EXPERIMENT 4: REDOX TITRATION ASCORBIC ACID

Part A: A Primary Standard 0.01 M Potassium Iodate, KIO3, Solution

1. 0.5 g of KIO3 on weighing paper 2. Transfer to volumetric flask, dissolve &

dilute to the mark

3. Calculate & record M of KIO3 soln.

Part B: A Standard 0.1 M Na2S2O3 Solution

6g Na2S2O35H2O
+
250 mL

1. Dissolve 6g Na2S2O35H2O with 2. Fill burette with Na2S2O3 soln, & record initial v.

distilled water, dilute to 250 mL. Stir.

25 mL standard KIO3 soln

+
1 g of KI
+
5 mL H2SO4
+
0.1 g NaHCO3

3. Pipette 25 mL standard KIO3 soln into Erlenmeyer flask.

Add 1 g of solid KI.

Add 5 mL of 0.5 M H2SO4 & 0.1 g of NaHCO3.

EXPERIMENT 4: REDOX TITRATION ASCORBIC ACID

Red brown soln Pale yellow, Add 2 mL starch, Stir until no blue colour.

4. Immediately begin titrating with Na2S2O3 soln. When the red-brown solution changes to a pale
yellow colour, add 2 mL of starch solution. Stirring constantly, continue titrating slowly until the blue
colour disappears.

5. Repeat 2x.

6. Calculate and record the molar concentration of the Na2S2O3 solution.

Part C: Sample Preparation

(a) Vitamin C tablet
1. Read label, determine mass of vitamin C in each tablet. Measure the fraction of the total mass of a
tablet that corresponds to 100 mg of ascorbic acid.

Vit C
+
40 mL H2SO4
+
0.5 g NaHCO3

2. Dissolve vit C in Erlenmeyer flask with 40 mL of 0.5 M H2SO4, and add 0.5 g NaHCO3.

3. Proceed immediately to Part D.

(b) Fresh fruit sample

1. Filter about 120 mL of freshly squeezed 2. Measure the mass of a clean, dry 250 mL
juice through several layers of muslin. Erlenmeyer flask.
EXPERIMENT 4: REDOX TITRATION ASCORBIC ACID

100 mL filtered juice

+
40 mL H2SO4
+
0.5 g NaHCO3

3. Add 100 mL filtered juice & determine the mass.

Add 40 mL of 0.5 M H2SO4, and add 0.5 g NaHCO3.

4. Proceed immediately to Part D.

Part D: Vitamin C analysis

25 mL standard KIO3 soln
+
0.1 g NaHCO3

sample solution from Part C

5 mL H2SO4
+
0.1 g NaHCO3

1. Pipette 25.0 mL standard KIO3 solution (from Part A) into sample solution from Part C.
Add 1 g KI.
Add 5mL 0.5 M H2SO4 and 0.1 g NaHCO3.

Red brown soln Pale yellow, Add 2 mL starch, Stir until no blue colour.

2. Titrate excess I3 in sample with standard Na2S2O3 solution as described in Part B.4.

3. Read & record the final burette reading. Repeat 2x. Calculate % of ascorbic acid in the sample.