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NOIDA INTERNATIONAL UNIVERSITY

PROGRAMME STRUCTURE
B.Tech BIOTECHNOLOGY (2016-17)
FIRST SEMESTER

S. No Course Subject Period Evaluation Scheme Total


Code Credits
Sessional Exam External Subject
Exam Total
L T P CA TA Total
Elementary
1 ABT 101 3 1 0 20 20 40 60 100 4
Mathematics-1
ASH 102/ Engg, Physics/
2 3 1 0 20 20 40 60 100 4
ASH 103 Engg. Chemistry
Industrial
3 ASH 104 2 0 0 20 20 40 60 100 2
Communication-I
Basic Electrical
EEL105 / Engineering/
4 3 1 0 20 20 40 60 100 4
EME 106 Mechanical
Engineering
Fundamental of
Computer
ECS 107 / Programming/
5 3 1 0 20 20 40 60 100 4
EEC 108 Fundamental of
Electronics
Engineering
Environmental
6 EBT 109 1 0 0 20 20 40 60 100 1
Studies
PRACTICALS
ASH122 /
1 Physics/Chemistry 0 0 2 - - 40 60 100 1
ASH 123
Basic Electrical
EEL125/ Engineering /
2 0 0 2 - - 40 60 100 1
EME 126 Mechanical
Engineering
Fundamental of
Computer &
ECP127 /
3 Programming / 0 0 2 - - 40 60 100 1
EEC 128
Fundamental of
Electronics
EWS 110 / Workshop Practice /
4 0 0 2 - - 40 60 100 1
EED 111 Engineering Drawing

Total 1000 23

1
SECOND SEMESTER

S. No Course Subject Period Evaluation Scheme Total


Code Credits
Sessional Exam External Subject
Exam Total
L T P CA TA Total
Elementary
1 ABT 201 3 1 0 20 20 40 60 100 4
Mathematics-1I
ASH202/ Engg, Physics/
2 3 1 0 20 20 40 60 100 4
ASH 203 Engg. Chemistry

Industrial
3 ASH 204 2 0 0 20 20 40 60 100 2
Communication-II
Basic Electrical
EEL205/ Engineering/
4 3 1 0 20 20 40 60 100 4
EME206 Mechanical
Engineering
Fundamental of
Computer &
ECS207/ Programming/
5 3 1 0 20 20 40 60 100 4
EEC208 Fundamental of
Electronics
Engineering
Environmental
6 EBT 209 1 0 0 20 20 40 60 100 1
Studies
PRACTICALS

ASH 222/ Physics/


1 0 0 2 - - 40 60 100 1
ASH 223 Chemistry

Basic Electrical
EEL225/ Engineering /
2 0 0 2 - - 40 60 100 1
EME 226 Mechanical
Engineering
Fundamental of
Computer and
3 FCP227 / Programming / 0 0 2 - - 40 60 100 1
EEC 228 Fundamental of
Electronics
Engineering
Workshop Practice /
EWS 210 /
4 Engineering 0 0 2 - - 40 60 100 1
EED 211
Drawing

Total 1000 23

2
THIRD SEMESTER

Total
Period Evaluation Scheme
Credits
Course
S. No Subject
Code
External Subject
Sessional Exam
Exam Total

L T P CA TA Total

1 EBT 301 Cell Biology 3 1 0 20 20 40 60 100 4

2 EBT 302 Biochemisty-1 3 1 0 20 20 40 60 100 4

3 EBT 303 Microbiology 3 0 0 20 20 40 60 100 4

Basic Bio analytical


4 EBT 304 3 1 0 20 20 40 60 100 4
Techniques

Computer Based
Numerical and
5 EBT 305 3 1 0 20 20 40 60 100 4
Statistical
Techniques

Industrial
6 ASH304 2 0 0 20 20 40 60 100 2
Communication-III

PRACTICALS

1 EBT 321 Cell Biology Lab 0 0 2 - - 40 60 100 1

2 EBT 322 Bio chemistry Lab 0 0 2 - - 40 60 100 1

3 EBT 323 Microbiology Lab 0 0 2 - - 40 60 100 1

Bio analytical
4 EBT 324 0 0 2 - - 40 60 100 1
Techniques Lab
Total 1000 26

3
FOURTH SEMESTER

S. No Course Subject Period Evaluation Scheme Total Credits


Code

Sessional Exam End Subject


Sem Total
Exams
C
L T P TA Total
A
THEORY
EBT
1 Food Biotechnology
401 3 1 - 20 20 40 60 100 4
-
EBT 3 1 20 20 40 60 100 4
2 Genetics
402

EBT Cell & Tissue Culture - 4


3
403 & Techniques 3 1 20 20 40 60 100
- -
EBT Data Structure & 3 20 20 40 60 100 4
4
404 Algorithms

EBT Bioprocess Engineering 3 1 - 20 20 40 60 100 2


5
405

Industrial 2 - - 20 20 40 60 100 2
5 ASH communication-IV
404
PRACTICALS

- - 2 - - 40 60 100 1
Food biotechnology
1 EBT 421
Lab

- - 2 - - 40 60 100 1
2 EBT 423 Cell & Tissue Culture
Techniques Lab

Total 800 22

4
FIFTH SEMESTER

S. No Course Subject Period Evaluation Scheme Total


Code Credits

Sessional Exam External Subject


Exam Total

L T P CA TA Total

Bioethics, Biosafety
1 EBT 501 3 1 0 20 20 40 60 100 4
& IPR

Enzymology &
2 EBT 502 Enzyme 3 1 0 20 20 40 60 100 4
Technology

Immunology &
3 EBT 503 3 1 0 20 20 40 60 100 4
Immuno technology

Bioprocess
4 EBT 504 3 1 0 20 20 40 60 100 4
Technology

Biomedical
5 EBT 505 3 1 0 20 20 40 60 100 4
Instrumentation

Industrial
6 ASH 504 2 0 0 20 20 40 60 100 2
Communication-V

PRACTICALS

Enzymology &
1 EBT 522 Enzyme Technology 0 0 2 - - 40 60 100 1
Lab

Immunology &
2 EBT 523 Immuno technology 0 0 2 - - 40 60 100 1
Lab

Bioprocess
3 EBT 524 0 0 2 - - 40 60 100 1
Technology Lab

Total 900 25

5
SIXTH SEMESTER

S. No Course Subject Period Evaluation Scheme Total


Code Credits

Sessional Exam External Subject


Exam Total
L T P CA TA Total

Downstream
1 EBT 601 3 1 0 20 20 40 60 100 4
Processing
Database
2 EBT 602 Management 3 1 0 20 20 40 60 100 4
Systems

Genetic
3 EBT 603 3 1 0 20 20 40 60 100 4
Engineering

4 EBT 604 Molecular biology 3 1 0 20 20 40 60 100 4

Statistics for
5 EBT 605 3 1 0 20 20 40 60 100 4
Biology

Industrial
6 ASH604 2 0 0 20 20 40 60 100 2
Communication-VI

PRACTICALS

Downstream
1 EBT 621 0 0 2 - - 40 60 100 1
Processing lab

2 EBT 621 Database 0 0 2 - - 40 60 100 1


Management Lab
3 EBT 624 0 40 60 100 1
Molecular biology lab 0 2

Total 900 25

SUMMER PROJECT I (6 - 8 WEEKS)

6
SEVENTH SEMESTER

S.N o Course Subject Period Evaluation Scheme Total


Code Credits

Sessional Exam External Subject


Exam Total
L T P CA TA Total

1 EBT 701 Biotechnology in 3 1 0 20 20 40 60 100 4

Health Care

Chemical
2 EBT 702 Engineering 3 1 0 20 20 40 60 100 4

Thermodynamics of
3 EBT 703 Biological 3 0 0 20 20 40 60 100 4
Systems
Current Topics in
4 EBT 704 3 1 0 20 20 40 60 100 4
Biotechnology
Industrial
5 ASH 705 3 1 0 20 20 40 60 100 2
Communication-VII

6 EBT 705 Minor Project 200 4

Total 700 22

Departmental Elective Open Elective


EBT 704 Current Topics in Biotechnology EBT 702 Chemical Engineering

Bio-Sensor Technology Chemical Engineering

Nanobiotechnology Energy Management Systems

Current Topics in Biotechnology Human Resource Management

7
EIGHTH SEMESTER

S. No Course Subject Period Evaluation Scheme Total


Code Credits

Sessional Exam External Subject


Exam Total
L T P CA TA Total
Environmental
1 EBT 801 3 1 0 20 20 40 60 100 4
Biotechnology

Entrepreneurship
Development
3 EBT 802 Programme 3 0 0 20 20 40 60 100 4

Marketing &
4 EBT 803 Management of 3 0 0 20 20 40 60 100 4
Biotechnology

5 EBT 804 Major Project (12- 800 10


14 weeks)

Total 1100 22

Departmental Elective Open Elective


EBT 801 Environmental Biotechnology EBT 802 Entrepreneurship Development Programme

Enzyme Technology Industrial Pollution Control &


Environmental Audit
Pharmaceutical Science and Drug Designing
Urban Water Management

Environmental biotechnology

Entrepreneurship Development Programme


Bio Business, IPR and Entrepreneurship

8
ELEMENTARY MATHEMATICS-I

Course Code: ABT 101 Credit Units: 04

Unit 1:
The set theory and functions: Properties of subsets, linear and geometric functions, the binomial theories
of Integer, limits of functions (basic idea of limits of functions without analytical definitions), derivatives
of functions.

Unit 2:
Logarithm: Definition of laws of logarithm, use of logarithm table.

Unit 3:
Differentiation & Integration: General introduction.

Unit 4:
Algebra of Matrices: Definition of various types of matrices, symmetric and skew symmetric matrices,
Hermit Ian and skew Hermit Ian matrices, determinants up to order four, elementary properties of
determinants, product of determinants, inverse of a matrix, rank of matrix, linear equations.

Unit 5:
Introduction to Biostatistics: Sampling techniques, presentation of data, tabulation, frequency
distribution, graphical representation of data by Histogram, frequency polygon, frequency curve and
cumulative frequency curve.

Unit 6:
Measures of central tendency: Mean, Median & Mode.

Unit 7:
Measures of Dispersion: Mean deviation, standard deviation and standard error; variance concept,
calculation and theories of probability.

Unit 8:
Test of Significance: t- test, z-test, chi-square test and F-test.

References:
1 S.C Gupta & V.K Kapoor; Fundamentals of Statistics, Vol.1
2. Goon Gupta &Das Gupta: Fundamental of Statistics, Vo1,
3. Statistical methods in Biology by Norman Bailey
4. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003
9
ENGINEERING PHYSICS

Course Code: ASH 102 Credit Units: 04


UNIT-I

Relativistic Mechanics: Inertial and Non-inertial Frames, Postulates of Special Theory of Relativity,
Lorentz Transformation, Length Contraction and Time Dilation, Addition of Velocities,
Mass Energy Equivalence and Variation of Mass with Velocity. Radiation: Kirchhoffs Law, Stefans
law (only statement), Energy spectrum of Blackbody Radiation.

UNIT-II

Interference: Coherent Sources, Conditions of Interference, Fresnels Biprism Experiment,


Displacement of Fringes, Interference in Thin Films Wedge Shaped Film, Newtons Rings. Diffraction:
Single and n-Slit Diffraction, Diffraction Grating, Raleighs Criterion of Resolution, Resolving Power of
Grating.

UNIT-III

Polarization: Phenomenon of Double Refraction, Ordinary and Extra-ordinary Rays, Nicol Prism,
Production and Analysis of Plane, Circularly and Elliptically Polarized Light, Optical Activity, Specific
Rotation, Polari meter. Laser: Principle of Laser Action, Einsteins Coefficients, Construction and
Working of He-Ne and Ruby Laser.

UNIT-IV

Electromagnetic: Amperes Law and Displacement Current, Maxwells Equations in Integral and
Differential Forms, Electromagnetic Wave Propagation in Free Space and Conducting Media, Pointing
Theorem. Phenomenon of Hysteresis and Its Applications

UNIT-V

Superconductivity:-
Essential properties of superconductors (zero resistivity), penetration depth and coherence length,
Meissner effect, critical field , Type I and Type II superconductors, applications of superconductors. Wave
Mechanics: Wave Particle Dualit y, de Broglie Concept of Matter Waves, Heisenberg Uncertainty
Principle, Schrdinger Wave Equation and Its Applications: Particle in a Box.

10
Reference Books:
1. Introduction to Special theory of Relativity Robert Resnick Wiley India
2. Physics of Atoms, Wehr Richards &Adia
3. Fundamentals of Physics, Halliday, Wiley India
4. Engineering Electromagnetics, WilliamHayt, 7t hEd,TMH
5. Ashutosh Asthana, Engg. Physics, BS Publication, Hyderabad

11
ENGINEERING CHEMISTRY

Course Code: ASH -103 Credit Units: 04

UNIT I GENERAL & ORGANIC CHEMISTRY

Molecular orbital theory, Molecular orbital diagram of diatomic molecules, hybridization, hydrogen
bonding, band theory of solids, liquid crystals with their classification applications, Braggs Law,
Fullerenes & their application , Nature of organic molecules, attacking reagents, inductive effect,
electromeric, mesomeric (resonance) effect, hyper conjugation, reaction intermediates types of
organic reaction (substitution, addition, elimination reaction &organic rearrangements), Saytzeffs
rule, organic name reactions (cannizzaros reaction, aldol condensation, Pinnacol- pinnacolon
rearrangement, Beckmanns rearrangement, Hoffmanns rearrangement), Optical isomerism &
confirmations, E-Z nomenclature, R-S configuration.

UNIT II PHYSICAL & WATER CHEMISTRY

Rate of reaction, order & molecularity of reaction, Zero order, First Order, steady state
approximation, concept of activation energy, energy barrier, cell potential, liquid junction potential,
conductance & its variation with dilution, Transport no. Kohlraushs Law and its application, pH,
buffer solution, calculation of pH of buffer mixture solubility & solubility Product, Nernst distribution
law & its application, corrosion, its type, Mechanism & control , Theory of Electrochemical
corrosion. Hardness of water, boiler feed water, Softening of water (Calgon Process, Zeolite process,
Lime Soda process & Ion exchange process), Reverse osmosis, treatment of boiler feed water.

UNIT III CHEMISTRY OF ENGINEERING MATERIALS

Introduction & classification of polymers, Types of Polymerization, bulk solution, suspension &
emulsion, copolymers, vulcanization, PVC, Polyamides, Polyurethane, Polyethylene, Poly propylene,
PET, Resins (Phenol Formaldehyde), PMMA, PAN, Rubber, Conducting and Biodegradable polymers,
Pyroceramics, Toughened glass, Strengthening of glass, Refractories, Nano Composites, Protective
Coatings, Fe, Al, Cu, Pb& Zn alloys, Organometallics & their applications.

UNIT IV FUELS & COMBUSTION

Classification of Fuels, calorific value of fuel, gross & net calorific value, determination of calorific value
using Bomb calorimeter, Coal, Biomass and Biogas, Introduction of Lubricants, Mechanism of
Lubrication, Classification of Lubricant, Bio Lubricant, Flash and Fire Point, Pour Point, Cloud Point,
Aniline point, Viscosity index.

12
UNIT V ANALYTICAL METHODS AND APPLICATIONS

Titrimetric analysis with reference to acid-base, redox, precipitation and complex ometrictitrations.
Elementary ideas and simple applications of UV, visible, mass and NMR spectral techniques H NMR
spectral techniques.

REFERENCE BOOKS
1. Engineering Chemistry Wiley India
2. Engineering chemistry by Sivasankar, TMH, New Delhi.
3. Organic Chemistry by Morrisson& Boyd, Pearson Publication.
4. Organic Chemistry by Loudon, Oxford University Press.
5. C Parameswara Murthy, C V Agrawal and etal., Engineering Chemistry, BS
Publication, Hyderabad

13
INDUSTRIAL COMMUNICATION-I

Course Code: ASH 104 Credit Units: 02

Objective: The objective of the first semester course is to introduce the basics of communication language
and gradually develop a module to exhilarate the command over the language and its acquisition.

Unit -1: Introduction to professional Communication


Definition of Communication
Types of communication
Channels of communication
Barriers to communication
General and Technical Communication

Unit-2: English language on global forum


Language as a tool of communication
English language in global business environment
Cultural Variables

Unit 3: Speaking abilities enhancement


Group Discussion
Role of moderators
Objectives of GD
Types of GD
Participation in GD

Unit-4: Exposure to English literature


The necklace - Guy de Maupassant (Short Story)
Mending Wall Robert Frost (Poem)
Ode on a Grecian urn- John Keats(Poem)

14
BASIC ELECTRICAL ENGINEERING

Course Code: EEL 105 Credit Units: 04


UNIT-1

D.C. Network Theory: Circuit theory concepts-Mesh and node analysis. Network Theorems- Super-
position theorem. Thevenins theorem, Nortons theorem, Maximum, Power Transfer theorem,
Star Delta transformation.

Unit-2
Steady State Analysis of A.C. Circuits: Sinusoidal and phase or representation of voltage and current:
single phase A.C. circuit behavior of resistance, inductance and capacitance and their combination in
series & parallel and power factor, series parallel resonance-band width and quality factor
UNIT-3

Three Phase A.C. Circuits : Star-Delta connections, line and phase voltage/current relations, three
phase power and its measurement.

UNIT-4

Transformer : Principle of operation, types of construction, phasor diagram, equivalent circuit,


efficiency and voltage regulation of single phase transformer, O.C. and S.C. tests.

Unit-5

Rotating Machine : Principle and construction of D.C. Machines ,Synchronous machine and induction
motor. Single phase Motors : Principle of operation and methods of starting of induction motor.Special
Machines: principle of operation and methods of starting of Stepper motor and Universal motor

Unit-6

Measuring Instruments: construction and principle of operation of voltage and current


measuring instruments; introduction to power and energy meters

References :

1. Dr. R. K. Singh and Dr. P.S. Subramanyam, Basic Electrical Engineering, BS


Publication, Hyderabad.
2.Gaikwad, Basic Electrical Engineering, Wiley India
3. A.E. Fitgerald, D.E., Higginbotham and AGrabel, Basic Electrical Engineering
McGraw Hill.
4. H. Cotton, Advanced Electrical Technology Wheeler Publishing.

15
MECHANICAL ENGINEERING

Course Code: EME 106 Credit Units: 04

Unit-1Fundamental Concepts and Definitions: Definition of thermodynamics, System, Surrounding


and universe, Phase, Concept of continuum, Macroscopic & microscopic point of view. Density, Specific
volume, Pressure, temperature. Thermodynamic equilibrium, Property, State, Path, Process, Cyclic and
non -cyclic processes, reversible and irreversible processes, Quasi static process, Energy and its forms,
Enthalpy.

UNIT-2 Law of Thermodynamics : Zeroth law: Zeroth law, Different temperature scales and
temperature measurement First law:First law of thermodynamics. Processes - flow and non-flow, Control
volume, Flow work and non-flow work, Steady flow energy equation, Unsteady flow systems and their
analysis. Second law: Limitations of first law of thermodynamics, Essence of second law, Thermal
reservoir, Heat engines. COP of heat pump and refrigerator. Statements of second law and their
equivalence, Carnot cycle, Carnot theorem, Thermodynamic temperature scale, Clausius inequality.
Concept of entropy.

UNIT-3 Properties of steam and Basic of IC engine: Properties of steam, Phase transformation process
and its graphical representation on P-V, T-V & T-s diagram, Mollier diagram and Steam Tables,
Processes involving steam in closed and open systems. Introduction to I.C. Engines: Two & four stoke
S.I. and C.I. engines. Otto cycle, Diesel cycle, Dual cycle.

UNIT-4 Force system and Analysis: Force system and Analysis Basic concept: Review of laws of
motion, transfer of force to parallel position, resultant of planer force system, Free Body Diagrams,
Equilibrium. Friction: Introduction, Laws of Coulomb friction, Equilibrium of bodies involving dry
fiction. Structure Analysis Beams: Introduction, Shear force and bending moment, Shear force and
bending moment diagram for statically determinate beams. Trusses: Introduction, Simple Trusses,
Determination of forces in simple truss members, Method of joints and Method of section.

UNIT-5 Stress and Strain Analysis : Simple stress and strain: Introduction, Normal shear stresses,
Stress-strain diagrams for ductile and brittle materials, Elastic constants, One dimensional loading
of members of varying cross section, Strain energy, Thermal stresses. Compound stress and strains:
Introduction, State of plane stress, Principal stress and strain, Mohrs circle for stress and strain. Pure
Bending of Beams: Introduction, Simple bending theory, Stress in beams of different cross sections. Torsion:
Introduction, Torsion of Shafts of circular section, Torque and Twist, Shear stress due to Torque.

Reference:
1. Agarwal, Basic Mechanical Engineering, Wiley India
2. Holman, J.P.: Thermodynamics, McGraw Hill book Co. NY.
3. Singh Onkar, Bhavikatti S.S., Chandra Suresh: Introduction to Mechanical Engineering:
Thermodynamics, Mechanics and Strength of Materials, New Age International Publishers
4. Yadav R.: Thermodynamics and Heat Engines, Vol I & II (SI Edition) Central Publ. House.
16
FUNDAMENTALS OF COMPUTER & PROGRAMMING

Course Code: ECS 107 Credit Units: 04

Unit 1
Basics of Computer System

Block Diagram of a Computer, Booting Process, Types of Computer Systems, Introduction to the
concepts: Bit, Byte, Word, hardware, Operating System and Application Software, Compilers,
Assemblers, Loaders and Linkers. ASCII& EBCDIC codes, Binary, Octal, Decimal & Hexadecimal
Number Systems & their Conversions

Unit 2

Introduction to Computer Operating System

Features of DOS, Windows and UNIX Operating System and their Comparison, Concept of flowchart and
Algorithm, Machine, Assembly and high level languages, Structured Programming, Use of high level
programming languages for the systematic development of programs.

Unit 3

C Fundamentals

Character Set, Identifiers & Keywords, Data Types, Constants, Variables, Tokens, Operators &
Expression, Library Functions, Statements, Symbolic Constants, Preprocessor Directives, Functions.

Unit 4

Arrays and Structure

Introduction, Classification of Array: One Dimensional Array, Multidimensional Array, Two Dimensional
Arrays. Defining a Structure, Array vs. Structure, Initialization of a Structure, Nested Structure

Unit 5
Pointers and File handling
Pointers Variables, Pointer Operator, Pointer Expression, Array of Pointers , Multiple Indirection,
Pointers to functions, dynamic allocation functions, File I/O: Streams and files, file System basic, fread,
fwrite ,fseeek, random access I/O, fprintf(), fscanf(), standard streams.

Reference Book
1. Let Us C: Yashwant Kanetkar [BPB]
2. Jones, C Programming with problem solving, Wiley India
3. Gupta: Computer Concepts & C Programming, Comdex
17
FUNDAMENTALS OF ELECTRONIC ENGINEERING

Course Code: EEC 108 Credit Units: 04

Unit 1: Semiconductor materials and properties :Group-IV materials, Covalent bond, electron-hole
concepts, Basic concepts of energy bands in materials, concepts of forbidden gap, Intrinsic and extrinsic
semiconductors, donors and acceptors impurities, Junction diodep-n junction, depletion layer, v- i
characteristics, dioderesistance, capacitance diode ratings ( average current, repetitive peak current,
non-repetitive current, peak-inverse voltage).
Unit-2: Diode Applications: Rectifiers (half wave and full wave), calculation of transformer utilization
factor and diode ratings, Introduction to filters, calculation of ripple factor and load regulation, clipping
circuits, clamping circuits, voltage multipliers Breakdowndiodes breakdown mechanisms (zener and
avalanche), breakdown characteristics, zener resistance, zener diode ratings, zener diode application as
shunt regulator

UNIT-3: Bipolar Junction Transistor : Basic construction, transistor action, CB, CE and CC
configurations, input/output Characteristics, concept of Biasing of transistors-fixed bias, emitter bias,
potential divider bias, Transistor Amplifier, Graphical analysis of CE amplifier, concept of voltage gain,
current gain, h-parameter model (low frequency), computation of Ai, Av, Ri, R O of single transistor CE
and CC amplifier configurations.

Unit-4: Field Effect Transistor : FET: Basic construction, transistor action, concept of pinch off,
maximum drain saturation current, input and transfer characteristics, characteristics equation CG, CS and
CD configurations, Introduction to self and fixed biasing .MOSFFT: depletion and enhancement type
MOSFET-construction, operation and characteristics. Computation of Av, Ri, Ro, of single FET
amplifiers using all the three configurations

Unit-5: Switching theory and logic design : Number systems, conversions of bases, Boolean algebra,
logic gates, concept of universal gate, concept of K- Map Operational Amplifiers: Concept of ideal
operational amplifiers, ideal op-amp parameters, inverting, non-inverting and unity gain amplifiers.

Reference Books:

1. RobertBoylestad, Electronic Devices and Circuit theory, Pearsons Educations.


2. R. K. Singh &LalKishor, Basic Electronics Engineering, B S Publication, Hyderabad.
3. Gaikwad, Basic Electronics, Wiley India
4. A Mottershead, Electronic devices and circuits. PHI, 2000.
5. Morris Mano, Digital Computer Design, PHI, 2003.

18
ENVIRONMENTAL STUDIES

Course Code: EBT 109 Credit Units: 02

UNIT 1

NATURAL RESOURCES:
Renewable and Non-renewable Resources : Natural resources and associated problems.

a) Forest resources: Use and over-exploitation, deforestation, case studies. Timber extraction,
mining, dams and their effects on forest and tribal people.

b) Water resources: Use and over-utilization of surface and ground water, floods, drought, conflicts
over water, dams-benefits and problems.

c) Mineral resources: Use and exploitation, environmental effects of extracting and using mineral
resources, case studies.

d) Food resources : World food problems, changes caused by agriculture and over-grazing, effects of
modern agriculture, fertilizer-pesticide problems, water logging, salinit y, case studies.

e) Energy resources : Growing energy needs, renewable and non- renewable energy sources, use of
alternate energy sources. Case studies.

f) Land resources : Land as a resource, land degradation, man induced landslides, soil erosion and
desertification.
Role of an individual in conservation of natural resources.
Equitable use of resources for sustainable lifestyles.

UNIT 2
ECOSYSTEMS
Concept of an ecosystem.
Structure and function of an ecosystem.
Producers, consumers and decomposers.
Energy flow in the ecosystem.
19
Ecological succession.
Food chains, food webs and ecological pyramids.

Introduction, types, characteristic features, structure and function of the following


Ecosystems:-
a. Forest ecosystem

b. Grassland ecosystem
c. Desert ecosystem

d. Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries)

UNIT 3
BIODIVERSITY AND ITS CONSERVATION
Introduction Definition: genetic, species and ecosystem diversity.
Biogeographically classification of India
Value of biodiversity: consumptive use, productive use, social, ethical, aesthetic and option values
Biodiversity at global, National and local levels.
India as a mega-diversity nation
Hot-sports of biodiversity.
Threats to biodiversity : habitat loss, poaching of wildlife, man-wildlife conflicts.
Endangered and endemic species of India
Conservation of biodiversity: In-situ and Ex-sit conservation of biodiversity.

UNIT 4
ENVIRONMENTAL POLLUTION & SOCIAL ISSUES
Definition

Cause, effects and control measures of:-


a. Air pollution
b. Water pollution
20
c. Soil pollution
d. Marine pollution
e. Noise pollution
f. Thermal pollution
g. Nuclear hazards
h Disaster management : floods, earthquake, cyclone and landslides.
i. From Unsustainable to Sustainable development
j. Urban problems related to energy
k. Water conservation, rain water harvesting, watershed management
l. Environmental ethics: Issues and possible solutions.

m. Climate change, global warming, acid rain, ozone layer depletion, nuclear accidents and holocaust.
Case studies.
n. Wasteland reclamation.
o. Consumerism and waste products.

REFERENCE BOOK
1. MAjni Reddy, Text book of environmental Science, BS Publication, Hyderabad
2. Environmental Studies by Daniel, Wiley India
3. Environmental Studies by ErachBharucha, University Press.
4. Fundamental of Ecology, E.P.Odum, Cengage Learning.
5. Environmental Science and Engineering by Wright, Pearson Publication.6.
6. First Ecology by Beeby and Brennan, Oxford University Press.
7. Environment Science by Miller, Cengage Learning, New Delhi.

8. Hand book of Environmental laws, Rules, Guidelines, Compliances and Standards Vol. 1 & Vol. 2,
Bharat Publication, New Delhi.

21
PHYSICS PRACTICALS

Course Code: ASH 122 Credit Units: 01

List of Experiments (At least Ten)

1. To determine the wavelength of monochromatic light by Newtons ring.

2. To determine the wavelength of monochromatic light with the help of Fresnel s biprism.

3. To determine the focal length of two lenses by nodal slide and locate the position of cardinal points.

4. To determine the specific rotation of cane sugar solution using half shade polarimeter.

5. To determine the wavelength of spectral lines using plane transmission grating.

6. To determine the specific resistance of the material of given wire using Carey Fosters bridge.

7. To determine the variation of magnetic field along the axis of a current carrying coil and then to
estimate the radius of the coil.

8. To verify Stefans Law by electrical method.

9. To calibrate the given ammeter and voltmeter.

10. To study the Hall Effect and determine Hall coefficient, carnier density and mobility of a given
semiconductor material using Hall-effect set up.

11. To determine energy bank gap of a given semiconductor material.

12. To determine E.C.E. of copper using Tangent or Helmholtz galvanometer.

13. To draw hysteresis curve of a given sample of ferromagnetic material and from this to determine
magnetic susceptibility and permeability of the given specimen.

14. To determine the balistic constant of a ballistic galvanometer.

15. To determine the viscosity of a liquid.

Note: Additional experiments may be added based on contents of syllabus.

22
CHEMISTRY LAB

Course Code: ASH 123 Credit Units: 01

LIST OF EXPERIMENTS (AT LEAST TEN)

1. Determination of alkalinity in the given water sample.

2. Determination of temporary and permanent hardness in water sample using EDTA as standard solution.

3. Determination of available chlorine in bleaching powder.

4. Determination of chloride content in the given water sample by Mohrs method.

5. Determination of iron content in the given ore by using external indictor

6. pH-metric titration.

7. Determination of Equivalent weight of Iron by the chemical displacement method. The equivalent
weight of copper is 63.5

(Note: the procedure to be followed in carrying the above experiment is given as annexure)

8. Viscosity of an addition polymer like polyester by Viscometer.

9. Determination of iron concentration in sample of water by colorimetric method. The method involves
the use of KCNS as color developing agent and the measurements are carried out at l max 480 nm.

Note: The general procedure of estimation is given on pp653-8 of the textbook of Quantitative Chemical
Analysis by A.I. Vogel 6th Edition, Publisher: Pearson education Ltd. 2000)

10. Determination of heat of neutralization of hydrochloric acid and Sodium hydroxide

11. Determination of amount of dissolved oxygen in water

12. Separation of metal ions by paper chromatography

13. Preparation of iodoform from acetone

14. Preparation of Sodium Cobalt nitrile salt.

15. Element detection & functional group identification in organic compounds

23
BASIC ELECTRICAL LAB

Course Code: EEL 125 Credit Units: 01

Fundamentals of electrical Lab (Any Ten)

1. Verification of Kirchhoffs Law (KVL & KCL).

2. Study of phenomenon n of resonance in RLC series circuit.

3. Verification of superposition theorem

4. Verification of Thevenins theorem.

5. Verification of Nortons theorem.

6. Verification of maximum power transfer theorem.

7. Measurement of power in a three phase circuit by two wattmeter method.

8. Measurement of efficiency of a single phase transformer by load test.

9. Determination of parameters and losses in a single phase transformer by OC and SC test.

10. Study running and reversing of a three phase induction motor.

11. Study of a single phase energy meter.

24
MECHANICAL ENGINEERING LAB

Course Code: EME-126 Credit Units: 01

List of experiments (Any ten)

1. Study of Steam engine and steam turbine models.

2. Study of 2-stroke and 4 -stroke I.C Engine models.

3. Study of a vapor compression Refrigeration unit tutor/refrigerator.

4. Study of a window type air conditioner.

5. To conduct the tensile test on a UTM and determine ultimate Tensile strength, percentage elongation
for a steel specimen.

6. To conduct the compression test and determine the ultimate compressive strength for a specimen.

7. To conduct the Impact test (Izod / charpy) on the Impact testing machine and to find the impact
strength.

8. To determine the value of acceleration due to gravity by Atwoods Machine apparatus.

9. To verify the principle of moment by Bell Crank Lever Apparatus

10. To determine the moment of inertia of a flywheel apparatus about its axis of rotation

11. To verify Newtons second law of motion by Fletchers Trolley apparatus

12. To find out coefficient of friction by combined inclined plane & friction slide apparatus

13. To determine the velocity ratio, mechanical advantage & efficiency of a single purchase crab
apparatus & draw graph between load vs effort, mechanical advantage and efficiency.

14. To determine the velocity ratio, mechanical advantage & efficiency of a double purchase crab
apparatus.

25
FUNDAMENTALS OF COMPUTER PROGRAMMING LAB

Course Code: ECP-1 27 Credit Units: 01

List of Experiments

1. Practice of some internal and External DOS Commands

2. WAP to determine whether given number is even or odd

3. File and program management in windows

4. WAP to find hexadecimal and octal value of a number

5. WAP to swap two numbers

6. WAP to find sum of all the digits of a number

7. TO simulate calculator the perform the following operations on two numbers: Addition, subtraction,
Multiplication, division.

8. WAP to read two matrixes and multiply them

9. WAP to make a copy of a text file using EOF,FGETC and FPUTE

10. WAP to generate table of any number using pointer

11. WAP to find weather given number is Pallondroning or not.

12. WAP to calculate the simple interest for a set of values representing principle, number of years and
rate of interest.

Note: List may be modified according to new software available

26
FUNDAMENTALS OF BASIC ELECTRONIC ENGINEERING LAB

Course Code: EEC-128 Credit Units: 01

List of Experiments (Any Ten)

1. Study of diode characteristics.

2. To study a half wave and full wave rectifier circuit.

3. Determination of ripple factor of capacitive and non -capacitive filter for HW and FW diode rectifier
circuit.

4. Study of characteristics of Zener Diode as constant voltage.

5. Verification of Application of Zener Diode as shunt regulator.

6. Study of Clipper and Clamper Circuit with different waveforms.

7. Determination of characteristics of BJT in CB and CE configuration.

8. Determination of characteristics of FET in CS and CD configuration.

9. Study of BJT as single stage amplifier and determination of Ai, Vi, Ri and Ro.

10. Study of AND, NAND, OR, NOR and EXOR gates.

11. Verification of Universal gates.

27
WORKSHOP PRACTICE

Course Code: EWS 110 Credit Units: 01

1. Carpentry Shop: 1. Study of tools and operation and carpentry joints. 2. Simple exercise using jack
plain. 3. To prepare half- lap corner joint, mortise and tennon joints. 4. Simple exercise on woodworking
lathe.

2. Fitting Bench Working Shop: 1. Study of tools and operations 2. Simple exercises involving filling
work. 3. Making perfect male-female joint 4. Simple exercise involving drilling/tapping/dieing.

3. Black Smithy Shop: 1. Study of tools and operations. 2. Simple exercises based on black smithy
operations such as upsetting, drawing down, punching, bending, fullering& swaging.

4. Welding Shop: 1. Study of tools and operations . 2. Simple butt joint. 3. Lap joint. 4. oxy acetylene
welding.

5. Sheet metal shop: 1.Study of tools and operations. 2. Making funnel complete with soldering. 3.
Fabrication of tool box, tray, electrical panel box etc

6. Machine Shop: 1. Study of tools and operations. 2. Plane turning. 3. Step turning. 4. Taper turning 5.
Threading. 6. Single point cutting tool grinding.

Reference:

1. Hajra, Bose, Roy: Workshop Technology Vol 1 & 2, Media Promotors


2. Raghuvanshi B.S.: Workshop Technology, Vol 1 & 2, Dhanpatrai

28
ENGINEERING DRAWING

Course Code: EED 111 Credit Units: 01

1. Graphics as a tool to communicate ideas, Lettering and dimensioning, Construction of geometrical


figures like pentagon and hexagon.

2. Orthographic Projection

Principles of orthographic projections, Principal and auxiliary planes, First and Third angle projections.
Projection of points. Pictorial view. Projection of lines parallel to both the planes.
Parallel to one and inclined to other, Inclined to both the planes. Application to practical problems.
Projection of solid in simple position, Axis or slant edge inclined to one and parallel to other plane, Solids
lying on a face or generator on a plane.Sectioning of solids lying in various positions, True shape of the
section. Development of lateral surfaces, sheet metal drawing.

3. Isometric Projection Principles of isometric projection, Isometric projection using box and
offset methods.

Reference books:

1. Bhatt. N.D.: Elementary Engineering Drawing, Charothar Publishing.

2. D A Hindoliya, Text book of Egg. Graphics, BS Publication, Hyderabad

29
ELEMENTARY MATHEMATICS-II

Course Code: ABT-201 Credit Units: 04

Unit-1
Probability Theory, Random Sampling, Binomial, Poisson, Normal Distributions. : mean, variance of
a distribution and probability of variables following these distributions.
Unit-II
Skewness, kurtosis of a distribution, moment generating function, Correlation: based on Measures of
central tendencies, Karl Pearson, Rank correlation , Regression: regression coefficient, & their properties.
Unit -III
Testing of Hypothesis: Concepts of Sampling, Statistics& Parameter, Types of errors, Level of
Significance, power of test, Critical region, Analysis of Variance (ANOVA):CRD, RBD designs, Problem
oriented approach to illustrate application of above Statistical methods

Unit-IV
Formulation of differential equations, solution of Ordinary differential Equations of 1st order , exact
differential equations of 1st order with constant coefficients: Complementary function and particular
integrals Solution of linear Partial Differential equations with constant coefficients & their classifications
into parabolic, elliptic and hyperbolic with illustrative examples.
References:
1. W. Danial : Biostatistics : A foundation for Analysis in Health Sciences, John Wiley and Sons
Inc; 2004.
2. A. Edmondson and D. Druce : Advanced Biology Statistics, Oxford University Press; 1996
3. Biostatistics:: A foundation for analysis in Health Science. 7 th Edition Wayne Daniel
4. Statistical methods in Biology by Norman Bailey
5. H. S. Bear: Understanding Calculus, John Wiley and Sons (Second Edition); 2003

30
ENGINEERING PHYSICS

Course Code: ASH 202 Credit Units: 04


UNIT-I

Relativistic Mechanics: Inertial and Non-inertial Frames, Postulates of Special Theory of Relativity,
Lorentz Transformation, Length Contraction and Time Dilation, Addition of Velocities,
Mass Energy Equivalence and Variation of Mass with Velocity. Radiation: Kirchhoffs Law, Stefans
law (only statement), Energy spectrum of Blackbody Radiation.

UNIT-II

Interference: Coherent Sources, Conditions of Interference, Fresnels Biprism Experiment,


Displacement of Fringes, Interference in Thin Films Wedge Shaped Film, Newtons Rings.
Diffraction: Single and n-Slit Diffraction, Diffraction Grating, Raleighs Criterion of Resolution,
Resolving Power of Grating.

UNIT-III

Polarization: Phenomenon of Double Refraction, Ordinary and Extra-ordinary Rays, Nicol Prism,
Production and Analysis of Plane, Circularly and Elliptically Polarized Light, Optical Activity, Specific
Rotation, Polari meter. Laser: Principle of Laser Action, Einsteins Coefficients, Construction and
Working of He-Ne and Ruby Laser.

UNIT-IV

Electromagnetic: Amperes Law and Displacement Current, Maxwells Equations in Integral and
Differential Forms, Electromagnetic Wave Propagation in Free Space and Conducting Media, Pointing
Theorem. Phenomenon of Hysteresis and Its Applications

UNIT-V

Superconductivity:- Essential properties of superconductors (zero resistivity), penetration depth and


coherence length, Meissner effect, critical field , Type I and Type II superconductors, applications of
superconductors. Wave Mechanics : Wave Particle Dualit y, de Broglie Concept of Matter Waves,
Heisenberg Uncertainty Principle, Schrdinger Wave Equation and Its Applications: Particle in a Box.

Reference Books:
1. Introduction to Special theory of Relativity Robert Resnick Wiley India
2. Physics of Atoms, Wehr Richards &Adia
3. Fundamentals of Physics, Halliday, Wiley India
4. Engineering Electromagnetics, WilliamHayt, 7t h
5. Ed.(TMH)
6. AshutoshAsthana, Engg. Physics, BS Publication, Hyderabad

31
ENGINEERING CHEMISTRY

Course Code: ASH -203 Credit Units: 04

UNIT I GENERAL & ORGANIC CHEMISTRY

Molecular orbital theory, Molecular orbital diagram of diatomic molecules, hybridization, hydrogen
bonding,band theory of solids, liquid crystals with their classification applications, Braggs Law,
Fullerenes & their application , Nature of organic molecules, attacking reagents, inductive effect,
electromeric, mesomeric (resonance) effect, hyper conjugation, reaction intermediates types of
organic reaction (substitution, addition, elimination reaction &organic rearrangements), Saytzeffs
rule, organic name reactions (cannizzaros reaction, aldol condensation, Pinnacol-pinnacolon
rearrangement, Beckmanns rearrangement, Hoffmanns rearrangement), Optical isomerism &
confirmations, E-Z nomenclature, R-S configuration.

UNIT II PHYSICAL & WATER CHEMISTRY

Rate of reaction, order &molecularity of reaction, Zero order, First Order, steady state approximation,
concept of activation energy, energy barrier, cell potential, liquid junction potential, conductance &
its variation with dilution, Transport no. Kohlraushs Law and its application, pH, buffer solution,
calculation of pH of buffer mixture solubilit y & solubility Product, Nernst distribution law & its
application, corrosion, its type, Mechanism & control , Theory of Electrochemical corrosion.
Hardness of water, boiler feed water, Softening of water (Calgon Process, Zeolite process, Lime Soda
process & Ion exchange process), Reverse osmosis, treatment of boiler feed water.

UNIT III CHEMISTRY OF ENGINEERING MATERIALS

Introduction & classification of polymers, Types of Polymerization, bulk solution, suspension &
emulsion, copolymers, vulcanization, PVC, Polyamides, Polyurethane, Polyethylene, Poly propylene,
PET, Resins (Phenol Formaldehyde), PMMA, PAN, Rubber, Conducting and Biodegradable polymers,
Pyroceramics, Toughened glass, Strengthening of glass, Refractories, Nano Composites, Protective
Coatings, Fe, Al, Cu, Pb& Zn alloys, Organometallics & their applications.

UNIT IV FUELS & COMBUSTION

Classification of Fuels, calorific value of fuel, gross & net calorific value, determination of calorific value
using Bomb calorimeter, Coal, Biomass and Biogas, Introduction of Lubricants, Mechanism of
Lubrication, Classification of Lubricant, Bio Lubricant, Flash and Fire Point, Pour Point, Cloud Point,
Aniline point, Viscosit y index.

32
UNIT V ANALYTICAL METHODS AND APPLICATIONS

Titrimetric analysis with reference to acid-base, redox, precipitation and complex metrictitrations.
Elementary ideas and simple applications of UV, visible, mass and NMR spectral techniques H NMR
spectral techniques.

REFERENCE BOOKS
1. Engineering Chemistry Wiley India
2. Engineering chemistry by Sivasankar, TMH, New Delhi.
3. Organic Chemistry by Morrisson& Boyd, Pearson Publication.
4. Organic Chemistry by Loudon, Oxford University Press.
5. C Parameswara Murthy, C V Agrawal and etal., Engineering Chemistry, BS
Publication, Hyderabad

33
INDUSTRIAL COMMUNICATION-II

Course Code : ASH204 Credit Units: 02

Objective: Second module functions to enhance the ability of Reading skills and develop an attitude
towards language learning and comprehension skills.

Unit-1: Learning Reading Skills


Importance and need of Reading Skills
Types of Reading Skills
Methods & objectives of Reading Skills

Unit-2: Jargon & Vocabulary building


Examples of jargon
Technical Terminology
One word Vocabulary
Prefixes, Suffixes, Homophones
Synonyms & Antonyms

Unit-3: Biographical reading


Reading biography of Mahatma Gandhi
Discourse sessions
Summary of reading
Meaning of difficult words

Unit -4: English Literature


Animal Farm George Orwell (Drama)
Tintern Abbey William Wordsworth (poem)
Of Studies Francis Bacon(Essay)

34
BASIC ELECTRICAL ENGINEERING

Course Code: EEL 205 Credit Units: 04


UNIT-1

D.C. Network Theory :Circuit theory concepts-Mesh and node analysis.Network Theorems- Super-
position theorem.Thevenins theorem, Nortons theorem, Maximum, Power Transfer theorem, Star
Delta transformation.

Unit-2

Steady State Analysis of A.C. Circuits: Sinusoidal and phase or representation of voltage and current:
single phase A.C. circuit behavior ofresistance, inductance and capacitance and their combination in series
& parallel and power factor, series parallel resonance-band width and quality factor

UNIT-3

Three Phase A.C. Circuits : Star-Delta connections, line and phase voltage/current relations, three
phase power and its measurement.

UNIT-4

Transformer : Principle of operation, types of construction, phasor diagram, equivalent circuit,


efficiency and voltage regulation of single phase transformer, O.C. and S.C. tests.

Unit-5

Rotating Machine :Principle and construction of D.C. Machines ,Synchronous machine and induction
motor.Single phase Motors : Principle of operation and methods of starting of induction motor.Special
Machines: principle of operation and methods of starting of Stepper motor and Universal motor

Unit-6

Measuring Instruments: onstruction and principle of operation of voltage and current measuring
instruments; introduction to power and energy meters

References :
1. Dr. R. K. Singh and Dr. P.S. Subramanyam, Basic Electrical Engineering, BS
Publication, Hyderabad.
2. Gaikwad, Basic Electrical Engineering, Wiley India
3. A.e. Fitgerald, D.E., Higginbotham and A Grabel, Basic Electrical Engineering McGraw Hill.
4. H. Cotton, Advanced Electrical Technology Wheeler Publishing.

35
MECHANICAL ENGINEERING

Course Code: EME 206 Credit Units: 04

Unit-1: Fundamental Concepts and Definitions : Definition of thermodynamics, System, Surrounding


and universe, Phase, Concept of continuum, Macroscopic & microscopic point of view.Density, Specific
volume, Pressure, temperature.Thermodynamic equilibrium, Property, State, Path, Process, Cyclic and
non cyclic processes, Reversible and irreversible processes, Quasi static process, Energy and its forms,
Enthalpy.

UNIT-2: Law of Thermodynamics : Zeroth law: Zeroth law, Different temperature scales and
temperature measurement First law:First law of thermodynamics. Processes - flow and non-flow, Control
volume, Flow work and non-flow work, Steady flow energy equation, Unsteady flow systems and their
analysis. Second law: Limitations of first law of thermodynamics, Essence of second law, Thermal
reservoir, Heat engines. COP of heat pump and refrigerator. Statements of second law and their
equivalence, Carnot cycle, Carnot theorem, Thermodynamic temperature scale, Clausius inequality.
Concept of entropy.

UNIT-3: Properties of steam and Basic of IC engine: Properties of steam, Phase transformation process
and its graphical representation on P-V, T-V & T-s diagram, Mollier diagram and Steam Tables,
Processes involving steam in closed and open systems. Introduction to I.C. Engines: Two & four stoke
S.I. and C.I. engines. Otto cycle, Diesel cycle, Dual cycle.

UNIT-4: Force system and Analysis: Force system and Analysis Basic concept: Review of laws of
motion, transfer of force to parallel position, resultant of planer force system, Free Body Diagrams,
Equilibrium. Friction:Introduction, Laws of Coulomb friction, Equilibrium of bodies involving dry fiction.
Structure Analysis Beams: Introduction, Shear force and bending moment, Shear force and bending
moment diagram for statically determinate beams. Trusses: Introduction, Simple Trusses, Determination
of forces in simple truss members, Method of joints and Method of section.

UNIT-5: Stress and Strain Analysis :Simple stress and strain: Introduction, Normal shear stresses,
Stress-strain diagrams for ductile and brittle materials, Elastic constants, One dimensional loading of
members of varying cross section, Strain energy, Thermal stresses. Compound stress and strains:
Introduction, State of plane stress, Principal stress and strain, Mohrs circle for stress and strain. Pure
Bending of Beams: Introduction, Simple bending theory, Stress in beams of different cross
sections.Torsion: Introduction, Torsion of Shafts of circular section, Torque and Twist, Shear stress due to
Torque
Reference:
1. Agarwal, Basic Mechanical Engineering, Wiley India
2. Holman, J.P. : Thermodynamics, McGraw Hill book Co. NY.
3. Singh Onkar, Bhavikatti S.S., Chandra Suresh : Introduction to Mechanical Engineering:
Thermodynamics, Mechanics and Strength of Materials, New Age International Publishers
4. Yadav R. : Thermodynamics and Heat Engines, Vol I& II (SI Edition) Central Publ. House.
36
FUNDAMENTALS OF COMPUTER & PROGRAMMING

Course Code: ECS 207 Credit Units: 04

Unit 1
Basics of Computer System

Block Diagram of a Computer, Booting Process, Types of Computer Systems, Introduction to the
concepts: Bit, Byte, Word, hardware, Operating System and Application Software, Compilers,
Assemblers, Loaders and Linkers.ASCII& EBCDIC codes, Binary, Octal, Decimal & Hexadecimal
Number Systems & their Conversions

Unit 2
Introduction to Computer Operating System
Features of DOS, Windows and UNIX Operating System and their Comparison, Concept of Flowchart
and Algorithm, Machine, Assembly and high level languages, Structured Programming, Use of high level
programming languages for the systematic development of programs.

Unit 3

C Fundamentals

Character Set, Identifiers & Keywords, Data Types, Constants, Variables, Tokens, Operators &
Expression, Library Functions, Statements, Symbolic Constants, Preprocessor Directives, Functions.

Unit 4

Arrays and Structure

Introduction, Classification of Array: One Dimensional Array, Multidimensional Array, Two Dimensional
Arrays.Defining a Structure, Array vs. Structure, Initialization of a Structure, Nested Structure

Unit 5
Pointers and File handling
Pointers Variables, Pointer Operator, Pointer Expression, Array of Pointers , Multiple Indirection,
Pointers to functions, dynamic allocation functions, File I/O: Streams and files, file System basic, fread,
fwrite ,fseeek, random access I/O, fprintf(), fscanf(), standard streams.

Reference Book

37
1 Let Us C :YashwantKanetkar [BPB]
1. Jones, C Programming with problem solving, Wiley India
2. Gupta: Computer Concepts & C Programming, Comdex
FUNDAMENTALS OF ELECTRONIC ENGINEERING

Course Code: EEC 208 Credit Units: 04

Unit 1 Semiconductor materials and properties :Group-IV materials, Covalent bond, electron-hole
concepts, Basic concepts of energy bands in materials, concepts of forbidden gap, Intrinsic and extrinsic
semiconductors, donors and acceptors impurities, Junction diode p-n junction, depletion layer, v- i
characteristics, dioderesistance, capacitance diode ratings ( average current, repetitive peak current,
non-repetitive current, peak-inverse voltage).
Unit-2 Diode Applications: Rectifiers (half wave and full wave), calculation of transformer utilization
factor and dioderatings, Introduction to filters, calculation of ripple factor and load regulation,clipping
circuits, clamping circuits, voltage multipliers

Breakdown diodesbreakdown mechanisms (zener and avalanche), breakdown characteristics,zener


resistance, zener diode ratings, zener diode application as shunt regulator

UNIT-3 Bipolar Junction Transistor : Basic construction, transistor action, CB, CE and CC
configurations, input/outputCharacteristics, concept of Biasing of transistors-fixed bias, emitter bias,
potential divider bias, Transistor Amplifier, Graphical analysis of CE amplifier, concept of voltage gain,
current gain, h-parameter model (low frequency), computation of Ai, Av, Ri, R O of single transistor CE
and CC amplifier configurations.

Unit-4 Field Effect Transistor : FET: Basic construction, transistor action, concept of pinch off,
maximum drain saturation current, input and transfer characteristics, characteristics equation CG, CS and
CD configurations, Introduction to self and fixed biasing .MOSFFT: depletion and enhancement type
MOSFET-construction, operation and characteristics. Computation of Av, Ri, Ro, of single FET
amplifiers using all the three configurations

Unit-5 Switching theory and logic design : Number systems, conversions of bases, Boolean algebra,
logic gates, concept of universal gate,concept of K- Map Operational Amplifiers: Concept of ideal
operational amplifiers, ideal op-amp parameters, inverting, non-inverting and unity gain amplifiers

Reference Books:
1.RobertBoylestad, Electronic Devices and Circuit theory, Pearsons Educations.
38
2. R. K. Singh &LalKishor, Basic Electronics Engineering, B S Publication, Hyderabad.
3. Gaikwad, Basic Electronics, Wiley India
4. A Mottershead, Electronic devices and circuits. PHI, 2000.
5. Morris Mano, Digital Computer Design, PHI, 2003.
ENVIRENVIRONMENTAL STUDIES

Course Code: EBT 209 Credit Units: 01

UNIT 1

NATURAL RESOURCES:

Renewable and Non-renewable Resources :


Natural resources and associated problems.

a) Forest resources : Use and over-exploitation, deforestation, case studies. Timber extraction,
mining, dams and their effects on forest and tribal people.

b) Water resources : Use and over-utilization of surface and ground water, floods, drought, conflicts
over water, dams-benefits and problems.

c) Mineral resources : Use and exploitation, environmental effects of extracting and using
mineral resources, case studies.

d) Food resources : World food problems, changes caused by agriculture and over-grazing, effects of
modern agriculture, fertilizer-pesticide problems, water logging, salinit y, case studies.

e) Energy resources : Growing energy needs, renewable and non renewable energy sources, use
of alternate energy sources. Case studies.

f) Land resources : Land as a resource, land degradation, man induced landslides, soil erosion
and desertification.
Role of an individual in conservation of natural resources.
Equitable use of resources for sustainable lifestyles.

39
UNIT 2
ECOSYSTEMS
Concept of an ecosystem.
Structure and function of an ecosystem.
Producers, consumers and decomposers.
Energy flow in the ecosystem.
Ecological succession.
Food chains, food webs and ecological pyramids.

Introduction, types, characteristic features, structure and function of the following ecosystems :-
a. Forest ecosystem

b. Grassland ecosystem
c. Desert ecosystem
d. Aquatic ecosystems (ponds, streams, lakes, rivers, oceans, estuaries)

UNIT 3

BIODIVERSITY AND ITS CONSERVATION


Introduction Definition : genetic, species and ecosystem diversity.
Biogeographical classification of India

Value of biodiversity : consumptive use, productive use, social, ethical, aesthetic


and option values
Biodiversit y at global, National and local levels.
Inida as a mega-diversit y nation
Hot-sports of biodiversity.

Threats to biodiversit y : habitat loss, poaching of wildlife, man-wildlife conflicts. Endangered and
endemic species of India
Conservation of biodiversit y : In-situ and Ex-situconservation of biodiversity.
UNIT 4

ENVIRONMENTAL POLLUTION & SOCIAL ISSUES


Definition
Cause, effects and control measures of :-
a. Air pollution
b. Water pollution
c. Soil pollution
d. Marine pollution
e. Noise pollution
f. Thermal pollution
g. Nuclear hazards
h Disaster management : floods, earthquake, cyclone and landslides.
i. From Unsustainable to Sustainable development
j. Urban problems related to energy
k Water conservation, rain water harvesting, watershed management
l. Environmental ethics : Issues and possible solutions.

m. Climate change, global warming, acid rain, ozone layer depletion,


nuclear accidents and holocaust. Case Studies.
n. Wasteland reclamation.
o. Consumerism and waste products.

REFERENCE BOOK
1. MAjni Reddy, Text book of environmental Science, BS Publication, Hyderabad
2. Environmental Studies by Daniel, Wiley India
3. Environmental Studies by ErachBharucha, University Press.
4. Fundamental of Ecology, E.P.Odum, Cengage Learning.
5. Environmental Science and Engineering by Wright, Pearson Publication.6.
6. First Ecology by Beeby and Brennan, Oxford University Press.
41
7. Environment Science by Miller, Cengage Learning, New Delhi.

8. Hand book of Environmental laws, Rules, Guidelines, Compliances and Standards Vol. 1 & Vol. 2,
Bharat Publication, New Delhi.

42
INDUSTRIAL COMMUNICATION-II

Course Code: ASH204 Credit Units: 02

Objective: Second module functions to enhance the ability of Reading skills and develop an attitude
towards language learning and comprehension skills.

Unit-1: Learning Reading Skills


Importance and need of Reading Skills
Types of Reading Skills
Methods & objectives of Reading Skills

Unit-2: Jargon & Vocabulary building


Examples of jargon
Technical Terminology
One word Vocabulary
Prefixes, Suffixes, Homophones
Synonyms & Antonyms

Unit-3: Biographical reading


Reading biography of Mahatma Gandhi
Discourse sessions
Summary of reading
Meaning of difficult words

Unit -4: English Literature


Animal Farm George Orwell (Drama)
Tintern Abbey William Wordsworth (poem)
Of Studies Francis Bacon(Essay)

43
PHYSICS PRACTICALS

Course Code: ASH 222 Credit Units: 01

List of Experiments (At least Ten)

1. To determine the wavelength of monochromatic light by Newtons ring.

2. To determine the wavelength of monochromatic light with the help of Fresnelsbiprism.

3. To determine the focal length of two lenses by nodal slide and locate the position of cardinal points.

4. To determine the specific rotation of cane sugar solution using half shade polarimeter.

5. To determine the wavelength of spectral lines using plane transmission grating.

6. To determine the specific resistance of the material of given wire using Carey Fosters bridge.

7. To determine the variation of magnetic field along the axis of a current carrying coil and then to
estimate the radius of the coil.

8. To verify Stefans Law by electrical method.

9. To calibrate the given ammeter and voltmeter.

10. To study the Hall Effect and determine Hall coefficient, carnierd ensityand mobility of a given
semiconductor material using Hall-effect set up.

11. To determine energy bank gap of a given semiconductor material.

12. To determine E.C.E. of copper using Tangent or Helmholtz galvanometer.

13. To draw hysteresis curve of a given sample of ferromagnetic material and from this to determine
magnetic susceptibility and permeability of the given specimen.

14. To determine the balistic constant of a ballistic galvanometer.

15. To determine the viscosity of a liquid.

Note : Additional experiments may be added based on contents of syllabus.

44
CHEMISTRY LAB

Course Code: ASH 223 Credit Units: 01

LIST OF EXPERIMENTS ( AT LEAST TEN)

1. Determination of alkalinity in the given water sample.

2. Determination of temporary and permanent hardness in water sampleusing EDTA asstandard solution.

3. Determination of available chlorine in bleaching powder.

4. Determination of chloride content in the given water sample by Mohrs method.

5. Determination of iron content in the given ore by using external indictor

6. pH-metric titration.

7. Determination of Equivalent weight of Iron by the chemical displacement method. The equivalent
weight of copper is 63.5

(Note : the procedure to be followed in carrying the above experiment is given as annexure)

8. Viscosity of an addition polymer like polyster by Viscometer.

9. Determination of iron concentration in sample of water by colorimetric method. The method involves
the use of KCNS as colour develop ingagent and the measurements are carried out at l max 480 nm.

Note : The general procedure of estimation is given on pp653-8 of thetextbook of Quantitative Chemical
Analysis by A.I. Vogel 6th Edition,Publisher : Pearson education Ltd. 2000)

10. Determination of heat of neutralization of Hydrochloric acid andSodium hydroxide

11. Determination of amount of dissolved oxygen in water

12. Separation of metal ions by paper chromatography

13. Preparation of iodoform from acetone

14. Preparation of Sodium Cobaltinitrile salt.

15. Element detection & functional group identification in organiccompounds

45
BASIC ELECTRICAL LAB

Course Code: EEL225 Credit Units: 01

List of experiments (Any Ten)

1. Verification of Kirchhoffs Law (KVL & KCL).

2. Study of phenomeno n of resonance inRLC series circuit.

3. Verification of superposition theorem

4. Verification of Thevenins theorem.

5. Verification of Nortons theorem.

6. Verification of maximum power transfer theorem.

7. Measurement of power in a three phase circuit by two wattmeter method.

8. Measurement of efficiency of a single phase transformer by load test.

9. Determination of parameters and losses in a single phase transformerby OC and SC test.

10. Study running and reversing of a three phase induction motor.

11. Study of a single phase energy meter.

46
MECHANICAL ENGINEERING LAB

Course Code: EME-226 Credit Units: 01

A minimum of 10 experiments from the following :

1. Study of Steam engine and steam turbine models.

2. Study of 2-stroke and 4 -stroke I.C Engine models.

3. Study of a vapor compression Refrigeration unit tutor/refrigerator.

4. Study of a window type air conditioner.

5. To conduct the tensile test on a UTM and determine ultimate Tensile strength, percentage elongation
for a steel specimen.

6. To conduct the compression test and determine the ultimate compressive strength for a specimen.

7. To conduct the Impact test (Izod / charpy) on the Impact testing machine and to find the impact
strength.

8. To determine the value of acceleration due to gravity by Atwoods Machine appratus.

9. To verify the principle of moment by Bell Crank Lever Appratus

10. To determine the moment of inertia of a flywheel apparatus about its axis of rotation

11. To verify Newtons second law of motion by Fletchers Trolley apparatus

12. To find out coefficient of friction by combined inclined plane & friction slide apparatus

13. To determine the velocity ratio, mechanical advantage & efficiency of a single purchase crab
apparatus & draw graph between load vs effort, mechanical advantage and efficiency.

14. To determine the velocity ratio, mechanical advantage & efficiency of a double purchase crab
apparatus.

47
FUNDAMENTALS OF COMPUTER PROGRAMMING LAB

Course Code: ECP-2 27 Credit Units: 01

List of Practical

1. Practice of some internal and External DOS Commands

2. WAP to determine whether given number is even or odd

3. File and program management in windows

4. WAP to find hexadecimal and octal value of a number

5. WAP to swap two numbers

6. WAP to find sum of all the digits of a number

7. TO simulate calculator the perform the following operations on two numbers: Addition, subtraction,
Multiplication, division.

8. WAP to read two matrix and multiply them

9. WAp to make a copy of a text file using EOF,FGETC and FPUTE

10. WAP to generate table of any number using pointer

11. WAP to fid wheather given number is Pallondroning or not.

12. WAP to calculate the simple interest for a set of values representing principle, number of years
and rate of interest.

Note: List may be modified according to new software available

48
FUNDAMENTALS OF BASIC ELECTRONIC ENGINEERING LAB

Course Code: EEC-228 Credit Units: 01

Fundamentals of electronic Lab (Any Ten)

1. Study of diode characteristics.

2. To study a half wave and full wave rectifier circuit.

3. Determination of ripple factor of capacitive and non capacitive filter for HWand FW diode
rectifier circuit.

4. Study of characteristics of Zener Diode as constant voltage.

5. Verification of Application of Zener Diode as shunt regulator.

6. Study of Clipper and Clamper Circuit with different waveforms.

7. Determination of characteristics of BJT in CB and CE configuration.

8. Determination of characteristics of FET in CS and CD configuration.

9. Study of BJT as single stage amplifier and determination of Ai, Vi, Ri and Ro.

10. Study of AND, NAND, OR, NOR and EXOR gates.

11. Verification of Universal gates.

49
WORKSHOP PRACTICE

Course Code: EWS 210 Credit Units: 01

1. Carpentry Shop: 1. Study of tools and operation and carpentry joints. 2. Simple exercise
using jack plain. 3. To prepare half- lap corner joint, mortise and tennon joints. 4. Simple
exercise on woodworking lathe.
2. Fitting Bench Working Shop : 1. Study of tools and operations 2. Simple exercises
involving filling work. 3. Making perfect male-female joint 4. Simple exercise involving
drilling/tapping/dieing.
3. Black Smithy Shop: 1. Study of tools and operations 2. Simple exercises based on black smithy
operations such as upsetting, drawing down, punching, bending, fullering& swaging.
4. Welding Shop: 1. Study of tools and operations . 2. Simple butt joint. 3. Lap joint. 4.
oxy acetylene welding.
5. Sheet metal shop: 1.Study of tools and operations. 2. Making funnel complete with soldering.
3. Fabrication of tool box, tray, electrical panel box etc.
6. Machine Shop: 1. Study of tools and operations. 2. Plane turning. 3. Step turning. 4. Taper
turning 5. Threading. 6. Single point cutting tool grinding.

Reference:

1. Hajra, Bose, Roy: Workshop Technology Vol 1 & 2, Media Promotors


2. Raghuvanshi B.S.: Workshop Technology, Vol 1 & 2, Dhanpatrai

50
ENGINEERING DRAWING

Course Code: EED 211 Credit Units: 01

1. Graphics as a tool to communicate ideas, Lettering and dimensioning, Construction of


geometrical figures like pentagon and hexagon.

2. Orthographic Projection Principles of orthographic projections, Principal and auxiliary planes,


First and Third angleprojections.Projection of points.Pictorialview.Projection of lines parallel to
both the planes.Parallel to one and inclined to other, Inclined to both the planes. Application to
practical problems.Projection of solid in simple position, Axis or slant edge inclined to one and
parallel to other plane,Solids lying on a face or generator on a plane.Sectioning of solids lying in
various positions, True shape of the section.Development of lateral surfaces, sheet metal drawing.

3. Isometric Projection Principles of isometric projection, Isometric projection using box and offset
methods.

Reference books:

1. Bhatt. N.D.: Elementary Engineering Drawing, Charothar Publishing.

2. D A Hindoliya, Text book of Egg. Graphics, BS Publication, Hyderabad

51
CELL BIOLOGY

Course Code: EBT 301 Course Credits: 04

Unit I
Cell: An introduction, classification of organisms by cell structure, cytosol, compartmentalization of
eukaryotic cells, cell fractionation.

Unit II
Cell membrane and permeability: Chemical components of biological membranes, organization and
fluidity of membrane components, the membrane as a dynamic entity, cell recognition and membrane
transport.

Unit III
Organization of transport activity in cell. Signal Tans duction. Cell signaling: Types of signaling,Cell
surface receptor mediated signaling (RTK, pathway, JAK-STATpathway), G-proteins and G-protein
coupled receptors, Secondary messengers and intracellular communication, Target cell adaptation.

Unit IV
Cytoskeleton and cell motility: Structure and functions of microtubules, microfilaments, intermediate
filaments. Structure and Functions of Cellular Organelles: Endoplasmic reticulum, golgi complex,
lysosomes, vacuoles and microbodies, ribosomes, mitochondria, plastids.

Unit V
Nucleus: Structure, cell-cycle (interphase and M phases), regulation of cell cycle.
Extra cellular matrix: Composition, molecules that mediate cell adhesion, membrane receptors
for extra cellular matrix macromolecules, regulation of receptor expression and function
Text/ References Books:
th
1. Molecular Biology of cell, 4 ed.Alberts,Bruce (etal)(2002) Garland Science Publishing, New
York.
2. Cell Biology-Smith and Wood by Chapmanand Hall.
3. Cell Biology: Organelle structure and function, Sadava,
DE.(2004) Panimapub., NewDelhi.
th
4. Cell and Molecular Biology, 8 ed.Robertis, EdpDeandRobertis, EmfDe(2002) Lippincot
Williams and Wilkins Pvt.Ltd., (International Student Edition) Philadelphia.
th
5. Molecular Cell Biology 4 ed.Lodish, Harvey and Baltimore, D (2000)W.H.freeman & Co.
New York

52
BIOCHEMISTRY-I

Course Code: EBT 302 Course Credits: 04

Unit I
Hydrogen bonding and structure of water molecule, lionization of water, pH, Buffers. Colligative
properties of water. Carbohydrates-Structure and functions: Structures and properties of mono
saccharides, oligosaccharides and polysaccharides. Ring structure and mutarotation. Homo - and
hetero- polysaccharides. Mucopolysaccharides.

Unit II
Amino acids & Proteins: Structure and properties of amino acids. Essential and non-essential amino
acids. Peptide bonds. Types of proteins and their classification. Forces stabilizing protein structure and
shape. Different levels of structural organization of proteins. Lipids-Structure and functions:
Classification of lipids and their general functions. Essential fatty acids. Hydrolysis of fats,
Saponification value, Rancidity of fats, Cholesterol- its structure and biological functions.

Unit III
Nucleic Acids-Structure and functions: Structure and properties of purine and pyrimidinebases.
Nucleosides and nucleotides. Biologically important nucleotides. Vitamins: Role of Vitamins, metals
ions, significance.

Unit IV
Metabolism: Basicconcepts, Anabolism and catabolism, Carbohydrate Metabolism: Glycolysis. Fate of
pyruvate under aerobic and anaerobic conditions. Pentose phosphate pathway and its significance.
Gluconeogenesis pathway. Maintenance of blood glucose level. Energetics and regulation of
carbohydrate metabolism. Lipid Metabolism: Beta-oxidation of saturated fatty acids, oxidation of
unsaturated and odd carbon fattyacids. Formation and utilization of ketone bodies.

Unit V
Amino Acid Metabolism: General reactions of amino acids metabolism-transamination, oxidative and
non-oxidative deamination and decarboxylation. Urea cycle and its regulations. Nitrogencycle.
Nucleic Acid Metabolism: Catabolism, de novo-biosynthesis and regulation of purine and pyrimidine
nucleotides. Formation of deoxy ribbo nucleotides.
Recommended Books:
1. Principles of Biochemistry: A.L. Lehninger, Nelsonand Cox, McMillan Worth Publishers.
2. Biochemistry: Voetand Voet, John Wiley and Sons, Inc. USA.
3. Biophysical Chemistry Vol.I,II&III: Cantorand Schimel, Freeman.

53
4. Biochemistry: Zubey, WCB.

5. Biochemistry: Garrett and Grisham, Harcourt.

6. Biochemistry: Stryer, W. H. Freeman.

7. Understanding Enzymes:T. Palmer, Horwood.

8. Harper's review of Biochemistry: R.K. Murrayetal., Prentice-HallInternationalInc.

9. Fundamentals of Biochemistry: Cohn and Stumf.

54
MICROBIOLOGY

Course Code: EBT 303 Course Credits: 04

Unit 1
Development of microbiology, scope and relevance of microbiology, Fieldsin microbiology, History of
Microbiology: Contribution of Koch, Contribution of Lister, germ theory of fermentation, Conflicts of
Biogenesis and Abiogenesis, Contribution of Met kinik offs.

ClassificationofMicroorganism,TwoKingdomclassification,Threekingdomclassification,Five Kingdom
classification,Eightkingdomclassification.DifferencebetweenEukaryoteand Procaryote, method of
classification based on 16sRNA, DNA homology, Identification of BacteriaBiochemicaland molecular
Approach.

Unit II
Morphology and ultra structureofbacteria.Morphologicaltypes.Cellwallofarchaebacteria, Gram,
negative and Gram-positivebacteria,eukaryotes.L-formcellwallsynthesis.Capsules- types composition
and function. Cell membranes- structure, composition and properties. Cultivation of aerobic and
anaerobic bacteria.

Unit III
Structure and function of flagella, cilia, pili, gas vesicles, chromosomes, carboxysomes,
magnetosomesandphycobilisomes, nucleoid, cell division. Spores, reservefood materials
polyhydroxybutyrate, phosphate granules. Oil droplets, cyanophycingranulesand sulfur inclusions.

Unit IV
Cultivation of bacteriaaerobic, anaerobic, nutritional types, culture media used, growth curve
generation time. Growth kinetics, asynchronous and synchronous growth Batchandcontinuous cultures.
Measurement of growth, factors affecting growth. Control of bacteria-
physicalandchemicalagents.IsolationofPureculture,Preservation methods. Sterilising techniques,

Unit V
Virology: Structure and cultivation of Virus, General, Brief outline on discovery of viruses.and. Types
of envelopes and their compositions. Viral genome, their types structures,virusrelatedagents (viroids
virusoids and prions).Bacteriophage and its lifecycle.

Recommended Books

1. Text book o fMicrobiology: R.C. Dubey and D.K.Maheshwari


2. Text book of Microbiology: Annathnarayan and Panicer
3. Microbiology; Prescott
4. Practical Microbiology: .Dubey and D.K.Maheshwari

55
BASIC BIO ANALYTICAL TECHNIQUES

Course Code: EBT 304 Course Credits: 04

Unit I:
Introduction
Types of Analytical Methods, Instrument for analysis, Uncertainties in Instrumental measurements
Sensitivity and detection limit for instruments, pH meter, dissolved oxygen, agitation, sensors and their
operation.

Unit II: Microscopy:


Brightfield, Dark field, Fluorescent, Phase contrast, Confocal microscopy, SEM and TEM
microscopy, Flowcytometry.

Unit III: Centrifugation


General principles, Ultracentrifugation, Velocity Sedimentation and Measurements,
Equilibrium Ultra centrifugation-Density Gradient Centrifugation.

Unit IV: Spectroscopy


General Principles-Radiation, energy and atomic Structure-types of spectra and their biochemical
Usefulness-basic laws of light absorption. Electromagnetic radiation and spectrum, Beer- Lamberts law and
apparent deviations; UV-VIS spectrophotometer, Atomic absorption and Atomic emission spectroscopy,
Circular Dichroism (CD)-principles, instrumentation and applications, X-ray diffraction and
crystallography: principle, mode of operation and application.

Unit V: Separation Equipments-Principle and Operation

HPLC, Gas Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography, Affinity
Chromatography, Membrane separation, Ultrafilteration, Reverse Osmosis.

Text Books
1. AbiologistGuidetoprinciplesandtechniqueofpracticalbiochemistry-ByKeithWilson, Kenneth
rd
H. Gouldind3 Edition,ELBS Series
2. SkoogandWest, Fundamentals of Analytical Chemistry, 1982.
3. Vogel, Text Book ofQuantitativeInorganic analysis, 1990.
4. Ewing,Instrumental Method of analysis, 1992
5. HobertHWillard,D.L.MerrittandJ.R.J.A.Dean,instrumentalmethodsofanalysis,CBS Publishers
and Distributors, 1992
6. F.Settle.HandBookofInstrumentalTechniquesforAnalyticalchemistry,PrenticeHall,
1997.

56
COMPUTERBASED NUMERICAL ANDSTATISTICAL TECHNIQUES

Course Code: EBT 305 Course Credits: 04

Unit-I
Floating point Arithmetic: Representation of floating point numbers, Operations, Normalization, Pitfalls of
floating point representation, Errors in numerical computation. Iterative Methods: Zerosofa single
transcendentalequationandzerosofpolynomialusingBisectionMethod,Iteration Method, RegulaFalsi method,
Newton Raphson method,Secant method, Rate of convergence of iterative methods.

Unit-II
Simultaneous Linear Equations: Solutions of system of Linear equations, Gauss Elimination directmethod and
pivoting ,Ill Conditioned system of equations, Refinement of solution.Gauss Seida literative method, Rate of
Convergence. Interpolation and approximation: Finite Differences, Difference tables, Polynomial Inter
polation: Newtons forward and backward formula,Central Difference Formulae: Gauss forward and backward
formula, Stirlings, Bessels, Everettsformula.
Interpolation with unequalintervals: Langranges Inter polation, Newton Divided difference formula,
HermitesInterpolation,Approximationoffunctionby Taylors series and Chebyshev polynomial

Unit-III
Numerical Differentiation and Integration:
Introduction, Numerical Differentiation, Numerical Integration, Trapezoidal rule, Simpsons rules, Booles
Rule, Weddles RuleEulerMaclaurin Formula.
Solution of differential equations:
Picards Method, Eulers Method, Taylors Method, RungeKutta methods, Predictorcorrector method,
Automatic error monitoring, stability of solution.

Unit-IV
Curvefitting, Cubic Spline and Approximation:Method of least squares,fittingofstraight lines, polynomials,
exponential curvesetc. Frequency Chart: Different frequencychart like Histogram,Frequencycurve, Pichart.
Regression analysis: Linear and Nonlinearregression, multiple regressions.

References:
1. RajaramanV.,ComputerOrientedNumerical Methods, PHI
2. Gerald&Wheatley, Applied Numerical Analyses, AW
3. Jain, Iyengar and Jain, Numerical Methods forScientific and EngineeringComputations, NewAgeInt.
4. GrewalB. S., Numerical methods in Engineeringand Science, KhannaPublishers, Delhi
5. T. Veerarajan, T Ramachandran,TheoryandProblems in Numerical Methods, TMH
6. PradipNiyogi, Numerical Analysis and Algorithms, TMH

57
Industrial Communication-III
Course Code: ASH 304 Credits:02

Objective: Third Module improves the writing ability to produce Technical and nontechnical documents
Unit-1: Learning Writing Skills
Definition and Introduction
Importance of Writing Skills
Types of Writing of Skills
Techniques of Writing Skills
Objectives of Improving Writing Skills
Methods of improving Writing Skills

Unit-2: Paragraph Writing


Paragraph
Components of paragraph writing
Methods of paragraph writing
The Art of Condensation
Steps of effective prcis writing
Difference between Summary and Abstract
Unit-3: Formal and Informal letters
Formal letters
Types of formal letters
Informal letters
Types of Informal letters
Letter writing practice
Unit-4: English Literature
The Diary of a Young Girl Anne Frank (Novel)
London - William Blake (Poem)
Of Discourse Francis Bacon (Essay)

58
CELL BIOLOGY
Course Code: EBT 321 Course Credits:01

List of experiments

Module I: Study of microscopy.

Module II: Micrometer (To measure the length and breadth of the given cell sample by using micrometer.).

Module III: To prepare a temporary stained mount of onion peel to see the structure of cells under the
microscope.

Module IV: Permanent slide preparation (To prepare permanent slides using the given sections like Stem,

Root and Leaf.).

Module V: To identify the different types of cells present in the leaf cross section.

Module VI: Preparation of wax embedded slides and tissue sectioning with the help of microtome.

Module VII: Cell Counting and viability (To identify the number of cells present in the given 1ml sample).

Module VIII: Study of mitosis.

Module IX: Study of meiosis.

Module X: To isolate the mitochondria from the given sample.

59
BIOCHEMISTRY LAB
Course Code: EBT 322 Course Credits:01

List of experiments

Module I: Qualitative analysis of proteins.

Module II: Qualitative analysis of carbohydrates.

Module III: Qualitative analysis of lipids.

Module IV: Estimation of pH of Different solutions and buffers by digital pH Meter.

Module V: Preparation and purification of casein from buffalo milk.

Module VI: Determination of acid value of fat/oil.

Module VII: Determination of Iodine number of fat/oil.

Module VIII: Determination of Logic properties (pH value of Lysine by titration).

Module IX: To find lambda max for proteins.

Module X :. Estimation of protein by lowreys method

60
MICRO BIOLOGY LAB

Course Code: EBT 323 Course Credits:01

Module I: Preparation of solid and liquid media.

Module II: Isolation and maintenance of organisms by plating, streaking and serial dilution.

Module III: Preparation of slant cultures.

Module IV: Growth curve measurement of bacterial population by turbidometry.

Module V: Measurement of bacterial population by dilution method.

Module VI: Effect of temperature, pH, carbon and nitrogen sources on growth of bacteria.

Module VII: Microscopic examination of bacteria by gram staining.

Module VIII: Endospore staining.

Module IX: Capsule staining.

Module X: Isolation and identification of Rhizobium from root nodules.

61
BIOANALYTICAL LAB PRACTICALS

Course Code: EBT 322 Course Credits:01

List of experiments

Module I: To study the working and principle of instrument used in the Basic bio analytical technique lab.

Module II: Demonstration of mitotic cell using microscopy

Module III: Verification of Lambert Beers Law by UV Vis Spectrophotometer.

Module IV: Estimation of Different macromolecules by visible spectrophotometer.

Module V: Estimation of nucleic acids by UV method.

Module VI: Estimation of proteins by UV method.

Module VII: Separation of different macromolecules by paper chromatography.

Module VIII: Separation of different macromolecules by Thin Layer Chromatography.

Module IX: To study different cell lysis methods.

Module X: Cell Fractionation.

62
FOOD BIOTECHNOLOGY

Course Code: EBT 401 Course Units: 04

Unit: 1

History of Microorganisms in food, Historical Developments. Role and significance of microorganisms in


foods. Intrinsic and Extrinsic parameters of Foods that affect microbial growth. Basic principles, unit
operations, and equipment involved in the commercially important food processing methods and unit
operations

Unit: 2

Microorganisms in fresh meats and poultry, processed meats, seafoods, fermented and fermented dairy
products and miscellaneous food products. Starter cultures, cheeses, beer, wine and distilled spirits, SCP,
medical foods, probiotics and health benefits of fermented milk and foods products. Brewing, malting,
mashing, hops, primary & secondary fermentation: Biotechnological improvements: catabolic repression,
High gravity brewing, B-glucan problem, getting rid of diacetyl. Beer, wine and distilled spirits.

Unit: 3

Nutritional boosts and flavor enhancers: Emerging processing and preservation technologies for milk and
dairy products. Microbiological Examination of surfaces, Air Sampling, Metabolically Injured
Organisms.Enumeration and Detection of Food-borne Organisms. Bioassay and related Methods

Unit: 4

Food Preservation, Food Preservation Using Irradiation, Characteristics of Radiations of Interest in Food
Preservation. Principles Underlying the Destruction of Microorganisms by Irradiation, Processing of Foods
for Irradiation, Application of Radiation, Radappertization, Radicidation, and Radurization of Foods Legal
Status of Food Irradiation, Effect of Irradiation of Food constituents.

Unit: 5

Storage Stability Food Preservation with Low Temperatures, Food Preservation with High Temperatures,
Preservation of Foods by Drying, Indicator and Food-borne Pathogens, Other Proven and Suspected Food-
borne Pathogens. Rheology of Food Production.

Text / Reference Books:

1. Frazier, W.S. and Weshoff, D.C., 1988. Food Microbiology, 4th Edn., McGraw Hill Book Co.,
New York.
2. Mann &Trusswell , 2007. Essentials of human nutrition. 3rd edition .oxford university press. 3. Jay,
J.M., 1987. Modern Food Microbiology, CBS Publications, New Delhi. 4 Lindsay, 1988. Applied
Science Biotechnology. Challenges for the flavour and Food Industry. Willis Elsevier. 5. Roger,
A., Gordon, B. and John, T., 1989. Food Biotechnology.

63
GENETICS

Course Code: EBT 402 Course Units: 04

Course Objective:

The objective of the course is to focus on the basic principles of genetics incorporating the concepts of
classical, molecular and population genetics. Compilation is required for recent advances in genetic
principles for strong foundation in Biotechnology.

Course Contents:

Module I

The science of genetics -introduction, history, classical and molecular genetics, role of genetics in
medicine, agriculture and society.

Module II

Mendelian law of inheritance and its applications, Extension of Mendelism - Allelic variations, influence of
environment on expression, penetrance and expressivity, epistasis, pleiotropy. Pedigree analysis;
Chromosomal basis of inheritance; sex linkage,, crossing over and chromosome mapping in eukaryotes.

Module III

Genetic code and protein biosynthesis; Gene regulation, Genes in development; Extra chromosomal
inheritance, Male sterility and incompatibility; Recombination in bacteria, fungi and viruses, tetrad analysis.

Module IV

Numerical changes and structural changes in chromosomes with emphasis on human disease/syndromes.

Module V

Mutation and mutagenic agents, types of mutations; Transposons: types of transposons; Concept of gene
classical and modern, psendoallelism, position effect, intragenic crossing over & complementation.

Module VI

Population genetics; Genetic drift, Hardy- Weinburg Law and its deviations.

Text & References:

1.Genetics, P.K. Gupta, Rastogi Publication


2.Concepts of Genetics (Sixth Edition), William S. Klug and Michael R, Cummings, Pearson Education

References:
1. Genetics, M.W. Strickberger, Prentice Hall College Division

64
CELL & TISSUE CULTURE TECHNIQUES

Course Code: EBT 403 Course Units: 04

Unit 1: Introduction to animal tissue culture: Background, advantages, limitations, application, major
differences in vitro, types of tissue culture, Initiation of the culture: Culture environment, Origin and
evolution of cell lines, cell proliferation, cell adhesion, cell differentiation and micro-environment. Basic
techniques of cell cultures: Primary and secondary cell culture, Growth factors used in culture,

Unit 2: Cell lines: Preparation of medium, nomenclature, subculture and propagation, immortalization of
cell lines, designations, routine maintenance, mass culture techniques. Characterization and quantification
of Cell line: Direct and indirect methods for quantification, characterization of cultured cells, morphology,
parameter of growth, phases of cell growth, multiplication rate, application of animal cell culture and stem
cell culture.
Preservation and contamination: Need of cryopreservation, source of contamination, type of microbial
contamination, monitoring, eradication of contamination and cross-contamination. Cell Banking, Gene
transfer and transfection procedures.

Unit 4: Classical vs. modern approach of Plant tissue Culture. Plasticity and totipotency, plant cell culture
media, plant growth regulators. Protoplast Related Techniques Protoplast, Isolation, Culture and fusion,
Selection of hybrid cells, regeneration of hybrid plants, somatic hybridization and cybridization,
Applications in crop improvement. Plant regeneration: somatic embryogenesis and organogenesis. Culture
types, callus, cell-suspension cultures, root culture, shoot tip and meristem culture, embryo culture,
microspore culture.

Unit 5: Tissue Culture as some of Genetic Variability Somaclonal and gametoclonal variation, Selection,
Sources and causes of variation, Application in crop improvement. Tissue culture in Production of Haploids
and methods of diplodization, triploids, embryo rescue and development of virus free plants

Text Books /Reference: Animal cell culture

Animal Cell Culture by John R.W. Masters Oxford University Press


Introduction to Cell and Tissue Culture by Jennie P. Mather and Penelope E. Roberts
Plenum Press, New York and London
Molecular Biotechnology: Primrose.
Animal Cell Biotechnology: R.E. Spier and J.B. Griffiths (1988), Academic press.
Textbook of Biotechnology: H.K. Das

Text Books/Reference: Plant cell culture

Plant Tissue Culture: Applications and Limitations. S.S. Bhojwani (1990), Elsevier, Amsterdam.
Micropropagation: P.C. Debergh and R.H. Zimmerman (1990), Kluwer Academic Publ. Dordrecht.
Transgenic plants Lindsey and Jones
Plants, genes & crop improvement, Crispeels ASPB, 2002
Agricultural Biotechnology A. Altman.

65
DATA STRUCTURE AND ALGORITHMS

Course Code: EBT 404 Course Units: 04

Unit-I: Introduction to data structure and Algorithms

Performance analysis of Algorithm, time complexity, Big-oh notation, Elementary data organization data
structure operations, Recurrences, Arrays, Operation on arrays, representation of arrays in memory,
single dimensional and multidimensional arrays, spare matrices, Character storing in C, String operations.

Unit-II: Stacks, Quesues and Linked Lists

Stack operation, PUSH and POP, Array representation of stacks, Operation associated with stacks
Application of stacks, Recursion, Polish experession, Representation quesuesopration on quesues, Priority
quesuesDquesues, Singly and circularly linked list, List operations Lists implementations.

Unit-III: Tree

Basic terminology, Binary Trees, Binary tree representation, Algebraic/expressions, Complete Binary
Trees, Extended binary tree, representing binary tress in memory, linked representation of Binary trees,
Traversing binary trees & Searching in binary trees, Inserting in binary search trees, Complexity of
searching algorithm, Heaps, general trees, Threaded binary tree.

Unit-IV: Graphs

Terminology & representations, Graphs &Multigraphs, Directed Graphs, Sequential representation of


graphs, adjacency Matrices, Transversal, connected component and spanning trees, Minimum Cost
spanning tree, Prims and Kruskal Algorithm, BFS, DFS, Shortest path and transitive closure, Activity
networks, topological sort and critical paths.

Unit-V: Searching and Sorting

Linear search, binary Search, Internal and External sorting, Bubble sorting, selection sort, Insertion sort,
quick sort, Two way merge sort, Heap sort, sorting on different keys, practical consideration for internal
sorting, External Sorting, Storage Devices : Magnetic tapes, Disk Storage, Sorting with disks and
Indexing techniques, introduction to B tree and B+ tree, File organization and storage management,
Introduction to hoisting.

Text & References:

Text:
1. Data Structures and Algorithms, A.V. Aho, J.E. Hopcroft and J. Ullman, Addison-
Wesley Publishing
2. Database Design, Development and Deployment with Student CD, P. Rob and E. Semaan, McGraw-
Hill/Irwin
3. Schaums Outline of Data Structures with C++, J.R. Hubbard, McGraw Hill Trade.

References:
1.Database system concepts, A. Silberschatz, P.B. Galvin and G. Gagne, John Wiley and Sons Inc.
2. Introduction to Data Structures and Application, J. Tremblay and P.G. Sorensen, McGraw
Hill College Division
66
Bioprocess Engineering

Course Code: EBT 405 Course Units: 04

Unit I
Methods of inoculation and medium preparation, media design and optimization. Microbial growth in
closed, semi-open and open cultivation systems. Maintenance energy and yield concepts, parameters of
growth and analysis of growth data, estimation of biomass.

Unit II
Sterilization: concept and methods. Sterilization of medium. Kinetics of thermal death of microorganisms.
Batch sterilization. Continuous sterilization. Sterilization of air: Methods, filters and design of depth filters.

Unit III
Microbial kinetics of growth and substrate utilization. Product formation in batch, plug flow and chemostat
culture., Microbial pellet formation. Flocculation. Kinetics and dynamics of pallet formation.

Unit IV
Material and energy balance in steady and unsteady reaction systems. Oxygen transfer in bioreactors,
measurement of KLa. Oxygen transfer in large vessels. Control of physical, chemical and biological
environment of the bioreactor. Advanced control strategies viz. PID controllers, fuzzy logic based
controllers and artificial neural network based controllers, Role of physical, chemical & biological sensors.

Text books and references:


1. Principles of Microbe and cell cultivation- S. John Pirt, Butterworth Publication.
2. Bioprocess Engineering Principles P. M. Doran, 5th ed.
3. Hand Book Of Bioengineering- Skalak R & Shu Chien, 4th ed.
4. Biochemical Engg. Bailly & Ollis, Academic Press
5. Introduction to Chemical Engg. Series, MCH Int. Series.

67
INDUSTRIAL COMMUNICATION-IV

Course Code: ASH 404 Credit Units: 02

Objective: The Module improves ability to interact, share and respond it helps to overcome stage fear and
command over language.

Unit -1: Learning speaking Skills


Introduction & objective of speaking skills
Importance of speaking skills
Types of speaking skills
Objectives of improving speaking skills
Exercises

Unit-2: General Speaking skills


News Reading
Debate
JAM(Just a minute)
Extempore
Impromptu

Unit-3: Presentation skills


Define Presentation
Purpose of Presentation
Audience and Locale
Preparation of outline
Nuances of delivery
Kinesics
Paralinguistic
Chronemics
Proxemics

Unit -4: English Literature


Untouchable - Mulk raj Anand (Novel)
Obituary A.K Ramanujan (Poetry)
An Introduction Kamala Das (Poem)

68
FOOD TECHNOLOGY LAB

Course Code: EBT 421 Course Units: 01

Course Contents:

Module I: Estimation of ascorbic acid from given food sample by titrimetric method.

Module II: Analysis of Yeast and mold in contaminated food material.

Module III: Microscopic examination of Milk by breed method.

Module IV: Microbiological examination of foods.

Module V: Quality characterization of pasteurized milk by MBRT method.

Module VI: Estimation of lactose from milk.

Module VII: Detection of microbial count in Milk by SPC method.

Module VIII: Determination of Minimum Inhibitory Concentration (MIC) of Antibiotic.

69
CELL AND TISSUE CULTURE TECHNIQUES LAB

Course Code: EBT423 Course Credits: 01

List of experiments:

Module I: Aseptic culture techniques for establishment and maintenance of cultures.

Module II: Preparation of stock solutions of MS (Murashige & Skoog, 1962) basal medium and plant
growth regulator stocks.

Module III: Preparation of cotton plugs.

Module IV: Preparation and sterilization of explants and inoculation of explants on culture media.

Module V: Embryo Culture. To isolate embryos of Cicer aertinum and perform in vitro culture.

Module VI: Isolation of protoplasts by mechanical method.

Module VII: Isolation of protoplasts by enzymatic method.

Module VIII: Preparation of artificial seeds.

Module IX: Plant regeneration from callus or plant tissue.

Module X: Cell suspension culture.

70
BIOETHICS, BIOSAFETY & IPR

Course Code: EBT 501 Credit Units: 04

Unit I

Jurisprudential definition and concept of property, rights, duties and their correlation. History and evolution
of IPR- like patent design and copy right, Indian patent act 1970 (amendment 2000), international
convention in IPR, major changes in Indian patent system as post TRIPS effects:

(i) Obtaining patent (ii) Geographical Indication.

Unit II

Distinction among various forms of IPR, Requirement of a patentable novelty, invention step and prior art
and state of art, procedure.

Unit III

Rights/protection, infringement or violation, remedies against infringement civil and criminal.

Unit IV

Detailed information on patenting biological products, Biodiversity, Budapest treaty, Appropriate case
studies.

Unit V

Biosafety and Bioethical issues in Biotechnology

Recommended Books:

1. Patent Strategy For Researches & Research Manegers- Knight, Wiley Publications.
2. Agriculture & Intellectual & Property Rights, V. Santaniello& R E Evenson, University Press.
3. Intellectual Property Protection & Sustainable Development, Phillipe Cullet,
LdexixNexis Butterworths.
4. Biotechnology & Safety Assessment, Thomas, Ane/Rout Publishers.
5. Biotechnology in Comparative Perspective, Fuchs, Ane/Rout Publishers.

71
ENZYMOLOGY AND ENZYME TECHNOLOGY

Course Code: EBT 502 Credit Units: 04UNIT

UNIT I: Enzymes

Introduction and scope, Nomenclature, Mechanism of Catalysis.

UNIT II: Enzyme Kinetics

Single substrate steady state kinetics; MichaelisMenten equation, Linear plots, King-Altmans method;

Inhibitors and activators; Multisubstrate systems; ping-pong mechanism, Alberty equation, Sigmoidal

kinetics and Allosteric enzymes.

UNIT III

Immobilization of Enzymes; Advantages, Carriers, adsorption, covalent coupling, cross-linking and

entrapment methods, Micro-environmental effects.

UNIT IV: Enzyme reactors

Reactors for batch/continuous enzymatic processing, choice of reactor type; idealized enzyme reactor

systems, Mass transfer in enzyme reactors: Steady state analysis of mass transfer and biochemical reaction

in enzyme reaction.

UNIT V: Bioprocess Design

Physical parameters, reactor operational stability, Immobilized cells.

UNIT VI: Challenges and future trends

Enzyme catalysis in organic media; catalytic antibodies and non protein biomolecules as catalysts,

biocatalysts from extreme thermophilic and hyper thermophilicArchae and Bacteria.

Text & References:

Text:

Biotechnological Innovations in Chemical Synthesis, R.C.B. Currell, V.D. Mieras, Biotol Partners Staff,

Butterworth Heinemann.

Enzyme Technology, M.F. Chaplin and C. Bucke, Cambridge University Press.

72
Enzymes: A Practical Introduction to Structure, Mechanism and Data Analysis, R.A. Copeland, John

Wiley and Sons Inc.

References:

Enzymes Biochemistry, Biotechnology, Clinical Chemistry, Trevor Palner.

Enzyme Kinetics: Behaviour and Analysis of Rapid Equilibrium and Steady State Enzyme Systems, I.H.

Segel, Wiley-Interscience.

Industrial Enzymes & their applications, H. Uhlig, John Wiley and Sons Inc.

73
IMMUNOLOGY AND IMMUNOTECHNOLOGY

Course Code: EBT 503 Credit Units: 04

Course Contents:

Unit I: Introduction

Overview of Immune system: History and scope of Immunology, Types of immunity: innate, acquired,
Comparative immunity. Immune dysfunction and its consequences.

Unit II: Cells and Organs of Immune system

Cells of the immune system lymphoid cells: B, T and NK cells, Primary lymphoid organs, Secondary
lymphoid organs-lymph nodes, spleen mucosal associated lymphoid tissues.

Unit III: Generation of B-cell and T-cell response

Generation of B-cell and T- cell response: Antigens: Immunogenicity vs. antigenicity Epitopes (properties
of B-cell and T-cell epitopes)

Unit IV: Immunoglobulin Structure and Function

Basic and fine structure of immunoglobulin: light chains, heavy chains and sequences Antigen determinants
on Immunoglobulin: Isotopic, allotypic, Idiotypic; Immunoglobulin Super family; Immunoglobulin classes
and Biological activity: Immunoglobulin mediated Effectors functions optimization: Activation of
complement; Antibody dependent cell Mediated cytotoxicity. Clinical focus: Passive antibody therapy (IgG,
IgM, IgA, IgE and IgD), hypersensitivity and immunological disorder.

Unit V: Organization and Expression of Immunoglobulin Genes

Variable region gene rearrangements; Mechanism of variable region DNS rearrangements: Generations of
antibody diversity; Class switching among constant regions genes; Antibody and genes and antibody
engineering.

Unit VI: Antigen Antibody Interactions

Strength of antigen and antibody interactions: Antibody affinity, antibody avidity Cross-reactivity;
Precipitation reactions, agglutination reactions (immunodiffusion and Immuno-electrophoretic technique);
Radioimmunoassay; Enzyme linked Immunosorbant Assay (ELISA); Western Blotting; Immuno
precipitation; Immunofluorenscence; Flow cytometery and Fluorescence.

74
Unit VII: MHC-Major Histocompatibility complexes

MHC molecules and genes; Antigen processing and presentation, activation of B and T-Lymphocytes,
cytokines and their role in immune regulation, T cell regulation and MHC Restriction, immunological
tolerance.

Unit VIII: Immune System in Health and Disease

Immune response to infectious disease (AIDS, Malaria, Kala azar & TB); Vaccines (whole Organism,
purified macromolecules, recombinant vaccine, synthetic polypeptide etc. Autoimmunity; Hypersensitivity;
Transplantation immunology: graft rejections, graft vs. host Response; Cancer and immune system.

Text & References:

Text:

Basic Immunology, A.K. Abbas and A.H. Lichtman, Saunders W.B. Company

Basic Immunology, A.K. Abbas and A.H. Lichtman, Saunders W.B. Company

References:

Fundamentals of Immunology, W. Paul, Lippincott Williams and Wilkins

Immunology, W.L. Anderson, Fence Creek Publishing (Blackwell).

Immunology: A Short Course, E. Benjamin, R. Coico and G. Sunshine, Wiley-Leiss Inc.

Immunology, Roitt, Mosby Yearbook Inc.

Kuby Immunology, R.A. Goldsby, T.J. Kindt, and B.A. Osborne, Free

75
BIOPROCESS TECHNOLOGY

Course Code: EBT 504 Credit Units: 04

Course Contents:

Unit I

Advantage of bioprocess over chemical process.Basic principle in bioprocess technology.Media

formulation, Cell culture techniques; Inoculum development and aseptic transfers. Different types of pumps,

valves, and line materials, piping conventions etc. used in Biochemical Process.

Unit II

Process technology for the production of primary metabolites, eg.biomass, ethanol, acetone-butanol, citric

acid, amino acids, polysaccharides and plastics.

Ethanol: production by batch, continuous and cell recycle adopted by various technologies practiced in

Indian distilleries using molasses and grains. Computation of fermentation efficiency, distillation efficiency

and overall efficiency of ethanol production, recovery, uses, glucose effect etc.Power alcohol definition,

uses, merits and demerits of various technologies for its production.

Amino Acid: Genetic Control of metabolic pathway.

Lysine: Indirect and direct fermentation mechanism of ph of metabolic block in accumulation of L- lysine

by inhibition and repression mechanism.

Biomass: Bakers and distillers yeast production using various raw materials, bios factors for growth,

Crabtree effect, harvesting, different forms and uses. What are mushroom, different forms of common

mushroom production from agro based raw materials and uses. Biofertilizers, biocompost and Biopesticides.

Unit III

Production of secondary metabolites penicillin, cephalosporins, streptomycin, tetracycline etc.

Metabolites from plant and animal cell culture

Penicillin: Classification, various penicillin as precursor and R side chain, penicillinase, 6-APA,

penicillin production, harvest and recovery, uses of various forms etc.


76
Streptomycin: chemical structure, production, harvest and recovery, use by-product of streptomycin

fermentation etc.

Tetracycline: chemical structure, production, harvest and recovery, use by-product of tetracycline

fermentation etc.

Unit IV

Microbial production of industrial enzymes glucose isomerase, penicillin acylase, cellulase, amylase,

lipase, protease etc.

Text & References:

Biochemical Engineering- Kinetics, Mass Transport, Reactors and Gene Expression, W F


Weith, John Wiley and Sons Inc.

Biochemical Engineering, S Aiba, A E Humphery and N F Millis, University of Tokyo Press

Bioprocess Engineering Basic Concepts, M.L. Shuler and F. Kargi, Prentice Hall

Bioprocess Engineering, B.K. Lydersen, K.L. Nelson, B.K. Lyderson and N. DElia, John Wiley and

Sons Inc.

Bioprocess Engineering Principles, P Doran, Academic Press

Biotechnology. A Textbook of Industrial Microbiology, W. Crueger and a. Crueger,


Sinauer Associates.

Principles of Fermentation Technology, P.F. Stanbury and A. Whitaker, Pergamon Press

Process Engineering in Biotechnolgy, A T Jackson , Prentice Hall

77
BIOMEDICAL INSTRUMENTATION

Course Code: EBT 505 Credit Units: 04

Unit I

Introduction to biomedical instrumentation, Basic transducer principles, Sources of bioelectric


Potentials, Electrodes.

Unit II

The Cardiovascular system, Cardiovascular measurements, Patient care and monitoring, Measurements
in the respiratory system.

Unit III

Noninvasive diagnostic instrumentation, the nervous system, Instrumentation for sensory measurements and
the study of behaviours, Biotelemetry.

Unit IV

Instrumentation for the clinical laboratory, X-ray and radioisotope instrumentation.

Unit V

The computer in biomedical instrumentation, Electrical safety of medical equipment.

Recommended books:

1. Biomedical Instrumentation and Measurement by Leslie Cromwell, Fred J.


Weibell, Erich A. Pfeiffer
2. Biomedical Instrumentation: Technology and Applications by Raghbir Singh
3. Medical Instrumentation for Health Care by Leslie Cromwell
4. Analysis and Application of Analog Electronic Circuits to Biomedical Instrumentation
by Robert B. Northrop
5. Introduction to Bioinstrumentation: With Biological, Environmental, and Medical

78
INDUSTRIAL COMMUNICATION-V

Course Code: ASH 504 Credit Units: 02

Objective The Module provide the ways and means of enhancing innovative ideas and providing
solutions and enhance employability.

Unit -1: Soft Skills & hard skills


Introduction
Soft skills & Hard skills difference
What is Mentoring
Soft skills at Work place

Unit -2: Resume Designing


What is a Resume?
Types of Resumes
Writing of resumes

Unit 3: Interview Preparation


Interview tips
Common Interview Questions
Enhancing employability
Training & Grooming

Unit -4: English Literature


Nectar in Sieve Kamala Markandaya (Novel )
Purdah - Silvia Plath (Modern English Poetry)
Hope is the thing with feathers Dickenson (American Literature)

79
ENZYMOLOGY AND ENZYME TECHNOLOGY LAB
Course Code: EBT 522 Credit Units: 01

Course Contents:

List of experiments:

Module I: Isolation of enzymes from plant and microbial sources.

Module II: Enzyme assay; activity and specific activity determination of amylase, nitrate

reductase, cellulase, protease.

Module III: Purification of Enzyme by ammonium sulphate fractionation.

Module IV: Enzyme Kinetics: Effect of varying substrate concentration on enzyme activity,

determination of Michaelis-Mentenconstant (Km) and Maximum Velocity (Vmax.) using

Lineweaver-Burk plot.

Module V: Effect of Temperature and pH on enzyme activity.

Module VI: Enzyme immobilization.

80
IMMUNOLOGY AND IMMUNOTECHNOLOGY LAB

Course Code: EBT 523 Credit Units: 01

List of experiments:

Module I: Blood film preparation and identification of cells.

Module II: Identification of blood group.

Module III: Isolation of serum.

Module V: WIDAL test

Module VI: Radial Immuno Diffusion Test

Module VII: Ouchterlony Double diffusion Test

Module VIII: Immuno histo chemistry.

81
BIOPROCESS TECHNOLOGY LAB
Course Code: EBT 524 Credit Units: 01

List of experiments:

Module I: Isolation of industrially important microorganisms for microbial processes.

Module II: Determination of Thermal Death Point and Thermal death time of microorganisms for design of

a sterilizer

Module III: Determination of growth curve of a supplied microorganism and also determines substrate

degradation profile and to compute specific growth rate and growth yield from the data

obtained.

Module IV: Comparative studies of ethanol production using different substrates.

Module V: Isolation and screening of industrially important microorganism (Bacillus subtilis) from soil

sample.

Module VI: Production of l-Asparaginase by Bacillus subtilis and estimation of protein concentration by

ninhydrin test.

Module VI: Production and estimation of alkaline protease

Module VIII: Use of alginate for cell immobilization

82
DOWN STREAM PROCESSING

Course Code: EBT 601 Credit Unit 04

Unit I

An overview of Bio separation. Role of Downstream Processing in Biotechnology. Problems and


requiremen of bioproduct purification.Cost cutting strategies.

Unit II
Primary separation and Recovery Process: Cell, Disruption methods(Physical,chemical and Enzymatic) for
intracellular prducts.Removal of insoluble, biomass. Separation techniques Flocculation, Sedimentation,
Centrifugation and Filtration..

Unit III

Product Isolation - Extraction and Adsorption methods, Solid- Liquid , Liquid -liquid Separation,
Distillation.Enrichment operation-Membrane based Separation -micro and ultrafiltration ( Theory, Design
and Configuration of the Equipment), Precipitation methods Ammonium, Sulphate, organic solvents, High
molecular weight polymers.

Unit IV

Product Purification: Electrophoresis and Chromatography principle for product purification, Different
Electrophoresis technique Isoelectric, focussing ,chromatographic technique with special reference to ion-
exchange ,affinity, GLC, HPLC.

Unit V
Product Polishing: Crystallization, Drying. A Few case studies: Citric acid, Glutamic acid, Penicillin
G,Extracellular Enzymes, Intracellular enzymes, Antibodies.

Recommended Books:
1. Comprehensive biotechnology- Murray Moo-Young, Vol. II-latest ed., Pergan Publishers.
2. H. J. Rehm and G. Reed, Biotechnology- Vol. 3, 4, 5, Verlag Publishers
3. Stanbury&Whitteker, Principles Of Fermentation Technology, Pergamon Press
4. A Biologists Guide to Principles & Techniques of Practical Biochemistry- Wilson and
Golding, Cambridge University Press
5. Humphrey, Aiba& Miller, Biochemical Engg., Academic Press.
6. Biochemical Engg. -Bailly&Ollis, Academic Press.
7. Product Recovery in Bioprocess Technology, Heinemann, Butterworth Publication.
8. Electrophoresis in Practice, Westermeier- Wiley Publications.
9. Handbook of Analytical techniques-H. Gunzler, Wiley Publications.
10. International Methods of Analysis- Willard et al., CBS Publication.
11. Bioseparation Techniques, Sivasankar, Prentice-Hall of India, 2004.
12. Bioseparations (Principles & techniques), B. Sivasankar, Prentice-Hall of India, 2005.
13. Citric Acid Biotechnology, Linden, Ane/Rout Publishers.

83
DATABASE MANAGEMENT SYSTEMS

Course Code: EBT 602 Credit Units: 04

Course Objective:

It enables the students to access biological information networks and databases in order to understand the
different techniques of biotechnology to build detection systems especially in the prevention and treatment
of human diseases.
Course Contents:

Unit I: Overview and historical perspective


File systems vs. DBMS, advantages of DBMS;

Unit II: Describing and storing data in DBMS


Levels of abstraction and data independence; Data models and their comparison; Entity relationship model -
concepts, design, keys and features; Relational model -introduction, structure of the relational databases,
integrity constraints, Relational algebra and calculus -selection and projection, set operations, renaming,
Joins, Division etc.

Unit III: SQL and Perl

Unit IV: Database design

Functional dependencies, Normal forms; Concurrency control and database discovery -concept of
transaction: atomicity, consistency, isolation and durability, transactions and schedules, concurrent
execution of transactions, Lock based concurrency control, Database recovery
Unit V: Current trends

Distributed databases and multimedia databases;

Unit VI: Data warehousing and Data MiningText& References:

Text:
Data Mining: Concept and techniques, J. Han and M. Kamber, Morgan Kaufman.
Data Mining, A.K. Pujari, Sangam Books Ltd.
Database Management, P.C. Desai.
Introduction to Database Systems, C.J. Date, Addison Wesley Publishing.

References:
Principles of Database and Knowledge Based systems, J.D. Ullman, Computer Science Press.
The Data Warehouse Lifecycle Toolkit, John Wiley and Sons Inc.

84
GENETIC ENGINEERING

Course Code: EBT 603 Credit Unit 04

Unit-I: Introduction, Milestones in Genetic Engineering

Unit-II: Molecular tools and their applications: DNA & RNA modifying enzymes: Restriction enzymes and
other endonucleases, Exonucleases, Polymerases, Kinases, Methylases and Ligases.

Unit-III: Molecular Techniques: Restriction analysis of DNA, Restriction map, electrophoretic techniques
for nucleic acid protein analyses, DNA sequencing, Chemical synthesis of oligonucleotides, Southern,
Northern and Western blotting techniques.

Unit-IV: Different vectors for molecular cloning: Plasmids, Bacteriophages, Phagemids, Cosmids; YAC
and BAC, Transformation Techniques

Unit-V: Construction of genomic and cDNA libraries; Gene specific probes; Screening strategies for
isolation of genes.

Unit-VI: Alternative strategies of gene cloning: PCR techniques and their applications, introduction to two
and three hybrid systems, nucleic acids microarrays.

Unit-VII: Random and site-directed mutagenesis, Expression strategies for heterologous genes: Expression
of recombinant proteins in Bacteria, Yeast, Insect cells, Mammalian and Plant cells, in vitro transcription
and translation.

Suggested Readings / Books:

1. Principles of Gene Manipulation and Genomics (7th edition), by S.B. Promorose and R. M. Twyman,
Blackwell Publishing (2006)

2. Gene Cloning and DNA Analysis: An Introduction (6th edition), by T A Brown, Wiley Blackwell
Publications.

3. Recombinant DNA by Watson.J.D. et al, 1993, Scientific American Books, New York.

4. Biotechnology, U. Satyanarayana, Books and Allied (P) Ltd., Kolkata (2005)


85
MOLECULAR BIOLOGY

UNIT I: DNA Replication and repair


Mechanism of Prokaryotic and Eukaryotic DNA replication, Enzymes and accessory proteins involved
in DNA replication, DNA repair Mechanism.

UNIT II: Gene Expression


Prokaryotic transcription, Eukaryotic transcription, RNA polymerase, General and specific transcription
factors, Regulatory elements. Modifications in RNA: 5'-cap formation, transcription termination, 3 '-
end processing and polyadenylation, Splicing, Editing, Nuclear export of mRNA and mRNA stability.

UNIT III: Translation


Prokaryotic and Eukaryotic translation, the translation Machinery; Mechanisms of initiation, elongation
and termination, regulation of translation, co-and post-translational modifications of proteins.

UNIT IV: Regulation of Gene Expression in prokaryotic and eukaryotic systems


Lac operon, Ara operan, regulation in Eukaryotes

Unit V
Bioethical issues related to animal biotechnology.

Text & References:


Text:
Concepts of Genetics, W.S. Klug, and M.R. Cummings 2004, Pearson Education
Genome, T.A. Brown, John Willey & Sons Inc.
Molecular Biology of the Cell. B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson,
Garland Publishing
Gene VIII, Benjamin Lewin 2005, Oxford University Press
References:
Molecular Cell Biology, H. Lodish, A.Berk, S. Zipursky, P Matsundaira, D.Baltimore and J.E. Barnell,
W.H.Freeman and Company.
Molecular Cloning: A Laboratory Manual ( 3-Vilcume set), J. Sambrook, E.F. Fritsch and T. Maniatis,
Cold spring Harbor Laboratory Press.

86
STATISTICS FOR BIOLOGY

Course Code: EBT 605 Credit Units: 04

Course Contents:

Unit I

Statistics and Biostatistics: Preliminary concepts.Measures of Central Tendency: Mean, Median,

ModeMeasures of Dispersion: Range, Standard deviation, Variance

Unit II

Probability: Random Experiments, Trial and Event, Sample Space, Mutually Exclusive or Disjoint Events,

Mutually Exhaustive Events, Equally Probable Events, Complementary Event, Classical definition of

Probability, Statistical definition of Probability, Axiomatic definition of Probability, Addition theorem,

Multiplication theorem, Conditional Probability, Bayes Theorem. Expectation.

Unit III: Continuous Distribution

Normal Distribution, Properties of Normal distribution

Unit IV: Correlation

Bivariate distribution Correlation, Types of Correlation, Simple Correlation Coefficient for ungrouped data,

Properties and Interpretation of Correlation Coefficient, Coefficient of determination, Scatter diagram,

Standard Error, Probable error of Correlation Coefficient. Rank correlation, Some examples.

Unit V: Regression

Definition, Regression lines and Regression Coefficients, Properties of Regression Coefficients, Some

examples. Method of least square: Fitting of straight line

87
Unit VI: Introduction to the following Statistical terms

Parameter, Statistic, Null hypothesis, Alternative hypothesis, Critical region, Type1 Error, Type 11 Error,

Level of significance, P-value and its applications. Test of Significance for Small samples: One sample t-

test, Paired t-test, Degrees of freedom for t-test, F test for equality of Population variances, Degrees of

freedom for F-test. Test of Significance for Large samples: Normal test for sample mean and population

mean, Normal test for two sample means, Chi-square Test: Test of goodness of fit, Test of Independence of

attributes, Degrees of freedom for Chisquare test, Coefficient of contingency, Yates correction for

continuity. Analysis of Variance: One way and Two way (only Examples)

Text & References:

Text:

Introduction to Biostatistics, Ronald N. Forthfer and Eun Sun Lee, Publisher: Elsevier.

Statistical Methodology, S.P. Gupta, Publisher: S. Chand & Co.

Fundamentals of Statistics, S.C. Gupta. Publisher: S.Chand& Co.

References:

Biostatistics: A manual of Statistical Methodology for use in Health, Nutrition and Anthropology, K.

VisweswaraRao. Publisher: Jaypee Brothers Biostatistics: A foundation for analysis in the


Health Sciences,

W.W. Daniel, Publisher: John Wiley and Son

Fundamentals of Mathematical Statistics, S.C. Gupta and V.K. Kapoor, Publisher: S.Chand&
Co. Statistical Analysis, Kaushal, T.L. Publisher: Kalyani Publishers

Statistical Methods, Potri, D. Kalyani Publishers.

Mathematical Statistics, H.C. Saxena, and V.K. Kapoor: S. Chand & Company

Biostatistics, P.N. Arora and P.K. Malhan, Publisher: Himalaya Publishing House.

88
INDUSTRIAL COMMUNICATION-VI

Course Code: ASH 604 Credit Units: 02

Objective This Module is a combination of Technical paper writing and literature features

Unit -1: Reports


Types of Reports
Significance of Reports
Structure & Style
Report writing
Exercise

Unit 2: Proposals & Technical Paper


Types of proposals
Writing of proposals
Technical paper

Unit-3: Dissertation & Thesis


Abstract
Synopsis
Dissertation
Thesis
Features of Thesis
Methods & Writing

Unit -4: English literature


Arms and the man - George Bernard Shaw (Drama)
All things will die Lord Alfred Tennyson (Poem)
The Flower - Lord Alfred Tennyson (Poem)

89
DOWNSTREAM PROCESSING LAB

Course Code: EBT 621 Credit Units: 01


Course Objective:

The laboratory will help the students to extract different bioproducts during their characterization since

any of these products may be of biotechnological importance. They can be exploited in exploring the

future biotechnology.

Course Contents:

Module I

Conventional filtration and membrane based filtration

Module II

Protein precipitation and recovery

Module III

Aqueous two-phase separation

Module IV

Ion exchange chromatography

Module V

Gel Permeation chromatography

Module VI

Electrophoresis.

90
DATABASE MANAGEMENT LAB

Course Code: EBT 622 Credit Units: 01


Course Contents:

Module I

Database creation using DDL and DML.

Module II

Defining the primary and secondary keys.

Module III

Implementation of selection, projection and joins (internal and external) with SQL and Perl.

Module IV

Normalization of databases with SQL and Perl

Module V

Implementation of transactions and schedules.

Module VI

Detection of association rules and knowledge recovery.

91
BIOTECHNOLOGY IN HEALTH CARE

Course Code: EBT 701 Credit Units: 04

Course Contents:

Unit I

Therapeutic Aspects of Biomacromolecules: Introduction, Endogenous pepticdes and proteins,


Modification of endogenous peptides and proteins Immune System: Overview, Antibody-mediated
response, Vaccines, Cell-mediated immune response, Cancer immunotherapy

Unit II

Oligonucleotides: Overview, Gene therapy, Antisense therapy, Ribozymes Oligosaccharides: Overview,


Oligosaccharide synthesis, Heparin, Glycoproteins, Polysaccharide bacterial vaccines, Approaches to
carbohydrate-based cancer vaccines.

Unit III

Radiological Agents: Radiosensitizers and Radioprotective agents Cardiovascular Drugs: Myocardial


infarction agents, Endogenous vasoactive peptides, Hematopoietic agents, Anticoagulants, anthrombotics
and hemostatics

Unit IV

Chemotherapeutic Agents: Synthetic antibacterial agents, Lactam antibiotics, Anthelminitic agents,


Anthelmintic agents, Antiamebic agents, Antiviral agents. Endocrine Drugs: Female sex hormones and
analogs, Agents affecting the immune Response

Unit V

Drug Targeting Organ-Specific Strategies: Basic concepts and novel advances, Brain-specific drug
targeting strategies, Pulmonary drug delivery, Cell specific drug delivery.

Text / Reference Books:

1. Pharmaceutical Chemistry by Christine M. Bladon. John Wiley & Sons, Ltd. (2002).

2. Burgers Medicinal Chemistry and Drug Discovery (5th edition) by Manfred E. Wolff. A Wiley & Sons,
Inc. (2000).

3. Drug Targeting Organ-Specific Strategies by Grietje Molema and Dirk K. F. Meijer. Wiley-VCH. (2002).

92
CHEMICAL ENGINEERING PRINCIPLES

Course Code: EBT 702 Credit Units: 04

Course Contents:

Unit I: Material and Energy Balances

Units and dimensions, Dimensional analysis; Simple problems on material balance calculations involving

unit.processes and reactive systems; Available electron balances. Basic energy concepts -enthalpy changes

in chemical/biochemical reactions and in non-reactive processes, Energy balance calculations, Use of Steam

tables; Heat of reaction and energy balance for microbial processes.

Unit II: Chemical reaction engineering

Kinetics of homogenous reactions: Concepts of reaction rate, order of reaction and molecularity, Analysis

of batch reactors for kinetic interpretation of data and isothermal reactor design for single and multiple

reactions, Design equations for CSTR and plug flow reactors.

Unit III: Instrumentation and process control

Principles of measurement: error, accuracy and sensitivity; Measurement of flow, pressure, temperature,

level, pH, viscosity and chemical composition. Basic concepts of feedback control, control loop and its

elements, Dynamic behaviour of first, second and higher order physical systems, controller hardware,

choice of controllers and settings. Introduction to advanced control systems: feed forward, cascade and ratio

control.

Text & References:


Text:
Basic Principles and Calculations in Chemical Engineering, D.M. Himmelblau, Prentice Hall
Basic Principles of Chemical Engineering, E.I. Shaheen, Houghton Mifflin

References:
Chemical Process Control, An introduction to Theory and Practice, G. Stephanopoulos, Prentice Hall Inc.
Chemical Reaction Engineering, O. Levenspiel, John Wiley and Sons Inc.
Elementary Principles of Chemical Processes, R.M. Felder and R.W. Rousseau, John Wiley and Sons Inc.
Fundamentals of Chemical Reaction Engineering, C.D. Holland and R.G. Anthony, Prentice Hall Inc.
Process Modelling, Simulation and Control for Chemical Engineers, W.L. Luyben, McGraw Hill

93
THERMODYNAMICS OF BIOLOGICAL SYSTEMS

Course Code: EBT 703 Credit Units: 04

Course Objective:

The main aim is to understand the basis of theories related to combustion and dissolution. It will help in the

study of heat evolution, control of dissipation and will suggest ways for solving the rate phenomena through

the dynamics of mass transfer applicable to biological process.

Course Contents:

Unit I

Energy, thermodynamics and living processes - an introduction

Unit II

Energetic processes in the biosphere: The ecosystem.

Unit III

Thermodynamics systems: equilibrium, activity coefficients and phase equilibrium functions of state, cyclic

processes, work, energy and metabolic heat; Mechanical equivalent of heat, energy as a function of state.

Unit IV: The laws of thermodynamics

Second law; Reversible vs. irreversible processes entropy, work; Combination of the first and second

law, Free energy, useful work and delta G. Entropy: Ideality and Molecular Cohesion, Probabilistic

nature of Entropy, Order and Disorder.

Unit V: Biological systems as open, non-equilibrium systems

Failure of classical thermodynamics in describing biological processes, Standard free energy changes

and equilibrium constants, direction and rate.

Unit VI: Chemical potential

Visualization of the potential velocity and steady flow; Ficks law and diffusion. Local Equilibria and

steady state: Energy vs. Power; Transducers in biological states, Constitutive equations, Dynamic efficiency

and Onsager (nonequilibrium thermodynamics), Prigogines principle, Spontaneous coupling and entropy

production.

94
Unit VII: Non-equilibrium thermodynamics

Reversible work, exact differentials and function of state, first and second law, The electrochemical

potential, External forces an steady state, Ficks Law, chemical reactions in the steady state, internal

entropy production, cells as non-equilibrium stationary states; Diffusion and membrane transport.

Thermodynamic analysis of oxidative photophospghorylation, stability of non-equilibrium stationary states,

ordering in time and space far from equilibrium, glycolytic oscillations, biological clocks, routes to chaos.

Text & References:

Bioenergetics, A.L. Lehninger, W.A. Benjamin Inc.

Biological Thermodynamics, D.T. Haynie, Cambridge University Press.

Biophysical Chemistry, C.R. Cantor and P.R. Schimmel, Freeman

Physical Chemistry: Principles and Applications in Biological Sciences, I. Tincoco, K.Sauer and

J.C. Wang, Prentice Hall College Division.

Physical Chemistry for the Chemical and Biological Sciences, R. Chang, University Science Books

Thermodynamics and Kinetics for the Biological Sciences, G.G. Hammes, John Wiley and Sons Inc

95
CURRENT TOPICS IN BIOTECHNOLOGY

Course Code: EBT 704 Credit Units: 04

Course Contents:

Unit I: Bioremediation

Introductory concept, Bioremediation principles, mechanism for Bioremediation of air, water and soil

pollutants, bioremediation examples, commercial application of bioremediation techniques, recombinant

DNA technology and bioremediation, bioremediation models, bioremediation software.

Unit II: Genetically modified organisms

Genetically modified food crops, food animals examples and mode of production, future goals in GM

food crops and animals, scientific evaluation of public concerns, legal requirements in production of GMO,

current trends and consumer acceptance.

Unit III: Molecular medicine

Gene mutation, point mutation, allele specific oligonucleotides, ARMS, oligonucleotide ligation,

disease diagnosis with linked genetic markers, fluorescently labeled DNA sequencing.

Unit IV: Nano-biotechnology

Introduction, definition, hybrid nanopracticles, smrat drug delivery, gene sensors, biomolecule

control, nanofluids, nanotechnology in medicine.

Unit V: Stem cells

Definition, properties, proliferation, culture of stem cells, medical applications of stem cells, ethical

and legal issues in use of stem cells.

Unit VI: Cancer Biology

Types of cancer, development, causes and properties of cancer, viruses (Hepatitis B & C, SV4U, polymo

marines, pupillomarines, adenosines, retrovineses ); retroviral oncogenes, proto-oncogenes, turmor

suppressor genes, recent advances in detection and treatment of cancer.

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Unit VII: Forensic Biotechnology

MLP, SLP technology, PCR technology in crime detection, STR and databases, mitochondrial DNA and Y

chromosome analysis in forensic science, DNA chip technology, role of molecular biology and

biotechnology incrime detection.

Unit VIII: Bio sensor

Biological reaction, amperometric biosensor, potentiometric biosensor, conductimetric bioseosors,

calorimetric biosensor, piezoelectric biosensor, whole-cell biosensor, immune sensors.

Text & References:

The Cell A molecular Approach, 3rd Edn, Geoffrey M. Cooper and Robert E. Hausman, ASM Press

Molecular Biology and Biotechnology, 4th Edn, J.M Walker and R. Rapley, Panima Books

Cell Biology, David. E. Sadava, Panima Books

Stem Cell Biology, Daniel Marshak, Richard L. Gardener and David Gottlieb, Cold Spring Harbour

Laboratory Press

Environmental Microbiology, 2nd Edition, Ian L. Pepper and Charles P. Gerba, Elsevier Pub.

Environmental Biotechnology Concepts and Application, Hans Joachim Jordening and Jesefwinter

Wiley VCH

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ENVIRONMENTAL BIOTECHNOLOGY

Course Code: EBT 801 Credit Units: 03

Introduction
Ecology and ecosystem.

Module II: Environmental pollution


Water, soil and air, noise and thermal pollution, their sources and effects.

Module III: Waste water (sewage and industrial effluents) treatments


Anaerobic and aerobic treatment, conventional and advanced treatment technology,
methanogenesis, methanogenic,acetogenic, and fermentative bacteria- technical process and
conditions, emerging biotechnological processes in waste - water treatment.

Module IV: Solid waste management


Landfills, composting, earth worm treatment, recycling and processing of organic residues.

Module V: Biodegradation
Biodegradation of xenobiotic compounds, organisms involved in degradation of chlorinated
hydrocarbons, substituted simple aromatic compounds, polyaromatic hydrocarbons, pesticides, surfactants
and microbial treatment of oil pollution

Module VI: Microbial leaching and mining


Microbial leaching and mining: Extraction of metals from ores; Recovery of metals from solutions;
Microbes in petroleum extraction; Microbial desulfurization of coal.

Module VII: Wasteland


Wasteland: Uses and management, bioremediation and biorestoration of contaminated lands.

Module VIII: Environmental genetics


Environmental genetics: degradative plasmids, release of genetically engineered microbes in environment.

Module IX: Hazardous wastes


Hazardous wastes: source management and safety.

99
PRINCIPLES OF MANAGEMENT AND

ENTREPRENEURSHIP DEVELOPMENT

Course Code: EBT 802 Credit Units: 03

Course Objective:

The Management and Entrepreneurship program is designed to prepare students for an exciting career in

todays competitive era. The course will equip students with the knowledge to cope up with the changing

environment because of the advent of technology and other influences. The course will also develop

required entrepreneurship skills in the students from a variety of disciplinary perspectives known to be

important for independent and corporate entrepreneurs.

Course Contents:

Module I

Principles and function of management, Planning and decision making, Line and staff

relationship, management by objective.

Module II

Formal and informal organization, Performance appraisal, Training and development.

Module III

Entrepreneurship and entrepreneurial process, Business plan, Form of ownership suitable for business.

Module IV

Entrepreneurial motivation and leadership, entrepreneurial competencies, entrepreneurial

development programme.

Text & References:

Text:

Essentials of Management, H. Koontz, H. Weihrich and C. ODonnell, McGraw-Hill/Irwin

David H Holt, Entrepreneurship : New Venture Creation

References:

The Practice ofs Management, P. Drucker, Harper Business.

100
MARKETING & MANAGEMENT OF BIOTECHNOLOGY

Course Code: EBT 803 Credit Units: 03

Course Objective:

1. Why there is a need to commercialize biotechnology. Discovery, market needs, development process,

success rates and costs etc.

2. Creating and marketing the image of the biotechnology Company. Art of negotiation & effective

communication.

3. Role of venture capitalism, business plan, selection of CEO and personnel, real estate for a biotech start-

up.

4. How to portray management and role of a biotechnology manager, technology decisionmaking, and

resource decision-making etc., Product marketing decision.

5. Role of Research & development University-industry technology transfer arrangements, how and why a

biotech company can benefit.

6. Positioning, power and importance of positioning of a company name and product, Workable marketing

and the strength of distribution.

7. Effective advertising and marketing. Opportunities international, marketing and lessons to be learned.

8. Indian and foreign prospective of biotechnology, and current challenges for thebiotechnology based

products.

Text / Reference Book:

1. Positioning by All Rise and Jack Trout (1986), Warner Books.

2. Biotechnology: The science & the business by V. Moser & R.E. Cape (1999) Harwood.

3. Latest review articles and papers on the subject.

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