
Teaching
Examination Scheme
Duration
Scheme
Credit of Exam
L
P Total Sessional External Marks Total
(Hours)
Marks
Theory Practical
4
50
100
150
50
100
150
50
100
150
50
100
150
50
50
100
50
50
100
50
50
100
50
50
16
14
30
400
400
150
950
24
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
S.
No. Course No.
1
2
3
4
5
6
7
8
Course Title
Teaching
Examination Scheme
Scheme
Duration
L
P Total Session External Marks Total Credit of Exam
(Hours)
al
Theory Practical
Marks
4
4
50
100
150
4
3
4
4
4
4
4
4
2
14
50
100
150
100
150
50
50
50
100
-
50
50
150
100
100
4
2
2
3
6
6
4
2
50
50
50
-
100
50
2
2
6
-
30
400
400
150
950
24
50
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
S.
No.
1
2
3
4
6
7
8
Course
No.
Course Title
Teaching
Examination Scheme
Duration
Scheme
Credit of Exam
L P Total Sessional External Marks Total
(Hours)
Marks
Theory Practical
BT-602 MS MEDICAL
4
BIOTECHNOLOGY
BT-604 MS ANIMAL BIOTECHNOLOGY- 4
I
BT-606 MS ENVIRONMENT
4
BIOTECHNOLOGY-I
BT-608 MS BIOETHICS, BIOSAFETY
4
AND IPR
BT-610 MS BIOTECHNOLOGY LAB X
BT-612 MS BIOTECHNOLOGY LAB - XI
BT-614 MS SEMINAR
TOTAL
16
50
100
150
50
100
150
50
100
150
50
100
150
4
4
2
10
4
4
2
26
50
50
50
350
400
50
50
100
100
100
50
850
2
2
2
22
6
6
-
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
S.
No.
1
2
3
4
6
7
8
Course
No.
Course Title
Teaching
Examination Scheme
Duration
Scheme
Credit of Exam
L P Total Sessional External Marks Total
(Hours)
Marks
Theory Practical
BT-602 MS MEDICAL
4
BIOTECHNOLOGY
BT-604 MS ANIMAL BIOTECHNOLOGY- 4
I
BT-606 MS ENVIRONMENT
4
BIOTECHNOLOGY-I
BT- 608
BIOETHICS, BIOSAFETY
4
MS
AND IPR
BT-610 MS BIOTECHNOLOGY LAB X
BT-612 MS BIOTECHNOLOGY LAB - XI
BT-614 MS SEMINAR
TOTAL
16
50
100
150
50
100
150
50
100
150
50
100
150
4
4
2
10
4
4
2
26
50
50
50
350
400
50
50
100
100
100
50
850
2
2
2
22
6
6
-
Note:
The students w ill be allow ed to use non-programmable scientific calculator. How ever,
sharing/exchange of calculator is prohibited in the examination.
Electronic gadgets, including cellular phones are not allowed in the examination.
BT 501 MS
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
Basic Stru cture and fu nction of cells and their organelles: Cellular and su bcellu ar
d ifferences betw een prokaryotes and eu karyotes, stru ctu re and fu nctions of cellu lar
organelles.
Transport across biom em branes: Transport of nu trients, ions and m acrom olecules
across membranes, facilitated transport, group translocation, active transport.
Protein sorting and vesicu lar trafficking: Transp ort of m olecules into and ou t of the
nu cleu s, transport of proteins into m itochond ria and chloroplasts, transport from the
ER through golgi apparatus to lysosomes.
UNIT-II:
Cell-Cell Interactions: General principles of cell to cell com m u nication, cell ad hesion
and role of different adhesion molecules, extracellular matrix.
Cell cycle and Regulation: Molecu lar events and m od el system s, m echanics of cell
division, regulation of cell cycle.
Cell Signaling: Signalling via G- Protein- linked cell su rface receptors and via
enzyme- linked cell surface receptors, two component signalling.
UNIT-III:
Basic concepts of genetic inform ation: DN A and RN A as genetic m aterial, chem ical
stru ctu re and base com position of nu cleic acid s, su percoiling of DN A, DN A
reassociation kinetics (cot cu rve analysis), DN A organization into chrom atin,
bacterial and eukaryotic genomic organization.
Genetic Material: replication and repair: enzym es and p roteins of DN A replication,
prokaryotic and eu karyotic replication m echanism , replication in phages and
retroviruses, DNA repair mechanisms.
UNIT-IV:
BT 503 MS
BIOCHEMISTRY
M. Sc Semester - I (Biotechnology)
L
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
UNIT-I:
of
Proteins Classification based on stru ctu re and fu nction, forces stabilizing protein
structure and shape, different levels of structural organization.
Carbohydrates Stru ctu re and properties of m onosaccharid es, oligosaccharid es and
polysaccharides, and their biological functions.
UNIT-II:
Lipids Classification sim ple and com plex lipid s, fatty acid s stru ctu re and
nom enclature, soap valu e, acid value, iod ine num ber, rancid ity, stru ctu re and
function of triacylglycerols, phospholipids, glycolipids and steroids.
N ucleic acids: Physical & chem ical properties of nu cleic acid s, stru ctu re and
properties of pu rine & pyrim id ine bases, nu cleosid es & nu cleotid es, biological
fu nctions of DN A & RN A species, d ouble helical m od el of DN A stru ctu re and forces
responsible for it , A, B, & Z DNA.
UNIT-III:
UNIT-IV:
Protein Metabolism : Essential & non essential am ino acid s, d egrad ation &
biosynthesis of am ino acid s, u rea cycle: reactions, regu lation and its linkage w ith
the citric acid cycle, nitrogen cycle, regulation of amino acid bio synthesis.
N ucleic Acid Metabolism: De-novo biosynthesis of pu rine and pyrimidines,
regulation of p u rine & pyrim id ine biosynthesis, salvage pathw ay, form ation of
deoxyribonucleotides, catabolism of purines & pyrimidines.
BT 505 MS MICROBIOLOGY-I
M. Sc. Semester - II (Biotechnology & Bioinformatics)
L
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
UNIT-II:
UNIT-III:
Microbial N u trition and Grow th: Grow th requirem ents, culture m ed ium , axenic
cu ltu res, isolation and preservation of pu re cu ltures, grow th cu rve, m easu rem ent of
m icrobial grow th, batch and continu ous cu ltu re, chem ostat and turbid ostat, influ ence
of environm ental factors on grow th, bacterial rep rod u ction- transformation,
transduction and conjugation.
Control of Microorganism s: Sterilization, d isinfection, factors influencing
effectiveness of antimicrobial agents, physical and chemical methods of control.
UNIT-IV:
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at
least one from each unit. All questions will carry equal marks.
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
UNIT-II:
Sim ple linear regression and correlation. Introd u ction to d igital com pu ters:
organizations; low level and high-level languages, the binary num ber system . Flow
charts and programming techniques.
UNIT-III:
Introd u ction to program m ing in Q basic and C. Introd u ction to d ata stru ctures and
d atabase concepts, introd u ction to internet and its applications. Introd u ction to MSOffice softw are, covering w ord processing, spread sheets and presentation softw are.
Introduction to Harvard graphics/Sigma plotter.
UNIT-IV:
Com pu ter oriented statistical techniques: Frequ ency table of single d iscrete variable,
bubble sort, computation of mean, variance and standard deviation; t-test, correlation
coefficient. Bioinformatics and biotechnology.
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at least
one from each unit. All questions will carry equal marks.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
H istology of variou s organ system s (N ervous, Digestion, Rep rod u ctive, Respiratory, and
Circulatory system).
4.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
2.
3.
4.
5.
6.
7.
8.
9.
10. Analysis of blood / urine samples for sugars / urea / bile pigments.
11. Assay of enzyme activities alkaline phosphatase /peroxidase /oxidase
12. Gel electrophoresis of DNA /RNA.
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
2.
3.
4.
Preparation of m ed ia for grow th of variou s m icrobes; cu ltu ring and enum eration of
microbes.
5.
6.
7.
8.
9.
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
BT 502 MS
BIOINSTRUMENTATION
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT I:
Microscopy: Light, electron (scanning & transm ission), phase contrast, flu rosence
icroscopy, freeze fractu re techniques, specific staining of organelles or m arker
enzymes.
Ultracentrifugation: Sed im entation of m acrom olecu les, centrifu gation techniqu es and
their applications, d ifferential centrifu gation, zonal, d ensity grad ient and
ultracentrifugation techniques.
UNIT II:
UNIT III:
UNIT IV :
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at least
one from each unit. All questions will carry equal marks.
BT 504 MS IMMUNOLOGY-I
M. Sc. Semester - II (Biotechnology)
L
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
UNIT-I:
of
The Components of Immunity: Innate and Acquired Immunity. Cells and organs
of immune System, phagocytosis, and inflammatory responses, hematopoesis.
Immunogens and Immunoglobulins: Antigens, haptens, su perantigens,
antigenic d eterm inants, im m u noglobu lins- basic stru ctu re, classes and
su bclasses of im m u noglobu lins, biological effector fu nctions m ed iated by
antibod y, genetic organization of im m u noglobulin genes, generation of
antibody diversity.
UNIT-II:
UNIT-III:
UNIT-IV:
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting
tw o from each unit. The candidates w ill be required to attempt five questions in all
selecting at least one from each unit. All questions will carry equal marks.
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
Duration of Examination
3 Hours
UNIT-I:
Tools for Gene Cloning: Introd u ction, historical backgrou nd , restriction enzym es,
m od ifying enzym es, ligases, polym erases, linkers and ad aptors, hom opolym eric
tailing, labelling of DNA, nick translation, random priming
Cloning vectors: plasm id s, bacteriophages, cosm id s and phasm id s, artificial
chromosomes as vectors (YAC, BAC, MAC).
UNIT-II:
Cloning strategies and recom binant selection: Cloning strategy, m ethod s of gene
isolation, Chem ical Synthesis of gene, construction of genom ic and cDN A library.
Chrom osom e w alking, chrom osom e jum ping, d ifferent screening m ethod s: genetic,
immunochemical, nucleic acid hybridization, HRT and HART.
UNIT-III:
Molecu lar probes and Polym erase Chain Reaction: Molecu lar probes, labeling
(rad ioactive vs non-rad ioactive) and u ses of p robes, prim er d esigning, PCR:
principle, typ es; m od ified PCR (inverse PCR, anchored PCR, PCR for m u tagenesis,
RT-PCR, asym m etric PCR), cloning of PCR p rod u cts. Applications of PCR.
Expression of cloned genes: In bacteria, in yeast, expression in p lants and in
mammalian cells.
UNIT-IV:
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
Introd u ction to operating and num ber system s:Wind ow s, Unix, Linu x; binary, octal
and hexadecimal number systems.
Basics of N etw orking and program m ing: Internet, Intranet, netw orking protocols,
FTP,www., f l o w ch a r t s , a l g o r i t h m s , a s s e m b l e r s , i n t e r p r e t e r s , c o m p i l e r s .
Databases and Databanks: Introd u ction, collecting and storing sequences in
laboratory, stru ctu re d atabases: PDB, MMDB, NCBI d atabases: SEQ-IDs, Bio SEQsSETs, SEQ- ANNOT, SEQ- DESCR, submitting DNA sequences to the databases.
UNIT-II:
I n fo r m a t i o n r e t r i e v a l fr o m b i o l o g i ca l d a t a b a s e s : R e t r i e v i n g d a t a b a s e
e n t r i e s , i n t e g r a t e d i n fo r m a t i o n r e t r i e v a l , EN T R EZ s y s t e m , s e q u e n ce
d a t a b a s e s b e y o n d N C BI, m e d i ca l d a t a b a s e s .
Sequ ence alignm ent and d atabase searching: Introd u ction, evolu tionary basis of
sequ ence alignm ent, optim al alignm ent m ethod s, statistical significance of alignm ent,
database similarity searching, FASTA, BLAST.
Mu ltiple sequ ence alignm ent: Exhaustive algorithm s, heu ristic algorithm s,
progressive alignment methods, iterative alignment, block-based alignment.
UNIT-III:
UNIT-IV:
Pred ictive m ethod s using nu cleotid e sequ ences: fram ew ork, m arking repetitive
DN A, d atabase search, cod on bias d etection, d etecting fu nction sites in the DM,
integrated gene passing, finding tRNA genes.
Pred ictive m ethod s u sing protein sequ ences: p r o t e i n i d e n t i t y b a s e d o n
c o m p o s i t i o n , P r o p s e a r ch , p h y s i ca l p r o p e r t i e s b a s e d o n s e q u e n ce s ,
s e c o n d a r y s t r u c t u r e a n d fo l d i n g cl a s s e s , Ss p r e a d So p m a , s p e ci a l i z e d
s t r u ct u r e o f fe a t u r e s , t e r t i a r y s t r u ct u r e .
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
List of Experiments/Exercises
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
Routine techniques in handling laboratory animals: Feeding, cleaning & hygienic measures.
Inoculation and bleeding of laboratory animals (mice/ rabbit).
Preparation and administration of antigens.
Isolation and purification of immunoglobulins.
Quantification of immunoglobulins.
To perform single radial immunodiffusion test.
To perform double immunodiffusion test.
Latex agglutination test.
To perform different types of ELISA.
To perform immunoeletrophoresis with the given antigen-antibody sample.
To perform differential leukocyte count of the given blood sample.
To perform total leukocyte count of the given blood sample.
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
List of Experiments/Exercises
1.
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
6 Hours
D uration
Examination
of
List of Experiments/Exercises
1.
2.
databases.
Database file formats and Data retrieval tools: SRS, Entrez.
3. Similarity searching using NCBI, BLAST and multiple sequence alignment using Clustal.
4.
5.
6.
7.
8.
PERL Programming.
9. To tabu late statistical inform ation given in d escriptive form and u se graphical techniqu es
to interpret it.
10. To compute the correlation coefficient for bivariate data and interpret it.
TEXT /REFERENCE BOOKS:
1. Bioinformatics: A Practical Approach by K. Mani and N. Vijayaraj, Aparna Publications,
Coimbatore.
2. Bioinformatics. Higgins & Taylor (2000). OUP.
3. Practical statistics for Experimental Biologists. Wardlaw, A.C. (1985). John Wiley and
Sons.
4. Programming Perl Larry Wall, Tom Christiansen & John Orwant 3ed 2000- OReilly
5. Programming Perl - Tom Christiansen, Larry. Wall Orielly Publications
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
UNIT-II:
Genom e sequ encing, analysis and annotation: DN A sequ encing- principle and
technique, pyrosequ encing; au tom ated , w hole genom e, and shotgu n sequencing,
clone-contig assem bly, hu m an genom e sequ encing project- a brief accou nt; analysis
of sequ ence d ata- experim ental and com pu tational approaches for d eterm ining gene
location and for fu nctional analysis of ind ivid u al genes; genom e sequ ence
annotation; com parative genom ics; transcriptom ics- stu d ying transcriptom e by
sequence analysis and microarray analysis, SAGE, parallel signature sequencing.
UNIT-III:
Proteom ics: Protein profiling- two-d im ensional separation of total cellular proteins,
isolation and sequ ence analysis of ind ivid ual protein spots, MALDI-TOF for protein
id entification, protein characterization by m ass spectrom etry, protein expression
analysis u sing m icroarrays; stru ctu ral analysis of proteins- X-ray crystallography and
N MR spectroscopy; protein-protein interactions- phage d isplay, yeast tw o-hybrid
system ; analysis of protein com plexes- affinity pu rification and m ass spectrom etry,
metabolomics and systems biology.
UNIT- IV:
Applications of genom ics and p roteom ics: in research, hu m an health care, and in
agricultu re, pharm acogenom ics, high throu ghpu t screening in genom e for d ru g
d iscovery and id entification of gene targets, com parative genom ics, fu tu re
perspectives and developments.
BT 603 MS
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I: Introd u ction to enzym e: H istory ,N om enclatu re and classification of enzym es; Isoenzym es,
enzym e specificity, m onom eric and oligom eric enzym es, m u ltienzym e com plex,
holoenzym e, apo-enzym e, Units of enzym e activity (d efinition of IU, Katal), sp ecific
activity of enzym e, m easurem ent of enzym e activity, enzym e tu rnover. Ribozym es and
abzymes A brief account.
UNIT-II: Enzym e kinetics : Enzym e kinetics and its im portance, Method s u sed for investigating the
kinetics of enzym e catalyzed reactions; factors affecting the velocity of enzym e catalysed
reaction; Michaelis-Menten equation, Vm ax, Km and its significance; Linew eaver Bu rk
plot- its ad vantages and lim itations, Ead ie- H ofstee and H anes plots; enzym e inhibition,
types of enzym e inhibitions- com petitive, u ncom p etitive, noncom petitive. Bisu bstrate
reactions- brief introduction to sequential and pingpong mechanism with examples.
UNIT-III: Strategies u sed for enzym e prod u ction, isolation and pu rification, m ethod of calculating the
pu rification fold ; estim ation of enzym e activity; characterization of an enzym e, criteria of
enzym e pu rity, d eterm ination of the m olecular w eight, enzym e im m obilization; protein
engineering, enzyme reactors.
UNIT-IV: Ind u strial uses of enzym es: Enzym e u sed in d etergents, u se of proteases in food , leather
and w ool ind u stries, prod u ction of glu cose syru p from starch u sing starch hyd rolyzing
enzym es, prod u ction of syru p containing m altose, enzym e in su crose ind ustry, glu cose
from cellu lose. Lactose in d airy ind u stry, glu cose oxid ase and catalase in food ind u stry and
medical application of enzymes. Basic principles of biosensors and their applications.
TEXT / REFERENCE BOOKS:
1.
2.
3.
4.
5.
6.
7.
8.
9.
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at least
one from each unit. All questions will carry equal marks.
Credits
Class Work
: 50 Marks
--
Examination
: 100 Marks
Total
D uration
Examination
: 150 Marks
of
: 3 Hours
UNIT I.
Ind u strial Microbiology: Introd u ction, objectives and applications. Cu ltivation &
Maintenance of Microorganism s: Different types of cu ltu re m ed ium ; C/ N / P
balance and d esign of cu ltu re m ed iu m . Su bstrates for ind ustrial m icrobial
processes, in d u s t r i a l l y i m p o r t a n t m i cr o b e s : isolation, preservation and
improvement o f i n d u s t r i a l l y i m p o r t a n t m i cr o o r g a n i s m s , s e l e ct i o n o f
m u t a n t s , a n d u se o f r e co m b in a n t D N A t e ch n o lo g y .
UNIT II.
Prod u ction and Process technology for m icrobial prod u cts: Microbial grow th
kinetics, Prim ary m etabolite: ethanol, citric acid , vinegar and am ino acid ,
prod u ction of alcoholic beverages-w ine and beer, m icrobial prod u ction of
industrial enzymes
UNIT III.
UNIT IV.
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
Duration of Examination
3 Hours
UNIT-I:
UNIT-II:
UNIT-III:
UNIT-IV:
Plant Cell and Tissu e Cu ltu re: Totipotency; Organogenesis; Som atic em bryogenesis;
Types of cu ltu re: seed , em bryo, callu s, organ, cell and protoplast cu lture , m eristem
and shoot tip culture, bud culture, organogenesis, embryogenesis
Micropropagation: Advantages and disadvantages of Micropropagation.
Cell Suspension cu ltu res: types of su spension cu ltu res: batch, continu ou s and sem icontinuous.
Cryopreservation as a Germ plasm storage m ethod , cryoprotectants, pre-treatment,
freezing, storage, thawing and its applications.
Protoplast isolation: Cu ltu re and usage; Som atic hybrid ization Protoplast fu sion:
methods, identification and selection of hybrid cells, Cybrids : Applications.
In vitro production of Haploids: androgenesis ,gynogenesis and their applications.
Gene transfer in plants: Plant gene stru ctu re as a d iscontinu ou s gene, Control
sequ ences Transient and stable gene expression, Marker genes, selectable and scorable
markers.
Direct gene transfer m ethod s - PEG-m ed iated , electroporation, particle bom bard m ent
and alternative m ethod s-Macroinjection, m icroinjection, liposom e m ed iated , silicon
carbide fiber mediated and chemical methods.
Ind irect gene transfer m ethod s, Genetic Transform ation w ith Agrobacterium ;Virulence;
Ti and Ri plasm id s; T-DN A transfer; Disarm ing the Ti plasm id . Agrobacteriummediated gene delivery; Cointegrate and binary vectors
Genetic m od ifications for crop im provem ent and Strategies for Introd u cing Biotic and
Abiotic Stress Resistance/ Tolerance: Bacterial resistance; Viral resistance; Fu ngal
resistance; Insects and pathogens resistance; H erbicid e resistance; transgenics for
flow er color and shape, transgenics for m ale sterility, qu ality im provem ent::
m od ification for higher carbohyd rate, protein and oil content; antisense gene approach,
Flavr Savr tom ato and Gold en rice (as case stu d ies); plant-d erived vaccines. Plants as
Biofactories: Concept of biofactories., com m ercial transgenic crops at global and
national level.
Texts/References:
1. H .S Chaw la: Introd u ction to Plant Biotechnology, 2004, Oxford and IBH Pu blishers, N ew
Delhi,
2. Christou and Lee: Volume I and Volume II : Handbook of Plant Biotechnology,2002
3. Ad rian Slater, N igel Scott and Mark Fow ler, Plant Biotechnology: The genetic m anipulation
of plants, 2003: 1st Edition, Oxford University Press,
4. Chrispeels , MJ and Sad ava, DE, Plants, Genes and Crop Biotechnology, 2003: 2nd edition,
American Society of Plant Biologists, Jones and Bartlett Publishers, USA
5. Arie Altman, Marcel Dekker, Inc. 2001 Agricultural Biotechnology
6. Biochemistry and Molecular Biology of Plants; Edited by Buchanan, Gruissem and Jones 2000,
, American Society of Plant Biologists, USA
7. Specific journals and published references
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at least
one from each unit. All questions will carry equal marks.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
3 Hours
D uration
Examination
of
1.
2.
3.
4.
5.
6.
7.
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
Credits
Class Work
50 Marks
--
Examination
50 Marks
Total
100 Marks
3 Hours
D uration
Examination
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
N ote:
of
The students w ill be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
BT 613 MS
BIOTECHNOLOGY LAB IX
Credits
Class Work
: 50 Marks
--
Examination
: 50 Marks
Total
: 100 Marks
Duration of Exam
: 3 hours
List of Experiments/Exercises
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
TEXT/REFERENCE BOOKS:
1.
2.
3.
4.
5.
J.H. Dodde and I.W. Robert : Experiments in Plant Tissue Culture, 1998.
H .S. Chaw la Practicals in Plant Biotechnology, ed ., Oxford & IBH Pu blications, N ew Delhi
2004
Ad rian Slater, N igel Scott and Mark Fow ler, Plant Biotechnology: The genetic m anipulation
of plants, 1st Edition, Oxford University Press,2003
Chrispeels , MJ and Sadava, DE, Plants, Genes and Crop Biotechnology 2nd edition, American
Society of Plant Biologists, Jones and Bartlett Publishers, USA,2003
Arie Altman, Marcel Dekker, Agricultural Biotechnology,2001
N ote: The students will be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory courses.
L
-
T
-
P
2
Credits
2
Class Work
Total
: 50 Marks
: 50 Marks
At the end of sem ester II each stu d ent w ou ld u nd ergo fou r w eeks Professional Training in an
Ind u stry/ Institu te/ Professional Organization/ Research Laboratory etc and su bm it in the
department a typed report along with a certificate from the organization.
The typed report should be in a prescribed format.
The report w ill be evalu ated in the III Sem ester by a Com m ittee consisting of three teachers from
d ifferent specialization to be constitu ted by the Chairperson of the d epartm ent. The basis of
evalu ation w ill prim arily be the know led ge and exp osu re of the stu d ent tow ard s d ifferent processes
and the functioning of the organization.
The stu d ent w ill interact w ith the com m ittee throu gh presentation to d em onstrate his/ her learning.
Teachers associated with evaluation work will be assigned two periods per week load.
Credits
Class Work
: 50 Marks
--
Examination
: 100 Marks
Total
: 150 Marks
D uration
Examination
of
: 3 Hours
UNIT-I:
Microbial
Pathogenesis:
Classification
of
m ed ically
im portant
m icroorganism s; N orm al m icrobial flora of hum an bod y; role of the resid ent
flora; Infection and its types, sou rces of infection. Transm ission of d iseases
anti-phagocytic factors; m echanism of bacterial ad hesion. Role of aggressins,
d epolym erising enzym es, organotropism s, viru lence and viru lence factors
Exotoxins and end otoxins Organs and cells of im m u ne system and Basic
immune response , Cell mediated and Humoral immunity.
UNIT-II:
UNIT-III:
Microbial Diseases Cau sed by Viru ses and Fu ngi: Med ically im portant
pathogenic Viruses: Orthom yxoviru ses, H epatitisviru ses, Oncogenicviru ses,
H IV viru ses: Med ically im portant pathogenic Fu ngi: Derm atop hytic &
Dim orphic fu ngi, Opp ortu nistic fu ngal Pathogens A brief accou nt of
Pathogenesis, virulence & treatment.
UNIT-IV:
Antim icrobial Chem otherapy: Anti- bacterial Chem otherap y u sing various
inhibitors. Anti-viral & Anti-fu ngal Chem otherapy, Dru g resistance , Gene
therapy, Vaccines, Antisense therapy, Enzym e and horm one therapy, RNA
based therapeutics.
Credits
Class Work
: 50 Marks
--
Examination
: 100 Marks
Total
: 150 Marks
Duration
Examination
UNIT-I:
UNIT-II:
UNIT-III:
UNIT-IV:
of
: 3 Hours
Basic in Anim al Biotechnology: Anim al cell lines, organ cell culture and
its applications. Anim al tissue cultu re - Plasm a clot m ethod , Raft
m ethod , Agar-gel m ethod , Grid m ethod , etc. - Cyclic exposu re to
Med ium and Gas phase, Ad vantages, Risk in anim al cell cu ltu re
laboratory-Biohazards
Anim al cell cu ltu re m ed ia: Preparation and Sterilization of cell cultu re
media and reagents. Introduction to the balance salt solutions and simple
grow th m ed ium . Brief d iscu ssion on the chem ical, p hysical and
metabolic functions of different constituents of culture medium. Facilities
for anim al cell cu ltu re-infrastru cture, equipm ent, cu ltu re vessels
equipment, culture vessels
Isolation, m aintenance and preservation of cell lines: Prim ary cell cultu re
techniques - m echanical d isaggregation, enzym atic d isaggregation,
separation of viable and non-viable cells. Mass cu ltu re of cells manipulation of cell line selection - types of cell lines -maintenance of cell
lines - immobilization of cells and its application - synchronization of cell
cu ltu res and cell d ivision - prod u ction of second ary m etabolitesbiotransformation - Ind uction of cell line m u tants and m u tations cryopreservation-germ plasm conservation and establishm ent of gene
banks.
Gene cloning and transgenic techniqu es: Techniqu es for m am m alian
cells, establishm ent of im m ortal cell lines, cloning in m am m alian cells,
expression of m am m alian genes in prokaryotic and eu karyotic system s,
extinction of gene fu nction by antisense RN A and DN A. Transfection
m ethod s, Em bryonic Stem Cell Transfer, Targetted Gene Transfer. Transgene integration, Detection of Transgenics throu gh Transgene
fu nction. Transgenic anim als:-Mice, Rabbits, Cattle, Goat, Sheep , Pigs
and Fish.
Credits
Class Work
50 Marks
--
Examination
100 Marks
150 Marks
3 Hours
Total
D uration
Examination
of
UNIT-I:
UNIT-II:
UNIT-III:
Environm ental Pollu tion: Concept of Environm ental Pollu tion; Origin of
pollu tion; Classification and natu re of Environm ental Pollu tants; Major air
pollu tants and their sou rces; Particu late m atter; Form ation of fog and
photochem ical sm og and acid rain; Typ es, sou rces and consequ ences of w ater
pollu tion, ecological and biochem ical asp ects of w ater pollu tion ; w ater
pollu tion control.; Types and possible hazard s of rad ioactive su bstances;
Effects of rad ioactive w aste pollu tion on environm ent and im pact of rad iation
on life; Concept of soil pollu tion; Control of soil pollu tion; Concept of solid
w aste; Major sou rces of solid w astes; Technical ap proach for solid w aste
management.
UNIT- IV:
Environm ent Qu ality control: Qu ality of environm ent for life on earth and
m an; Deterioration of environm ental quality w ith reference to anthropogenic
im pact; Method s of assessm ent of environm ental quality; Concep t of Rem ote
sensing; App lication of rem ote sensing in environm ental stu d ies; Concept of
Geographical Inform ation System ; Types of Geographical Data; Im portance of
GIS in environmental studies
BT- 608 MS
Credits
Class Work
50 Marks
--
Examination
100 Marks
Total
150 Marks
3 Hours
D uration
Examination
of
UNIT-I:
UNIT-II:
UNIT-III:
UNIT-IV:
N OTE: In the semester examination, the examiner w ill set eight questions in all, selecting tw o
from each unit. The candidates w ill be required to attempt five questions in all selecting at least
one from each unit. All questions will carry equal marks.
BT 610 MS
BIOTECHNOLOGY LAB X
Credits
Class Work
: 50 Marks
--
Examination
: 50 Marks
Total
: 100 Marks
Duration of Exam
: 3 hours
List of Experiments/Exercises
1.
2.
Sterilization of plastic ware and glass ware used in animal cell culture.
To d em onstrate the principle and fu nctioning of com m only used instrum ents in anim al
biotechnology lab.
Preparation of various media used for animal cell culturing.
Trypsinization of monolayer and subculturing.
To perform SRID, DID for disease diagnosis.
Immunoelectrophoresis for detection of pathogens.
To perform biochemical tests for identification of pathogens.
To detect blood groups of the given samples.
To perform TLC, DLC of the given blood samples.
To study the microbial flora of skin.
To determine the microbial content of dental cavities.
To perform widal for diagnosis of typhoid.
To perform VDRL for diagnosis of syphilis.
To demonstrate the effect of different antibiotics on bacterial pathogens.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
N ote:
The students w ill be required to perform 08 experiments/ exercises from the above list and
the other tw o experiments may be designed by the department based on the related
theory course.
BT 612 MS
BIOTECHNOLOGY LAB XI
Credits
Class Work
: 50 Marks
--
Examination
: 50 Marks
Total
: 100 Marks
Duration of Exam
: 3 hours
List of Experiments/Exercises
1.
2.
3.
NOTE: The students will be required to perform the experiments/exercises from the above list
and any two experiments designed by the department based on the theory course.
BT 614 MS
Credits
Class Work
: 50 Marks
--
Total
: 50 Marks