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Original Article
A R T I C L E I N F O
A B S T R A C T
Article history:
Received 19 November 2009
Received in revised form 12 April 2010
Accepted 12 June 2010
This investigation reports the variability of antioxidant components and antioxidant activities of six
watermelon cultivars (cvs) (four commercial cvs Aramis, Crimson Sweet, Dumara, Giza, and two new
selections P503 and P403 produced by the National Agricultural Research Institute of Tunisia) as
inuenced by sampling area. All cvs were simultaneously grown in an open-eld and subjected to
identical horticultural practices in order to minimize the effects of environmental conditions and
maximize those related to genotype. Signicant differences were found between watermelon cvs for
lycopene, phenolics, avonoids, ascorbic acid (AsA), dehydroascorbic acid (DHA) and total vitamin C
(AsA + DHA) contents, as well as in the antioxidant activity of their hydrophilic and lipophilic fractions.
P503 cv showed the highest lycopene and avonoid contents. Crimson Sweet and Giza cvs showed the
highest HAA and LAA when TEAC was used as assay method, while the highest HAA and LAA were
detected in Giza and Dumara cvs and in P503 cv, respectively, when FRAP assay was used. This study
demonstrates that the amount of each specic antioxidant, as well as the HAA and LAA, were both
inuenced by genotype and sampling area, emphasizing the need to evaluate watermelon biodiversity in
order to improve its nutritional value.
2010 Elsevier Inc. All rights reserved.
Keywords:
Citrullus lanatus (Thunb.) Mansfeld
Antioxidant activity
Flavonoids
Lycopene
Phenolics
Sampling area
Vitamin C
Watermelon
Biodiveristy and nutrition
Horticulture
Cultivar difference
Bioavailability
Food analysis
Food composition
1. Introduction
Today, there is an increasing grower and consumer demand for
high-quality, healthful foods and food products. Watermelon
(Citrullus lanatus (Thunb.) Mansfeld) is a popular fruit endowed
with high natural antioxidant capacity, an attribute which is
becoming an important qualitative factor for foods.
Watermelon is one of the main vegetable crops grown and
consumed all over the Mediterranean basin. It is much appreciated as
an excellent refreshing summer fruit. Besides vitamins (A, B, C and E),
mineral salts (K, Mg, Ca and Fe), and specic amino acids (citrulline
308
contains moderate but signicant quantities of phenolics (PerkinsVeazie et al., 2002; Brat et al., 2006). These key secondary metabolites
exhibit highly efcient peroxyl-radical scavenging activity and hence
potential pharmacological effects (Larson, 1988; Halliwell, 1994;
Manach et al., 1998). Among phenolics, avonoids reduce low
density lipoprotein (LDL) oxidation and quench reactive oxygen
radicals, decreasing thereby the risk of cardiovascular diseases and
cancers (Pietta, 2000; Lila, 2004). Although the biological effect of
avonoids is, in general, attributed to their antioxidant activity,
recent investigations indicate that they might affect signalling
pathways in animal cells (Williams et al., 2004).
Watermelon fruits have been identied as a good source of
vitamin C, mainly in the reduced form of AsA (Vanderslice et al.,
1990). In addition to its antioxidant activity against free radicals,
AsA has numerous biological functions, which include the
synthesis of collagen, steroid and peptide hormones and neurotransmitters (Cameron et al., 1979).
It is known that the amount of each antioxidant in fruits and
vegetables is strongly inuenced by genotype differences and
external factors such as agro-technical processes, environmental
conditions, ripening degree at harvest and post harvest manipulation (Waterman and Mole, 1994; Abushita et al., 2000; Dumas et al.,
2003). Few studies have been focused on the physicchemical
properties and antioxidant components of watermelon cvs.
Recently, Perkins-Veazie et al. (2006) and Perkins-Veazie and Davis
(2007) emphasized the importance of genotype and sampling areas
when assessing the lycopene and soluble solid content in watermelons. They also suggested the need to adopt standardized and
documented sampling methods when assessing quality attributes in
watermelon fruits. In fact, in large fruits, as watermelon, where only
a portion of the esh is feasibly tested for quality, an accurate and
reproducible sampling method must be developed.
The amount of a determined antioxidant compound in a food
does not necessarily correlate with its antioxidant activity. Due to
the complexity of the composition of foods, their antioxidant
power depends on the synergistic effects and redox interactions
among the different nutrient and non nutrient molecules which
together contribute to the supposed health benets. Therefore, the
use of methods to measure the total antioxidant activity (TAA) of
foods and biological samples is very appealing to researchers
(Huang et al., 2005).
The aim of this study was to investigate the inuence of
genotypic differences and fruit sampling area on the amounts of
lycopene, total phenolics, avonoids, AsA, DHA and total vitamin C
in the esh of different cvs of watermelon grown in Tunisia and to
characterize their in vitro hydrophilic and lipophilic antioxidant
activities.
()TD$FIG][of
309
310
Lycopene
(mg/kg fw)
Total phenolics
(mg GAE/kg fw)
Flavonoids
(mg RE/kg fw)
52.7 0.2a
44.6 3.1a
60.3 6.9a
56.3 8.5a
53.5C
143.3 3.8ab
145.4 6.1a
92.8 1.8c
127.2 6.2b
127.2B
163.5 7.7b
194.6 2.4a
197.4 6.5a
148.9 6.4b
176.1A
90.1 5.6b
103.0 5.8ab
105.3 4.3a
89.1 1.7b
96.9A
180.1 2.0a
155.9 2.8b
127.2 6.0c
126.1 2.3c
147.3A
179.2 3.3ab
169.2 2.4bc
188.6 6.7a
162.6 8.3c
174.9A
41.2 0.7b
43.8 2.0ab
47.4 1.7a
38.3 0.8b
42.7D
128.6 2.3a
98.7 2.8a
94.2 5.2b
124.1 6.2b
111.4C
121.6 2.4a
130.3 3.7a
120.7 2.1ab
101.1 5.8b
118.4B
43.7 2.3a
46.5 3.6a
48.5 1.9a
42.7 0.4a
45.4CD
119.9 1.8b
135.5 3.7a
113.3 4.4b
92.1 4.3c
115.2C
120.6 3.2ab
94.2 6.5c
104.7 2.5bc
132.7 4.0a
113.0B
100.3 1.9b
112.7 0.7a
112.0 2.2a
84.5 3.7c
102.4A
69.3 4.0b
96.8 2.6a
89.7 4.7a
100.3 3.5a
89.0D
193.8 1.8a
177.3 6.1a
180.7 6.9a
149.1 2.7b
175.2A
92.4 4.7a
86.7 2.5a
95.9 4.5a
94.4 3.6a
92.3D
95.0 2.9b
105.0 2.1b
149.6 2.5a
95.7 1.9b
111.3B
67.0 3.9ab
77.7 9.1ab
81.2 2.2a
58.2 1.4b
71.0B
Lower case letters indicate mean separation within column and sampling area by
LSD test, P < 0.05. Capital letters indicate mean separation among means within
column by LSD test, P < 0.05.
Antioxidant compounds
Lycopene (mg/kg fw)
Total phenolics
(mg GAE/kg fw)
Flavonoids
(mg RE/kg fw)
AsA (mg/kg fw)
DHA (mg/kg fw)
Total vitamin C
(mg/kg fw)
Blossomend
Stemend
Heart
Peripheral
65.8B
122.3A
71.37A
119.8A
75.8A
102.2C
61.5B
110.7B
145.6B
145.1B
157.0A
131.7C
76.2C
89.7B
165.9B
95.6A
90.4B
186.0A
63.8D
105.0A
168.7B
84.4B
103.3A
187.7A
281.5A
257.8A
193.2B
236.3B
206.0B
219.6C
30.2A
33.1B
22.6C
29.4C
23.9B
37.4A
Antioxidant activity
TEAC assay (mmol Trolox/100 g fw)
HAA
261.9A
LAA
236.5B
FRAP assay (mM FRAP/g fw)
HAA
22.9C
LAA
29.3C
In each row, values with the same letters are not signicantly different (LSD test,
P < 0.05).
AsA
(mg/kg fw)
311
fw) and
blossom-end
DHA
(mg/kg fw)
Total vitamin C
(mg/kg fw)
75.1 9.3ab
88.1 5.4a
67.7 4.1b
65.0 1.9b
73.9C
105.0 4.1a
69.3 12.9b
118.2 3.8a
65.1 6.8b
89.4C
180.1 16.6ab
157.4 8.6b
185.9 4.7a
130.1 0.4c
163.4C
160.9 5.8a
144. 7 9.4ab
84.0 1.2c
121.8 4.9b
127.8A
61.1 1.9c
107.9 7.0b
107.0 4.3b
140.0 1.9a
104.0B
222.0 16.3b
252.6 8.64a
191.0 6.84c
261.8 13.69a
231.8A
135.1 3.8b
146.2 4.4a
120.7 3.9c
127.7 1.8bc
132.4A
131.2 7.2a
85.0 4.3b
95.3 3.5b
117.8 7.1a
107.3B
266.3 10.61a
231.2 13.12bc
216.0 0.57c
245.6 8.81b
239.8A
22.5 0.4b
24.2 0.9b
10.8 1.2c
33.1 1.7a
22.7E
108.1 7.7c
150.0 8.4b
203.8 10.6a
214.6 6.8a
169.1A
130.6 13.06d
174.3 3.29c
214.6 10.34b
247.8 8.30a
191.8B
38.2 5.3c
113.1 6.8a
75.3 3.4b
110.2 1.2a
84.2B
72.8 10.4a
63.7 10.6a
22.7 5.2b
26.2 3.6b
46.4E
111.1 3.9c
176.8 5.6a
98.0 6.0c
136.4 3.4b
130.6D
60.0 9.2a
66.5 1.9a
82.6 2.0a
56.3 2.0a
66.4D
85.5 3.0a
123.7 9.2a
106.7 4.9a
104.8 1.5a
105.2E
25.5 1.0c
75.2 2.3a
24.1 2.9c
48.6 1.5b
38.8D
Lower case letters indicate mean separation within column and sampling area by
LSD test, P < 0.05. Capital letters indicate mean separation among means within
column by LSD test, P < 0.05.
312
Table 4
Hydrophilic and lipophilic antioxidant activities of the watermelon cvs studied within different sampling areas.
Cultivars
Crimson Sweet
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
Giza
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
Dumara
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
P403
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
P503
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
Aramis
Blossom-end area
Stem-end area
Heart area
Peripheral area
Mean
Hydrophilic
Lipophilic
Hydrophilic
Lipophilic
311.4 7.8a
342.4 5.2a
215.1 5.2b
294.8 5.4a
290.9A
246.7 4.4b
271.9 4.4a
284.0 4.5a
233.2 4.7b
258.9A
13.4 0.3c
32.1 0.1a
24.1 0.4b
24.0 0.1b
23.4D
25.2 0.4a
21.9 0.6b
14.4 0.5c
26.6 0.3a
22.0E
325.0 4.0a
350.2 5.2a
201.1 4.3b
209.0 7.7b
271.3A
274.1 4.6a
259.7 5.7a
260.2 8.9a
230.1 9.1b
256.1AB
28.7 0.3c
32.6 0.3a
27.7 0.2d
30.6 0.1b
29.9A
23.9 2.6b
25.1 0.8b
33.8 0.8a
33.8 0.7a
29.2D
254.2 4.8a
229.0 4.9a
156.4 7.8b
170.5 5.1b
202.5D
247.8 3.2a
244.5 3.8a
256.1 5.4a
223.1 6.5b
242.9C
34.8 0.25a
32.1 0.26b
23. 6 0.25d
27.8 0.27c
29.6A
39.2 0.4b
41.7 0.6a
28.4 0.3c
27.1 0.2c
34.1BC
297.3 11.6a
267.2 5.7ab
185.2 8.7c
221.8 6.6bc
242.9C
269.2 4.2a
298.8 4.8b
195.2 7.6c
239.4 10.0d
250.7B
30.0 0.2b
32.2 0.1a
24.7 0.2c
23.0 0.2d
27.5B
31.4 2.6b
36.8 0.8a
33.9 0.6ab
38.4 0.5a
25.1B
182.2 3.2a
211.4 5.7a
161.5 6.1a
174.3 4.3a
182.4E
196.6 3.8a
208.3 5.5a
217.7 3.2a
201. 5 7.0a
206.0D
13.7 0.3b
13.2 0.2b
10.3 0.2c
15.9 0.2a
13.3E
33.5 0.5b
29.9 0.2c
34.7 1.1b
63.1 0.6a
40.3A
201.3 4.8bc
292.2 8.7a
239.9 5.6b
169.1 4.3c
225.7C
184.3 7.6c
263.5 5.3a
204.4 4.9b
190.5 3.3bc
210.7D
16.7 0.3d
39.2 0.1a
25.5 0.3b
21.9 0.3c
25.8C
22.3 0.8c
43.2 2.3a
31.5 1.1b
35.5 0.4b
33.1C
Lower case letters indicate mean separation within column and sampling area by LSD test, P < 0.05. Capital letters indicate mean separation among means within column by
LSD test, P < 0.05.
Table 5
Correlation coefcients and related signicances between antioxidant compounds and antioxidant activities; n (sample size) = 72.
Antioxidants
HAA TEAC
HAA FRAP
Corr coeff
Ascorbic acid
Dehydroascorbic acid
Total vitamin C
Total phenolics
Flavonoids
Antioxidant
Lycopene
ns, no signicant correlation.
0.133
0.012
0.116
0.637
0.352
ns
ns
ns
<0.01
<0.01
LAA TEAC
Corr coeff
0.212
0.320
0.434
0.400
0.264
P
ns
<0.01
<0.01
<0.01
ns
LAA FRAP
Corr coeff
Corr coeff
0.230
ns
0.063
ns
313
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