ESCO

Worldwide Headquarters
Esco Micro Pte. Ltd.
21 Changi South Street 1, Singapore 486777
Tel: +65 65420833 Fax: +65 65426920
The Esco Group of Companies Email: biotech@escoglobal.com
WWW Site: http://biotech.escoglobal.com

Labculture Vertical Laminar Flow Cabinets

Factory Testing and Commissioning Report
Model: LVC-4A1. Serial Number: ____________________

1. Airflow Velocity and Uniformity Test

Test Points on Filter Face

Pt 1 _____ m/s Pt 3 _____ m/s Pt 5 _____ m/s Pt 7 _____ m/s A calibrated airflow meter was set up within the
interior of the cabinet to measure filter airflow at
a distance of 15 cm / 6 inches from the filter
face. The filter face was divided into a suitable
number of grids. The grids are 15 cm from the
walls and 30 cm cartesian apart. Prior to this
test, the cabinet blower system was factory
Pt 2 _____ m/s Pt 4 _____ m/s Pt 6 _____ m/s Pt 8 _____ m/s adjusted after production.

<---- Approx 1.2m ---->

Left Side Right Side

Average Velocity : ____________________ m/s

Maximum Deviation : ____________________ %

Final Conclusion : Pass / Fail

Acceptance Criteria : Average filter face velocity of 0.4 0.5m/s or 80 100 fpm, with no single value
deviating more than 20% from the calculated average.

Testing was performed:

In accordance with the following IEST (Institute of Environmental Sciences and Technology) contamination
control document: IEST-RP-CC002.2 Recommended Practice for Unidirectional Flow Clean Air Devices
Section 6.1

2. Particle Count / Product Protection Test

The counting was evaluated in accordance with the cleanliness requirements of the following standards:
Class 3 as per ISO 14644 Class 1 / M.1.5 as per US Federal Standard 209E
Class 0.035 as per Australian Standard AS 1386 Class 1 as per German Standard VDI 2083
Class C as per British Standard BS 5295 Class 40 as per French Standard AFNOR X44101
Class 3 as per Japanese Standard JIS B9920 Class M100 as per Korean Standard 27030.1

Report page 1 of 4
ESCO
Worldwide Headquarters
Esco Micro Pte. Ltd.
21 Changi South Street 1, Singapore 486777
Tel: +65 65420833 Fax: +65 65426920
The Esco Group of Companies Email: biotech@escoglobal.com
WWW Site: http://biotech.escoglobal.com

Labculture Vertical Laminar Flow Cabinets

Factory Testing and Commissioning Report
Model: LVC-4A1. Serial Number: ____________________

Test Points on Filter Face

An airborne particle counter was set up within

the interior of the cabinet to count 0.1 micron
Pt 1 _______ Pt 3 _______ sized particles. The filter face was divided into a
suitable number of grids. Cumulative particle
count for a duration of 1 minute was taken at
the centre of each imaginary grid, taking air
sample of 1 cu ft. Finally, from the readings
obtained, an average value was calculated.
Pt 2 _______ Pt 4 _______ Original particle counter printouts are not
included with this report but are available, on
request, for inspection, from Esco.

<---- Approx 1.2m ---->

Left Side Right Side

Average Count : ____________________ Pass / Fail

Counting status : As-built

Acceptance Criteria : Average count of less than 35 particles per cubic foot of air

Testing was performed:

a. In accordance with the cleanliness requirements of the standards above.

b. In accordance with the following IEST (Institute of Environmental Sciences and Technology)
contamination control documents: IEST-RP-CC002.2 Recommended Practice for Unidirectional Flow
Clean Air Devices Section 6.6 and IEST-G-CC1001 Technical Guide for Counting Airborne Particles
for Classification and Monitoring of Cleanrooms and Clean Zones

3. Natural Aerosol Challenge Filter Leak Scan (PAO Equivalent)

With the particle counter set to monitor particles on 0.1 microns, the counter probe was slowly moved over
the entire surface of the filter (including areas surround the gasket). Any spikes in the instantaneous
particle count (which could represent a possible filter leak) were recorded. The test was performed with the
cabinet sited in a normal room environment.

Acceptance Criteria : No filter leaks should be detected *

Results : Pass / Fail

* In accordance with the requirements of the following standards:

IEST-RP-CC001.3 Recommended Practice for HEPA and ULPA Filters
EN 1822 High Efficiency Air Filters (HEPA and ULPA)

Report page 2 of 4
ESCO
Worldwide Headquarters
Esco Micro Pte. Ltd.
21 Changi South Street 1, Singapore 486777
Tel: +65 65420833 Fax: +65 65426920
The Esco Group of Companies Email: biotech@escoglobal.com
WWW Site: http://biotech.escoglobal.com

Labculture Vertical Laminar Flow Cabinets

Factory Testing and Commissioning Report
Model: LVC-4A1. Serial Number: ____________________

Testing was performed:

a. In accordance with the requirements of the standards above.

b. In accordance with the following IEST (Institute of Environmental Sciences and Technology)
contamination control documents: IEST-RP-CC034.1 Recommended Practice for HEPA and ULPA
Filter Leak Tests, IEST-RP-CC021.1 Recommended Practice for Testing HEPA and ULPA Filter
Media and IEST-RP-CC007.1 Recommended Practice for Testing ULPA Filters.

4. Light Intensity Test

A light intensity meter was used to measure light

intensity at work surface level within the cabinet. The
work surface area was divided into a suitable number
Pt 1 ______ lux Pt 2 ______ lux
of grids. One reading was taken at the centre of each
grid.

Acceptance Criteria : More than 800 lux*

<---- Approx 1.2m ---->
Average Reading : ____________ lux Pass / Fail
Left Side Right Side
*) Based on IEST-RP-CC002.2 Section 6.10

5. Noise Level Test

The cabinet was isolated in a noise-controlled environment and a noise level meter was set up at a distance
of 30 cm in front of the cabinet and 38 cm above the work surface. With the cabinet running under normal
parameters, the instrument was used to obtain a noise level of the cabinet during normal operation.

Results: : __________ dBA Pass / Fail

Acceptance Criteria : Less than 67 dBA (Based on IEST-RP-CC002.2 Section 6.11)

6. Vibration Test

Actual vibration (cabinet on) : __________________________________ m RMS amplitude

Background vibration (cabinet off) : __________________________________ m RMS amplitude

Net vibration (actual minus backgd.) : __________________________________ m RMS amplitude

Acceptance : Maximum cabinet vibration (net value) should be less than 0.5 x 10-5 m RMS amplitude

Result : Pass / Fail

Report page 3 of 4
ESCO
Worldwide Headquarters
Esco Micro Pte. Ltd.
21 Changi South Street 1, Singapore 486777
Tel: +65 65420833 Fax: +65 65426920
The Esco Group of Companies Email: biotech@escoglobal.com
WWW Site: http://biotech.escoglobal.com

Labculture Vertical Laminar Flow Cabinets

Factory Testing and Commissioning Report
Model: LVC-4A1. Serial Number: ____________________

Based on the above tests, the overall performance of the cabinet was determined to be:

In compliance with Esco specifications and the applicable international requirements.

Date : ____________________________________

Test Engineer : ____________________________________

Recommended re-certification is 12 months from initial date of operation

Report page 4 of 4