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Culture Documents
PROBLEM STATEMENT:
What is the effect of temperature on the activity of enzyme amylase on starch
Apakah kesan suhu ke atas aktiviti enzim amilase ke atas ________
HYPOTHESIS:
The enzymatic reaction increases with the temperature until reaches the optimum
temperature.
Tindakbalas enzim ________ dengan suhu sehingga mencapai suhu optimum
VARIABLES:
Manipulated variable : Temperature
Suhu
Responding Variable: the time taken for the hydrolyse the starch
Masa yang diambil untuk menghidrolisiskan ________
Materials:
PROCEDURE:
KI-Preparation of materials & Apparatus
K2-Operating the CV
K3-Operating the RV
K4- Operating the MV
K5-Step to increase reliability of result accurately / precaution
5correct -3m
4-5correct 2m
1-2correct 1m
K2, K1
1. 5.0 ml of starch suspension is poured into 5 different test tube, labelled
A,B,C,D and E
5.0 ml kanji dituangkan ke dalam 5 tabung uji yang berbeza yang berlabel
A,B,C, D dan E
K2,K1
2. 2.0 ml of 5% of amylase suspension is poured into 5 different test tube
which labelled A1,B1,C1,D1 and E1
2.0 ml larutan amilase 5 % dituangkan ke dalam 5 tabung uji yang berbeza
yang dilablekan A1,B1, C1, D1 dan E1
K3,K1
3. Test tube A and A1 , B and B1, C and C1, Dand D1 , E and E1 are immersed
respectively in 5 different water baths which are kept on temperature 00C,
250C, 370C, 450C and 550C
Tabung uji A dan A1 , B dan B1, C dan C1, D dan D1 , E dan E1 direndamkan
ke dalam 5 pengukus air pada suhu 00C, 250C, 370C, 450C and 550C.
K1
4.The test tube are left for 10 minutes
Tabung uji direndamkan selama 10 minit
K1
5.A drop of iodine is poured into each groove of the white tile.
Setitis iodin dituangkan ke setiap lubang yang ada pada jubin putih.
K1,K5
6.The starch suspension in test tube A is poured into test tube A1, B to B1, C to
C1, D to D1and E to E1. A stop watch is activated
Each mixture is stir with different glass rod.
Larutan kanji di dalam abung uji A dituangkan ke dalam A1, B ke B1, C ke C1 ,
D ke D1 dan E ke E1.Jam randik diaktifkan.
Setiap campuran dikacau dengan rod kaca yang berbeza
K1
7.A drop of mixture from A1,B1,C1,D1 and E1 is dropped into the first
groove tile containing the iodine solution.
Satu titis campuran dari A1,B1,C1,D1 dan E1 dititiskn ke lubang jubin yang
pertama yang mengandungi larutan iodin
8.The iodine test is repeated every minute for ten minutes. The time taken for K4
the hydrolisis of starch to be completed is recorded
until the mixture is no longer turns blue black/ remain yellow
Ujian iodine diulang setiap satu minit untuk 10 minit. Masa untuk kanji
dihidrolisiskan dengan sempurna direkodkan apabila
Campuran itu tidak bertukar ke warna biru/ tetap berwarna kuning keperangan
K5. K1
9.The result are recorded in the table and a graph showing the rate of
enzymatic reaction,1/t against temperature is plotted.
Keputusan dicatatkan di dalam jadual dan graf yang menunjukkan kadar
tindakbalas enzim, 1/t melawan suhu diplotkan.
PRESENTATION OF DATA:
2m
Test tube
Tabung
uji
Temperatue /0C
Suhu / 0C
Rate of enzyme
reaction /1/t (s-1 )
Kadar tindakbalas
A1
0
B1
25
C1
37
D1
45
E1
55
* Temperature: at least 3
* Suhu: minima 3
1.
PROBLEM STATEMENT:
What is the effect of pH on enzyme activity?
Apakah kesan ________terhadap aktiviti enzim?
2.
HYPOTHESIS:
Pepsin works best in an acidic medium.
________ bertindak paling baik dalam medium berasid.
3.
VARIABLES:
Manipulated variable:
Responding variable:
4.
Constant variable :
APPARATUS AND MATERIALS:
5.
PROCEDURE:
1. Pour 5 ml albumen suspension into each three test tubes labeled P, Q and R.
Masukkan 5 ml ampaian albumen ke dalam tiga tabung uji berlabel P, Q
dan R masing- masing.
K1,
K2
2. Add the following solutions into each test tube according to the table below.
Masukkan larutan ke dalam setiap tabung uji mengikut jadual di bawah.
K1,
Test tube /
Tabung uji
P
Q
R
Larutan / Solution
1 ml 0.1M hydrochloric acid + 1 ml pepsin solution K4
1 ml 0.1M sodium hydroxide solution + 1 ml pepsin solution
1 ml distilled water + 1 ml pepsin solution
3. Dip a piece of pH paper into each test tube. Record the pH value.
Celupkan sehelai kertas pH ke dalam setiap tabung uji. Rekodkan nilai pH.
K1
K1,
K2
minutes.
Perhatikan warna / keadaan larutan di dalam setiap tabung uji selepas 30
minit.
6. Record the observations into a table.
Rekodkan pemerhatian ke dalam jadual.
6.
K3,
K2
K1
PRESENTATION OF DATA:
Test tube
Tabung uji
pH
3
(acidic/berasid)
9
(alkaline/ beralkali)
7
(neutral)
Q
R
PROBLEM STATEMENT :
Do different types of fruits juices contain similar amounts of vitamin C?
Adakah jus-jus buah yang berlainan jenis mengandungi kuantiti vitamin C yang sama
banyak?
2.
HYPOTHESIS :
Lime juice contains a higher concentration of vitamin C compared to pineapple juice
and orange juice.
Jus limau mengandungi kepekatan vitamin C yang lebih tinggi berbanding dengan jus
nanas dan jus oren.
3.
VARIABLES :
Manipulated variables :
Responding variables:
4.
Constant variable :
APPARATUS & MATERIALS:
Apparatus :
5.
Materials :
PROCEDURE:
K1
K2
K1
K2
K1
Masukkan picagari berisi larutan berisi asid askorbik ke dalam tiub spesimen
berisi DCPIP.
4. Add the ascorbic acid solution to the DCPIP drop by drop, stirring gently with the
syringe needle. Continue adding the ascorbic acid solution until the DCPIP
solution become colourless. Record the volume of ascorbic acid solution used.
Campurkan asid askorbik setitis demi setitis sambil mengacau larutan tersebut
dengan jarum picagari. Terus campurkan asid askorbik sehingga larutan DCPIP
menjadi tidak berwarna. Catatkan isipadu asid askorbik yang telah digunakan .
K1
K5
K4
5. Repeat steps 1 to 4 using freshly squeezed lime juice, pineapple juice and orange
juice. Each time record the volume of fruit juice required to decolourise DCPIP
solution
Ulang langkah-langkah 1 hingga 4 dengan menggunakan jus limau, nanas dan
oren segar. Catatkan isi padu jus buah yang diperlukan untuk melunturkan warna
larutan DCPIP.
K1
K3
K4
6. Tabulate the results. Calculate the percentage and then the concentration of
vitamin C in each of the fruits juices using the formulae below:
Catatkan keputusan dalam jadual. Kirakan peratus dan kepekatan vitamin C
dalam setiap jenis jus buah dengan menggunakan formula berikut:
K1,K3
X 0.1
X 0.1
PRESENTATION OF DATA:
Solution / fruit juice
Larutan /jus buah
Ascorbic acid
solution
Lime juice
Pineapple juice
Orange juice
X 1.0 mg cm
PROBLEM STATEMENT:
What is the effect of nitrogen deficiency in culture solution on the height/
growth rate of seedling?
Apakah kesan kekurangan ________mempengaruhi kadar ketinggian biji
benih?
2.
HYPOTHESIS:
In complete Knops solution, the height of seedling / the growth rate is higher
Dalam larutan Knops yang ________, ketinggian biji benih/kadar
pertumbuhan adalah lebih ________
3.
VARIABLES:
Manipulated variable : .
Responding variable :
4.
Constant variable :
APPARATUS AND MATERIAL:
Apparatus :
Materials :
5.
PROCEDURE :
K1 : Preparation Of Materials & Apparatus
K2 : Operating The CV
K3 : Operating The RV
K4 : Operating The MV
K5 : Steps To Increase Reliability Of Result Accurately/Precaution
1. Three glass jar are labelled A, B and C are prepared.
Tiga balang kaca dilabel sebagai A, B dan C disediakan.
2. In glass jar A, distilled water is fulfilled which serves as a control
K1
K1,K
experiment
Dalam balang kaca A, air suling dipenuhkan sebagai eksperimen kawalan.
K1,K
2
K1
5. Each jar is wrapped with black paper to prevent light from penetrating into
the culture solution which will cause the growth of green algae
Setiap balang kaca di bungkus dengan kertas hitam untuk mengelakkan
daripada terkena sinaran cahaya matahari yang mengganggu larutan
kultur dan boleh menyebabkan pertumbuhan alga hijau.
K1
6. Three maize seedling of the same height are chosen and put into each jars.
Tiga biji benih yang sama ketinggian dipilih dan dimasukkan ke dalam
setiap jar.
K1,K
5
7. Keep of the roots seedlings are fully immersed in each solutions. The
culture solution is aerated using an air pump to ensure the root of the
seedling obtain enough oxygen for respiration.
Pastikan akar biji benih direndam sepenuhnya dalam setiap larutan. Udara
akan dipam keluar dengan menggunakan pam udara untuk memastikan
akar menerima cukup oksigen untuk respirasi.
8. All set of apparatus are exposed to light so the seedlind are able to carry out
photosynthesis
Semua radas akan di dedahkan di bawah cahaya, maka biji benih dapat
menjalankan fotosintesis.
K1,
K5
9. The culture solution in each jar is replaced every week to ensure that the
nutrients which are supposed to be available are not depleted.
Larutan kultur pada setiap balang, akan digantikan setiap minggu untuk
memastikan kandungan nutrient mencukupi.
K5
10. After one month, seedling jar A is taken out and the final height of seedling K1
is measured by using a ruler. The growth rate of seedling is calculated and
then is recorded in a table.
Setelah sebulan, biji benih balang A, akan diambil keluar untuk merekod
6.
11. The experiment are repeated with seedling in glass jar B and glass jar C
are observed.
Eksperimen diulangi dengan menggunakan biji benih balang C dan
balang C dan perhatikan.
K1
12. Record the result in a table and a plot a bar chart showing the rate of
seedlings against the types of solution.
Rekord keputusan di dalam jadual dan plot graf bar untuk menunjukkan
kadar ketinggian pertumbuhan bergantung jenis larutan yang digunakan.
K1
PRESENTATION OF DATA :
Glass Jar
Types of solution
Jenis larutan
A
B
C
Distilled water
Air suling
Complete Knops solution
Larutan kultur knop langkap
Nitrogen deficient in culture
solution
Kesan kekurangan nitrogen
larutan kultur
PROBLEM STATEMENT:
What is the effect between the number of solid pollutants in the air from different location?
2.
Apakah kesan bilangan bahan pencemar pepejal dalam udara dari lokasi yang
berbeza?
HYPOTHESIS:
The number of solid pollutants in the air at location P is higher than location Q,
R and S.
Lokasi P mempunyai bilangan bahan pencemar pepejal dalam udara yang tinggi berbanding lokasi Q, R dan S.
3.
VARIABLE:
Manipulated variable :
Responding variable :
Constant variable
PROCEDURE:
Diagram 1//Rajah 1
1.
1. Four glass slides are cleaned and dried are label with P, Q R and S.
Empat sisip kaca dibersih dan dikeringkan di label dengan P, Q, R dan S menggunakan pen penanda.
2.
3.
2. A strip of cellophane tape with surface area of the sticky surface 3cm x 2cm facing upwards is placed on e
glass slide as shown in Diagram 1
Pita pelekat yang mempunyai luas permukaan 3cmx2cm diletakkan pada setiap sisip kaca dengan permukaan
yang melekat di sebelah atas seperti yang ditunjukkan dalam
Rajah 1.
3 Make sure your hands are clean and do not touch the sticky surface of the tape.
Tangan dipastikan dalam keadaan bersih dan tidak bersentuhan dengan permukaan pita pelekat yang melekit.
4. 4. The glass slides are placed in different location which is refer the table below:
Sisip kaca diletakkan di lokasi yang berbeza dengan merujuk jadual dibawah:
Glass slides
Location
Sisip kaca
Lokasi
Market /Pasar
5. 5. After one week, the glass slides are collected and examined under the microscope using a low power
lens.
Selepas satu minggu, sisip kaca dikumpulkan dan diperiksa satu demi satu di bawah mikroskop cahaya dengan
kanta kuasa rendah.
6. 6. Count the number of solid pollutant on glass slide P, Q, R and S and record in the table result.
7.
Kira dan rekod bilangan bahan pencemar pepejal di atas sisip kaca P, Q, R dan S dan rekod di dalam
jadual
8.
keputusan.
6. 7. Repeat the experiment to get average data and record all the data in the table result
7.
Ulang eksperimen untuk mendapat data purata dan rekod semua data di dalam jadual keputusan.
PRESENTATION OF DATA:
Glass slides
Location
Sisip kaca
Lokasi
P
Q
R
S
PROBLEM STATEMENT:
What is the level of water pollution in different sources of water?
Apakah tahap pencemaran air daripada pelbagai sumber air?
2.
HYPOTHESIS:
The more polluted the water sample which is drain water, the faster the time
taken for the methylene blue solution to decolourise.
Air yang semakin tercemar terutamanya air longkang mengambil masa yang
paling ________ untuk melunturkan warna larutan metilena biru.
3.
VARIABLES:
Manipulated variable:
Responding variable:
Constant variable :
APPARATUS & MATERIALS:
Apparatus:
Materials:
PROCEDURE:
4.
5.
P:
Drain water
Air longkang
Q: River water
Air sungai
R: Lake water
Air tasik
S: Pipe water
Air pili
T: Distilled water
Air suling
4. Using a syringe, slowly add 1ml of 0.1% methylene blue solution to the
water sample.
Dengan menggunakan picagari, tambahkan 1ml larutan metilena biru
0.1% ke dalam sampel air dengan perlahan-lahan.
5. Close the reagent bottle immediately.
Tutup botol dengan segera.
Do not shake the bottle.
Jangan goncang botol reagen.
6. Place all the bottles in a dark area.
Letakkan semua botol dalam kawasan gelap.
7. Every one hour, check for the change in the colour for every bottle.
Periksa perubahan warna untuk setiap botol pada selang masa 1 jam
.
8. Record the time taken for the methylene blue solution to turn colourless.
Rekod masa yang dimbil untuk warna larutan metilena biru menjadi
luntur.
6.
RESULTS:
Reagent
bottle
P
Q
R
S
T
Water sample
Drain water
River water
Lake water
Pipe water
Distilled water
T5
PROBLEM STATEMENT :
What is the effect of air movement on the rate of transpiration?
Apakah kesan pergerakan ________ terhadap kadar transpirasi?
HYPOTHESIS :
3.
The faster the movement of air, the greater the rate of transpiration.
Semakin ________ pergerakan udara, semakin ________kadar transpirasi.
VARIABLES :
Manipulated variable :
Pergerakan udara
Responding Variable:
Constant Variable:
4.
5.
PROCEDURE:
K1 : Preparation Of Materials & Apparatus
K2 : Operating the CV
K3 : Operating the RV
K4 : Operating the MV
K5 : Steps to increase reliability of result accurately / precaution
1. Choose a leafy shoot from a plant. Cut off the shoot with secateurs
and immediately immerse the cut end into a basin of water.
Pilih satu pucuk berdaun daripada sebatang pokok. Potong pucuk
dengan memotong ranting dan rendamkan segera hujung yang
dipotong ke dalam besen.
2.
From the cut end of the shoot, cut about 1 cm of the stem obliquely
under the water.
Daripada hujung pokok yang dipotong, potong 1 cm batang di
dalam air.
3.
4. Carefully insert the cut end of the stem into the hole in the cork of the
potometer under water. Make sure the leaves are kept out of the
water as much as possible.
Dengan berhati-hati, masukkan hujung batang ke dalam
penyumbat gabus potometer di dalam air. Pastikan daun dijauhkan
daripada air.
5.
Close the reservoir tap before you remove the apparatus from the
water so that graduated capillary tube is full.
Tutup klip air sebelum mengalirkan radas daripada air supaya tiub
kapilari dipenuhi air.
6.
Remove the apparatus from the water and set it up. The end of the
capillary tube is immersed in a beaker of water.
Alihkan radas daripada air. Hujung kapilari direndam ke dalam
bikar yang mengandungi air.
7. Wipe the leaves and the apparatus dry by using dry cloth.
Lapkan daun dan radas dengan kain kering.
PRESENTATION OF DATA:
Condition
Keadaan
Non windy
Udara
tenang
Windy
Udara
bergerak
2nd reading
Bacaan
kedua
3rd reading
Bacaan
ketiga
Average
Purata
Rate of
transpiration
(cm/minute)
Kadar
transpirasi
(cm/minit)
1.
2.
HYPOTHESIS:
The higher the light intensity, the higher the rate of transpiration of Hibiscus sp.
Semakin ________ keamatan cahaya, semakin tinggi kadar
________Hibiscus sp.
3.
VARIABLES:
Manipulated variable:
Responding variable:
Constant variable :
4.
5.
PROCEDURE:
K1: Preparation Of Materials & Apparatus
K2: Operating The CV
K3: Operating The RV
K4: Operating The MV
K5: Steps To Increase Reliability Of Result Accurately/Precaution
1. Cut a leafy shoot under water in a basin.
K1,
K5
K1
K1
K1
K5
K5
K1
K1
K1
10. Using stopwatch, record the time taken for the air bubble to move from
point A to point B.
Dengan menggunakan jam randik, rekodkanmasa yang diambil untuk
gelembung udara bergerak daripada titik A ke titik B.
K3
11. Repeat step 3 to 10 by placing the potometer under strong light intensity.
Ulangi langkah 3 ke 10 dengan meletakkan potometer tersebut di bawah
keamatan cahaya yang tinggi
K4
K2
K1
K3
6.
Rate of transpiration(cm
min-1 )
Kadar transpirasi(cm
min-1)
K5