Professional Documents
Culture Documents
ABSTRACT
The marine environment as a huge basket contains many harmful substances. One of the
contamination agents causes the marine environment serious consequences such as heavy metals.
This study, we reported the results of the determination of total concentration arsenic in some sea fish
samples in Da Nang City by using atomic absorption spectrometry (AAS) showed that arsenic is
presented in all the analyzed samples. Concentrations arsenic in the range of 0.015mg/kg to
0.214mg/kg.
Key words: Arsenic, concentration, sea fishes.
1. Introduction
The polluted problem of the marine environment in Vietnam is becoming increasingly serious day by
day. Several heavy metals (As, Hg, Fe, Pb,...) are in everywhere in marine population, especially is
the presence of arsenic and its compounds in some sea fishes. Sea fishes are aquatic animals with
high economic value, as food sources are abundant and rich in nutrients. As a result, they are widely
used in Vietnam and other countries in the world [2]. However, the understanding of Vietnamese on
harmful effects of arsenic and its compounds is extreme limit. This leads the prevention of arsenic
polution in the community has not been high [3]. As can be seen that, arsenic and its compounds are
extremely toxic substances. It can cause the death when we are acute infection and with chronic
infection from arsenic and its compounds can cause a variety of serious diseases, including skin and
lung cancer. For these reasons, the anlysis and evaluation arsenic and its compounds are an
imperative problem to contribute positively to Vietnams food safety programs [4,5].
The purpose of this study was to analyse and evaluate the total concentration of arsenic and its
compounds in the sea fishes on the Danang beaches by using AAS [1]. Our study provided an exact
data about arsenic polution in sea fishes in Vietnam.
___________________________________________
* Corresponding author
Email adress: lethimui@gmail.com
Office adress: 459 Ton Duc Thang st., Lien Chieu dist., Danang city, Vietnam
2. Experimental procedure
Some sea fishes were used in the study
Sea fishes purchased from the markets on Danang City and washed with distilled water. Using a
stainless knife separated the fish meat for analysis process. The fish meat washed with distilled water
before being dried. Then the samples grinded to smooth powder. The samples continued to
disintegrate at high temperature by the mixture of clohydric and sulfuric acid (HCl 62% and H2SO4
96%, China). At the same time, all of arsenic compounds were converted to As+3 using NaBH4 (95%,
China) as a reducing agent. The concentration of arsenic and its compounds in sea fishes were
determined through As+3 concentration by using AAS (Perkin Elmer, USA) method.
3. Results and discussions
To construct procedures for analysis of arsenic content in sea fish samples, we conducted inorganic
process for wet sample by using acid mixture. Accordingly, the content of As was determined by
AAS method. The principle of the method is conversion of all arsenic in the samples into the ions in
solution and then using NaBH4 reductant to convert all arsenic compounds to the hydride compound
(AsH3).
Determination content
Arsenic (mg/l)
Performance
(%)
0.05
0.0426
85.2
0.05
0.0397
79.4
0.05
0.0453
90.6
0.05
0.0463
92.6
0.05
0.0478
95.6
Average performance
88.68
Variance
Average
value
S2
Standard
deviation
Coefficient
of variation
Standard
error
Confiden
t border
Relative
Cv (%)
Sx
errors(%)
As3+
0,04
(ppm)
0,0384
4,48.10-9
6,69.10-5
1,74
2,99.10-5
8,31.10-5
2,17
As3+
0,06
(ppm)
0,0529
2,45.10-9
4,95.10-5
0,94
2,21.10-5
6,14.10-5
1,16
Order
number
1
2
3
4
5
Salangichthys
microdon
Decapterus
0.078
7
maruadsi
Harpadon
0.076
8
nehereus
Teleostei Percida
Polynemus
0.101
9
borneensis
Plesiotrygon iwamae
Selaroides
0.214
10
leptolepis
National Technical Regulation on Ministry of Public Health
0.015
Arsenic content
(mg/kg)
0.032
0.072
0.097
0.193
0.058
1
4. Conclusion
1. Having survey the solvent was used to treat samples sea fishes is the mixture of HCl: HNO3 (3: 1
by volume ratio): 15ml HCl concentrate, 5ml HNO3 concentrate .
2. Determining recovery yield of method, the results show recovery achieved 88.68%, the method
has high precision, good repeatability.
3. On the basis of the factors examined were proposed procedures analytic arsenic in sea fishes with
Atomic Absorption Spectrophotometric.
4. Applying procedures analytic to determine content of total arsenic in some sea fishes in Da Nang
city. The analysis results shows that most of the fish samples contain content of arsenic.
Plesiotrygon iwamae and polynemus borneensis have the highest content of arsenic but all
samples degree of safety is fall within the scope of the regulation
References
1. L. S. Clescerl, A. E. Greenberg, A. D. Eaton, Standard methods for the examination of water and
wastewater, 20th Edition. Washington DC, USA, (1999).
2. M. O. Andreae, Distribution and speciation of asenrnic in natural water and some marine algae,
(1978), 391-402.
3. W. R. Atkins, E. Wilson, The phosphorus and arsenic compounds of sea water, J Mar. Biol.
Assoc. UK, (14) (1926), 609-614.
4. H. Mukai and Y. Ambe, Determination of methylarsenic compounds in airborne particulate
matter by gas chromatography with atomic absorption spectrometry, Anal. Chim. Acta, (193),
(1987), 219-229.
5. J. S. Edmonds, Y. Shibata, K. A. Francesconi, R. J.Rippingale, M. Morita, Arsenic
transformations in short marine food chains studied by HPLC- ICP- MS, (11),(1997) 281 287.