Reagents

1.

Conditioning reagent: - Mix 50-ml glycerol with a solution containing 30 ml.

Concentrated HCL. 300 ml distilled water, 100 ml. 95% ethyl or isopropyl alcohol and
75 g. NaCI.

2.

Barium chlorides 20 30 mesh.

3.

Standard sulphate solution: - Dissolve 147.9 mg. Anhydrous Na2SO4 and dilute to 100
ml.

1 ml = 100 ug SO4
Procedure
1.

Take suitable vol. of sample in 250-ml conical flask and dilute to 100 ml.

2.

Add 5 ml. Conditioning reagent accurately. Mix well

3.

Keep the flask constantly stirred with the help of stirrer. Add BaCl2 crystals while
strring. Continue stirring for 1 min. after addition of BaCl2.

4.

Measure the turbidity developed after every 30 sec. For 4 min. on colorimeter at 420
nm. after addition of BalCl2.

5.

Prepare standard curve by carrying standard sulphate solution through entire

procedure. Space standards at 5-mg/l increment in the 0 to 40-mg/l range.

6.

Read mg SO4 present in the sample from the standard curve.

Mg/l SO4 = mg SO4 x 1000 / ml sample