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Vacuum Infiltration
in Plant Biotechnology:
A Promising Approach for Gene Delivery
for Production of Pharmaceutical Proteins
vtcmag@vtcmag.com
Figure 1. Vacuum agro-infiltration set up that consists of N. benthamiana leaves with Agrobacterium tumefaciens. A. tumefaciens containing
target gene construct was re-suspended in infiltration buffer and loaded into a desiccator that was connected to a vacuum pump (A) The entire
leaf system of a 6-week old plant was then submerged into the infiltration buffer (B). Agro-infiltration was achieved by applying and releasing a
vacuum through the pump [Source: Adv Tech Biol Med. 2013 Jun; 1(1): 103].
Figure 2. Expression of the green fluorescent protein (GFP) in vacuum agro-infiltrated leaves.
N. benthamiana leaves were infiltrated with A. tumefaciens harboring the GFP gene in MagnICON vectors. Post agro-infiltration leaves were photographed in a dark room under UV light
for 6 days [Source: Adv Tech Biol Med. 2013 Jun; 1(1): 103].
Figure 3. Commercial scale production of N. benthamiana plant (A) and agro-infiltration (B). [Source: Adv Tech Biol Med. 2013 Jun; 1(1): 103
and Kentucky Bioprocessing, LLC].
vtcmag@vtcmag.com