Professional Documents
Culture Documents
TypeB2LaminarFlow
BiologicalSafetyCabinet
Models
NU430400/600
Bench/Console
Operation&MaintenanceManual
September2014
(Series66)
Revision2
ManufacturedBy:
NuAire,Inc.
2100FernbrookLane
Plymouth,MN55447
TollFree:18003283352
InMinnesota:(763)5531270
Fax:(763)5530459
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Congratulations!
YouhavejustpurchasedoneofthefinestLaminarFlowBiologicalSafetyCabinetsavailable.Withpropercare,
maintenance(certification),andlaboratoryprocedure,thiscabinetwillgiveyouyearsofproductandpersonnelprotectionfrom
particulatecontaminantsasprescribedinNSF/ANSI49.Pleasereadthismanualcarefullytofamiliarizeyouwithproperinstallation,
maintenanceandoperationofthecabinet.Otherreferenceandguidelinematerialsareavailablethroughthefollowingwebsites.
www.hcsc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absacanada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.osha.gov/dts/osta/
www.nuaire.com
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ABOUTTHISOPERATION&MAINTENANCEMANUAL
Theinformationcontainedinthismanualisintendedtoreflectourcurrentproductionstandardconfigurationmodelalong
withthemorefrequentlypurchasedoptions.Anyuniqueadditions/modifications/shopdrawingsareappendedinthebackflapof
thismanual,alongwithanymodificationsand/oradditionstoproceduresasoutlinedinthismanual.Acopyoftheoriginalfactory
testreportisalsoappendedtothismanual.Incasethismanualand/ortestreportislostormisplaced,NuAireretainsacopyinour
files.AreplacementcopycanbeobtainedbycallingorwritingNuAire,Inc.statingthemodelnumberandserialnumberandabrief
descriptionoftheinformationdesired.
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LabgardESEnergySaverClassII,TypeB2LaminarFlowBiologicalSafetyCabinet
ModelsNU430400/600Operation&MaintenanceManual
TABLEOFCONTENTS
SectionNo.1...........................................................GeneralInformation
SectionNo.2...........................................................Models&Features
SectionNo.3...........................................................Warranty
SectionNo.4...........................................................Shipments
SectionNo.5...........................................................InstallationInstructions
5.1.................................................................Location
5.2.................................................................SetupInstructions
5.3.................................................................Exhaust/SupplyAirChecks
5.4.................................................................CertificationTestingMethodsandEquipment
SectionNo.6...........................................................OperatingtheNU430
6.1.................................................................BiologicalSafetyCabinetControl
6.2.................................................................OperatingGuidelines
6.3.................................................................OperatingSequence
6.4.................................................................Ergonomics
6.5.................................................................CleaningProcedures
6.6.................................................................HazardousDrugDecontaminationProcedure
SectionNo.7...........................................................GeneralMaintenance
7.1.................................................................Decontamination
7.2.................................................................FluorescentLampBulbReplacement
7.3.................................................................HEPAFilter/MotorReplacement
7.4.................................................................SlidingWindowReplacement&Adjustment
7.5.................................................................AirflowControlSystemSetup&Calibration
7.6.................................................................HEPAFilterLeakTest
7.7.................................................................AirflowSmokePatternTest
7.8.................................................................SiteInstallationAssessmentTests
7.9.................................................................CleanlinessClassificationTestforPharmacyApplication
7.10...............................................................MainControlBoardDescription&Replacement
7.11...............................................................DigitalAirflowSensorDescription&Replacement
SectionNo.8...........................................................ErrorIndicators,Troubleshooting,OptionDiagnostics&
AirflowSensorPerformanceVerification
SectionNo.9...........................................................RemoteContacts
SectionNo.10.........................................................OptionalEquipment
10.1.................................................................UltravioletLight
SectionNo.11.........................................................Electrical/EnvironmentalRequirements
SectionNo.12.........................................................DisposalandRecycle
Insert..........................................................................ReplacementPartsList
MANUALDRAWINGS
ACD12153....................................................NU430AirflowSchematic
BCD15501.....................................................NU430400SpecificationDrawing
BCD15502.....................................................NU430600SpecificationDrawing
BCD11815.....................................................DrainValveInstallation
BCD05572.....................................................ButterflyValveInstallation
BCD05660.....................................................BagIn/BagOutProcedure
ASSEMBLYDRAWINGS
BCD05147.....................................................BaseStandAssembly
BCD05146.....................................................BaseStandStorageCabinetAssembly
BCD11817.....................................................ControlCenter&FrontDecorativePanelAssembly
BCD11818.....................................................SlidingWindowAssembly&Adjustment
BCD14185.....................................................BaseCabinetAssembly
ELECTRICALSCHEMATICS
BCD16606(Sheets12)................................NU430400/600ElectricalSchematic
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LabgardESEnergySaverClassII,
TypeB2LaminarFlow
BiologicalSafetyCabinet
Models
NU430400/600
MANUFACTUREDBY:
NuAire,Inc.Plymouth,Minnesota,U.S.A.
1.0 GeneralInformation
1.1Description
TheLABGARDESModelNU430LaminarFlowBiologicalSafetyCabinet(LFBSC)isabench/tabletopmodel,optionally
availablewithabasesupportstand,foroperationasaconsolemodel.TheLABGARDESmodelNU430utilizesanEnergy
SaverDCECMmotoroptimallydeterminedforwardcurvedfanforeachmodelsize/widthtomaximizebothenergy
efficiencyandfilterloadingcapacity.TheEnergySaverECMmotoriscontrolledtoairflowsetpointsviaasolidstateDC
motorcontrollerwithdigitaldualthermistorairflowsensorsthatprovideanautomaticcompensation(constantvolume
control)forbothfilterloadingandlinevoltagevariances.
TheLaminarFlowBiologicalSafetyCabinet,(LFBSC)isaproductresultingfromthedevelopmentofthe"laminarflow"
principleandtheapplicationofenvironmentalcontrolsasrequiredinthefieldofbiologicalresearchorchemical
containment.TheLFBSC,whenusedwithpropertechnique,isaneffectivelaboratoryaidinobtainingtheoptimumcontrol
overproductqualitywhilereducingthepotentialforexposureofbothproductandpersonneltoairbornebiologicalor
particulatechemicalagentsinlowtomoderateriskhazardresearchanddrugpreparationorproductoperations,as
prescribedbytheCenterforDiseaseControl(CDC)Atlanta,Georgia.
TheNU430BenchLFBSCmeetstherequirementsofaClassII,TypeB2sincethecabinetconformstothefollowing
requirements:
- Maintainaminimumaverageinflowvelocityof100fpm(0.51m/s)throughtheworkaccessopening;
- HaveHEPAfiltereddownflowairdrawnfromthelaboratoryortheoutsideair(i.e.downflowairisnotrecirculated
fromthecabinetexhaustair;
- Exhaustallinflowanddownflowairtotheatmospherethroughahardconnectiontothefacilityexhaustsystemafter
filtrationthroughaHEPAfilterwithoutrecirculationinthecabinetorreturntothelaboratory;
- Haveallcontaminatedductsandplenumsundernegativepressureorsurroundedbydirectlyexhausted
(nonrecirculatedthroughtheworkarea)negativepressureductsandplenums.
TypeB2cabinetsmaybeusedforworkwithnonflammableorexplosivevolatilechemicalsandradionuclidesrequired
asadjunctstomicrobiologicalstudies.
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1.2SafetyInstructions
ThesesafetyinstructionsdescribethesafetyfeaturesoftheLABGARDESModelNU430LFBSC.
Thesafetycabinethasbeenmanufacturedusingthelatesttechnologicaldevelopmentsandhasbeenthoroughlytested
beforedelivery.However,thecabinetmaypresentpotentialhazardsifitisnotinstalledandusedasinstructedforits
intendedpurposeoroutsideofoperatingparameters.Therefore,thefollowingproceduresmustalwaysbeobserved:
Thesafetycabinetmustbeoperatedonlybytrainedandauthorizedpersonnel.
Foranyoperationofthiscabinet,theoperatormustprepareclearandconcisewritteninstructionsforoperating
andcleaning,utilizingapplicablesafetydatasheets,planthygieneguidelines,andtechnicalregulations,in
particular.
o Whichdecontaminationmeasuresaretobeappliedforthecabinetandaccessories?
o Whichprotectivemeasuresapplywhilespecificagentsareused?
o Whichmeasuresaretobetakeninthecaseofanaccident?
Repairstothedevicemustbecarriedoutonlybytrainedandauthorizedexpertpersonnel.
Keeptheseoperatinginstructionsclosetothecabinetsothatsafetyinstructionsandimportantinformationare
alwaysaccessible.
Shouldyouencounterproblemsthatarenotdetailedadequatelyintheoperatinginstructions,pleasecontactyour
NuAireRepresentativeofNuAiretechnicalServices.
1.3 ExplanationofSymbols
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2.0 Models&Features
ThemodelNU430,LABGARDESClassII,TypeB2LaminarFlowBiologicalSafetyCabinetismanufacturedintwosizes:
4ft.(1.2m)and6ft.(1.8m).
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3.0 Warranty
NuAire,Inc.warrantsClassII,TypeB2w/ECMMotor(LABGARDES)thatitwillrepairF.O.B.itsfactoryorfurnishwithout
chargeF.O.B.itsfactoryasimilarparttoreplaceanymaterialincludingsupplyHEPAfilter(excludingexhaustHEPAfilter)in
itsequipmentwithin60monthsafterthedateofsaleifprovedtothesatisfactionofthecompanytohavebeendefective
atthetimeitwassoldprovidedthatallpartsclaimeddefectiveshallbereturned,properlyidentifiedtothecompanyatits
factory,chargesprepaid.Factoryinstalledequipmentoraccessoriesarewarrantedonlytotheextentguaranteedbythe
originalmanufacturer,andthiswarrantyshallnotapplytoanyportionoftheequipmentmodifiedbytheuser.Claims
underthiswarrantyshouldbedirectedtoNuAire,Inc.settingforthindetailthenatureofthedefect,thedateoftheinitial
installationandtheserialandmodelnumberoftheequipment.
ThiswarrantyshallnotapplytoanyNuAireproductorpartthereofwhichhasbeensubjecttomisuse,abuse,accident,
shippingdamage,improperinstallationorservice,ordamagebyfire,floodoractsofGod.Iftheserialnumberofthis
productisaltered,removedordefacedastobeillegible,theWarrantyshallbenullandvoidinitsentirety.
Thewarrantyisforthesolebenefitoftheoriginalpurchaserandisnotassignableortransferable.Priortoreturningany
item,foranyreason,contactNuAireforaReturnAuthorizationNumber.Thisnumbermustaccompanyallreturns.Any
productshippedtoNuAirewithoutthisnumberwillbereturnedrefusedshipmentorcollectfreight.
4.0 Shipments
NuAiretakeseveryreasonableprecautiontoassurethatyourLABGARDEScabinetarriveswithoutdamage.Motorcarriers
arecarefullyselectedandshippingcartonshavebeenspeciallydesignedtoinsureyourpurchase.However,damagecan
occurinanyshipmentandthefollowingoutlinesthestepsyoushouldtakeonreceiptofaNuAireLABGARDEScabinetto
besurethatifdamagehasoccurred,theproperclaimsandactionsaretakenimmediately.
4.1 DamagedShipments
4.1.1 Termsarefactory,unlessstatedotherwise.Therefore,itisimportanttocheckeachshipmentbeforeacceptance.
4.1.2 Ifthereisvisibledamage,thematerialcanbeacceptedafterthedrivermakesanotationontheconsignee'scopy
ofthefreightbill.Thenaninspectionmustbemadetoverifytheclaimagainstthecarrier.Thisinspectionisthe
basisofyourfilingtheclaimagainstthecarrier.
4.1.3 IfconcealeddamageisfounditisabsolutelynecessarytoNOTIFYTHEFREIGHTAGENTATONCEandrequestan
inspection.Withoutthisinspection,thetransportationcompanymaynotacceptaclaimforlossordamage.Ifthe
carrierwillnotperformtheinspection,anaffidavitmustbepreparedstatingthathewascontactedonacertain
dateandthathefailedtocomplywiththerequest.Thisalongwithotherpapersinthecustomer'spossessionwill
supporttheclaim.
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5.0 InstallationInstructions
5.1 Location
Withinthelaboratory,pharmacy,etc.,theideallocationofthebiologicalsafetycabinetisawayfrompersonneltraffic
lanes,airvents(inorout),doorsand/oranyothersourceofdisruptiveaircurrents.
Ifdraftsorotherdisruptiveaircurrentsexceedtheinflowvelocityofthecabinetthroughtheaccessopening,thepotential
existsforcontaminatedairtoexitorentertheworkzoneareaofthecabinet.Itdependsontheseverityoftheaircurrent.
REMEMBER:ABIOLOGICALSAFETYCABINETISNOSUBSTITUTEFORGOODLABORATORYTECHNIQUE.
Wherespacepermits,aclear6inch(152mm)areashouldbepermittedoneachsideofthecabinetformaintenance
purposes.Theelectricaloutletintowhichthecabinetisconnectedshouldbereadilyaccessibleformaintenancepurposes.
Donotpositionthecabinettopreventaccesstothepowercord.Thepowercordplugservesasthedisconnectand
shouldremainreadilyaccessible.Iftheoutletisinaccessible,suchasaconduit(hardwired)connection,thenanappropriate
warninglabelshouldbeappliednearthecabinet'son/offswitch,toindicatethecircuitbreakeronthepowerdistribution
paneltobeused.
Morethananyotherbiologicalsafetycabinet,theNU430requirescarefulsiteplanningandpreparation,duetothetotal
exhaustnatureofthecabinet.Propersizingoftheexhaustandmakeupsupplysystemsarecriticaltothesuccessful
installationofthecabinet.Inaddition,thecabinetprovidesforthechoiceofmakeupairforthesupply(downflow)air.
Thefollowingareairflowrequirementsbasedonconcurrentbalancevalues*.
*ConcurrentBalanceValueisdeterminedbyaducttraversemeasurementmethodasspecifiedinASHRAEStandard111at
itsnominalsetpointcalibratedusingtheprimaryDIMmethodandcapturehoodremoved.Thesevaluesshallbeusedfor
designandbalanceexhaust/supplyHVACrequirements.
**Exhaustairvolumeatnegative1.7inches(43mm)w.g.forNU430400and
1.8inches(46mm)w.g.forNU430600.
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5.2 SetUpInstructions
Removeoutershippingprotection(cartonorcrating).Thecabinetisfastenedtothebaseskidanditisusuallythebest
proceduretoleavetheskidinplaceuntilthecabinetislocatedinitsapproximatepositiontofacilitateeaseinhandling.
Itcanthenberemovedfromtheskidbyremovingthebandingholdingthecabinettotheskid.Itmaybenecessaryto
removetheControlCenterinordertogainpassagethroughadoorway.Itmayeasilyberemovedbyfollowingthe
instructionsondrawingBCD11817.
Itisrecommendedthatnolessthantwopeoplearepresentusinga
! CAUTION liftingsystemforplacementofthecabinetontothebasestand.
Itisnotrecommendedtomanuallyliftthecabinetontothebasestand.
5.2.1 BaseStandAssembly
ThebasestandisshippedknockeddowninaseparatecartonandisassembledperdrawingBCD05147if
accompaniedwiththecabinet.Removethebandingholdingthecabinettothebaseskid.Liftthecabinetfrom
thebaseskidandplaceonthefloor.Nowliftthecabinetontopofthebaseandboltthebasestandtothecabinet
usingtwo3/8"16x3/4"boltsandwashersprovidedforthefrontbasestandtabsandtwo1/4"acornnutsfor
therearweldstuds.Placethecabinetinitsdesiredlocation.
Thebasestandstoragecabinetswillusuallybeshippedaccordingtocustomerrequirements.Ifitisshipped
unassembled,itcanbeassembledperdrawingBCD05146.Itisrecommendedthattheupperandlowerbase
standbracesbeinstalledfirst,thentherearandbottompanels(theendpanelsarealwaysprefastened).Once
assembled,fastenthecabinetpertheaboveinstructions.
Removethecapprotectingthedrainvalvethreadsandinstallthedrainvalve,onthebottomrightfrontofthe
cabinetusingLoctite242furnishedtothethreadsandrotatethevalvebodyuntilitissecure(SeeBCD11815).
5.2.2 Leveling
Usingalevelplacedontheworktray,adjusttheleglevelers,first,endtoendthenfronttoback.
TheNSFapprovedleglevelersprovidea3/4"(20mm)adjustment.
5.2.3 BenchInstallation(BCD11815)
Placethecabinetonthebenchwithapproximatelya2"(51mm)overhangclearanceforinstallationofthedrain
valve.Ifthedrainvalveisnotdesired,capwith3/8NPTfittingandplacethecabinetinitsdesiredlocationand
usingRTVcaulk,sealallaroundthebaseofthecabinetandthebench.Thisprovidesatightsealtopreventbench
spillsfrommigratingunderthecabinet.
Ifadrainvalveisdesired,(NOTE,CHECKWITHYOURSAFETYPERSONNELFORREQULATORYREQUIREMENTS(i.e.
LOCKINGTYPE)OFDRAINVALVEINSTALLATION)removethehandlefromthevalvestemtogainclearancefor
valvebodyrotation.AddLoctite242(furnished)tothethreadsandrotatevalvebodyuntilsecure,withthevalve
stem(forhandle)ontheleftside.Reinstallhandletovalvestem.Adjustthecabinetonbenchtoprovidea
11/2"(38mm)overhangandsealtheinterfaceofthebenchandcabinet,usingRTVcaulkasabove.
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5.2.4 GasService
NuAiredoesn'trecommendtheuseofnaturalgaswithintheLFBSC,butifgasserviceisdeterminedtobe
necessaryfortheapplication,appropriatesafetymeasuresmusttakeplace.AllNuAireLFBSC'shaveprecautionary
warninglabelsthatsaythefollowing:
Useofexplosiveorflammablesubstancesinthiscabinet
CAUTION
! shouldbeevaluatedbyyourappropriatesafetypersonnel.
Oncetheappropriatesafetypersonnelhavemadethedetermination,theapplicationofnaturalgasmust
beperformedinaccordancetonational,stateandlocalcodes.ITISALSOSTRONGLYRECOMMENDEDTHATAN
EMERGENCYGASSHUTOFFVALVEBEPLACEDJUSTOUTSIDETHELFBSCONTHEGASSUPPLYLINE.
AllNuAireLFBSC'smeetthesafetyrequirementsofULandCSAforLaboratoryEquipment.Tocomplywiththese
safetyrequirements,NuAireusesonlycertifiedgasvalves.Inaddition,ifexternalpipingisrequired,onlyblack
pipeisusedforthisapplication.
AspreviouslystatedNuAiredoesn'trecommendtheuseofnaturalgaswithintheLFBSCandASSUMESNO
RESPONSIBILITYFORITSUSE.USEATYOUROWNRISK.TheBunsenburnerflamewithintheLFBSCnotonly
contributestoheatbuildup;isalsodisruptsthelaminarairstream,whichmustbemaintainedformaximum
efficiency.IFTHEPROCEDUREDEMANDSUSEOFAFLAME,ABUNSENBURNERWITHONDEMANDIGNITIONIS
STRONGLYRECOMMENDED.DONOTUSECONSTANTFLAMEGASBURNERS.Duringuse,theBunsenburner
shouldbeplacedtotherearoftheworkspacewhereresultingairturbulencewillhaveaminimaleffect.
5.2.5 PlumbingServices
Serviceballvalveswiththetypeofservicespecifiedbytheremovablebuttononthehandlearelocatedinthe
workzone.Theserviceballvalvesarenotrecommendedforpressureover75p.s.i.(5.2BAR).Reducingvalves
shouldbeinstalledexternaltothecabinetifnecessary.Serviceballvalvesshouldneverbeusedforflammable
gassesoroxygenservice.Aspecialneedlevalveforoxygenserviceorcertifiedvalveisrequiredandavailableupon
request.
Externalconnectionisto3/8inchNPTcouplingintheinnersidewalls.Connectiontoplantutilitiesshouldbe
madewithpropermaterialsfortheindividualserviceandaccordingtoNationaland/orLocalcodes.Observeall
labelspertainingtothetypeofserviceandoperatingpressure.
Remotecontrolledneedlevalveplumbingfixturescanbeoptionallyprovidedwithintheinteriorsidewalls.Control
handlesarelocatedexternallyontheverticalairfoil.Serviceoutletswithintheinteriorhaveserratedtapered
fittingsdesignedforhoseconnectionswiththeremotecontrolledneedlevalveplumbingfixtures.NuAireprovides
forrear,bottom,ortopconnectionsofplumbingservicestoplantutilities.Connectionfromtheneedlevalve
assemblytotheweldedexitcouplingisaccomplishedwiththesupplied3/8inchsoftcoppertubingasstandard
(alternativematerialstomeetlocalcodesareavailableuponrequest).Theneedlevalvesarenotrecommended
forworkingpressureinexcessof125p.s.i.(8.6BAR).
5.2.6 ElectricalServices
TheNU430seriesBiologicalSafetyCabinetsmaybe"hardwired"(optional)orpluggedintoanoutletwith
protectiveearthingconnectionwiththestandardpowercord.Thecabinetrequires115VAC,60Hzsinglephase
(correctratingvariespercabinetsize,referenceElectrical/EnvironmentalRequirements).Itisrecommendedthat
powertothecabinet,whetherhardwiredorplugconnected,beonitsownbranchcircuit,protectedwithacircuit
breakeratthedistributionpanelnearthecabinet.Asurgeprotectorisstronglyrecommendedifyouare
experiencingpowerrelatedfaults.
PLEASENOTETHISCABINETCONTAINSELECTRONICBALLASTSFORTHEFLUORESCENTLIGHTING.ELECTRONIC
BALLASTSOPERATEWITHHIGHINRUSHCURRENT.ITISNOTRECOMMENDEDTOUSETHISPRODUCTWITH
GROUNDFAULTCIRCUITINTERRUPTERS(GFCI'S)BECAUSETHEBALLASTSMAYCAUSETHEGFCITOTRIP.
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5.2.7 Exhaust/SupplyDuctInstallationGuidelines
Theexhaust/supplysystemsmustprovideconditionssimilartothatunderwhichthecabinetwascertifiedtomeet
itsstatedperformance.Thefollowingguidelinesshouldbeobservedwheninstallingexhaust/supplyairductwork
foreitherexistingplantexhaustsystems,oranewexhaustsystem.
1. Adequateroommakeupairinflowtoreplaceexhaustedair.Airdiffusionrateisnottoexceedvelocityof
100LFPM(.51m/s)tominimizedisruptiveaircurrents.Iflaboratoryistobepressurized,follow
guidelinesinANSI/AIHAZ9.5LaboratoryVentilation.
RoomMakeUpAirRequirements(CFM/CMH)UtilizingConcurrentBalanceValues
Model WithSupplyDuct WithoutSupplyDuct
NU430400 345/586 785/1334
NU430600 531/902 1250/2124
2. Adequateplantexhaustsystemcapability.Theexhaustsystemisusuallyadequateifitcanprovidethe
ratedexhaustflowandstaticpressure.
3. Allductlossesmustbeconsideredandaddedtothecabinetlossinselectingtheexhaustblower,fora
newexhaustsystem(i.e.ductdiameter,lengthandnumberofelbows,etc.).
4. Adequatesupplyaircapability(ifused).Thesupplyairsystemisusuallyadequateifitcanprovidethe
ratedsupplyairat0.0incheswatergaugepositive.
5. Thesupplyairmustbeinterlockedwiththeoperationofthecabinet'sinternaldownflowblowerinorder
topreventdownflowairfrombeingforcedoutofthefrontofthecabinetwhenpoweredofforduring
nightsetbackconditions.NuAireprovidesfanrelaycontactsforthispurpose.
6. Allductworkshouldbesecurelyanchoredtothebuildingconstructioninmannertobefreefrom
vibrationandswayingunderallconditionsofoperations.
7. SheetmetalgaugesandseamsshouldbeinaccordancewiththecurrenteditionoftheASHRAEguide.
Aminimumof20gaugeforroundductisrequiredtopreventductcollapseduetohighstaticpressure
conditions(squareductwillrequireheaviergaugematerial).
8. Allductworkshouldbemaintainedatanegativepressurewithinthebuilding(i.e.externallylocated
exhaustblower).
9. Theexhaustblowerandductworkshouldbeasealedsystemthatcanhold2.0inches(51mm)w.g.
pressurefor30minuteswithnomorethana10%dropinpressure,andbeproperlyventedtothe
atmospheretodisperseexhaustedair.
10. Alocalmanualexhaustflowdamper(NuAireModelNU940)shouldbereadilyaccessible(eitherdirectly
mountedontheLFBSCexhaustcollarorjustabovetheLFBSC)forthemaintenancetechnician/certifierto
allowtheLFBSCtobesealedfordecontaminationpurposes.IfaConstantAirVolume(CAV)valveis
locatedabovetheLFBSC,anyexhaustflowadjustmentsshouldbemadetotheCAVvalveleavingthe
manualexhaustdamperinafullopenposition.
11. Itisrecommendedthatthecabinetoperationbeinterlockedwiththeexhaustblower.Fanrelaycontacts
areprovidedforthispurpose.However,itisalsorecommendedtohaveamanualexhaustoverride
switchnearthecabinetforcertificationandservice.Formultigangedsystems,thisswitchcouldbeused
tointerfacewithaBuildingAutomatedSystem(BAS).
12. ItisrecommendedthatwhenusingtheNU951012automaticbutterflyvalve,thesystemairvolume
mustremainwithintenpercentofthegivennominalsetpointvolumetooptimizethemeasurementand
controlperformance.
13. Itisnotrecommendedtohardconnect(i.e.weld)theexhaustconnectiontothecabinet.Thismay
damagetheexhaustfilterand/orthebutterflyvalve(ifpresent).Asiliconsleeve(NuAirePartNo.NU
940001),bandedbetweenthecabinet'sexhaustductandtheplantexhaustductisrecommended,with
nomorethanatwoinchgapbetweentheducts,fora1/8inch(3mm)thicksiliconesleeve.IfNuAire
dampervalvesarepresent,seeDrawingBCD05572forinstallation.
14. Ifductdiameterreductionisrequired,itisrecommendedthatthereductionoccursatleast12inches
(305mm)fromthecabinetductconnectionandthatthereductionissmoothandgradualtoreduceair
turbulencethatresultsinnoiseandlossofstaticpressure.
15. ITISNOTRECOMMENDEDTOCONNECTTHECABINETDUCTCONNECTIONDIRECTLYINTOA90DEGREE
BEND.Thecabinet'sexhaustairflowsensorcouldbeaffectedbyairflowturbulencecreatedby90degree
bends.Ifa90degreebendisrequired,itisrecommendedthatthe90degreebendoccuratleast12
inches(305mm)fromtheLFBSCexhaustcollar.
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5.2.8 FinalAssembly
Removetheprotectivecardboardcoveroverthesupplyandexhaustconnectionsontopofthecabinet.The
exteriorsurfaceandviewingglassareeasilycleanedwithanymildhouseholddetergentcleanerusingasoftcloth.
Harshchemicals,solventtypecleanersandabrasivecleanersshouldnotbeused.
DonotattempttocleantheHEPAfiltermedia.Cabinetinteriorwallsorworksurfaceareeasilycleanedwithany
mildhouseholddetergentcleanerusingasoftcloth.Turnthecabinetonandletitoperatefor60minutesbefore
usingitasaLFBSC.
5.3 Exhaust/SupplyAirChecks
NOTE:THEINTERNALSUPPLYBLOWERISINTERLOCKEDWITHTHEEXHAUSTSENSOR,
TOPREVENTOPERATIONUNLESSADEQUATEEXHAUSTFLOWISPRESENT.
5.3.1 ExhaustVolume/InflowVelocity
Theexhaustvolumeandcorrespondinginflowvelocityisdisplayedonthefrontpanel.Presetlowerandupper
alarmlimitsarefactorysetbutcanbefieldverifiedatanytime.Toinsurethatadequateexhaustisavailablefora
dirtyexhaustHEPAfilter,condition,thenominalexhaustreadingsshouldbeattainablewiththebutterflyvalveor
dampersetat60percentopen,withallotherdampersinthesystem(duct)open.
5.3.2 SupplyVelocity
ThesupplyvolumeiscontrolledbytheBSCCairflowcontrolsystem.TheBSCCsystemusesadualthermistor
airflowsensorinthedownflowairstreamtomonitorandcontrolairflowtosetpoint.Thecontrolsystem
automaticallycompensatesforfilterloading,voltagevariancesandotherenvironmentaleffects.However,
excessivevariancescausedbyremotebutterflyvalvesordampers,insufficientsupplyairanddirtyprefiltersmay
causeanairflowalarmconditiontooccur.
NOTE:THESUPPLYAIR,IFINTEGRALLYDESIGNED,MUSTBEINTERLOCKEDWITH
THEINTERNALFANRELAYTOASSUREPROPEROPERATION.
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5.4 CertificationTestingMethodsandEquipment
Afterinstallationandpriortouse,NuAirerecommendsthatthecabinetbecertifiedorcommissionedtofactorystandards.
Asapartofcertification,thecertifiershouldgothroughthefollowinginitialchecklisttoassureallaspectsoftheLFBSC
installationarecompleteandreadyforcertification.
Reviewproductinstallation
Exhaustconnection
Dampervalveinstalledcorrectlywithlabeltowardfront
LFBSCbasestandlevel
Verifyairflowsensorshroudisinplace
Downflow
Verifyconfigurationtypeselectionforspecificmodel*(seesection7.5.2)
Verifysetpointsandalarmlimitsforspecificmodel*(seesection7.5.2)
PerformBCScertification
Ataminimum,thefollowingtestsshouldbeperformed:
HEPAfilterleaktest
Downflowvelocitytest
Inflowvelocitytest
Airflowsmokepatterns
Siteinstallationassessmenttests
PerformSiteAssessmentTests
TheNU430requiresverificationofthesupplyfaninterlockandbackuppressureswitchoperationutilizing
independentexhaustvolumemeasurementinstrument(DIM).PerNSF/ANSI49,a20%lossofexhaust
volumemustproduceanairflowalarmwithin15seconds.
Thetestingmethodsandequipmentrequiredarespecifiedonthefactoryinspectionreportincludedwiththismanual.
(Seeinsertinbackcover)
NOTE: ITISRECOMMENDEDTHATTHESETESTSBEPERFORMEDBYAQUALIFIEDTECHNICIANWHOIS
FAMILIARWITHTHEMETHODSANDPROCEDURESFORCERTIFYINGBIOLOGICALSAFETYCABINETS
(SEEINSERT)
NOTE: AFTERTHEINITIALCERTIFICATION,NUAIRERECOMMENDSTHATTHECABINETBERECERTIFIED(ATA
MINIMUM)ONANANNUALBASISANDAFTEREVERYFILTERCHANGEORMAINTENANCEACTIONOR
ANYTIMETHEOPERATORFEELSITISNECESSARY.
NotethattheLABGARDEScabinets,filters,andsealsprovidepremiumperformance.QualityControlinbothdesignand
manufacturingassuresuperiorreliability.However,protectiontobothproductandoperatorissovitalthatcertificationto
theperformancerequirementsshouldbeaccomplishedasstatedtoensurebiologicalsafetyestablishedbythefactory
standards.
*Ifthespecificmodelisaspecialproductwithnonstandardsetpointsandalarmlimits,thenewvalueswill
belocatedonthefactoryInspectionReport.
OM0228 Page19of99
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LabgardESEnergySaverClassII,TypeB2LaminarFlowBiologicalSafetyCabinet
ModelsNU430400/600
Catalog Number
CatalogNumber NU430400 NU430600
Nominal4foot(1.2m) Nominal6foot(1.8m)
PerformanceSpecifications
1.PersonalProtection NSF/ANSI49 NSF/ANSI49
2.ProductProtection
NSFStd.No.49Class ClassII,TypeB2 ClassII,TypeB2
StyleofCabinet BenchTop/Consolew/BaseStand/ BenchTop/Consolew/BaseStand/
StorageCabinet StorageCabinet
CabinetConstruction AllWeldedStainlessSteel16GA, AllWeldedStainlessSteel16GA,
Type304PressureTightDesign Type304PressureTightDesign
DiffuserforAirSupply(Metal) NonFlammable NonFlammable
HEPAFilterSealType:
SupplyFilter99.99%Eff.on0.3Microns HEPEXSeal HEPEXSeal
ExhaustFilter99.99%Eff.on0.3Microns Neoprene,Springloaded Neoprene,Springloaded
FumigationperNIH/NSFProcedure Yes Yes
StandardServices:
ServiceCoupling(3/8inchNPT) None None
GasValve/ServiceCoupling(3/8inchNPT) Two,RightSidewall Two,RightSidewall
DuplexOutlet One,LeftFrontFaring One,LeftFrontFaring
OptionalServices:GasCocks3/8"NPT Upto3ea.Sidewall Upto3ea.Sidewall
**RemoteControlledValves Upto3ea.Sidewall Upto3ea.Sidewall
UltravioletLight One,Backwall One,Backwall
Standard/CupSinks LeftorRightWorkSurface LeftorRightWorkSurface
CabinetSizeInches(mm):
Height(FullyAssembled) 61(1549) 61(1549)
Height(MinimumforTransport) 61(1549) 61(1549)
Width 535/8(1362) 775/8(1972)
Depth(withControlCenter) 327/8(835) 327/8(835)
WorkAccessOpeningInches(mm):
StandardOpeningHeight 8(203) 8(203)
StandardInflowVelocity 105FPM(.53m/s) 105FPM(.53m/s)
WorkZoneInches(mm):Height 251/2(648) 251/2(648)
Width 463/8(1178) 703/8(1788)
Depth 231/2(597)
ViewingWindowInches(mm): 1.0(25mm)Closed 1.0(25mm)Closed
StandardisTemperedSlidingGlass 181/2(470)Open 181/2(470)Open
HingedTemperedGlass(optional) 8(203)AccessOpening 8(203)AccessOpening
CertificationExhaustValueCFM/CMH 754/1281 1100/1867
ConcurrentBalanceValueCFM/CMH+ 785/1334 1250/2124
PlantDuctStaticPressureEng./Metric 1.7w.g./43mmw.g. 1.8w.g./46mmw.g.
HeatRejected,BTU,PerHour 474 584
Electrical:115V U.L./ULC,USListed U.L./ULC,USListed
Volts,AC(Hz) 115,60 115,60
++Amps:Blower/Lights 2.1 2.6
Amps:Outlet 3 3
RatedAmps: 10 10
12ft.PowerCord(one) 14GA3Wire,15A 14GA3Wire,15A
CratedShippingWeight: 570lbs./259kg. 760lbs./345kg.
NetWeight 520lbs./236kg. 710lbs./322kg.
**Remotecontrolledvalvehandlesprojectthroughfrontfairing.Decorativesidepanelsareavailabletocoverplumbing.
+ConcurrentBalanceValueshallbeusedfordesignandbalanceexhaust/supplyHVACrequirements.
++Basedoncabinetwithnewfiltersrunningat115VAC.
OM0228 Page20of99
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6.0 OperatingtheNU430
6.1 BiologicalSafetyCabinetControl
6.1.1 Overview
TheBiologicalSafetyCabinetControl(BSCC)systemisdesignedtoservicethecontrolrequirementsoftheNU430
BiologicalSafetyCabinet.Thecontrolsystemisaselfcontainedmicroprocessordrivenmodulethatwillperform
thefollowingfunctions:
EasyuserinterfaceviaTOUCHLINKLCD
ControlblowerDCECMMotorviasolidstateDCMotorController
Monitor,displayandcontroldownflow,viadigitaldualthermistorairflowsensor
Monitor,displayandoptionallycontrolexhaustflow(inflow)viadigitaldifferentialvelocitypressure
flowgrid
Alarmsetpoints;high/lowforerrorconditions(downflowandexhaustflow)
Date/Clockdisplayandtimerfunction
Controllightsviasolidstateswitch
Controloutletsviasolidstateswitch
Completediagnosticfunctions
TheNU430BSCCsystemoffersthelatestdigitalmicroprocessordesigntechnologyforimprovedcabinet
performanceandsafety.Thecontrolsystemusesadigitaldualthermistorairflowsensorinthedownflowstream
tomonitorandcontrolairflowtosetpoints.Thecontrolsystemautomaticallycompensatesforfilterloading,
voltagevariancesandotherenvironmentaleffects.Adigitaldifferentialvelocitypressureglowgridintheexhaust
airstreammonitorsforexhaustvolumeandsubsequentinflowvelocity.Downflowvelocity,exhaustvolumeand
inflowvelocityaredisplayedontheTOUCHLINKLCDscreen.Thecontrolsystemalsomonitorstheslidingwindow
positionwithamicroswitchforbothwindowheightandwindowclosedpositions.
Thecontrolsystemthroughtheuseofthefrontpanelcontrolstheon/offfunctionofthefluorescentand
ultravioletlights(optional),outletsandDCECMmotor/blower.Thecontrolsystemalsoallowscontactclosure
outputsforinteractionwithHVACsystemstooptimizeenvironmentalperformance.
UserinterfacetotheBSCCsystemisaccomplishedviatheTOUCHLINKLCD.BasicuseoftheLFBSCisaccomplished
viatheiconslocatedalongthetopofthescreenasshownbelow.Touchanicontoturnon/offfunctionsas
indicated.Eachiconwillilluminatewithcolortoindicatewhenthefunctionisturnedon.Themenuiconwill
alwayspromptamenuscreentodisplay.Selectingamenuitemwillcontinuethepromptsuntilthedesired
parameterisachieved.Toreturntothemainmenu,presstheMENUiconrepeatedlytoreverseoutofthe
parametermenus.
MENU
UV
FL
OM0228 Page21of99
September/2014
6.1.2 StandbyMode
WhentheLFBSCisnotinuse,theTOUCHLINKLCDscreenwilldisplayalargeNuAirelogo,theiconsalongthetop
andthetimeanddateatthebottomrightasshownbelow.Anyofthefunctionicons,excepttheblower,that
initiatesRunMode,maybeturnedonandoffinstandbymode.Thetimerandmenuiconsmayalsobeaccessed
foradditionalusermenus.TheTOUCHLINKLCDdoeshaveascreensaverfunctionbuiltinforextendedLCDlife.
Thedefaultscreensavertimeis60minutes.Thismeansafter60minuteswhentheblowerisnoton,the
TOUCHLINKLCDwillgodark.TobringbacktheTOUCHLINKLCD,justtouchthescreenandthescreensaverwill
reset.Tochangethescreensavertime,accessSCREENSETUPthroughthemenuicon.
MENU
UV
FL
xx:xx AM xx/xx/xx
6.1.3 RunMode
AnytimetheblowericonisselectedtheRunModescreenwillappear.TheRunModescreenwilldisplayaLFBSC
profileandinitiateanddisplaythecountdownofa150secondwarmupperiod.Duringthewarmupperiodthe
asepticcleaningprocessmaybegin.Iftheslidingwindowisraisedanaudibleandvisualalarmwilloccur,butmay
besilencedbypressingthealarmsilenceiconthatappears.
Airflowreadingswillnotbedisplayedduringwarmupperiod.
UV
MENU
FL
WARM-UP - XXX SECS REMAINING
0.0 fpm
0.0 fpm
xx:xx AM xx/xx/xx
Oncethewarmupperiodiscomplete,airflowreadingsandallsystemfunctionswilloperateandbedisplayed.
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6.1.4 Standby/RunModeAlarms
Ifpresent,standby/runmodealarmswillbebothvisualandaudible,theRedLEDovalundertheTOUCHLINKLCD
displaywillturnon,andtheTOUCHLINKLCDscreenwillalsodisplayadescriptionofthealarminplaceofthe
NuAireLogoalongwithalarmsilenceicon.Dependinguponthealarmtype,theLFBSCprofilewillalsoindicatein
redthealarmpresent.Audiblealarmscanbesilencedorwillproduceanalarmtonefor30seconds,thenintoa
ringbackcycleofonceevery10seconds.Pressingthealarmsilenceiconwillsilencetheaudiblealarmfor15
minutes,thenintoaringbackcycleatofonceevery10seconds.
MENU
UV
FL
ACTIVE ALARMS!
WINDOW HIGH!
0.0 fpm
0.0 fpm
xx:xx AM xx/xx/xx
AlarmTypes
WindowHighwindowisraisedaboveitsnominalheight
DownflowHighLimitdownflowisabovethehighalarmsetpoint
DownflowLowLimitdownflowisbelowthelowalarmsetpoint
InflowHighLimitinflowisabovethehighalarmsetpoint
InflowLowLimitinflowisbelowthelowalarmsetpoint
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September/2014
6.1.5 TimerIcons
Thetimericon,whenpressedwillprovidealistoftimefunctionsavailableforuse.
Belowisadescriptionofeachtimerfunction.
MENU
UV
FL
UP
LABORATORY TIMER
PURGE TIMER DOWN
OUTLET TIMER
ENTER
UV LIGHT TIMER
AUTO RUN TIMER
xx:xx AM xx/xx/xx
TimerFunctions
LaboratoryTimerAgeneralpurposetimerthatwhensettoavalue,willcountdownandalarmupontimer
expiration.
UV
MENU
FL
LABORATORY TIMER UP
CURRENT SETTING = 0.00 MINS. SECS.
TIME REMAINING = 0.00 MINS. SECS. DOWN
THE LABORATORY TIMER IS A GENERAL
PURPOSE TIMER THAT WHEN SET TO A VALUE, START
WILL COUNTDOWN AND AUDIBLE ALARM
UPON TIMER EXPIRATION. PRESS UP AND
DOWN TO CHANGE THE SETTING. PRESS
START TO INITIATE THE TIMER.
xx:xx AM xx/xx/xx
PurgeTimerThistimercontrolshowlongtheblowerwillruntopurgethecabinetaftertheblowericonhas
beenpressedtoturnofftheblower.
MENU
UV
FL
PURGE TIMER UP
CORRECT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE
BLOWER WILL RUN TO PURGE THE
CABINET AFTER THE BLOWER ICON HAS SAVE
BEEN PRESSED TO TURN OFF THE BLOWER.
PRESS UP AND DOWN TO CHANGE THE
SETTING. PRESS SAVE TO SAVE NEW
SETTING.
xx:xx AM xx/xx/xx
OM0228 Page24of99
September/2014
OutletTimerThistimercontrolshowlongtheoutletremainsonaftertheoutleticonhasbeenpressedto
turnontheoutlet.Iftimeriszero,theoutletwillstayonuntilturnedoff.
MENU
UV
FL
UP
OUTLET TIMER
CURRENT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE
OUTLET REMAINS POWERED AFTER IT
TURNS ON. THE OUTLET WILL SAVE
AUTOMATICALLY TURN OFF WHEN THE
TIMER EXPIRES. PRESS UP AND DOWN TO
CHANGE THE SETTING. PRESS SAVE TO
SAVE NEW SETTINGS. xx:xx AM xx/xx/xx
UVLightTimerThistimercontrolshowlongtheUVlightwillremainonaftertheUVlighticonhasbeen
pressedtoturnontheUVlight.Iftimeriszero,UVlightwillstayonuntilturnedoff.
MENU
UV
FL
UP
UV LIGHT TIMER
CURRENT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE UV
LIGHT REMAINS ON AFTER IT TURNS ON. THE
UV LIGHT WILL AUTOMATICALLY TURN OFF SAVE
WHEN THE TIMER EXPIRES. PRESS UP AND
DOWN TO CHANGE THE SETTING. PRESS
SAVE TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
AutoRunTimerThistimerprovidestheabilitytoprogramonadailybasisthestartandstoptimeofthe
cabinet.Tostartandstopthecabinetsmenusthatboththeblowerandfluorescentlights
willautomaticallyturnonandofftogetheronaprogrammedschedule.Onceintotheauto
timermenu,selectthedesireddayfortheautotimertofunction.Ifmultipledaysare
desired,eachdaywillberequiredtobesetindividually.
MENU
UV
FL
MONDAY
UP
TUESDAY
WEDNESDAY
DOWN
THURSDAY
FRIDAY
ENTER
SATURDAY
SUNDAY
SETTING AUTO RUN DAYS. SELECT DAY YOU WANT TO SET.
xx:xx AM xx/xx/xx
OM0228 Page25of99
September/2014
Onceintotheselectedday,pressUPorDOWNtoentertheon/offtimes.Usethe>>and<<toselecthoursor
minutes.PressSWITCHtotogglebetweenautotimeonandautotimeoff.PressSAVEaftereachtimeentry.
PressDIStodisableautotimerforthedaybeingreviewed.Repeatautotimerfunctionforeachdayasdesired.
MENU
UV
FL
SETTING MONDAY AUTO TIME ON UP
<<
>> DOWN
SWITCH SAVE
CALIBRATION/SERVICE UP
TIME/DATE
DOWN
PASSWORD
SCREEN SET-UP
FILTER STATUS
xx:xx AM xx/xx/xx
OM0228 Page26of99
September/2014
MenuItems
Calibration/ServiceApasswordprotectedareausedbycertificationorservicepersonneltosetupand
calibratethecabinetforcertificationorcommissioning.
Time/DateThismenuitemprovidestheabilitytosetthetimeanddatedisplayedontheTOUCHLINK
LCDscreen.Timedisplayedisrealtimeandwillnotautomaticallyadjustfordaylightsavingtime.
MENU
UV
FL
UP
DOWN
SET TIME
SET DATE
ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
SETTING CLOCK TIME
<< UP
SWITCH
MENU
UV
FL
SET CURRENT
DATE
UP
<<
DOWN
MM / DD / YYYY
>>
USE >> AND << TO SELECT MONTH, DAY OR YEAR. SAVE
USE UP AND DOWN TO CHANGE VALUE.
PRESS SAVE TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
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September/2014
PasswordThismenuitemprovidestheabilitytorestrictaccesstoturnthebloweronoroffbytheuseofa
password.Toturnonthepassword,pressENABLE/DISABLEPASSWORDandthefollowingscreenwillappear.
ThenturnontheoptionandpressSAVE.Thedefaultpasswordis1234oryoumayusethesetpassword
menutochangethepasswordasdesired.
MENU
UV FL
UP
SET PASSWORD
DOWN
ENABLE/DISABLE PASSWORD
xx:xx AM xx/xx/xx
Enable/DisablePasswordMenu
MENU
UV
FL
BLOWER LOCKOUT ON
OPTION IS OFF
OFF
WHEN BLOWER LOCKOUT IS ON, A
PASSWORD IS REQUIRED TO TURN THE
BLOWER ON OR OFF. WHEN BLOWER SAVE
SetPasswordMenu
EXIT
MENU
0 5
1 6
ENTER OLD PASSWORD
2 7 BACK
XXXX
3 8
ENTER
4 9
xx:xx AM xx/xx/xx
OM0228 Page28of99
September/2014
DefaultPassword
MENU
UV FL
UV
FL
UP
SET BACKGROUND COLORS
DOWN
SCREEN SAVER
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
xx:xx xx/xx/xx
AM
MENU
UV
FL
WHITE
GREEN
BLUE
GRAY
xx:xx AM xx/xx/xx
OM0228 Page29of99
September/2014
MENU
UV
FL
WHITE
GREEN
BLUE
GRAY
xx:xx AM xx/xx/xx
MENU
UV
FL
TOUCH DISPLAY TONE IS ON ON
WHEN THE TOUCH SCREEN TONE IS ON, AN
AUDIBLE TONE WILL SOUND TO INDICATE A OFF
BUTTON PRESS. WHEN OFF IT WILL NOT
SOUND.
USE ON AND OFF TO CHANGE SETTING. SAVE
PRESS ENTER TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
MENU
UV
FL
SCREEN SAVER
OPTION SETTING = 60 ON
FilterStatusThismenuitemprovidesthestatusofavailablefilterlife.
MENU
FILTER LIFE AVAILABLE XX%
OM0228 Page30of99
September/2014
6.1.7 NightSetback(Optional)
Theoptionalnightsetbackfeatureisusedtoreducetheexhaustairvolumeduringnonusageperiodsresultingin
conditionedairenergysavings.Forthenightsetbacktooperate,acontrolvalvemustbeinstalled(i.e.NU951
012motorizedairtightbutterflyvalve)toprovidethemeansforreductionofexhaustairflow.
Thenightsetbackisinitiatedbyeitherclosingthecontactsonthemaincontrolboardorenablingthenight
setbackicononthedisplay.Ifbothareused,thecontactsonthemaincontrolboardhavepriorityoverthedisplay
icon.Oncethecontactisclosedoriconispressedtheinternalblowerandfluorescentlightswillbeturnedoffand
remaininoperable.Theexhaustvalvewillbeclosedtoapercentageoftheoriginalsetpointtypicallytomaintaina
minimumof100fpm(.51m/s)andthedisplaywillindicatenightsetbackactive.Theslidingwindowcanbeclosed
andtheUVlightturnedonifinstalled.
NOTE:IfnightsetbackexhaustairflowisreducedbytheBuildingAutomationSystem(BAS)andnotbytheNuAire
ModelNU951012valve,itwouldstillberecommendedtousethecontactsonthemaincontrolboardtoinitiate
thenightsetbackoptiontodisplaynightsetbackactive,inhibitexhaustalarms,fluorescentlightandinternal
blower.
6.1.8 RemoteOverride(Optional)
Theoptionalremoteoverridefeatureisusedtoremotelycontroltheoperationifthecabinet.Atypicalapplication
wouldbeinaBioSafetyLevelthreefacilitythathadaroomexhaustsystemfailure.
Thefailuremodecouldsignaltheremoteoverridecontactstocloseandnotallowanyusageofthecabinet.Once
theremoteoverridecontactsareclosed,theinternalblowerandfluorescentlightswillbeturnedoffandremain
inoperable.Ifanexhaustmotorizedairtightbutterflyvalve(NU951012)ispresentthevalvewillclosetosealor
optionallyfullyopentheexhaustsystem.
ThedisplaywillindicateRemoteOverrideActive.Oncetheremoteoverridecontactsarebroken,normal
operationwillresume.
OM0228 Page31of99
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6.2 OperatingGuidelines
TheintenthereinistopresentgeneraloperationalguidelinesthatwillaidintheuseoftheLaminarFlowBiologicalSafety
Cabinet(LFBSC)tocontrolairbornecontaminantsoflowtomoderateriskasstatedinTechnicalReportNo.FPS
56500000001preparedbyDowChemicalU.S.A.fortheNationalCancerInstitute,May1,1972.
ProcedureprotocolsdefinedintermsofthebarrierorcontrolconceptsuniquetoLFBSCmustbedevelopedinorderto
obtainamaximumpotentialforsafetyandprotection.Thepreplanningnecessarytodeveloptheseprotocolsisbasedon
severalfundamentalconsiderations,eachofwhichwillcontributetooptimumbenefitsfromtheequipment:
a. Knowyour"SafeWorkArea"
b. Minimizedisruptionof"aircurtain"
c. Minimizeroomactivity
d. Utilizeunidirectionalairflow
e. Employaseptictechniques
6.2.1 KnowYour"SafeWorkingArea"
TheLFBSCsafeworkingareaisbasicallytheworktrayordepressedarea.Allworkshouldbeperformedonor
abovetheworktray.Theareaonorabovethefrontgrillisanonsafeworkingarea.
NOTE:Itisimportanttomaintainanairgaponbothsidesoftheworktraybeforefasteninginplace.
Theworktrayasbeingpartofthecabinethasbeendesignedtoloadupto100lbs.(45.4kg.)ofwork
materials.Anyadditionalloadingshouldbeevaluatedbyappropriatesafetypersonnel.
6.2.2 MinimizePenetrationof"AirCurtain"
Theminimumnumberofitemsnecessaryshouldbeplacedintothecabinettopreventoverloading,butthework
shouldalsobeplannedtominimizethenumberoftimesanoperator'shandsandarmsmustenterandleavethe
aircurtainattheopenface.Theidealsituationistohaveeverythingneededforthecompleteprocedureplacedin
thehoodbeforestarting,sothatnothingneedpassinoroutthroughtheairbarrieratthefaceuntiltheprocedure
iscompleted.Thisisespeciallyimportantinworkingwithmoderateriskagents.
Unnecessaryrisingofthehandsinsidethecabinetabovetheleveloftheworkopeningshouldbeavoided.This
presentsaninclinedplanefromhandstoelbowsalongwhichthedownflowofairmayrunto,andpossiblyout,the
openface.
NOTE:Whenworkingwithagentsoflowerrisk,itisnotasimportantforallmaterialstobeplacedinthe
cabinetbeforestarting,orfortheproceduretobecompletelyfinishedbeforematerialsareremoved.
Also,thetimeperiodforacabinetmaybecontinuedoveramoreextendedperiodduringwhichentries
andwithdrawalsfromthecabinetmaybemade.
6.2.3 MinimizeRoomActivity
Activityintheroomitselfshouldbeheldtoaminimum.Unnecessaryactivitymaycreatedisruptiveaircurrentsas
wellasinterferewiththeworkoftheoperator.Apersonwalkingpastthefrontofacabinetcancausedraft
velocitiesupto175fpm(.89m/s),whicharesufficienttodisrupttheairbalanceofthelaminarflowcabinet.
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6.2.4 UtilizeUnidirectionalAirflow
Theoperatormustkeeptwoimportantfactsinmind:(1)Theairassuppliedtotheworkareathroughfiltersfrom
thetop,iscontaminantfreeand(2)Airbornecontaminationgeneratedintheworkareaiscontrolledbythe
unidirectionalflowofparallelairstreamsinatoptobottomdirection.
Asolidobjectplacedinalaminarairstreamwilldisrupttheparallelflowandconsequently,thecapabilityof
controllinglateralmovementofairborneparticulates.Aconeofturbulenceextendsbelowtheobjectand
laminarityoftheairstreamisnotregaineduntilapointisreacheddownstream,approximatelyequaltothreeto
sixtimesthediameteroftheobject.Withintheparametersofthiscone,particlesmaybecarriedlaterallyby
multidirectionaleddycurrents.
Transferofviablematerialsandmanipulations,whichmaygenerateaerosols,shouldnotbeperformedabove
sterileoruninoculatedmaterials.Itemsshouldbelocalizedontheworksurfacein"clean"and"dirty"groups.
6.2.5 EmployAsepticTechnique
Theoperatormustnotassumeanattitudeof"letthecabinetdoit"whenperformingprocedureswithinaLFBSC.
Properlybalancedandproperlyusedcabinetswilldoanexcellentjobofcontrollingairbornecontaminationand
containingviableagents,butthecabinetwillnoteliminatecontacttransmissionofcontamination.Normal
laboratorycontaminationcontrolproceduresandbasicaseptictechniquesarenecessarytoobtainmaximum
benefitfromthecabinet.Forexamples,openbottle,tubeorflaskmountsshouldbekeptasparallelaspossibleto
thedownflowtominimizecaptureofchanceparticulates.Thisprecautionismerelyanextensionofgoodaseptic
techniqueaspracticedonopenbenchtops.Thegoodlaboratorypracticesdesignedtominimizecreationand/or
releaseofaerosolstotheenvironmentshouldnotbediscontinued.
Itemsofequipmentindirectcontactwiththeetiologicagentmustremaininthecabinetuntilenclosedoruntil
surfacedecontaminated.Traysofdiscardpipettesmustbecoveredbeforeremovalfromthecabinet(aluminum
foilmaysubstituteforfabricatedcovers).
Ifanaccidentoccurswhichspillsorsplatterssuspensionsofetiologicagentaroundtheworkarea,allsurfacesand
itemsinthecabinetmustbesurfacedecontaminatedbeforebeingremoved.
Applyingaburnerflametoflaskandtubeneckswhenmatingsurfacesofsterileassembliesisaconventional
methodofminimizingchancecontamination.However,theefficiencyofthisoperationisusuallyrelatedtothe
removalofairbornecontaminationoccurringwhiletheitemisuncovered.Ifthemanipulationiscarriedoutinan
environmentfreeofairborneparticulates,thentheneedfortheflamingoperationisessentiallyremoved.
ThisisoneoftheadditionaladvantagesoftheLFBSCuseofthegasburnerisseldomnecessary.
Thegasburnerflameinoneofthesecabinetsnotonlycontributessignificantlytotheheatbuildup;italso
disruptsthelaminarairstreams,whichmustbemaintainedformaximumefficiency.IFTHEPROCEDURE
DEMANDSUSEOFAFLAME,ABUNSENBURNERWITHONDEMANDIGNITIONISRECOMMENDED.DONOTUSE
CONSTANTFLAMEGASBURNERS.Itshouldalsobeonlyusedfromthecenteroftheworksurfacetotheright
rearwhereresultingairturbulencewillhaveaminimaleffect.DONOTUSEGASBURNERONTHELEFTOFTHE
WORKSURFACEDUETOITSINFLUENCEONTHEELECTRONICAIRFLOWCONTROLSYSTEM.Ifcabinetairis
inadvertentlyturnedoff,theflamecoulddamagetheHEPAfilters.
OM0228 Page33of99
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6.3 OperatingSequence
6.3.1 StartUp
Turnoncabinetblowerandlights,checkairintakeandexhaustportalsofthecabinettomakesuretheyare
unobstructed.Theelectronicairflowcontrolsystemwillautomaticallycontrolairflowstospecifiedsetpoints.
NOTE: Somecabinetsareequippedwithultraviolet(UV)lights.Goodprocedureincludesthedecontaminationorwipe
downofcabinetsurfaceswithchemicaldisinfectantbeforeworkcommences.Thispracticeeliminatestheneed
forUVlights,whoseprimaryutilityinthisapplicationisinactivationofsurfacecontaminationsincethefilters
effectivelyremoveallairbornecontaminants.UVlights,therefore,arenotrecommendedintheLFBSC.
Allowblowerstooperateforaminimumof15minutesbeforeasepticmanipulationsarebeguninthecabinet.If
thefilteredairexhaustedfromthecabinetisdischargedintotheroom,asinsomeinstallations,anadditional
advantageisobtainedfrompurification(filtration)oftheroomaircirculatedthroughtheequipment.Becauseof
thischaracteristiccontributingtothequalityofthelaboratoryenvironment,someownersofLFBSC'sleavethemin
operationbeyondthetimeofactualuse.
6.3.2 Wipedown
Theinteriorsurfacesoftheworkspaceshouldnextbedisinfected(seecleaningprocedures)bywipingthem
thoroughlywith70%alcoholorsimilarnoncorrosiveantimicrobialagents.USEOFCHLORINATEDORHALOGEN
MATERIALSINTHECABINETMAYDAMAGESTAINLESSSTEEL.
6.3.3 Materials&Equipment
Theapparatusandmaterialsshouldnextbeplacedintothecabinet.Caremustbeexercisedthatnoitemsare
placedoverthefrontintakegrills.Materialsshouldbearrangedsothat,clean,dirty,(used),andvirusmaterials
arewellseparated.Passageofcontaminatedmaterialsoveruninoculatedculturesorcleanglasswareshouldbe
avoidedandtransferofviablematerialsshouldbeperformedasdeeplyintothecabinet(awayfromopenface)as
possible.
6.3.4 AirPurge
Additionalpurgingoftheworkspacewithoutuseractivityshouldbeallowedfor23minutesaftermaterialsand
apparatuseshavebeenplacedinit.Thiswillridtheareaofall"loose"contaminationthatmayhavebeen
introducedwiththeitems.
6.3.5 PerformWork
Theworkcannowbeperformed.Thetechnicianperformingtheworkisencouragedtowearalongsleevedgown
withknitcuffsandrubbergloves.Thiswillminimizethesheddingofskinfloraintotheworkareaandconcurrently
protectthehandsandarmsfromviableagentcontamination.Ataminimum,thehandsandarmsshouldbe
washedwellwithgermicidalsoapbeforeandafterworkinthecabinet.ForthepreparationofAntineoplastic
drugs,thefollowingproceduressummarizethosecontainedinOSHATechnicalManualTED10.15A,SectionVI,
Chapter2ControllingOccupationalExposuretoHazardousDrugs.Theabovedocumentshouldbethoroughly
studiedandreviewedpriortodrugpreparationinthecabinet.Itmaybefoundatthiswebsite.
http://www.osha.gov/dts/osta/
a. Asterileplasticbackedabsorbentdrapeshouldbeplacedontheworksurfaceduringmixingprocedures.
Thedrapeshouldbeexchangedwheneversignificantspillageoccurs,orattheendofeachproduction
sequence.
b. Vialsshouldbeventedwithafilterneedletoeliminateinternalpressureorvacuum.
c. Beforeopeningampoules,careshouldbetakentoinsurethatnoliquidremainsinthetipoftheampoule.
Asterilegauzespongeshouldbewrappedaroundtheneckoftheampoulewhileopening.
d. Finaldrugmeasurementshouldbeperformedpriortoremovingtheneedlefromthestopperofthevial.
e. Anonsplashcollectionvesselshouldbeavailableinthebiologicalsafetycabinettodiscardexcessdrug
solutions.
OM0228 Page34of99
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6.3.6 TerminalPurging&Wipedown
Followingcompletionofwork,allowthecabinettorunfora23minuteperiodwithoutpersonnelactivitytopurge
thecabinet.Thedecontaminationoftheinteriorsurfacesshouldberepeatedafterremovalofallmaterials,
cultures,apparatuses,etc.Acarefulcheckofgrillsanddiffusergridsshouldbemadeforspilledorsplashed
nutrients,whichmaysupportfungusgrowth,andresultingsporeliberationthatcontaminatestheprotectedwork
environment.
6.3.7 PaperCatch/Prefilter
Apermanentpapercatchisinstalledbehindthereardividerpaneloftheworkzone.Thisareaformsthereturnair
pathtothemotor/blower;andiftheairflowisblocked,itcouldseriouslyaffecttheperformanceofthecabinet.
Therefore,THEPAPERCATCHSHOULDBECHECKEDANDCLEANEDNOLESSTHANAWEEKLYBASIS;DAILYbasisif
proceduresdictatetheuseofpaperproducts.AnypaperremovedmustbeproperlydisposedofasContaminated
HazardousWaste.Theaboveproceduresalsoapplytoallcabinetsconfiguredwithaprefilter.
6.3.8 ShutDown
Turnoffblowersandlights.Donotusecabinetasadepositoryforexcesslabequipmentduringperiodsofnon
operation.IfAntineoplasticagentsarebeingpreparedinthecabinet,itisrecommendedtoletthecabinetrun
24hoursperday.Thislessensthepossibilitythatcontaminantsmayescape.
6.4 Ergonomics
Ergonomics,thestudyoraccommodationofworkpracticesisextremelyimportantforpropercabinetusageanduser
healthandsafety.Anevaluationofnormalworkpracticesshouldbeperformedwitheachuserwhenworkinginacabinet.
Evaluationcriteriashouldbeataminimum:
a. Properuserposture
b. Effectiveworkzonelayoutforworkpractice
c. Visionorsightlines
Foreachoftheaboveevaluationcriterion,severalaidsmaybesuppliedtoaccommodatetheuser.
ErgonomicchairAsixwayarticulatingseatandbackcontrolforpersonalizedadjustmenttoassureproperuser
posture.Besurefeetarerestingonthefloor,chairfootsupportorfootrest.Alsobesurebackisfullysupported
withproperchairadjustments
Forearm/armrestsupportThecabinetisprovidedwithaforearmsupportontheworkaccessopening
Periodicminibreaksduringworkpracticeshouldbetakenrestingforearmtoavoidstressandfatigue
EffectiveworkzonelayoutAlwaysprepareyourworkproceduretominimizereachtoavoidneckandshoulder
stressandfatigue.Rotatingtablesareoptionaltomaximumworkzoneandminimizereach
VisionandsightlineAlwaysprepareyourworkproceduretoeliminateglareandbrightreflectionsonthewindow.
Keepyourwindowcleanandsightlinescleartoyoureffectworkzone
6.5 CleaningProcedures
Cleaningthecabinetisanimportantfunctionintermsofbothcontainmentandsterility.Usethefollowingprocedureto
effectivelycleanorsurfacedisinfectthecabinetworkzonesurfaces.
a. Raisetheslidingwindowtoafullopenposition,ifdesired.
b. PresstheAUDIBLEALARMSILENCEorCLEANINGKEYonthefrontcontrolpaneltosilencetheaudiblealarmduring
thecleaningprocess.
c. Applyappropriatedisinfectingsolutiontocabinetsurfaces.Mostsurfacedisinfectantsrequireaspecificcontact
timedependinguponthemicrobiologicalagentsusedwithinthecabinet.CONSULTAPPROPRIATEDISINFECTANT
DOCUMENTATIONFORPROPERAPPLICATIONANDSAFETYPRECAUTIONS.
CAUTION:DISINFECTANTSTHATUSECHLORIDESANDHALOGENSWILLCAUSEDAMAGETOTHESTAINLESS
STEELSURFACESIFLEFTONFORLONGPERIODSOFTIME.
d. Afterthespecifiedcontacttime,wipeupexcessdisinfectant.IFTHEDISINFECTANTUSEDCONTAINSCHLORIDES
ORHALOGENS,REWIPEALLSURFACESWITH70%ALCOHOLORSIMILARNONCORROSIVEANTIMICROBIAL
AGENTTOPREVENTDAMAGETOSTAINLESSSTEELSURFACES.
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6.6 HazardousDrugDecontaminationProcedures
Thisprocedureisintendedtoprovideguidancefollowingaspillageand/orperiodicmaintenance,testingorrelocationof
thecabinet.AdditionalguidancecanbeprovidedbytheCETAdocumentCAG0052007foundattheCETAwebsite:
www.CETAinternational.org.
6.6.1 Preparation
Priortobeginningdecontaminationactivity,personnelshouldwearproperpersonnelprotectionequipment(PPE)
1
i.e.Tyvek isolationgown,2pairofNitrileglovesandafullfacedHEPAfilteredrespirator.Allprotectivegarments
shouldbecontainedin4milplasticbagsandlabeledfordisposalaschemotherapywasteaftercompletionofthe
procedure.Forthepurposeofthisprocedure,detailedproceduresforcleaningaClassIILFBSCcanbefoundin
the2006ASHPTechnicalAssistanceBulletinASHPGuidelinesonHandlingHazardousDrugs2.
6.6.2 Procedure
a. Makesurethatthecabinetremainsinoperationalmodewithinternalbloweron.
b. Openthehingedorslidingviewscreenandsecureinthefullopenposition.
Withtheviewscreeninthefullopenposition,personnelprotection
! CAUTION iscompromisedandafullfacedHEPAfilteredrespiratormustbeworn.
c. Cleanallreadilyaccessiblesurfacesofthecabinet.
d. RemoveperforatedmetaldiffuserscreenfromtheundersideofthesupplyHEPAfilterandplaceonthe
cabinetworktray.
NOTE: Dependingonthemodel,thediffuserscreenissecuredtothecabinetby#832screwsor1/4"20acorn
nuts,3places.Itispurposelyatightfitandissecuredtothebackwallwithprojectingthreadlessstuds.
e. Cleanbothsidesoftheperforatedmetaldiffuserscreenandremoveitfromthecabinet.
f. Liftthecabinetworktray,cleanbothsidesandremoveitfromthecabinet.
g. Removethefrontperforatedgrill,placeonthecabinetfloorandcleanbothsides.
Removefromcabinet.
h. Cleanworktraysupports.
i. Workingfromtoptobottom,cleanallinsidesurfacesofthecabinet.
TakecarenottowettheHEPAfilter.
Ifliquidhascollectedintheplenumdrain,aspirateitusingIVtubingintoanevacuatedcontainer.
Labeltheevacuatedcontainerfordisposalaschemotherapywaste.
j. Cleantheplenumdrainareaandwipedry.
k. Ifthecabinetrequiresmaintenanceand/orreplacementoftheHEPAfilters,theoperationshouldbe
haltedatthispointtoallowtrainedpersonneltocompletereplacementoftheHEPAand/ormaintenance
actionrequired.
6.6.3 Assembly
a. Replacefront(ifremoved)grill.
b. Replacetheworktrayandcarefullytightenthethumbscrews.
c. ReplaceperforatedmetaldiffuserscreenovertheundersideofthesupplyHEPAfilter.
d. Wipedownallexposedsurfacesoftheworkareawith70%isopropylalcohol.
e. Prepareforasepticoperation.
1
AvailablefromLabSafetySupply,Janesville,WI535471368,orotherlaboratory,industrial,orhospitalsupplydistributors.
2
AmericanSocietyofHospitalPharmacists.2006.ASHPGuidelinesonHandlingHazardousDrugsAm.J.Hosp.Pharm.63:11721193.
OM0228 Page36of99
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7.0 GeneralMaintenance
Allmaintenanceactionsonthisequipmentmustbeperformedbyaqualified
technicianwhoisfamiliarwiththepropermaintenanceproceduresrequiredforthis
! CAUTION equipment.Thisincludesbothcertificationaswellasrepair.
7.1 Decontamination
NomaintenanceshouldbeperformedontheinterioroftheLABGARDEScabinet(areabehindaccesspanels)unlessthe
cabinethasbeenmicrobiologicallydecontaminated,isknowntobiologicallyclean,orknowntobechemicallyinert.
Surfacedisinfectionisperformedasspecifiedinthecleaningprocedures.
HazardousGases!PersonalProtectionEquipmentRequired.
! CAUTION
Adisinfectionusingformaldehydemustbeperformedinaccordancewiththespecificationsof
NSF/ANSI49,AnnexG.
Thisprocedurepresentsconsiderablerisksandmustbeperformedonlybyspeciallytrainedand
authorizedservicepersonnelinaccordancewithapplicablesafetyregulations.
Theformaldehydeisvaporizedwithinthetightlysealedsamplechamber.Thequantityoftheapplied
formaldehydedependsonthevolumeofthesamplechamberinthesafetycabinetthatistobe
disinfected.Theformaldehydeevaporatesimmediatelyafterreachingitsboilingpoint;theminimum
reactiontimeis
6hours.Therefore,theformaldehydeshouldbeneutralizedafterthespecifiedreactiontimeby
vaporizingammoniumbicarbonate.
! CAUTION FlammableHazard!
Paraformaldehydeisflammable.Theautoignitiontemperatureofparaformaldehydeis300C(572F).
! CAUTION ChemicalHazard!
ParaformaldehydeinreactionwithhydrogenchloridewillformBCMEwhichisahazardouschemical.
Whenusingparaformaldehyde,allresiduesofhydrogenchlorideintheworkchamberofthecabinet
mustberemoved.
Ifmicrobiologicaldecontaminationisnecessary,usethefollowingprocedure:
1. Removescrewsateachuppersideofthecontrolcenterandallowthecontrolcentertorotatedown,restingon
thesafetystraps.Removecontrolcenterbydisconnectingsafetystrapsandmovingcontrolcentertotheleftoff
thesliphinges.
2. Removethefrontdecorativepanelviatop/frontfasteners.
3. Removeleftandrightwindowfaringsviafasteners.
4. Removearmrestviafasteners.
5. Placedecontaminationequipmentinsidetheworkarea.Referencedecontaminationprocedure,perNSF/ANSI49,
AnnexG,usingthefollowingcharttocalculatechemicalrequirements.
OM0228 Page37of99
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CabinetSize 400 600
60x28x463/8 60x28x703/8
CabinetDimensions
(1.52x.711x1.18m) (1.52x.711x1.8m)
45.1cu.ft. 68.4cu.ft.
CabinetVolume
(1.28cu.m) (1.94cu.m)
NOTE:
Theoutletsintheworkareaareenergizedaslongasthecabinetispluggedinandswitchedonthefront
panel.Unplugthecabinetbeforedecontaminationequipmentispluggedintotheseoutletsorrunthe
decontaminationpowercordsunderthefrontsealarea.
6. Useducttapeandplastictosealthefrontandexhaustarea.
BESURECABINETISTOTALLYSEALEDTOPREVENTANY
! CAUTION
LABORATORYEXPOSURETODECONTAMINATIONGAS.
7. PerformdecontaminationprocedureperNSF/ANSI49,AnnexG.
Ifthecabinethasbeenusedtopreparehazardousdrugs,(chemotherapy),orothertoxicchemicals,decontaminationofthe
cabinetcannotbeaccomplishedbytheaboveprocedure.(Seesection6.6forguidelines)
PleaseconsultwithNuAire,Inc.aboutanyuniquecontaminationproblems.
Normally,nopreventivemaintenanceisrequiredontheinteriorofthecabinet(i.e.,theareabehindtheaccesspanel
containingtheHEPAfiltersandmotor(blowerassembly).Allrequiredadjustmentsinordertomaintainpropercabinet
airflowsareexternaltothecabinetinterior.Themotorislubricatedforlifeandisthermallyprotectedwithautomatic
reset.
7.2 FluorescentLampBulbReplacement
Thetwo(T8)fluorescentbulbsarecoolwhite,rapidstartandplacedexternaltothecabinettoaidmaintenanceand
minimizeheatbuildupwithinthecabinet.Theliferatingofthebulbis9000hoursbasedonthreehourburningcycles.
Toreplaceabulb,itisnecessarytoremovethelampassembly.
1. SwitchCabinetLightSwitchoff.
2. RemovethescrewsateachuppersideoftheControlCenterandallowtheControlCentertorotatedown,resting
onthesafetystraps.
3. Thebulbisnowdirectlyexposedforreplacement.
4. Thebulbisremovedbydisplacingthebulbtoonesideagainstthecompressiblebulbholderandliftingoutthe
bulb.
5. Reversetheproceduretoreinstallthelampassemblybeingcarefulnottopinchthesafetystraps,cableortubing
duringclosureofthecontrolcenter.
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7.3 HEPAFilter/MotorReplacement
TheHEPAFiltersundernormalusageandbarringanaccident(apuncture),donotneedreplacementuntiltheexhaust
volumecannotbemaintainedortheaccessinflowvelocitycannotbemaintainedat100LFPM(min.).51m/s).Thismay
permittheaveragedownflowvelocitytobeaslowas55LFPM(.28m/s)aslongasnopointfallsbelow20percentofthe
averagedownflowvelocity.
TheHEPAFiltersshouldnotbereplaceduntiltheentirecabinethasbeendecontaminatedorknowntobebiologically
"clean".Constantpressurespringtypeclampsareusedtoholdtheexhaustfiltertightlyinplacetocounteractseal
relaxation,whilethesupplyfilteremploysNuAire'sHEPEXpressureplenum.USEONLYREPLACEMENTFILTERSOFTHE
SAMERATEDFLOWANDSIZEASORIGINALLYINSTALLED,TOINSUREPROPERAIRFLOWBALANCECANBEACHIEVED.
Itisnotalwaysnecessarytoreplaceboththesupplyandexhaustfiltersatthesametime.Infact,itishighlylikelythatthe
exhaustfilterwillneedreplacementfarmoreoftenthanthesupplyfilter,dueto(1)thelargervolumeofairpassing
throughit,(2)itsmuchsmallersize,and(3)thecapabilityoftheexhaustsystem.
7.3.1 SupplyFilterReplacement(seeDrawingBCD05659)
Disconnectelectricalpowerfromthecabinetbeforeattemptinganymaintenanceaction.
Step1: Removescrewsateachuppersideofthecontrolcenterandallowthecontrolcentertorotatedown,
restingonthesafetystraps.Second,removethefrontdecorativepanel,whichisheldintopositionby(3)
knurlednutsonthetopedgeand(6)knurledscrewsonthefront.
Step2: Placeslidingwindowintolowestpositionandremovefrontfilterpanel,whichisheldintopositionby
Phillipspanheadscrews.Oncethescrewsareremoved,removethepanel.
NOTE: Screwsareusedinlieuofacornnuts,andlockwashers.
ThescrewshaveOringsandshouldbereplacedifdamagedorbadlydeformed.
Step3: Removebloweraccesspanel,whichisheldintopositionby1/420acornnuts.Oncetheacornnutsare
removed,removethepanel.
Theinteriorofthecabinetisnowfullyexposedforreplacementofthefilter.
Step4:Toremovethesupplyfilter:
a. Unlatchthethreefilterclamps.(InfrontofthesupplyHEPAfilter)Theclampsprovideveryhigh
tensionandmayrequiremechanicalassistancetounfasten.Keepfingerandhandsclearwhen
releasing!
b. Loosenthreeblackhandknobs(about3turns)inbackofpermanentplenum.
c. Liftthepermanentplenumandholdupwithwirestrap.
d. Carefullyremovethesupplyfilter.
DIRECTEXPOSURESHOULDBEAVOIDED,EVENTHOUGHTHEFILTER
! CAUTION
ISINANUNCONTAMINATEDPLENUM.
Step5: Toinstallthesupplyfilter,simplyreversetheprocedureoutlinedinthestepsabove.
SIZEASORIGINALLYINSTALLED.
NOTE: WHENINSTALLINGNEWFILTERS,USEONLYFILTERSOFTHESAMERATEDFLOWAND
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7.3.2 ExhaustFilterReplacement
Step1: Removeexhaustfilteraccesspanel,whichisheldintopositionby1/420acornnuts.
Oncetheacornnutsareremoved,removethepanel.
Step2: Locatetheexternalsinglepointreleaseboltonthetoprighthandsideofthecabinet.
Usea5/16inch(8mm)wrenchtoreleasetheexhaustfilterrotatingcounterclockwise.
Step3: Carefullyremovetheexhaustfilter.
DispenseofspentHEPAfiltersproperly.
Avoiddirectcontactto"dirtyside"ofthefilters.
! CAUTION
Placeinsealedbagandlabelwastecontainers/cartonsbasedonthetypeof
hazard.FollowallLocal,StateandFederalguidelinesfordisposalofHEPA
filtersolidwaste.
Wheninstallingthenewfilter,applyathinlayerofsiliconegreasetothegasketofthefilterandcarefullyinsert
intoexhaustchamber.TightenHEPAsealframe(clockwise)untilthegasketisvisuallydepressedby1/8inch
(3mm).TheprocedureforreplacingtheexhaustfilterwiththeBagin/BagoutoptionisshownonDrawing
BCD05660.
7.3.3 Motor/BlowerAssemblyRemoval
a. Itisrecommendedthatthemotor/blowertoberemovedasasingleunit.Toremove,disconnect
electricalconnectionstothemotor,removetheHEPEXpressureplenumandunboltthemotor/blower
assemblyfromtheroofofthecabinet(4places).Alwaysinspecttherubberisolationmotormountsand
replacethosethatarecrackedorvisiblyshowstress.
b. Replacethemotorexactlyasoriginallyinstalledintheblowerhousing,payingparticularattentiontothe
correctelectricalconnections(seeElectricalSchematic).
c. Reinstallthenewmotor/blowerassembly.
7.4 SlidingWindowReplacement&Adjustment
Theslidingwindowreplacementisaccomplishedbyremovingthefrontdecorativepanel,controlcenter,andwindowglide
assemblies.Theslidingwindowadjustmentmayberequiredduetoeverydayuseoverthelifeofthecabinet.Theleft
windowglideisstationarysinceitcontainsthemicroswitchesthatmonitorwindowheight.Therightwindowglideis
adjustablebyasetscrewandtensionscrewmethod(seeDrawingBCD11818).Whenadjustingtheslidingwindow,be
suretoverifypropermicroswitchoperation.Iftheslidingwindowistooloose,thewindowwillnotproperlyactivatethe
microswitches,thuscausingpotentialoperationalmalfunctionstooccur.
OM0228 Page40of99
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OM0228 Page41of99
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7.5 AirflowControlSystemSetupandCalibration
Failuretocalibrateairflowtothespecifiedrequirementsmay
resultinunsafeconditionsofperformance(i.e.productand/or
! CAUTION
personnelprotection,noiseandvibration)
7.5.1 General
TheoperationoftheNU430cabinetrequiresthatthesetupandcalibrationproceduresbeperformedinorderto
certifyorcommissionthecabinetforusage.ThesetupandcalibrationproceduresperformedONLYBYTHE
CABINETCERTIFIERensurethatcabinet'ssetpointsareverifiedandthattheairflowmonitorsensorsarecalibrated
toreadthecorrectvalues.PressMENUtoaccessCalibration/Serviceparameter.
MENU
UV
FL
UP
CALIBRATION/SERVICE
TIME/DATE
DOWN
PASSWORD
CABINET DECONTAMINATION
ENTER
SCREEN SET-UP
FILTER STATUS
xx:xx AM xx/xx/xx
EntryintotheCalibration/Servicefunctionsrequiresaservicepasswordforentry.Afterpressingthe
Calibration/Servicemenuitem,apasswordscreenwillappear.Thedefaultpasswordis9876.
Oncetheservicepasswordisentered,theCalibration/Servicemenuwillappear.
MENU
0 5 EXIT
UV
FL
1 6
BACK
2 7
ENTER PASSWORD
XXXX
3 8 ENTER
4 9 xx:xx AM xx/xx/xx
7.5.2 Calibration/ServiceMenu
TheCalibration/Servicemenuprovidesalistofsubmenuitemstoaccomplishallservicetasks.
Forairflowcalibration,onlythefirstthreesubmenuitemsareusedinthecalibrationprocess.
MENU
UV
FL
SETPOINTS/LIMITS
SENSOR SETUP
NIGHT SETBACK DOWN
SERVICE
OPTION SETUP
DIAGNOSTICS ENTER
CABINET RESET
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page42of99
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7.5.2.1 CabinetType
Thecabinettypecanbeverifiedinthecontrolsystemandisfactorysetandshouldntrequirealteration.
Thecabinettypedefaultinformationcontrolsunitofmeasure,setpointsandlimitsbasedonthetypeand
sizeofcabinet.TheOEMdisplayedmenuitemreferencestheLOGOstyledisplayedNuAirebeingthe
default.
Toverify,pressCABINETTYPE.Thecurrenttypeofcabinetwillbedesignated.Againtoverify,pressthe
correctTypeandtheModel/Sizeofthecurrentselectedcabinetmodelwillbedesignated.Onceverified
thecorrecttypeandmodelaredesignated,thenpressBACKtoreturntoCalibration/Servicemenu.
MENU
CURRENTLY UV
B2 NU-430/435-400
FL
UP
TYPE A2 (70% RECIRCULATE/ 30% EXHAUST)
DOWN
TYPE B1 (30% RECIRCULATE/ 70%EXHAUST)
xx:xx AM xx/xx/xx
UP
B2 NU-430/435-300
B2 NU-430/435-400
B2 NU-430/435-500
B2 NU-430/435-600 DOWN
B2 NU-430/435-800
B2 NU-430-400(E)
B2 NU-430-600(E)
B2 NU-430-400(D) SAVE
B2 NU-430-600(D)
MORE
xx:xx AM xx/xx/xx
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IftheLFBSChasaspecialdownflowarea(workzone),exhaustductdiametersizeorspecialworkaccess
openingwindowheight,thesemustbeenteredintothecontrolsystemtoassurethecorrectdisplay
values.PressMENUtoaccesstheseadditionalparameters.SelectandSAVEappropriatesizeofeach.
MENU
PREVIOUS SAVE
xx:xx AM xx/xx/xx
UP
14 INCH EXHAUST DIAMETER
xx:xx AM xx/xx/xx
UP
8 INCH
10 INCH DOWN
12 INCH
SAVE
xx:xx AM xx/xx/xx
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7.5.2.2Setpoints/Limits
Theairflowsetpointsandalarmlimitsmayalsobeverifiedoraltered.Typicallythesedefaultvaluesare
factorysetbasedonthecabinettype,modelandsizeaspreviouslydiscussed.However,theymaybe
alteredinspecialcasesformodifiedcabinets.Thesetpointestablishestheairflowvaluesthataretobe
maintained.Thehighlowlimitsestablishthealarmboundariesfromthenominalsetpoint.Thedefault
valueshavebeenestablishedbasedupontheperformancespecificationsandcabinetcomponent
tolerances.
Toverifyoralteranyoftheairflowsetpointsoralarmlimits,pressthemenusetpoints/limitsmenuitem.
Then,pressanyoftheindividualsetpointsoralarmlimitstoverifyand/orchange.PressUPorDOWNto
changenewvalue.PressSAVEtoenternewvalue.
MENU
UV FL
UP
DOWNFLOW SETPOINT
DOWN
DOWNFLOW LOW LIMIT
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
OLD DOWNFLOW SETPOINT 60 fpm
xx:xx AM xx/xx/xx
DefaultvaluesforNU430400 DefaultvaluesforNU430600
Downflowsetpoint60 Downflowsetpoint60
Downflowhighlimit66 Downflowhighlimit66
Downflowlowlimit54 Downflowlowlimit54
Exhaustsetpoint754 Exhaustsetpoint1100
Exhausthighlimit867 Exhausthighlimit1265
Exhaustlowlimit641 Exhaustlowlimit935
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7.5.3 AirflowCalibration
TheNU430AirflowCalibrationconsistsofadjustmentstobalancetheairflowwithinthecabinetandthe
calibrationoftheairflowmonitorsensors.THISWORKSHOULDBEDONEONLYBYAQUALIFIEDTECHNICIAN
WHOCANMEASURETHEAIRFLOWFROMTHEFILTERSWITHASUITABLEVELOMETER.NuAireprovidesone
adjustmenttobalancetheairflowwithinthecabinet.Thisis:PWMsignaladjustviaDCECMmotorcontrolsystem
Theblowerspeedcontrolsystemadjuststhecabinet'ssupplyvolumeofairflowwhilecustomersuppliedexhaust
systemcontrolstheexhaustvolumeofairflow.SinceithasbeenNuAire'sexperiencethatthefiltersmaynot
"load"evenly,bothadjustmentsarenecessaryforpropercabinetperformance.
Thecabinetisconsideredtobecertifiableifthefollowingairflowmeasurementsarepresent:
a. Downflowaverage: 60LFPM5LFPM(.30m/s.025m/s).
b. Inflowaverage: 105LFPM5LFPM(.53m/s.025m/s)usingtheDirectInflowMeasurement
(DIM)methodoralternate3constrictedinflowvelocitymeasurementmethod.
Thecalibrationoftheairflowmonitorsensorsoccursduringthecabinetairflowbalancingprocedure.
Thecalibrationprocedureconsistsusingthedownflowandinflowaveragesachievedandentryofthosevalues
intothecontrolsystem.
DCECMmotorPWMsignalDCvoltageshouldalsobemonitoredandrecordeduponfinalcalibration.TheDC
voltagemaybemonitoredusinganindependentdigitalvoltmeterintheVdcmode.Thetwotestpointsto
measureDCECMmotorvoltagearelocatedontheDCmotorsignalconnectoronthemaincontrolboard.
MOTORVOLTAGETESTPOINTS
BEFORESTARTINGAIRFLOWCALIBRATIONPROCEDURE
LETTHECABINETRUNFORATLEAST10MINUTES.
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7.5.3.1 ExhaustVolumeCalibrationwithouttheNU951012MotorizedButterflyValve
Step1:Activatepowertothecabinet
Turnonexhaustsystem
Accesssensorsetupmenu
MENU
UV
FL
CALIBRATE DOWNFLOW SENSOR UP
CALIBRATE EXHAUST SENSOR
DOWN
FIND NEW DOWNFLOW SENSOR
ENTER
FIND NEW EXHAUST SENSOR
xx:xx AM xx/xx/xx
PressCALIBRATEEXHAUSTSENSORtoaccessindividualsensorcalibrationscreen.
MENU
UV
FL
DOWNFLOW = XX fpm SETPOINT = XX fpm UP
RAW EXHAUST = XXX cfm DOWN
CALIBRATED EXHAUST = XXX cfm
SAVE
USE UP/DOWN TO CHANGE CALIBRATED
EXHAUST, ENTER TO SAVE NEW VALUE
PRESS DOWNFLOW SETPOINT TO CHANGE IT
PRESS MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Step2: Turnonmotor/blowerandspotcheckafewdownflowpointstoverifydownflowisclosetothe
desiredsetpointof60fpm+5fpm.
Step3: Now,measureinflowvelocityusingtherecommendedprocedurefoundinTable7.0.
Ifnecessary,adjusttheexhaustsystemtoachievethecorrectinflowvelocity.UseTable7.1to
relatedownflowandexhaust/inflowvolumesandcorrespondingaverageairflowvelocities.Itis
alwaysdesirabletoachieveairflowvaluesthataresetascloseaspossibletonominal.Thisis
especiallyapplicabletotheinflowvelocityanditsrelationtoexhaustvolume,sincetheexhaust
sensoronlymonitorstheexhaustairflow.Iftheinflowvelocityiscalibratedattheouteredgeof
therange,agreaterchanceforalarmconditionswouldexistduetoitscloserproximitytothe
alarmlimits.
PressUP/DOWNtochangeexhaustvolumetothevaluejustcalculatedusingtheinflowvolume.
NOTE:Assumedownflowvelocityisatthenominalvalueof60fpmforexhaustvolumecalculation
purposesasitwillbeadjustedduringthedownflowcalibrationprocess.
PressSAVEtoentertheexhaustvolumevalue.
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Step4:Verifytheoperationofthemanualmodepressureswitchatthispoint.Lowertheexhaust
volumeto100CFM(170CMH)lowerthanthestandardexhaustloweralarmlimit.
Turnoffmotor/blower
Blockoffsupplyductconnectionusingcardboard.
Lowerexhaustvolumetothepressureswitchlowtrippointstatedbelow.
Model LowAlarmLimit PressureSwitchLowTripPoint
CFM(CMH) CFM(CMH)
NU430400 641(1089) 541(919)
NU430600 935(1589) 835(1419)
Ifpresent,removecardboardfromsupplyairintakeandturnONtheunitsblowerifitisnotONnow.
Remove1screwfromeachofthetoprightandleftsidesofthecontrolboxandrotatecontrolbox
forwardtoaccessthepressureswitchadjustment.Pressureswitchmustbeadjustedwhilecontrolbox
isopen.Adjustasneeded(theadjustmentdial),untiltheinternalsupplyblower/motorisdeactivated.
Thenraisetheexhaustvolumebackuptothestandardloweralarmlimittoensuretheinternalsupply
blower/motorturnsbackON.Finally,adjusttheexhaustsystembacktothecorrectinflowvelocityand
exhaustvolume.Reattachcontrolboxandtesttheactivation/deactivationofthepressureswitchagain
whenthecontrolboxisbackintheverticalposition.
Step5: Ifpresent,removedirectreadinginstrumentfromwindowaccessopenarea.
7.5.3.2 ExhaustVolumeCalibrationwiththeNU951012MotorizedButterflyValve
Ifdesired,activateexhaustcontrol/autozero.
TheexhaustcontrolautozerooptionallowstheNU951automaticdampertocheckitselfonceevery24hours
fromitsoriginalcalibrationpoint.Duringtheairflowcalibrationprocedure,oncethecertifiercalibratesthe
exhaustvolumethecontrolsystemwillrememberthedamperpositionindegreesatthatpoint.Thenbased
ontheprogrammedtime,theexhaustsystemwillperformthefollowingautozerosequence.First,freezethe
exhaustdisplaytoitssetpoint.Thenmovethedamperbacktoitsoriginalcalibrationpoint.Allowthedamper
tocontrolnormallyfortwominutesthenunfreezethedisplay.Thissequencewillthenbeperformedatthe
programmedtimeonceevery24hours.TheautozerosequencecanbecheckedbypressingTESTtoinitiatea
fourminutetestsequence.
Toactivatetheexhaustcontrol/autozero,performthefollowing:
SelectOptionSetUp.
MENU
UV FL
UP
SETPOINTS/LIMITS
SENSOR SETUP
NIGHT SETBACK DOWN
SERVICE
OPTION SETUP
DIAGNOSTICS ENTER
CABINET RESET
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page48of99
September/2014
SelectExhaustControlAutoZero.
MENU
UV
FL
MENU
UV
FL
ON
SAVE
WHEN THE EXHAUST DAMPER AUTO ZEROING IS ON,
AN AUTOMATIC CHECK OF THE DAMPER TIME
CALIBRATION POINT OCCURS AT SET TIME. WHEN
OFF, NO CHECKS WILL OCCUR. PRESS TIME TO TEST
CHANGE CHECK TIME. PRESS TEST TO START A ZERO
CHECK ROW.
xx:xx AM xx/xx/xx
Step1:Activatepowertothecabinet
Turnonexhaustsystem
Accesssensorsetupmenu
MENU
UV
FL
SETPOINTS/LIMITS ON
SENSOR SETUP
NIGHT SETBACK
SERVICE OFF
OPTION SETUP
DIAGNOSTICS
CABINET RESET ENTER
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page49of99
September/2014
MENU
UV
FL
UP
DAMPER CALIBRATION
OPEN
60% DOWN
CLOSED
Setdamperpositionto60onthedisplay.NuAirerecommendsusingthedamperposition
at60tominimizestaticlossandmaximumcontrollabilityofthesystem.However,THE
DAMPERCANRANGEFROM35TO75FORITSNOMINALSETPOINT.ANYPOSITIONOUTSIDE
THISRANGEWILLSEVERELYLIMITTHECONTROLSYSTEMSABILITYTOACCURATELYCONTROL
THEEXHAUSTVOLUME.
Step2:Turnonmotor/blowerandspotcheckafewdownflowpointstoverifydownflowisclosetothe
desiredsetpointof60fpm+5fpm.
Step3:Now,measureinflowvelocityusingtherecommendedprocedurefoundinTable7.0.If
necessary,adjusttheexhaustsystemand/ordampertoachievethecorrectinflowvelocity.
UseTable7.1torelatedownflowandexhaust/inflowvolumesandcorrespondingaverage
airflowvelocities.Itisalwaysdesirabletoachieveairflowvaluesthataresetascloseaspossible
tonominal.Iftheinflowvelocityiscalibratedattheouteredgeoftherange,agreaterchance
foralarmconditionswouldexistduetoitscloserproximitytothealarmlimits.
Oncedamperpositionand/orexhaustvolumeisset,pressSAVEtoenterthedamperposition.
PressCALEXtoexitdirectlyintoexhaustsensorcalibrationmenu.
PressUP/DOWNtochangeexhaustvolumetothevaluejustcalibratedusingtheinflowvolume.
NOTE:Assumedownflowvelocityisatthenominalvalueof60fpmforexhaustvolumecalculationpurposesasit
willbeadjustedduringthedownflowcalibrationprocess.
PressSAVEtoentertheexhaustvolumevalue.
Step4: Verifytheoperationofthemanualmodepressureswitchatthispoint.Lowertheexhaust
volumeto100CFM(170CMH)lowerthanthestandardexhaustloweralarmlimit.
Turnoffmotor/blower
Blockoffsupplyductconnectionusingcardboard.
Lowerexhaustvolumetothepressureswitchlowtrippointstatedbelow
Model LowAlarmLimit PressureSwitchLowTripPoint
CFM(CMH) CFM(CMH)
NU430400 641(1089) 541(919)
NU430600 935(1589) 835(1419)
OM0228 Page50of99
September/2014
Ifpresent,removecardboardfromsupplyairintakeandturnONtheunitsblowerifitisnotONnow.
Remove1screwfromeachofthetoprightandleftsidesofthecontrolboxandrotatecontrolbox
forwardtoaccessthepressureswitchadjustment.Pressureswitchmustbeadjustedwhilecontrolbox
isopen.Adjustasneeded(theadjustmentdial),untiltheinternalsupplyblower/motorisdeactivated.
Thenraisetheexhaustvolumebackuptothestandardloweralarmlimittoensuretheinternalsupply
blower/motorturnsbackON.Finally,adjusttheexhaustsystembacktothecorrectinflowvelocityand
exhaustvolume.Reattachcontrolboxandtesttheactivation/deactivationofthepressureswitchagain
whenthecontrolboxisbackintheverticalposition.
MENU
UV
FL
UP
DOWNFLOW = XX fpm SETPOINT = XX fpm
DOWN
RAW EXHAUST = XXX cfm
xx:xx AM xx/xx/xx
Step5: Ifpresent,removedirectreadinginstrumentfromwindowaccessopenarea.
7.5.3.3 DownflowCalibration
NOTE:IfalreadyinCalibration/Servicemenu,selectCALIBRATEDOWNFLOWSENSOR.
Step1:AccessCalibration/Servicemenu.
PressSENSORSETUPmenuitemtoaccesssensorcalibrationmenu.
MENU
UV
FL
UP
CALIBRATE DOWNFLOW SENSOR
CALIBRATE EXHAUST SENSOR
DOWN
FIND NEW DOWNFLOW SENSOR
ENTER
FIND NEW EXHAUST SENSOR
xx:xx AM xx/xx/xx
OM0228 Page51of99
September/2014
Step2: PressCALIBRATEDOWNFLOWSENSORtoaccessindividualcalibrationscreen.PressBLOWER
switchtoon.Allowblowertorunforoneminuteoruntildownflowreadingsaresteady.
MENU
UV
FL
MOTOR RPM = XXXX
UP
BLOWER DUTY CYCLE = XXX
RAW DOWNFLOW = XX fpm DOWN
CALIBRATED
DOWNFLOW = XX fpm
SAVE
USE UP/DOWN TO CHANGE CALIBRATED
DOWNFLOW, PRESS ENTER TO SAVE NEW VALUE
PRESS BLOWER DUTY CYCLE TO CHANGE IT
PRESS MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Step3: Placeavelometerinthecabinetworkzoneonthehorizontalplane4inches(102mm)abovethe
bottomedgeofthewindow.Spotcheckseveralpointsontherecommendeddownflowvelocity
testgridfoundintable7.0.
Step4: PressBLOWERDUTYCYCLEtoadjustblowerspeed.
Theobjectiveofthisspotcheckistoobtainthedesireddownflowaveragevelocityascloseas
possibletothestatedgoalof60LFPM(.30m/s).
MENU
UV
FL
UP
MOTOR RPM = XXXX
BLOWER DUTY CYCLE = XXX DOWN
PRESS UP/DOWN TO CHANGE BLOWER DUTY CYCLE. SAVE
PRESS MENU TO EXIT WITHOUT CHANGE.
xx:xx AM xx/xx/xx
DON'TSPENDMORETHAN5MINUTESSPOTCHECKING.
FINALADJUSTMENTSWILLBEMADEINTHEFOLLOWINGSTEPS.
PressSAVEtoenterthecurrentblowerspeed.
Step5: Now,measuretheaveragedownflowvelocityovertheentireworkzoneusingtherecommended
downflowvelocitytestgrid(seeTable7.0).
OM0228 Page52of99
September/2014
Step6: PressUPorDOWNarrowstochangethecalibrateddownflowvaluetotheaveragedownflow
velocityjustfound.
PressSAVEtoenterthenewcalibrateddownflowvalue.
Nowthedownflowmonitorsensorhasbeencalibratedtotheactualmeasuredaverage
downflowvelocity.Thecabinetwillnowcontroltothedownflowsetpoint.
NOTE: Supplyvolumemaynowbemeasuredandcalculated.Usingthegridscaleof4inchesby
4inches(102mmby102mm),2inches(51mm)awayfromallperimeterwallsand6inches
(152mm)fromthediffuser,measuresupplyvelocityinthesamemannerasthedownflow
velocity.Oncetheaveragesupplyvelocityisobtained,calculatethesupplyvolumeand
correspondinginflowvolumeandvelocityusingtheTables7.0and7.1.
Note: SinceNuAirecabinetsuseafullsupplydiffuser,resultinginuniformairflowvelocities,
NuAirehasfoundthattheaveragesupplyvelocityandtheaveragedownflowvelocityare
typicallyfoundtobethesamevalueorcanberelatedwithasmallcorrectionfactor.
Usingtheaveragedownflowvelocityoracorrectedaveragedownflowvelocity(see
individualmodelnumbersbelow)tocalculatethesupplyvolumeisconsidered
acceptabletocertifyaNuAirecabinet.Usethesupplyvolumeminusthetotalvolumeto
calculateinflowvolumeandaverageinflowvelocity.
ModelNumber SupplyVolumeCorrectionFactor
NU430400 Averagedownflowvelocityplus(4fpm)
NU430600 Averagedownflowvelocity
Now,thecalibrationprocedureiscomplete.Ifdesired,aspotcheckinthedownflow
velocitymaybeperformediffeltnecessary.
PressMENUtoexit.
7.5.3.4 NightSetbackCalibration(Optional)
Thenightsetbackcalibrationisperformedwithinthecalibrationmodeaftertheinflowanddownflow
calibrationprocedureiscomplete.
NOTE: IfnightsetbackexhaustairflowisreducedbytheBuildingAutomationSystem(BAS)andnotby
theNuAiremodelNU951012valve,thiscalibrationprocedureisnotnecessary.However,it
wouldstillberecommendedtousethecontactsonthemaincontrolboardtoinitiatethenight
setbackoptiontodisplaynightsetbackactive,inhibitexhaustalarms,fluorescentlight,and
internalblower.
SelectNightSetback
MENU
UV
FL
SETPOINTS/LIMITS UP
SENSOR SETUP
NIGHT
SETBACK
DOWN
SERVICE
OPTION SETUP
DIAGNOSTICS
CABINET RESET ENTER
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page53of99
September/2014
Step1:Determinehownightsetbackisinitiated.Ifusingcontactsonmaincontrolboard,proceedto
step2.Ifusingthedisplayicon,enablenightsetbackoptionbyperformingthefollowing:
SelectENABLENIGHTSETBACK
MENU
UV
FL
UP
ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
ON
NIGHT SETBACK
OPTION IS OFF
OFF
WHEN THE NIGHT SETBACK OPTION IS ON,
THE NIGHT SETBACK ICON WILL APPEAR ON
THE DISPLAY TO TURN NIGHT SETBACK
SAVE
ON/OFF.
PRESS SAVE TO ENTER SELECTION.
xx:xx AM xx/xx/xx
Step2:SelectCALIBRATENIGHTSETBACK
MENU
UV
FL
UP
ENTER
xx:xx AM xx/xx/xx
OM0228 Page54of99
September/2014
Step3:Atthispoint,thenightsetbackisactiveandthebutterflyvalvewillbegintoclose.Nowmeasure
theinflowvolumeusingaDirectReadingInstrumentofinflowvelocitymeasurementmethod.
Thegoalistoreducetheinflowvolumetothefollowingvalues,whichrepresentsapproximately
100fpm(.51m/s)inflow,ormaybesetatanyothervalueswithinthedamperoperationalrange
of30%to80%.
NU430400 270CFM/459CMH
NU430600 410CFM/697CMH
Toaltertheinflowvolumeinnightsetbackcalibration,pressUPorDOWNarrowtoincreaseor
decreasetheairvolumetothedesirednightsetbackoperatingpercentagevalue.
Pleasenote,inthenightsetbackoperation,theelectronicairflowcontrolsystemwillcausethe
butterflyvalveactuatortohuntmoreoperatingatlowerairflowvolumes.Whentakingthe
inflowvolumemeasurements,averageseveralreadingstoobtainthemostaccurateresults.
MENU
UV
FL
NIGHT SETBACK UP
SETBACK = 80 PERCENT
OM0228 Page55of99
September/2014
7.6 HEPAFilterLeakTest
Inordertocheckfilterandfiltersealintegrity,theHEPAfiltermediaandsealsmustbedirectlyaccessible,bythemeasuring
instrument.Thechallengematerial(i.e.PAO)shouldbesuppliedoverthesupplyinletforthesupplyfilterandintherear
centeroftheworkzoneovertheintakeslotsfortheexhaustfilter.Theupstreamchallengeportforeachfilterislocatedon
topofthecabinet.
7.6.1 SupplyFilter
ThediffuserplateplacedbelowtheHEPAtoprotectthefilterduringnormalusagemayberemovedasfollows:
Thediffuserissecuredtothecabinetshellby#1/420acornnutslocatedimmediatelybehindthefrontviewing
window.Afterremovingthefasteners,dropthefrontofthediffuserplateseveralinchesandpullforwardgently.
Notethatthediffuserispurposelyatightfititisheldtothebackwallofthecabinetinteriorbyalightpushfit
withprojectingstuds.
7.6.2 ExhaustFilter
Theexhaustfilterischeckedusingagrossleakmethod,sincetheexhaustfilterisnoteasilyscannedwhen
connectedtoanexhaustsystem.Itmaybeleaktestedbydrillingaholeintheductatadownstreamlocationthat
willproduceawellmixedaerosolandinsertingthesamplingprobewithrigidextensiontubingthroughhole.
NOTE:
Toavoidthewindowhighalarmduringthefilterintegritycheck,itisdesirabletoenterintothe
Calibration/Servicemenuandturnthebloweron.Toaccomplishthis,performthefollowing:
SelectCalibration/Service
MENU
UV
FL
UP
CALIBRATION/SERVICE
TIME/DATE
PASSWORD DOWN
CABINET DECONTAMINATION
SCREEN SETUP
FILTER STATUS ENTER
xx:xx AM xx/xx/xx
EntryintotheCalibration/Servicefunctionsrequiresaservicepasswordforentry.Afterpressing
Calibration/Servicemenuitem,apasswordscreenwillappear.Thedefaultpasswordis9876.
OncetheservicepasswordisenteredtheCalibration/Servicemenuwillappear.
MENU
0 5
EXIT
1 6
ENTER PASSWORD
2 7 BACK
XXXX
3 8
ENTER
4 9
xx:xx AM xx/xx/xx
OM0228 Page56of99
September/2014
NOTE: Iftheupstreamchallengeportisdeemedcontaminatedandnotaccessible,usebothdownflowand
exhaustvolumefordeterminingchallengeconcentrations.Usefollowingareainformationbelowwith
averagedownflowvelocityandspotcheckexhaustvelocitiesasmeasuredtodeterminevolume(CFM)
(CMH).Useexhaustvolumeasgiven.
ModelSize *SupplyArea(ft2)(m2) ExhaustVolumeCFM(CMH)
400 7.57(.214) 754(1281)
600 11.48(.325) 1100(1869)
*Measured4inches(102mm)abovethebottomedgeofthewindow.
LaskinNozzleConcentrationFormula
#Nozzlesx135CFMx100ug/L Challenge
=
Downflow(CFM)+Exhaust(CFM) Concentration(ug/L)
#Nozzlesx229CMHx100ug/L = Challenge
Downflow(CMH)+Exhaust(CFM) Concentration(ug/L)
7.7 AirflowSmokePatternTest
Theairflowsmokepatterntestisperformedusingasmokesource(i.e.smoketubes)inandaroundthecabinetworkzone
andaccessopeningtodetermineavisualrepresentationofthecabinetscontainmentperformance.Toperformthetest,
thesmokesourceshouldbepassedthroughthefollowingareas:
1. Asmokesourceshallbepassedfromoneendofthecabinettotheother,alongthecenterlineofthework
surface,ataheightof4inches(102mm)abovethetopoftheaccessopening.
2. Asmokesourceshallbepassedfromoneendofthecabinettotheother,1inch(25mm)justinsidetheview
screen,ataheight6inches(152mm)abovethetopoftheaccessopening.
3. Passasmokesourcealongtheedgesoftheentireperimeteroftheworkopeningapproximately1.5inches
(38mm)outsidethecabinet,withparticularattentionpaidtocornersandverticaledges.
4. Passasmokesource2inches(51mm)fromthesidesupinsideofthewindowatthesidechannelseals,andalong
insideofthecabinetalongthetopoftheworkareaorimmediatelybelowthewipergasket.
Thecriteriausedtoevaluatethesmokepatternsisthefollowing:
1. Thesmokeinsidethecabinetshallshowsmoothdownwardflowwithnodeadspotsorreflux.
2. Nosmokeshallescapefrominsidethecabinet.
3. Nosmokerefluxesoutofthecabinetoncedrawnin,nordoessmokebillowovertheworksurfaceorpenetrate
ontoit.
4. Nosmokeshallescapefromthecabinet.
7.8 SiteInstallationAssessmentTests
Thesetestsareperformedtoverifythesashposition,airfloworpressuresetpointwhereanaudibleand/orvisualalarmwill
activatetosignifyunfavorableoperatingconditionswithinthebiologicalsafetycabinetand/ortheremoteexhaustblower.
7.8.1 SashAlarm
Step1:Withsashalarmswitchenabled,raisetheslidingsash1inch(2.5cm)abovethemanufacturers
designatedsashheightfornormaloperation.Verifythattheaudible/visualalarmactivates/sounds.
Step2: Returnthesashtoitsnormaloperatingheight.
7.8.2 AirfloworPressureAlarm(VerifiedDuringExhaustAirflowCalibration)
Step1: Usingtheprimaryorsecondaryinflowtestmethod,lowertheexhaustairflowtoreducethetotal
flowby20%fromthecertifiedtestingvalue.
Step2: Verifythatthealarmactivateswhenthetotalflowhasdroppedtothispoint.
Step3: Adjustalarmsetpointasnecessary.
OM0228 Page57of99
September/2014
7.9 CleanlinessClassificationTestforPharmacyApplication
Ifthiscabinetisgoingtobeusedwithinpharmacy,perUSP7971,thecabinetmustbetestedtoassurecompliancetoISO
146441:1999,CleanroomsandAssociatedControlledEnvironments,Part1:ClassificationofAirCleanliness2.The
cleanlinessclassificationtestisperformedusingaparticlecountertomeasureparticlecountswithinthecabinetworkzone.
Turnoncabinetandletwarmupforseveralminutes.Turnonparticlecounterandflushoutsampletubinglinetoremove
latentparticles.Settheparticlecountertomeasure0.5micronorlargerparticlesattheappropriatemeasuringrate.
OperationalParticleCountTest3
Positiontheparticlecounterisokineticprobeatapoint6inches(152mm)upstreamoftheasepticmanipulationarea(hand
convergencepoint)andmountedsoasnottointerferewiththeoperatorshandmovement.Thepharmacyoperatorwill
simulateIVmanipulationduringtheparticlecounttestusingnonhazardousmaterials.Aminimumofthree(3)1minute
particlecountsshallbesampledandrecordedwhiletheusersimulatesasepticcompoundingmanipulations.
AtRestParticleCountTest
Take5testpointsin1minuteintervalsonagrid,inahorizontalplaneasmeasuredapproximately6inches(152mm)above
theworksurface.Thegridlocationisdesignedastheworkzonecenterpointandeachcornermeasured6inches(152mm)
fromtheinsideperimeter.
Recordthe5particlecountvaluesforeachofthetestpointsoverthe1minutesampletime.Allfinalcountparticle
concentrationsandcalculated95%upperconfidencelimitshallnotexceed3520particlespercubicmeter(ppcm)or(100
particlespercubicfeet(ppcf).
1
USP28NF23:UnitedStatedPharmacopeialConvention,Inc.,12601TwinbrookParkway,Rockville,MD20852,USA,www.usp.org.
2
ISO146441:1999CleanroomsandAssociatedControlledEnvironmentsClassificationofAirCleanliness,InternationalOrganizationforStandardization,
CasePostale56,CH1211Geneve20,Switzerland
3
CAG0022006:CETACompoundingIsolatorTestingGuide,ControlledEnvironmentTestingAssociation,1500SundayDrive,Suite102,Raleigh,NC27607,
USA,www.cetainternational.org
OM0228 Page58of99
September/2014
Table7.0
RecommendedMeasurementMethodsforCabinetDownflow&Inflow
A. DownflowMeasurement
a. Instruments:TSI8355Thermoanemometer
b. Procedure:Supplyfiltereffluxismeasuredonagrid,inahorizontalplanedefinedby4inches(102mm)abovethe
bottomedgeofthewindow.Noreadingshouldbetakencloserthan6inches(152mm)fromtheinsideperimeter.
c. TestDataInches(mm):
6 11.729 17.458 23.187 28.916 34.645 40.375
400
(152) (298) (443) (589) (735) (880) (1026)
6 11.838 17.676 23.514 29.352 35.190 41.028 46.866 52.704 58.542 64.375
600
(152) (301) (449) (597) (746) (894) (1042) (1190) (1339) (1487) (1635)
6
(152)
11.750
(298)
17.5
(44.5)
B. Inflow/ExhaustVolumeMeasurement
a. Instrument:ShortridgeFlowhoodADM870orTSI8355Thermoanemometer.
b. Procedure:Theexhaustairflow(customersupplied)shalldrawairfromthecabinet.Anyoneofanumberofairflow
controllingandmeasuringmeansmaybeusedtoestablishinflow/exhaustvolume.Theinflow/exhaustvolumeis
establishedforthecabinethavingtheworkzonedownflowaveragevelocityatitsnominalvalue.Tomeasuretheinflow
volume,theinternalblowershouldbeturnedon,thus,theinflowvolumebalancedwiththedownflowvolumecanbe
measuredtosetthedesiredaverageinflowvelocity.
Theinflow/exhaustvolumeismeasuredbyusingaDirectInflowMeasurement(DIM)instrument(i.e.Shortridge
Flowhood).TheDIMinstrumentcanbeuseddirectlyonthecabinetwithNOCORRECTIONFACTORSREQUIRED.The
DIMinstrumentshouldbeequippedwithaflowhoodthatisascloseaspossibletothewidthofthecabinet(i.e.NU
430400shoulduse1x4footflowhood).TheDIMinstrumentshouldalsobeducttapedtothecabinetsfrontwork
accessopeningtopreventanysneakairpathsfromoccurring.TheDIMinstrumentwillreadinflowvolume(i.e.CFM).
Usetheareatabletocalculatetheinflowvelocityandreferencedexhaustvolume(CertificationvalueslistedinTable
7.1)basedupontheDIMmeasurement.
AlternateProcedure:
Thealternateproceduretodetermineinflowvelocityusesathermoanemometerinaconstrictedwindowaccessopening
of3inches(76mm)withthedownflowbloweronandthearmrestremoved.Inflowairvelocityismeasuredinthecenterof
theconstrictedopening11/2inches(38mm)abovetheworkaccessopeningonthefollowingspecifiedgrid.Usethearea
correctionfactortabletocalculatetheinflowvelocityandreferenceexhaustvolume.
(CertificationvalueslistedinTable7.1)
NOTE: SinceNuAirecabinetsuseafullsupplydiffuser,resultinginuniformairflowvelocities,NuAirehasfound
thattheaveragesupplyvelocityandtheaveragedownflowvelocityaretypicallyfoundtobethesame
valueorcanberelatedwithasmallcorrectionfactor.Usingtheaveragedownflowvelocityoracorrected
averagedownflowvelocity(seeindividualmodelnumbersbelow)tocalculatethesupplyvolumeis
consideredacceptabletocertifyaNuAirecabinet.
OM0228 Page59of99
September/2014
ModelNumber SupplyVolumeCorrectionFactor
NU430400 Averagedownflowvelocityplus(4fpm)
NU430600 Averagedownflowvelocity
I.E. NU430400 I.E. NU430600
Averagedownflowvelocity=60fpm Averagedownflowvelocity=60fpm
Averagesupplyvolumevelocity=60+4=64fpm Averagesupplyvolumevelocity=60fpm
Supplyvolume=64x7.57=484CFM Supplyvolume60x11.48=689CFM
c. TestDataInches(mm):
1. DIMMeasurement
InflowVolume ft.3/min.(m3/s) AccessOpeningAreaft.2(m2) =InflowVelocityft./min(m/s)
=TotalExhaustft.3/min.(m3/s)
InflowVolumeft.3/min.(m3/s) +SupplyVolumeft.3/min.(m3/s)
Volume
2. Constricted3inch(76mm)highaccessopeningmeasurementInches(mm):
4 8.264 12.528 16.792 21.056 25.320 29.584 33.848 38.112 42.375
400
(102) (210) (318) (426) (535) (643) (751) (860) (968) (1076)
4 8.158 12.316 16.474 20.632 24.790 28.948 33.106 37.264 41.422 45.580 49.738 53.896 58.054 62.212 66.375
600
(102) (207) (313) (418) (524) (630) (735) (841) (946) (1052) (1158) (1263) (1369) (1475) (1580) (1686)
NumberofReadings: AverageVelocityofConstrictedAreaft./min.(mps)
1. AverageVelocityofConstrictedArea fpm(m/s)
2. X ConstrictedAccessArea ft2(m2)
3. = ConstrictedAreaVolume CFM(m3/s)
4. ConstrictedAreaVolume CFM(m3/s)
5. 8"(203mm)AccessWindowArea ft2(m2)
6. = AverageVelocityof8"(203mm)AccessWindowArea fpm(m/s)
7. AverageVelocityof8"(203mm)AccessWindowArea fpm(m/s)
8. X CorrectionFactorfor8"(203mm)Window
9. = AverageInflowVelocity fpm(m/s)
d. AcceptanceCriteria:Accessopeninginflowvelocity=100to110fpm(.51to.56m/s)
Areas/CorrectionFactorsforCalculations
Cab. 3(76mm)ConstrictedWindow 8(203mm)WindowAccess CorrectionFactorfor8 WorkZoneArea
Size AccessAreaft2,(m2) OpeningAreaft2,(m2) (203mm)Window ft2,(m2)
.97 2.58 7.57
400 1.0
(.090) (.239) (.703)
1.47 3.91 11.48
600 1.05
(.137) (.363) (1.066)
OM0228 Page60of99
September/2014
Table7.1
CertificationValues
Thefollowingarerecommendedminimum/maximumcabinetairflowcertificationsetpointsperNSF/ANSI49.NuAire
recommends,however,operationatthestatedaverageflow,foreaseofmaintenanceandannualcertification.
THEFOLLOWINGEXHAUSTFLOWSAREFORAN8INCH(203MM)WORKACCESSOPENING:
NOTE:1:NuAirerecommendsthecabinetbesetupandcertifiedatthestatednominalaverageinflow.
EXAMPLE:
NU430400InflowVolumeMeasurement/Calibration
1) InflowvolumemeasuredwithDIMatcabinetfacewithbloweronanddownflowvelocityapproximatelyat60fpm
(.30m/s),inflowvolume=271(460CMH).
2) Inflowvolume+supplyvolume=exhaustvolume
271cfm(460CMH)+483cfm(821CMH)=754cfm(1281CMH)
3) So,basedontheinflowmeasurementvolumeandaddingthesupplyvolumefromthetableforthenominal
averagevelocity,thecalibratedexhaustvalueenteredshouldbe754cfm(1281CMH).
OM0228 Page61of99
September/2014
7.10 MainControlBoardDescription&Replacement
Toaccessthemaincontrolboardforfuseorboardreplacement,removescrewsateachuppersideofthecontrol
centerandallowthecontrolcentertorotatedown,restingonthesafetystraps.Nowthemaincontrolboardis
exposedforservice.
7.10.1 MainControlBoardReplacement
ThemaincontrolboardconsistsoftwointerconnectedPrintedCircuitBoard(PCB)assemblies.ThefrontPCB
containstheLCDdisplay.ThebackPCBcontainsthepowersupply,sensorinputs/outputsandcontrol
inputs/outputscomponents.ThemechanicalandelectricalinterconnectsforthetwoPCB'salloccurwithinthe
assembliesandarefastenedtogetherwithstandoffsandscrews.
7.10.2 MainControlBoardFuseReplacement
Disconnectelectricalpowerfromcabinetbeforefusereplacement.
AllACcircuitsarefuseprotectedandwhenreplacementisnecessary,USEONLYFUSESOFSAMETYPEANDRATINGFOR
PROTECTIONAGAINSTRISKOFFIRE.
DESCRIPTION: BLOWERFUSE OUTLETFUSE LIGHTFUSE POWERINPUTFUSE
OM0228 Page62of99
September/2014
7.10.4 CabinetReset
Themaincontrolboardhastwosoftwareoperatingresetsavailableforqualifiedservicepersonnel.
Thetwotypesarethefollowing:
FactoryResetResetssetpointsandselectedoptionsettings.Factoryresetshouldbeusedintheeventthe
systemmemorydevelopsanerrorinoperation.Cabinettype,motortypeandcalibrationdata
willnotbeaffectedwiththisreset.
MasterResetResetsallcalibration,cabinettype,motortype,sensordata,andoptionssettingsbackto
defaultsettings.MasterResetshouldonlyneedtobeusedforamaincontrolboard
replacement.
AfterpressingtheCalibration/Servicemenuitem,apasswordscreenwillappear.Thedefaultpasswordis9876.
Oncetheservicepasswordisentered,theCalibration/Servicemenuwillappear.
MENU
0 5 UV
FL EXIT
1 6
ENTER PASSWORD
2 7 XXXX BACK
3 8
ENTER
4 9
xx:xx AM xx/xx/xx
SelectCABINETRESETfromthemenu.
MENU
UV
FL
SETPOINTS/LIMITS UP
SENSOR SETUP
NIGHT SETBACK
SERVICE DOWN
OPTION SETUP
DIAGNOSTICS
CABINET RESET ENTER
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page63of99
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Selectdesiredfunctionfrommenu.
MENU
UV
FL
UP
FACTORY RESET
DOWN
MASTER RESET
ENTER
xx:xx AM xx/xx/xx
Performeitherresetfunctionasselectedbelow.
MENU
UV
FL
FACTORY RESET
RESET
xx:xx AM xx/xx/xx
Oncefactoryresetiscomplete,returntoCalibration/Servicemenutoenteranyoptions.
MENU
UV
FL
MASTER RESET
RESET
PRESS RESET TO INITIATE A MASTER
RESET. THIS WILL CAUSE ALL
CALIBRATION DATA AND USER SETTINGS
TO BE LOST!!
PRESS MENU TO EXIT WITHOUT CHANGE.
xx:xx AM xx/xx/xx
OncetheMASTERRESETiconispressed,thedisplayscreenwillremainthesameforapproximately1minute.Also
duringthis1minute,anaudiblesignaloftheresetprocesswilloccur.Oncetheresetprocessiscompletethe
displayscreenwillrevertbacktotheNuAirelogomainmenu.AtthispointthecabinetMUSTbeturnedoffto
completetheprocess.Eitherunplugthecabinetorusethepowerswitchwithinthecontrolcentertoturnoffthe
cabinet.Turnthecabinetbackonthedisplayscreenwillremainblankforuptoaminute,thenwillindicate
PowerLossAlert,pressthescreentoclearthemessageandreturntotheCalibration/Servicemenutoenter
cabinettype,motortype,verifysetpoints,findsensorsandperformairflowcalibration.
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7.11 DigitalAirflowSensorDescription&Replacement
7.11.1 DownflowVelocitySensor
Theairflowsensorfunctionutilizestwothermistorsthatprovideaconstantcurrentsource.Onethermistorisa
referencethatusesaverylowcurrentsource.Theotherthermistoristheairflowmeasurerthatusesaveryhigh
currentsource.Asairflowpassesoverthethermistors,theairflowremovesheatfromthethermistermeasuring
airflow.Thelossofheatfromthethermistorcausesthevoltagefromthethermistortoincrease.Thisincrease
subtractedfromthereferencethermistoroutputvoltageiswhatdirectlyrelatestoairflowvelocity.Arepeatable
curvecanbegenerated(voltagevs.airflowvelocity).
Thethermistorsusedareglassbeadandcoatedandcanbecleanedbygentlyusingacottonswabandalcohol.
Formaldehydegas,HydrogenPeroxideandChlorineDioxidehasnoeffectontheairflowsensors;however,the
formaldehyde/Ammoniumbicarbonateresiduethatremainsafterdecontaminationshouldberemovedfromthe
airflowsensorthermistors.
Disconnectelectricalpowerfromthecabinetbefore
! CAUTION attemptinganymaintenanceaction.
Theairflowsensorisremovedbyturningthelockingringcounterclockwiseandgentlypullingthesensoraway
fromtheconnector.Toreattachtheairflowsensor,turnsensorinkeyedconnectoruntilkeymatches,pushinand
turnthelockingringclockwiseuntilringlocks.
Oncethenewsensorhasbeenreplaced,proceedtothedigitalsensorsetupprocedureinsection8.
7.11.2 ExhaustPressureSensor
Theexhaustpressuresensorisadigitaldifferentialvelocitypressureflowgrid.Theexhaustpressuresensorboard
islocatedwithintherightsideofthecontrolcenter.
Theexhaustpressuresensorfunctionutilizesadifferentialpressuretransducerandelectronictemperaturesensor
tomeasureexhaustvolume.Aflowgridmountedintheexhaustairflowstreamprovidesavelocityandstatic
pressuretothedifferentialpressuretransducer.Anequationusingthepressuredropacrosstheflowgridalong
withthetemperaturereadingprovidesavolumetricflowreading.Thevolumetricflowreadingisthencalculated
bytheductareatoprovidethedisplayedexhaustvolume.
TheflowgridlocatedintheexhaustairstreamismadefromPVCandisnotaffectedbyFormaldehyde,Hydrogen
PeroxideorChlorineDioxide.
Disconnectelectricalpowerfromthecabinetbefore
! CAUTION attemptinganymaintenanceaction.
Theexhaustpressuresensorboardisremovedbyunfastening(2)6/32nutsandremovingtheconnectorsand
tubingthengentlypullinguptheboarduntilfree.Toreattach,reversetheaboveprocedure.Theexhaustsensor
alsohasanonboardLEDindicatorthatindicatesaproperlyoperatingsensor.TheLEDDS1blinksinslow1second
intervalsduringnormaloperation.Itwillblinkfasterorfullonwhenasensorerroroccursatwhichtimethe
sensorboardneedstobereplaced.Oncethenewsensorhasbeenreplaced,proceedtothedigitalsensorsetup
inSection8.
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8.0 ErrorMessages,Troubleshooting,OptionDiagnostics&AirflowSensorPerformanceVerification
Audiblealarmsanderrormessagesoccurforavarietyofreasons.Wheneveranalarmconditionhasbeenpresentfora
periodofatleast10seconds,theaudiblealarm/errormessagewillbepresentedandstayonuntiltheerroriscleared.The
audiblealarmwillbeonfor30secondsuponinitialalarmcondition,thenonceeverytenseconds.Whenpresentedwithan
errormessage,pleaseperformthefollowing:
Step1: NOTEALLERRORMESSAGES.
ErrormessagewillappearinplaceiftheNuAirelogowithActiveAlarmsandthealarmtypebelow.
Step2: VERIFYERRORMESSAGES.
Errormessagescanbeverifiedbycleaningtheerrorfunctionbyeitherturningtheblower
orthecabinetonandoff.
Step3: MONITORREOCCURRENCEOFERRORMESSAGES.
Ifreoccurrenceoftheerrormessageisimmediateordaily,useguidebelowtocorrectthesituation.
OM0228 Page66of99
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8.1 ErrorMessageTroubleshootingGuide
ErrorMessage Indicator Correction
WindowAlarm Slidingwindowisaboveitsstandardworking Verifystandardworkingheightandwindowmicro
(WindowHigh) heightormicroswitchisnotoperatingproperly. switchoperation.
AirflowAlarm
Downflowairflowfellbelowitslowerlimitalarm
RedDownflowArrow Recertifycabinettoproperairflowsetpoints.
setpoint.
(DownflowLowLimit)
AirflowAlarm
Downflowairflowwentaboveitshighalarm
RedDownflowArrow Recertifycabinettoproperairflowsetpoints.
setpoint.
(DownflowHighLimit)
AirflowAlarm
Inflowairflowfellbelowitslowerlimitalarm Checkorientationofexhaustsensorshroud.
RedInflowArrow
setpoint. Recertifycabinettoproperairflowsetpoints.
(InflowLowLimit)
AirflowAlarm
Checkorientationofexhaustsensorshroud.
RedInflowArrow InflowAirflowwentaboveitshighalarmsetpoint.
Recertifycabinettoproperairflowsetpoints.
(InflowHighLimit)
LowPressureAlarm
Indicateslowpressureorlowcabinetairflow Recertifycabinettoproperairflowsetpoints.
(LowpressureLimit)
Checklightfuseonmaincontrolboard.
Checkfluorescentlamps.
Cabinetfluorescent Checkvoltagecomingoutofmaincontrolboardto
lightswon'tturnon. lightballasts.
Checklightstarters,ifpresent.
Checkballast.
Checkblowerfuseonmaincontrolboard.
Checkvoltagecomingoutofmaincontrolboard.
Checkwiringtoblower.Checkblowermotor.
CabinetBlower
(Note:blowermotorhasinternalthermal
won'tTurnOn.
protector.Letblowermotorcooloffforaminimum
of30minutestoassurethermalprotectorisnot
open).
Displayindicates
Indicatesthattheremoteoverrideisactivated,
(RemoteOverride
preventingtheusageofthecabinet.
Active)
PowerLossAlert! Indicatesapowerinterruptionhasoccurred. PressDISPLAYtoclearmessage.
Cabinetoutletswon't Checkoutletfuselocatedonmaincontrolboard.
turnon. Checkvoltagecomingoutofmaincontrolboard.
Checkslidingwindowpositionsothatit'sfully
closed.
Cabinetultravioletlight Checkblower/lightsfuseonmaincontrolboard.
won'tturnon. Checkvoltagecomingoutofthemaincontrolboard
toultravioletlightballast.
Checklightstarters,ifpresent.Checkballast.
Blowerorlightfuse Checkforshortonoutputoffuse.
continuestoblowafter Isolateoutputoffusebydisconnectinglightcircuit,
replacement. blowercircuit,etc.toisolateshort.
ReplaceUVLight! IndicatesthattheUVlightneedsreplacement ReplaceUVlightandclearUVruntimeclock.
Displayindicates Indicatesthatthenightsetbackisactivated,
(NightSetbackActive) preventingtheusageofthecabinet.
Indicatestheneedtoschedulethecabinet
CertificationdueinX Messagewillclearoncethecertifierupdatesthe
certificationbasedonthescheduledtimetable
weeks. currentcertificationdate.
programmed.
ActiveAlarms Checkconnectorsandwiresfrommaincontrol
Indicatesadigitalcommunicationserrorfromthe
DNSensorComm! boardtotheairflowsensors.DNindicated
maincontrolboardtotheairflowsensors.
EXSensorComm! downflowsensor.EXindicatesexhaustsensor.
ActiveAlarms Checkairflowprobeconnectoronmainboard.
Indicatesanerrorsignalgeneratedbythesensor.
DNSensorError! (Ref.Section7.11).Replaceairflowsensorif
OM0228 Page67of99
September/2014
EXSensorError! required.
8.2 Calibration/ServiceMenu
8.2.1 General
Aswiththeairflowcalibrationprocess,theservicemenushouldonlybeaccessedbyaService
Technicianthatisfamiliarwiththeproduct.PressMENUtoaccessCalibration/Serviceparameter.
MENU
UV
FL
CALIBRATION/SERVICE UP
TIME/DATE
PASSWORD DOWN
CABINET DECONTAMINATION
ENTER
SCREEN SET-UP
FILTER STATUS
xx:xx AM xx/xx/xx
EntryintotheCalibration/Servicefunctionsrequiresaservicepasswordforentry.Afterpressingthe
Calibration/Servicemenuitem,apasswordscreenwillappear.Thedefaultpasswordis9876.
Oncetheservicepasswordisentered,theCalibration/Servicemenuwillappear.
Asaspecialfeaturefortheservicetechnician,byaccessingtheCalibration/Servicemenu,theservicetechnician
canbypasstheblowerwarmuptimeitself.Thisfeatureremainsonforonehourfromthetimethe
Calibration/Servicemenuwasaccessed.Whenbypassingthewarmuptime,itisnotuncommontoexperiencea
briefalarmastheblowerstabilizesatsetpoint.
0 5 MENU
EXIT
UV
FL
1 6
ENTER PASSWORD BACK
2 7 XXXX
3 8 ENTER
4 9
xx:xx AM xx/xx/xx
8.2.2 Calibration/ServiceMenu
TheCalibration/Servicemenuprovidesalistofsubmenuitemstoaccomplishallservicetasks.
Eachsubmenuitemwillbedescribedinthefollowingsections.
MENU
UV
FL
UP
SETPOINTS/LIMITS
SENSOR SETUP
NIGHT SETBACK DOWN
SERVICE
OPTION SETUP
DIAGNOSTICS ENTER
CABINET RESET
CABINET TYPE
DAMPER CALIBRATION
xx:xx AM xx/xx/xx
OM0228 Page68of99
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8.2.3 CabinetType/MotorType
Thecabinettypecanbeverifiedinthecontrolsystemandisfactorysetandshouldntrequirealteration.The
cabinettypedefaultinformationcontrolsunitofmeasure,setpointsandlimitsbasedonthetypeandsizeofcabinet.
Toverify,pressCABINETTYPE.Thecurrenttypeofcabinetwillbedesignated.Againtoverify,pressthecorrect
TypeandtheModel/Sizeofthecurrentselectedcabinetmodelwillbedesignated.Onceverifiedthecorrecttype
andmodelaredesignated,thenpressBACKtoreturntoCalibration/Servicemenu.
CURRENTLY B2 NU-430/435-400
MENU
UV
FL
xx:xx AM xx/xx/xx
B2 NU-430/435-300 UP
B2 NU-430/435-400
B2 NU-430/435-500
B2 NU-430/435-600 DOWN
B2 NU-430/435-800
B2 NU-430-400(E)
SAVE
B2 NU-430-600(E)
B2 NU-430-400(D)
B2 NU-430-600(D)
MORE
xx:xx AM xx/xx/xx
PressSETMOTORTYPEtoverifycorrectsetting.UponaMASTERRESET,themotortypeisdefaultedtoAC.The
NU430Series60requiresthemotortypeselectedtobeDC.Alwaysverifymotortypewhenverifyingcabinet
type.
OM0228 Page69of99
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IftheLFBSChasaspecialexhaustductdiametersizeorspecialworkaccessopeningwindowheight,thesemustbe
enteredintothecontrolsystemtoassurethecorrectdisplayvalues.PressMENUtoaccesstheseadditional
parameters.SelectandSAVEappropriatesizeofeach.
MENU
UP
SET EXHAUST DIAMETER SIZE
PREVIOUS
SAVE
xx:xx AM xx/xx/xx
UP
14 INCH EXHAUST
10 INCH EXHAUST
SAVE
8 INCH EXHAUST
xx:xx AM xx/xx/xx
UP
8 INCH
10 INCH DOWN
12 INCH
SAVE
xx:xx AM xx/xx/xx
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8.2.4Setpoints/Limits
Theairflowsetpointsandalarmlimitsmayalsobeverifiedoraltered.Typicallythesedefaultvaluesarefactoryset
basedonthecabinettype,modelandsizeaspreviouslydiscussed.However,theymaybealteredinspecialcases
formodifiedcabinets.Thesetpointestablishestheairflowvaluesthataretobemaintained.Thehighlowlimits
establishthealarmboundariesfromthenominalsetpoint.Thedefaultvalueshavebeenestablishedbasedupon
theperformancespecificationsandcabinetcomponenttolerances.
Toverifyoralteranyoftheairflowsetpointsoralarmlimits,pressthemenusetpoints/limitsmenuitem.Then,
pressanyoftheindividualsetpointsoralarmlimitstoverifyand/orchange.PressUPorDOWNtochangenew
value.PressSAVEtoenternewvalue.
MENU
UV FL
UP
DOWNFLOW SETPOINT
DOWN
DOWNFLOW LOW LIMIT
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
OLD DOWNFLOW SETPOINT 60 fpm
xx:xx AM xx/xx/xx
DefaultvaluesforNU430400 DefaultvaluesforNU430600
Downflowsetpoint60 Downflowsetpoint60
Downflowhighlimit66 Downflowhighlimit66
Downflowlowlimit54 Downflowlowlimit54
Exhaustsetpoint754 Exhaustsetpoint1100
Exhausthighlimit867 Exhausthighlimit1265
Exhaustlowlimit641 Exhaustlowlimit935
OM0228 Page71of99
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8.2.5 DigitalSensorSetup
Thedigitalsensorsetupmenuisusedforbothcalibrationandsensorreplacementifnecessary.Forsensor
calibrationprocess,seeairflowcalibrationsection.Forsensorreplacement,usetheFindnewdownflow/
exhaustmenusbelow.
Ifreplacingbothsensors,thedownflowsensorwillalwayshavetobeconnectedandfoundfirst.Toreplaceoneor
bothsensors,performthefollowingprocedure:
Disconnectpowertocabinet.
NOTE:Apowerswitchisavailableinthecontrolcentertoturnthecabinetonandoff.
Connectionanddisconnectionmustalwaysbeperformedwiththecabinetpoweroff.
NOTE: Theexhaustpressuresensorhasaseparatecalibrationboard.
Thedownflowsensordoesnothaveaseparatecalibrationboard.
Reconnectdownflowsensorifnotconnectedanddisconnectexhaustpressuresensor.
Turnonpowertocabinetandnavigatetothesensorsetupmenu
SelectFINDNEWDOWNFLOWSENSOR,andfollowmenu
PressLEARNtofindsensor.Ifsuccessful,displaywillindicateSENSORFOUND,ifnot,displaymay
indicateSENSORALREADYUSEDorFAILEDTOFINDSENSOR.Ifthisisthecase,performMASTER
RESETandstartprocessover.
MENU
UV
FL
UP
CALIBRATE DOWNFLOW SENSOR
CALIBRATE EXHAUST SENSOR DOWN
FIND NEW DOWNFLOW SENSOR
ENTER
FIND NEW EXHAUST SENSOR
xx:xx AM xx/xx/xx
MENU
UV
FL
CONNECT DOWNFLOW SENSOR TO THE
CABINET AND REMOVE EXHAUST FLOW SENSOR.
LEARN
PRESS LEARN TO FIND SENSOR.
PRESS THE LEARN KEY WHEN READY.
xx:xx AM xx/xx/xx
Oncedownflowsensorisfound,turnoffpower,reconnectexhaust&pressuresensor,turnonpower
andproceedimmediatelytoairflowcalibrationsections(i.e.calibratedownflowsensor).
NOTE: OntypeB1/B2cabinets,itisnotnecessarytofindtheexhaustsensorsinceitisadifferenttype
fromthedownflowsensorandthemaincontrolboardwillautomaticallyrecognizeit.
OM0228 Page72of99
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8.2.6 NightSetback
Theoptionalnightsetbackfeatureisusedtoreducetheexhaustairvolumeduringnonusageperiodsresultingin
conditionedairenergysavings.Forthenightsetbacktooperate,acontrolvalvemustbeinstalled(i.e.NU951
012motorizedairtightbutterflyvalve)toprovidethemeansforreductionofexhaustairflow.
Thenightsetbackisinitiatedbyeitherclosingthecontactsonthemaincontrolboardorenablingthenight
setbackicononthedisplay.Ifbothareused,thecontactsonthemaincontrolboardhavepriorityoverthedisplay
icon.Oncethecontactisclosedoriconispressedtheinternalblowerandfluorescentlightswillbeturnedoffand
remaininoperable.Theexhaustvalvewillbeclosedtoapercentageoftheoriginalsetpointtypicallytomaintaina
minimumof100fpm(.51m/s)andthedisplaywillindicatenightsetbackactive.Theslidingwindowcanbeclosed
andtheUVlightturnedonifinstalled.
MENU
UV
FL
NIGHT SETBACK
SETBACK = 80 PERCENT UP
xx:xx AM xx/xx/xx
8.2.7 Service
TheservicesetupmenuallowsaQUALIFIEDTECHNICIANtoconfigure,calibrateandobtainfunctionalservicedata.
Eachparametersubmenuwillbedescribedaswellasthedisplaywillindicatepresentand/ordefaultconditionsas
shown.
MENU
UV
FL
RUN TIMES UP
MAIN COMPONENT REPLACEMENT
SERIAL NUMBER
CERTIFICATION DATE DOWN
FILTER STATUS DATA
ENTER
PRESS MENU TO EXIT.
xx:xx AM xx/xx/xx
OM0228 Page73of99
September/2014
RunTimes
Thisparameterallowstheservicetechniciantoview,alter,orresetbothUVlightandmotorblowerruntimer.
Selectdesiredruntimeparameterfrommenu.
MENU
UV
FL
UP
UV RUN TIME
DOWN
ENTER
xx:xx AM xx/xx/xx
UVRunTime.
MENU
UV
FL
UV LIGHT USAGE
RESET
CURRENT HOURS = XXXX
MotorBlowerRunTime.
MENU
UV TIME
MOTOR BLOWER RUN UP
FL
CURRENT HOURS = XXXX
DOWN
THE MOTOR BLOWER RUN TIME INDICATES HOW
MANY HOURS THE BLOWER HAS BEEN ON. TIME
MAY BE RESET OR TRANSFERRED FOR SERVICE SAVE
PURPOSES.
PRESS UP, DOWN TO CHANGE THE HOURS.
xx:xx AM xx/xx/xx
OM0228 Page74of99
September/2014
MainComponentReplacement
ThisparameterallowstheservicetechniciantoviewandupdateHEPAfilters,blowermotor,maincontrolboard
andsensorinstallationdates.PressENTERtoselectitem,thenenterdateinfollowingscreen.
MENU
UV
FL
UP
SUPPLY HEPA FILTER MM / DD / YYYY
EXHAUST HEPA FILTER MM / DD / YYYY
MENU
UV
FL
UP
SETTING__________ ____________ REPLACEMENT DATE
<<
MM / DD / YYYY DOWN
>>
SAVE
PRESS >> AND << TO SELECT MONTH, DAY OR YEAR.
PRESS UP/DOWN TO CHANGE VALUE.
PRESS SAVE TO ENTER NEW SETTING.
xx:xx AM xx/xx/xx
SerialNumber
Thisparameterallowstheservicetechniciantoviewandenterthecabinetsserialnumber
MENU
UV
FL
SERIAL NUMBER
XXXXXXXXXXXX CHANGE
xx:xx AM xx/xx/xx
OnceCHANGEispressed,adisplayscreenverysimilartothepassworddisplayscreenwillappearforentryof
theserialnumber.AfterentryoftheserialnumberpressENTERtosavetheserialnumber.
OM0228 Page75of99
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CertificationDate
Thisparameterallowstheservicetechniciantoviewandupdatethecurrentcertificationdate.Thecertification
datealsohasafeaturetoindicateanadvancenoticethatrecertificationisdue.PressDUEtoenterintothe
certificationrequiredtimeperiodscreen.Selectdesiredintervalofcertificationrequired,i.e.NoReminder,6,12,
18,or24months.Thereminderwillappearduringthewarmupcyclefor10secondseverytimethebloweris
turnedonstarting4weeksbeforetheduedate,thenpastdue.
MENU
UV
FL
xx:xx AM xx/xx/xx
OM0228 Page76of99
September/2014
8.2.8 OptionSetUp
TheoptionsetupmenuallowsAQUALIFIEDTECHNICIANtoconfigureseveraldifferentoptionalparametersper
themenubelow.Eachparametersubmenuwillbedescribedaswellasthedisplaywillthedefaultconditionsas
shown.
MENU
UV
FL
AIRFLOW DISPLAY AVERAGING
UV WINDOW INTERLOCK UP
CONTROL/ALARM MANUAL CONTROL
ALARM SILENCE
EXHAUST OVERRIDE DOWN
MENU
UV
FL
EXHAUST AIRFLOW CONTROL UP
REMOTE OVERRIDE DAMPER
BLOWER WINDOW INTERLOCK
AUX RELAY FUNCTION DOWN
PRINTER REPORT
BLOWER FL LIGHT INTERLOCK
FAN RELAY INTERLOCK
ENTER
ALARM SILENCE TIME
DOWNFLOW UPDATE TIME
DOWNFLOW ALARM TIME
MORE OPTIONS
xx:xx AM xx/xx/xx
AirflowDisplayAveraging
Thisparameterallowsfortheselectionoftheairflowdisplayaveragingfunctiontooperate.Whentheairflow
averagingison,thedownflowdisplaywillalwaysindicatetheairflowsetpoint60fpm(0.30m/s),iftheairflowis
validandwithinitsalarmlimits.Theexhaustdisplaywillusetheexhaustupdateaveragingfunction.
MENU
UV
FL
ON
xx:xx AM xx/xx/xx
OM0228 Page77of99
September/2014
UVWindowInterlock
ThisparameterallowsfortheselectionofthewindowclosedswitchtobeinterlockedwiththeUVlightoption.
Whenthewindowinterlockison,thewindowmustbeclosedfortheUVlighttooperate.Whenthewindow
interlockisofftheUVlightcanbeturnedonregardlessofwindowposition.
NOTE:InadditiontotheTOUCHLINKsystemUVwindowinterlockthereisadoubleredundantUVwindow
interlockrelay.TooverridetheUVwindowinterlockforservicepurposesonly,bothinterlocksmustbechanged
throughtheTOUCHLINKsystemandshortingtherelayconnection(seeelectricalschematicforreference).
MENU
UV
FL
ON
WINDOW INTERLOCK
OPTION IS ON
OFF
WHEN THE WINDOW INTERLOCK IS ON, THE
WINDOW MUST BE CLOSED FOR THE UV LIGHT TO
SAVE
OPERATE. WHEN OFF, THE WINDOW CAN BE IN
ANY POSITION.
xx:xx AM xx/xx/xx
Control/AlarmManualControl
ThisparameterallowsONLYTHECABINETTECHNICIANtorunthecabinetinmanualmode.Thismeanswithno
controlsoralarmsactivated.Whenthemanualcontrolison,thedownflowandinflowdisplayswillindicate
nominalsetpoints.Airflowadjustmentscanbemadeinthemanualmodebygoingintoairflowcalibrationand
adjustingtheblowerdutycycle.Theblowerdutycyclewillremainconstantinmanualmode.Thedisplaywillalso
indicatethemanualcontrolisactivated.Whenthemanualcontrolisoff,fullautomaticcontrolresumes.
MENU
UV
FL
xx:xx AM xx/xx/xx
OM0228 Page78of99
September/2014
AlarmSilence
Thisparameterallowsfortheselectionofthealarmsilencekeyfunction.Whenthealarmsilencefunctionison,
allcurrentandfuturealarmswillbesilencedforthedesignatedalarmsilencetime(i.e.defaulttimeis15minutes).
Whenthealarmsilencefunctionisoff,allcurrentalarmswillbesilencedforthedesignatedalarmsilencetime.If
anewalarmispresent,theaudiblealarmwillagainbeturnedon.
MENU
UV
FL
xx:xx AM xx/xx/xx
ExhaustOverride
CAUTION:THISPARAMETERSHOULDALWAYSBETURNEDOFFFORSAFEFUNCTIONOFATYPEB2CABINET.
Thisparameterallowsfortheselectiontooverridethecontrollerblowerinterlockfortestingpurposes.When
exhaustoverrideisoff,sufficientexhaustairflowisrequiredfortheinternalblowertofunction.Whenonthe
internalblowerwillfunctionwithoutsufficientexhaust.
NOTE:Theredundantpressureswitchmustalsobebypassedfortheinternalblowertorunwithoutexhaust.
MENU
UV
FL
EXHAUST OVERRIDE
OPTION IS OFF ON
WHEN EXHAUST OVERRIDE IS OFF,
OFF
SUFFICIENT EXHAUST AIRFLOW IS REQUIRED FOR
THE BLOWER TO FUNCTION.
WHEN THE EXHAUST OVERRIDE IS ON, THE BLOWER SAVE
WILL FUNCTION WITHOUT SUFFICIENT EXHAUST.
xx:xx AM xx/xx/xx
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September/2014
MotorBlowerLockout
Thisparameterallowstheaccesstoturnthebloweronorofftoberestrictedbytheuseofapassword.Whenthe
blowerlockoutison,pressingtheblowericonwillproduceanumericalpasswordscreen.Thedefaultpasswordis
1234andmaybechangedusingthepasswordmenu.Whentheblowerlockoutisoff,theblowermaybeturned
onandoffwithoutrestriction.
MENU
UV
FL
ON
BLOWER LOCKOUT
OPTION IS OFF
OFF
xx:xx AM xx/xx/xx
ExhaustControlDamperPosition
Thisparameterallowsfortheselectiontodisabletheexhaustcontrol/alarmandselectadesireddamperposition
whenthebloweristurnedoff.Normallywhenthebloweristurnedofftheexhaustcontrolandalarmfunction
remainsactiveintheB1/B2configuration.However,ifthecabinetistiedintotheexhaustblowercircuititis
desirabletoturnofftheexhaustcontrolandalarmfunctionwhentheblowerswitchisturnedoff.Thiswillavoid
havingaconstantexhaustalarmwhentheexhaustsystemisturnedoff.Validsettingsfortheexhaustcontrol
damperpositionareopen,close,freeze,oroff.Offkeepstheexhaustcontrol/alarmsactivewhenthebloweris
off.
MENU
UV
FL
xx:xx AM xx/xx/xx
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ExhaustControlAutoZero
ThisparameterallowstheexhaustcontrolsystemwhenusingtheNU951AutomaticDampertoperformacheck
fromitsoriginalcalibrationpointonceevery24hours.Duringtheairflowcalibrationprocedure,oncethecertifier
calibratestheexhaustvolumethecontrolsystemwillrememberthedamperpositionindegreesatthatpoint.
Thenbasedontheprogrammedtime,theexhaustcontrolsystemwillperformthefollowingautozerosequence.
First,freezetheexhaustdisplaytoitssetpoint.Thenmovethedamperbacktoitsoriginalcalibrationpoint.Allow
thedampertocontrolnormallyfortwominutesthenunfreezethedisplay.Thissequencewillthenbeperformed
attheprogrammedtimeonceevery24hours.TheautozerosequencecanbecheckedbypressingTESTtoinitiate
afourminutetestsequence.
MENU
UV
FL
ON
xx:xx AM xx/xx/xx
ExhaustUpdate
Thisparameterallowsfortheselectionoftimetodeterminehowmuchexhaustflowdataisaveragedbefore
beingdisplayed.Thetimeisdisplayedinsecondswithaprogrammablerangeof1to60.
MENU
UV
FL
EXHAUST UPDATE UP
OPTION = 30
DOWN
THIS OPTION DETERMINES HOW MANY
SECONDS OF EXHAUST DATA IS
AVERAGED BEFORE BEING DISPLAYED. SAVE
VALID RANGES ARE 1 TO 60 SECONDS.
xx:xx AM xx/xx/xx
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ExhaustAirflowControl
Thisparameterallowsfortheselectionoftime,programmablefrom10to30secondsdetermininghowoftenthe
exhaustcontrollerupdatesthecontrolsignaltotheautomaticdamper(i.e.NU951012)ifinstalled.Reducingthe
timewillcausethedampertoreactquickertochange,butmayresultincontrolovershootandoscillations.
Increasingthetimewillcausethedampertoreactslowertochanges,butmaynotkeepupwithnormalsystems
fluctuations.DependingupontheHVACsystem,changingthisparameterallowscontrolflexibilityforexhaust
systemstabilityoptimization.
MENU
UV
FL
xx:xx AM xx/xx/xx
RemoteOverrideDamperOpen
ThisparameterallowsortheNU951dampertoopenduringaremoteoverridecontactclosure.
NormallytheNU951damperfunctionwillcloseduringaremoteoverridecontactclosure.
However,thisoptionallowstheoppositedamperfunction.
MENU
UV
FL
xx:xx AM xx/xx/xx
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BlowerWindowInterlock
Thisparameterallowsfortheselectionofthewindowclosedswitchtobeinterlockedtotheblower.Whenthe
blowerinterlockison,theblowerwillturnoffwhenthewindowisclosed.Whentheblowerisoff,theblowerwill
continuetorunwhenthewindowisclosed.
MENU
UV
FL
ON
BLOWER WINDOW INTERLOCK
OPTION IS ON OFF
CONTINUE TO RUN.
xx:xx AM xx/xx/xx
AUXRelayFunction
ThisparameterallowsfortheselectionoftheAUXrelayfunction.WhentheAUXrelayison,theAUXrelay
functionwillbeidenticaltothefanrelay.WhentheAUXrelayfunctionisoff,theAUXrelayfunctionprovides
delayOn/Offoption.
MENU
UV FL
ON
AUX RELAY FUNCTION OPTION IS ON
OFF
WHEN AUX RELAY FUNCTION IS ON, THE RELAY
FUNCTION WILL BE IDENTCAL TO THE FAN RELAY.
WHEN OFF THE RELAY CAN BE USED FOR EXHAUST
DELAY ON/OFF OPTIONS. SAVE
xx:xx AM xx/xx/xx
PrinterReportFrequency
ThisparameterallowsfortheselectionofthefrequencyoftheRS232CommunicationOutput.The
CommunicationOutputprovidescommunicationfromthecabinettoapersonalcomputer(HyperTerminal)or
printerviaRS232interconnectstandard.RS232providesshortrange(50feet)simplepointtopointconnection
withanotherRS232device.
TheCommunicationInterfaceutilizes9bitcharacterframewitheightbits(noparity)andastopbit(8.N.1)witha
constanttransmissionspeedof57,600bpsforcommunication.
TheconnectionfortheRS232outputislocatedonthemaincontrolboardasaRJ45(8pin)connector(J5).
Thefollowingisthepinpositionfortheconnector:
1NOTUSED 5NOTUSED
2NOTUSED 6NOTUSED
3GROUND 7TRASMIT
4NOTUSED 8RECEIVE
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TheCommunicationOutputprovidesairflowreadingsasshownbelow.
Actualairflowswithsetpoints(inparentheses)areoutput.
MENU
UV
FL ON
PRINTER REPORT FREQUENCY
OPTION SETTING = 0
OFF
THIS OPTION DETERMINES HOW OFTEN THE
CONTROLLER OUTPUTS THE CURRENT STATUS TO A
PRINTER. VALID CHANGES ARE 1 TO 60 MINUTES. SAVE
xx:xx AM xx/xx/xx
BlowerFLLightInterlock
Thisparameterallowsfortheselectionofthefluorescentlightoptiontobeinterlockedtotheblower.Whenthe
blowerFLlightinterlockison,thefluorescentlightoperationwillbeinterlockedtotheblower.Whentheblower
FLlightinterlockisoff,thefluorescentlightcanbeturnedonatanytime.
MENU
UV
FL
ON
BLOWE FL LIGHT INTERLOCK
OPTION IS OFF
WHEN BLOWER FL LIGHT INTERLOCK IS ON, THE FL OFF
LIGHT WILL TURN ON WHEN THE BLOWER IS
TURNED ON AND CAN ONLY BE TURNED ON WHEN
THE BLOWER IS ON. WHEN OFF, THE FL LIGHT CAN SAVE
BE TURNED ON AT ANYTIME.
xx:xx AM xx/xx/xx
FanRelayInterlock
Thisparameterallowsfortheselectionofthefanrelayinterlockoperation.Whenthefansrelayinterlockison,
andtheblowerswitchispendingorblue,thefanrelaywillbeoffornotenergized.Ifthefanrelayinterlockisoff
andtheblowerswitchispendingorblue,thefanrelaywillbeonorenergized.Ineithercasethefanrelaywillbe
onwhentheblowerswitchisonorgreenandoffwhentheblowerswitchisoffornotcolored.
MENU
UV
FL
ON
FAN RELAY INTERLOCK IS OFF
OFF
xx:xx AM xx/xx/xx
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AlarmSilenceTime
Thisparameterallowsfortheselectionoftimetodeterminehowlongtheaudiblealarmshallbesilenced.Thetime
isdisplayedinminuteswithaprogrammablerangeof1to60.
MENU
UV
FL
ON
ALARM SILENCE TIME
OPTION SETTING = 15
OFF
MENU
UV
FL
UP
DOWNFLOW UPDATE TIME
OPTION SETTING = 8
DOWN
THIS OPTION DETERMINES HOW MANY SECONDS OF
DOWNFLOW DATA IS AVERAGED BEFORE BEING
DISPLAYED. VALID RANGES ARE 4 TO 12 SECONDS. SAVE
xx:xx AM xx/xx/xx
DownflowAlarmTime
Thisparameterallowsfortheselectionoftimetodeterminehowmanycontinuoussecondsofanalarm
conditionoccursbeforeactivatinganaudibleandvisualalarm.Thetimeisdisplayedinsecondswitha
programmablerangefrom2to12seconds.
MENU
UV
FL
UP
DOWNFLOW ALARM TIME
OPTION SETTING = 8
DOWN
THIS OPTION DETERMINES HOW MANY CONTINOUS
SECONDS THE DOWNFLOW MUST BE IN ALARM
CONDITION BEFORE THE ALARM IS ACTIVATED. SAVE
VALID RANGE IS 2 TO 12 SECONDS.
xx:xx AM xx/xx/xx
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8.2.9 Diagnostics
ThediagnosticsmenuallowsAQUALIFIEDTECHNICIANtoexercisethecontrolsystemsinputsandoutputs.Each
ofthesehasitsownmenuscreentoexcisethecontrolsystem.Selectmenuscreenasdesired.
MENU
UV
FL UP
DOWN
TEST OUTPUTS
xx:xx AM xx/xx/xx
TestOutputs
Testoutputsallowaservicetechniciantoexercisetheseoutputfunctions.PressingTOGGLEwillturnonandoff
thefunctions.PressUPandDOWNtoselectthetestoutputdesired.Usethedisplayiconstoturnon/offeach
functionofblower,UVlight,fluorescentlight,outletandoptionalpowerwindowifinstalled.
MENU
UV
FL
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ReadInputs
Readinputsallaservicetechniciantoexerciseorchecktheseinputfunctions.Theinputsmaybecheckedby
alteringthestateoftheinputfunction(i.e.slidingwindowposition)andmonitoringthechangeonthedisplay.
MENU
UV
FL
KEYPAD X = 0, Y = 0
DOWNFLOW SENSOR 60 FPM
EXHAUST SENSOR 1100 CFM
REMOTE OVERRIDE OPEN
NIGHT SETBACK OPEN NEXT
WINDOW POSITION NORMAL
PRESSURE SENSOR ACTIVE
AC LINE HZ 60 HZ
AC LINE VOLTS 115 VAC
MOTOR RPM 1000 RPM
xx:xx AM xx/xx/xx
MENU
UV
FL
NEXT
WINDOW HIGH LIMIT CLEAR
WINDOW HIGH
CLEAR
WINDOW
CLOSED CLEAR
WINDOW
SAFE
TRIPPED
AM
xx:xx xx/xx/xx
8.3 AirflowSensorPerformanceVerification
Theindividualairflowsensorscanberoutinelycheckedduringcalibrationorindiagnosticstoassuretheyarereadingand
activewithintherangeofuse(0to200fpm)(0to1.02mps).Theairflowsensorscanalsobecheckedintherunmode
throughperformanceverification,forresponsivenesstochangingairflowconditions.
8.3.1 RunMode
Tochecktheairflowsensorinrunmode,firstallowthecabinettooperatenormallyforaminimumof5minutes.
Then,placearolledpieceofpaperoverthedownflowsensorintheworkzoneandleavethepaperonthesensor
foratleast2minutesandthenremove.Thisactionwillcausethecabinettogointoadownflowalarmcondition.
Theexhaustairflowreadingshouldincreaseduringthistest.However,thedownflowreadingshouldgodown
below25fpm(.13m/s).Thereshouldalsobeanoticeableincreaseinmotor/blowernoise.Itwouldalsobe
recommendedtomonitormotor/blowervoltageduringthetest.Themotor/blowervoltageshouldbemonitored
fromwhenthecabinetisrunningnormally.Duringthetest,whenthedownflowsensoriscovered,the
motor/blowervoltageshouldbesteadilyincreasingtoslightlyunderlinevoltage.Whenthedownflowsensoris
uncovered,themotor/blowervoltageshoulddecreaseandairflowreadingsshouldbewithinthecalibrationrange.
Ifthemotor/blowervoltagedoesnotchange,anairflowsensorproblemcouldexist.
PleaseconsultwithNuAireTechnicalService.
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9.0 RemoteContacts
TheNU430hasseveralcontactclosuresforremotesensingofvariousfunctions.
9.1 FanRelay
Thefanrelaycontactsaredualnormallyopencontactclosureoutputswhichareactivatedwheneverthebloweristurned
on.Contactratingsare250VACmaximumat2Amps.
9.2 AlarmRelay
Thealarmrelaycontactsaredualnormallyopencontactclosureoutputswhichareactivatedwheneveranairflowalarm
conditionoccurs.Anairflowalarmconditionwilloccurifeitherairflowsensordetects5consecutive2secondairflow
readingsaboveorbelowthealarmlimits.Contactratingsare250VACmaximumat2Amps.
9.3 AUXRelay
TheAUXrelaycontactsarecommon,normallyopenandnormallyclosedcontactclosureoutputswhichareactivated
wheneverthebloweristurnedon.However,theAUXrelaydoeshavesomeconditionallogicprogrammedtoaidthe
supplyOn/Offandexhaustdelayoptions.Therelaywillactivatewheneverthebloweristurnedonandstayonunlessafter
5minutesthereisalowexhaustalarm,thentherelaywilldeactivate.Ifexhaustissufficient,therelaywillstayactive.If
thebloweristhenturnedoff,therelaywillstayactiveforoneminute,thendeactivate.TheAUXrelaymayalsobe
selectedtooperatethesameasthefanrelay,referencetheAUXrelayfunctionintheoptionmenu.Contactratingsare
250VACmaximumat2Amps.
9.4 RemoteOverride
Theremoteoverridecontactsare(nopower)shortingcontactsonly,whichwhenclosed,indicatestothecontrolsystemto
shutdownthecabinet.Thedisplaywillindicateremoteoverride,inhibitallalarms,fluorescentlight,internalblowerand
closetheexhaustvalve(i.e.NU951)ifpresent.
9.5 NightSetback
Thenightsetbackcontactsare(nopower)shortingcontactsonly,whichwhenclosed,indicatestothecontrolsystemto
placethecabinetintonightsetback.Thedisplaywillindicatenightsetbackactive,inhibitexhaustalarms,fluorescentlight
andinternalblower.
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10.0 OptionalEquipment
10.1 UltravioletLight
Ultravioletlightwillinjureyoureyes.Avoiddirectviewingatalltimes.
! CAUTION
Personnelshouldnotbepresentwhenultravioletlampison
10.1.1 Overview
Thegermicidalultravioletisprimarilyintendedforthedestructionofbacteriaandothermicroorganismsintheair
orondirectlyexposedsurfaces.Approximately95%oftheultravioletradiationsfromgermicidaltubesareinthe
253.7nanometerregion.Thisisaregionintheultravioletspectrumwhichisnearthepeakofgermicidal
effectiveness.Theexposurenecessarytokillbacteriaistheproductoftimeandintensity.Highintensitiesfora
shortperiodoftime,orlowintensitiesforalongerperiodarefundamentallyequalinlethaldosageonbacteria
(disregardingthelifecycleofbacteria).Theintensityoflightfallingonagivenareaisgovernedbytheinverselaw;
thatisthekillingintensitydecreasesasthedistanceincreasesfromthetube.
Thegermicidaltubeisplacedinthecabinettoprovideanaverageintensityof100microwattspersquare
centimeter(foranewtube)fallingonahorizontalplanedefinedbythecenteroftheworksurface.The
minimumrequirementperparagraph5.12ofNSFStandard49is40microwattspersquarecentimeter(ref.NSF
Std.#49,June,1976).
Sinceultravioletrayswillnotpenetrateordinaryglass,itisrecommendedthattheslidingwindowbeclosedwhile
theultravioletlightisonwithinthecabinet;orthatpersonnelleavethecabinetfacearea.
10.1.2 Operation
TheoperationoftheultravioletlightisaccomplishedbyclosingtheslidingwindowandpressingtheUVswitch
locationonthefrontpanel.Theslidingwindowisinterlockedtotheultravioletlightso,whentheslidingwindow
israised,theultravioletlightwillturnoff.
10.1.3 Precaution
Theraysfromgermicidaltubesmaycauseapainfulbuttemporaryirritationoftheeyesandreddeningoftheskin,
ifofsufficientlyhighintensity,orifexposurecoversaprolongedperiodoftime.Forthisreason,oneshouldavoid
directeyeandskinexposuretoultravioletlight.Ifexposurecannotbeavoided,itisnecessaryforpersonnelto
weareyegogglesorfaceshields,andlongsleevegownswithrubbergloves.
Sinceultravioletrayswillnotpenetrateordinaryglass,itisrecommendedthattheslidingwindowbeclosedwhile
theultravioletlightisonwithinthecabinet;orthatpersonnelleavethecabinetfacearea.
10.1.4 Maintenance
Theoutputofanultravioletlightdeteriorateswithburningage.Theusefullifeofthelightisapproximately7000
hoursunderspecifictestconditions.
NOTE:Beforetestingwithlampoff,thelightmaybecleanedwithalintfreeclothdampenedwithalcoholor
ammoniaandwater.
Itisrecommendedthateitheratimeschedulebeestablishedorthetube'soutputbemeasuredperiodicallyand
thetubereplacedwhenitsoutputfallsbelow40microwattspersquarecentimeterorexceeds7000hoursof
operation.Lightsshouldbeallowedtooperateapproximately5to10minutes(longerwhenthelightisinlow
temperatures)towarmupsufficientlybeforereadingtheoutputwithameter.
OM0228 Page89of99
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EnergiesRequiredtoDestroySomeMicroorganismsByUltravioletRadiations(e)
Microwatt Microwatt
MoldSpores seconds Protozoa seconds
percm/2 percm/2
Penicillumroqueforti 26,400 Paramecium 200,000(a)
Penicilliumexpansum 22,000
Penicilliumdigitiatum 88,000 NematodeEggs 40,000(b)
Aspergillusglaucus 88,000
Aspergillusflavus 99,000 Algae 22,000(c)
Aspergillusniger 330,000
Rhizopusnigricans 220,000 Virus
MucorracemosusA 35,200 Baceriophage(E.Coli) 6,600
MucorracemosusB 35,200 TobaccoMasaic 440,000
Oosporalactis 11,000 Influenze 3,400(d)
Yeasts
Saccharomyces 13,200
ellipsoideus 17,600
Saccharomycescerevisiae 13,200
Brewers'yeast 6,600
Baker'syeast 8,800
Commonyeastcake 13,200
Bacteria
Streptococcuslactis 8,800
Strep.hermolyticus(alphatype) 5,500
Staphylococcusaureus 6,600
Staphylococcusalbus 5,720
Micrococcussphaeroides 15,400
Sarcinalutea 26,400
Pseudomonasfluorescens 7,040
Escherichiacoli 7,040
Proteusvulgaris 7,480
Serratiamarcescens 6,160
Bacillussubtilis 11,000
Bacillussubtilisspores 22,000
Spirillumrubrum 6,160
References:
(a) Luckiesh,Matthew(1946)ApplicationofGermicidal,EthyemalandInfraredEnergy,D.VanNostrando.,NewYork,NewYork,pp253
(b) Hollaender(1942)Aerobiology,A.A.A.S.(for90%inactivation),pp162
(c) Ellis,C.andWells,O.O.(1941)TheChemicalActionofUltravioletRays,ReinholdPublishingCorp.,pp.713714
(d) Hollaender,A.,Oliphant,J.W.(1944)Theinactivationeffectofmonochromaticultraviolet.RadiationonInfluenzeVirus(for90%inactivation)
Jour.ofBact.48,pp.447454
(e) Thistable,"EnergiesRequiredtoDestroySomeMicroorganismsbyUltravioletRadiations"comesfromWestinghousebrochureentitled"Westinghouse
SterilampGermicidalUltravioletTubes"
OM0228 Page90of99
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11.0 Electrical/EnvironmentalRequirements
11.1 Electrical(Supplyvoltagefluctuationsnotoexceed+/10%)
*NU430400 115VAC, 60Hz, 1Phase, 8Amps
*NU430600 115VAC, 60Hz, 1Phase, 10Amps
*UL/ULCListed
11.2 OperationalPerformance(forindooruseonly)
EnvironmentTemperatureRange: 60F85F(15C30C)
EnvironmentHumidity: 20%60%RelativeHumidity
EnvironmentAltitude: (2000meters)maximum
11.3 LightExposure
StandardFluorescentLighting@150ft.candles(1614LUX)maximumintensity.
11.4 InstallationCategory: 2.0
Installationcategory(overvoltagecategory)definestheleveloftransientovervoltage,whichtheinstrumentisdesigned
towithstandsafely.Itdependsonthenatureoftheelectricitysupplyanditsovervoltageprotectionmeans.For
example,inCATII,whichisthecategoryusedforinstrumentsininstallationssuppliedfromasupplycomparableto
publicmainssuchashospitalandresearchlaboratoriesandmostindustriallaboratories,theexpectedtransientover
voltageis2500Vfora230Vsupplyand1500Vfora120Vsupply.
11.5 PollutionDegree:2.0
Pollutiondegreedescribestheamountofconductivepollutionpresentintheoperatingenvironment.Pollutiondegree2
assumesthatnormallyonlynonconductivepollutionsuchasdustoccurswiththeexceptionofoccasionalconductivity
causedbycondensation.
11.6 ChemicalExposure
Chemicalexposureshouldbelimitedtoantibacterialmaterialsusedforcleaninganddisinfecting.
CHLORINATEDANDHALOGENMATERIALSARENOTRECOMMENDEDFORUSEONSTAINLESSSTEELSURFACES.
Chamberdecontaminationcanbeaccomplishedbyparaformaldehyde,vaporphasedHydrogenPeroxideorEthylene
Oxidewithoutdegradationofcabinetmaterials.
11.7 EMCPerformance(classifiedforlightindustrial)
Emissions: EN61326
Immunity: EN61326
ClassAequipmentisintendedforuseinanindustrialenvironment.Inthe
documentationfortheuser,astatementshallbeincludeddrawingattentiontothefact
! WARNING thattheremaybepotentialdifficultiesinensuringelectromagneticcompatibilityin
otherenvironments,duetoconductedaswellasradiateddisturbances.
OM0228 Page91of99
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12.0 DisposalandRecycle
Cabinetsthatarenolongerinuseandarereadyfordisposalcontainreusablematerials.ALLcomponentswiththe
exceptionoftheHEPAfiltersmaybedisposedand/orrecycledaftertheyareknowntobeproperlydisinfected.
NOTE:
Followalllocal,stateandfederalguidelinesfordisposalofHEPAfiltersolidwaste.
BIOHAZARD
Priortoanydisassemblyfordisposal,thecabinetmustbedecontaminated.
! CAUTION
RECYCLE LEAD FREE
Component Material
BaseCabinet StainlessSteel
FrontGrill StainlessSteel
Worksurface StainlessSteel
WindowFaring StainlessSteel
WindowGlides HDPE
Window SafetyGlass
WindowFrame StainlessSteel
FrontServicePanel PaintedSteel
FrontDecorativePanel PaintedSteel
ControlCenter PaintedSteel
SupplyDiffuser Aluminum
ExhaustFilter Aluminum
HEPAFilterFrames PaintedSteel
HepexBag PVC
BlowerWheel&Housing Steel
Motor VariousSteel/Copper
PrintedWiringAssembly LeadFreeElectronic
Wire PVCCoatedCopper
Ballasts VariousSteel,Electronic
Armrest PVC
Connectors Nylon
Hardware StainlessSteelandSteel
Note:Materialtypecanbeverifiedwithuseofamagnetwithstainlessandaluminumbeingnonmagnetic.
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