DEPARTMENT OF BIOCHEMICAL ENGINEERING AND BIOTECHNOLOGY

Maior .~xalllination
BEL311: Physicaland ChemicalI'n)pcrtiesof Biom?lecules
Max. Marks: 45 Time: 2 hom',

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I. Show that the wavelength of the emitted photons cannot be longer than the wavelength of t'te cxeitaliull
photons Il)1a Iluorophore.
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2. Given a microbial cell. provide a protocol (in form of a flow diagram/table) to purify intraccllul:,:
proteins. What would be tbe major difference in the methodology for purifying membrane proteili
Clearly mark the sleps in the t1ow diagramltable for answering the latter.
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3. What is thc Iluid-mosaic modd It)[biologicalmembrancs'?What are memhrtme rails? Whal hiocltcll1i..
and hi(Iphysical experimental evidence den}onstratcsthe presenceor membrane raIts.
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4. Biomolccular asymmetry is an inherent feature of biological membranes. \-vhen they ::n~a part of a livil1!'
system. List the asymmetries. Why are these asymmetries abs,.;;nt in syntheti!:~ systems (;'.g. liposol1ll:s\ '
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5. Nam<:the Wl.:akand strong interactions of biolllo\ecuies. Why is it import:!:;: that v,::'" illteractiolh. P' 'f
Slrolig (lm~s.mediate biomolecular interactions including recognition'?
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(), What ;IIT the limitations of SDS-PAGE? Why does one require a cross::ilkl'r 10 i.kllt;:\' I)fiJI"1I1
L'OIl1j1k"l'S hy SDS-PAGE'?
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7, J\ microlll' having biological membranes of thickncss of I nlll has been di'-:l"c)VC]"!i, \ (ltal !n!!; II!
analyscs l'wlII that microbe reveal the same components for protein ttmlwticn as di~';" '\ ':1 vd in g\.lIl'i ,d
till date. It i:; hypothesized that protein folding in the microbelt)IlO\vs the sam(' gelll:rai principles tl1;)1
are \Vcll ;lCCl'ptcd so far. Analysis or the whole c(:lllysatL' rcsul\/:d in a large aliHillnt ot";\ 1(1\\ Ilwkctil:'

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(us\.' the hydr:')rrl~)bicltyscale pro\tided below):
t ISeq~lencing could ren:;:! only a porlion
Answerthe following qucstiol1srcg:mlinL~thc prolt';I:
of the protein a'.

,S if (a) How many amino acid residues ,:'ould be expected.in the ~ltllprotein?
~ (:) t (b) I\()\v n~any transmembrane dOI~lallls. docs the protell1 ~i~ve?
I . r.cr~ (c) :X-s~umlJ1gthat the values prov.lded III the hydror:hob!clty sca\c bd{)\\' .represent ~l1alll "
( () G~' In free energy, what can be saId ahout the expertmeptallsyslem used lo!' gcneratlllt: 111
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~biCitY scale given by Eisenberg et aI., j, Mol. BioI. (1984): t' I
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