Chemwell 2902 / 2910

Instrument Application

Test: Uric Acid

Catalog # : U7581

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Uric Acid
Chemistry
Standard
0
37 C
2.5 7.7
<= 20, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Uric Acid Reagent], Volume [200.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Add Sample, Volume [5.0 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=High
Incubate 00:10:00 [0h 10min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Document number: APP-U7581-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Albumin
Catalog # : A7502

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Albumin
Chemistry
Standard
0
37 C
3.5 5.3
0.5 8.0, Use Reflex Test, Dilution Ratio = 2
g/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Albumin Reagent], Volume [200.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1 min 0 sec]
Read with Primary Filter [630], Differential Filter [700]
Add Sample, Volume [2.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:01:00 [0h 1 min 0 sec]
Read with Primary Filter [630], Differential Filter [700]
Document number: APP-A7502-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Alkaline Phosphatase

Catalog # : A7516

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Alk Phos
Chemistry
Factor
Factor = 2187 (Adjusted 3988.1)
0
37 C
35 123
<= 800, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [5.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [Alk Phos working Reagent], Volume [200.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1 min 0 sec]
Kinetic Read, Filters [450/630], Read Interval [60sec], 3 Times
Initial Absorbance < 1.000
Delta Absorbance per Minute < 0.3657 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-A7516-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: ALT (SGPT)

Catalog # : A7526

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

ALT (SGPT)
Chemistry
Factor
Factor = -1768 (Adjusted -3004.2)
0
37 C
4 36
<= 500, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [20.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [ALT working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:30 [0h 1min 30sec]
Kinetic Read, Filters [340/405], Read Interval [60sec], 4 Times
0.8000 < Initial Absorbance
Delta Absorbance per Minute < 0.1700 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-A7526-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Amylase
Catalog # : A7564

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Amylase
Chemistry
Factor
Factor = 3178 (Adjusted 5795.3)
0
37 C
25 125
<= 2000, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [5.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [Amylase Reagent], Volume [200.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Kinetic Read, Filters [405/630], Read Interval [60sec], 3 Times
Delta Absorbance per Minute < 0.6290 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-A7564-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: AST (SGOT)

Catalog # : A7561

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

AST (SGOT)
Chemistry
Factor
Factor = -1768 (Adjusted -3004.2)
0
37 C
5 34
<= 500, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [20.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [AST working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:30 [0h 1min 30sec]
Kinetic Read, Filters [340/405], Read Interval [60sec], 4 Times
0.8000 < Initial Absorbance
Delta Absorbance per Minute < 0.1700 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-A7561-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: auto HDL

Catalog # : H7545

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

HDL
Chemistry
Standard
0
37 C
30 85
2 150, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
autoHDL/LDL Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [3.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [HDL R1 Reagent], Volume [180.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Add Reagent [HDL R2 Reagent], Volume [60.0 ul]
Aspiration Speed=0, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Document number: APP-H7545-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Direct Bilirubin

Catalog # : B7538

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Direct Bilirubin
Chemistry
Standard
0
37 C
0.0 0.5
<= 20.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [25.0 ul]
Aspiration Speed=4, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [Direct Bilirubin R1], Volume [180.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Add Reagent [Direct Bilirubin R2], Volume [20.0 ul]
Aspiration Speed=1, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Document number: APP-B7538-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Total Bilirubin

Catalog # : B7576

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Total Bilirubin
Chemistry
Standard
0
37 C
0.2 1.2
<= 20.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Total Bilirubin working Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [630]
Add Sample, Volume [10.0 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [545], Differential Filter [630]

Document number: APP-B7576-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: BUN
Catalog # : B7552

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

BUN
Chemistry
Standard
0
37 C
7 18
<= 150, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [2.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [BUN working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:15 [0h 1min 15sec]
Kinetic Read, Filters [340/630], Fixed Time [2min 30sec]

Document number: APP-B7552-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Calcium AZ II
Catalog # : C7529

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Ca AZ II
Chemistry
Standard
0
37 C
8.5 10.4
<= 15.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Calcium AZ II Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Add Sample, Volume [2.0 ul]
Aspiration Speed=1, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:03:00 [0h 3min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Document number: APP-C7529-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Calcium CPC

Catalog # : C7503

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Calcium CPC
Chemistry
Standard
0
37 C
8.5 10.5
<= 20.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Calcium Color Reagent], Volume [100.0 ul]
Aspiration Speed=2, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Add Reagent [Calcium Buffer Reagent], Volume [100.0 ul]
Aspiration Speed=2, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Add Sample, Volume [4.0 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Document number: APP-C7503-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: CK
Catalog # : C7522

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

CK
Chemistry
Factor
Factor = 6592 (Adjusted 12021.0)
0
37 C
<= 160
<= 1500, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [5.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [CK working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:02:00 [0h 2 min 0 sec]
Kinetic Read, Filters [340/405], Read Interval [60sec], 2 Times

Document number: APP-C7522-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Chloride
Catalog # : C7501

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Chloride
Chemistry
Standard
0
37 C
98 106
80 120, Use Reflex Test, Dilution Ratio = 2
mEq/L
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Chloride Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Add Sample, Volume [2.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=1, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [505], Differential Filter [630]

Document number: APP-C7501-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: CO2
Catalog # : C7502

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

CO2
Chemistry
Standard
0
37 C
23 34
2 - 40, Use Reflex Test, Dilution Ratio = 2
mEq/L
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [2.0 ul]
Aspiration Speed=1, Air Gap=2ul, Dispense Speed=1, Dispense Height=Low
Add Reagent [CO2 Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:30 [0h 1min 30sec]
Kinetic Read, Filters [405/505], Fixed Time [5min 0sec]
Document number: APP-C7502-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Cholesterol
Catalog # : C7510

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Cholesterol
Chemistry
Standard
0
37 C
< 200
<=700, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Cholesterol Reagent], Volume [240.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Add Sample, Volume [2.4 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:10:00 [0h 10min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Document number: APP-C7510-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Creatinine
Catalog # : C7539

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Creatinine
Chemistry
Standard
0
37 C
0.4 1.4
<= 25.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [10.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low
Add Reagent [Creatinine working Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=1, Dispense Height=High
Incubate 00:00:50 [0h 0min 50sec]
Kinetic Read, Filters [505/630], Fixed Time [2min 30sec]

Document number: APP-C7539-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Phosphorus
Catalog # : P7516

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Phosphorus
Chemistry
Standard
0
37 C
2.5 4.8
<= 12.0, Use Reflex Test, Dilution Ratio = 2
mg/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Phosphorus Reagent], Volume [200.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [340], Differential Filter [630]
Add Sample, Volume [4.0 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=Low
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [340], Differential Filter [405]
Document number: APP-P7516-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Gamma GT
Catalog # : G7571

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

GGT
Chemistry
Factor
Factor = 2211 (Adjusted 3935.9)
0
37 C
9 54
<= 800, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [10.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [GGT working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:30 [0h 1min 30sec]
Kinetic Read, Filters [405/630], Read Interval [60sec], 3 Times
Initial Absorbance < 0.8500
Delta Absorbance per Minute < 0.4520 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-G7571-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Glucose (Hex)

Catalog # : G7517

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Glucose (Hex)
Chemistry
Standard
0
37 C
70 110
<= 500, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Glucose (Hex) Reagent], Volume [240.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [340], Differential Filter [405]
Add Sample, Volume [2.4 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [340], Differential Filter [405]
Document number: APP-G7517-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Glucose (Ox)

Catalog # : G7521

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Glucose (Ox)
Chemistry
Standard
0
37 C
70 105
<= 500, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Glucose (Ox) Reagent], Volume [240.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Add Sample, Volume [2.4 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:10:00 [0h 10min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Document number: APP-G7521-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: HbA1c
Catalog # : H7541

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

HbA1c
Chemistry
Cubic Spline
1
37 C
< 6%
2 16
%
1

********************************************************************************************************************
Blank used:
Valid interval: 14 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
autoHDL/LDL Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [5.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [HBa1c R1 Reagent], Volume [150.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Add Reagent [HbA1c R2 Reagent], Volume [50.0 ul]
Aspiration Speed=0, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [630], Differential Filter [None]
Document number: APP-H7541-CW2910 Rev. 7-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Iron, Total

Catalog # : I7505

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Iron, Total
Chemistry
Standard
0
37 C
60 150
<= 500, Use Reflex Test, Dilution Ratio = 2
ug/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [30.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=1, Dispense Height=Low

Add Reagent [Iron Buffer Reagent], Volume [150.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Add Reagent [Iron Color Reagent], Volume [3.0 ul]
Aspiration Speed=2, Air Gap=2ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:10:00 [0h 10min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Document number: APP-I7505-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: LDH
Catalog # : L7572

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

LDH
Chemistry
Factor
Factor = 3376 (Adjusted 6010.0)
0
37 C
80 285
<= 1000, Use Reflex Test, Dilution Ratio = 2
U/L
0

********************************************************************************************************************
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [5.0 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [LDH working Reagent], Volume [200.0 ul]

Aspiration Speed=1, Air Gap=50ul, Prewarm Time=15sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:30 [0h 1min 30sec]
Kinetic Read, Filters [340/405], Read Interval [60sec], 4 Times
0.8000 < Initial Absorbance
Delta Absorbance per Minute < 0.1210 Use Reflex Test. Dilution Ration = 2
Maximum SD of Delta Absorbance per Minute = 0.0300
Adjust absorbance ranges to path length.

Document number: APP-L7572-CW2910 Rev. 6-12

Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Lipase
Catalog # : L7503

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Lipase
Chemistry
Standard
0
37 C
0 62
<= 600, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Lipase Standard, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Sample, Volume [2.5 ul]
Aspiration Speed=4, Air Gap=1ul, Dispense Speed=2, Dispense Height=Low

Add Reagent [Lipase Substrate (R1)], Volume [150.0 ul]

Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:04:00 [0h 4min 0sec]
Add Reagent [Lipase Activator (R2)], Volume [50.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:02:00 [0h 2min 0sec]
Kinetic Read, Filters [545/700], Fixed Time [1min 0sec]
Document number: APP-L7503-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Magnesium
Catalog # : HM929

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Magnesium
Chemistry
Standard
0
37 C
1.5 2.6
<= 4.86, Use Reflex Test, Dilution Ratio = 2
mg/dl
2

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours
Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Magnesium R1 Reagent], Volume [150.0 ul]
Aspiration Speed=2, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Add Reagent [Magnesium R2 Reagent], Volume [150.0 ul]
Aspiration Speed=2, Air Gap=25ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Add Sample, Volume [2.0 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Document number: APP-HM929-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Microprotein
Catalog # : P7582

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Microprotein
Chemistry
Standard
0
37 C
28 141
2 250, Use Reflex Test, Dilution Ratio = 2
mg/24 hrs
0
for CSF and Urine

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Microprotein Standard, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Microprotein Reagent], Volume [200.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Add Sample, Volume [4.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [600], Differential Filter [700]
Document number: APP-P7582-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Total Protein

Catalog # : T7528

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Total Protein
Chemistry
Standard
0
37 C
6.2 8.5
1.0 15.0, Use Reflex Test, Dilution Ratio = 2
g/dl
1

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Total Protein Reagent], Volume [200.0 ul]
Aspiration Speed=2, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=2, Dispense Height=High
Incubate 00:01:00 [0h 1min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Add Sample, Volume [4.0 ul]
Aspiration Speed=2, Air Gap=2ul, Dispense Speed=2, Dispense Height=Low
Incubate 00:05:00 [0h 5min 0sec]
Read with Primary Filter [545], Differential Filter [700]
Document number: APP-T7528-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com

Chemwell 2902 / 2910

Instrument Application

Test: Triglycerides
Catalog # : T7532

The following reagent parameters are provided by Pointe Scientific, Inc as a reference when Pointe Scientific reagents are used on this
instrument. This application has not been validated by Pointe and should only be used in conjunction with your laboratory quality control
validation program.

Assay Name:
Assay Type:
Assay Mode:
Version:
Temperature:
Normal:
Linear:
Units:
# of decimals:
Assay Description:

Triglycerides
Chemistry
Standard
0
37 C
44 148
<= 1000, Use Reflex Test, Dilution Ratio = 2
mg/dl
0

********************************************************************************************************************
Blank used:
Valid interval: 7 days 0 hours

Controls:
Level 1, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Level 2, Warn & Continue if out of range, Valid interval: 1 day 0 hours
Standards:
Chemistry Calibrator, Curve valid interval: 7 days 0 hours

********************************************************************************************************************
ASSAY STEPS
Add Reagent [Triglycerides Reagent], Volume [200.0 ul]
Aspiration Speed=1, Air Gap=50ul, Prewarm Time=0sec, Dispense Speed=1, Dispense Height=High
Incubate 00:02:00 [0h 2min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Add Sample, Volume [2.5 ul]
Aspiration Speed=2, Air Gap=1ul, Dispense Speed=1, Dispense Height=High
Incubate 00:10:00 [0h 10min 0sec]
Read with Primary Filter [505], Differential Filter [630]
Document number: APP-T7532-CW2910 Rev. 6-12
Phone: 734-487-8300 Toll Free: 800-757-5313 Fax: 734-483-1592 www.pointescientific.com