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3 Making standart curve Before : As long the biggr As long the biggr
Glucose standart : concentration the concentration the
1 mL glucose 1 mL 1 mL glucose 1 mL glucose colorless soluion adsorbance will rising adsorbance will
[0,3 mg/ml] glucose [0,5 [0,7 mg/ml] [0,9 mg/ml] Aquades : colorless too and the graphic rising too and the
mg/ml] Cu-alkalis : light showed linier rising up graphic showed
poured into test tube blue solution linier rising up
Added 3 mL Cu alkalis Arsenomolibdat : 2Cu2+ + C6H12O6 + Y = 0,2057X +
Inserted into boiling water 15 minutes pale yellow solution 5OH- Cu2O (s) + 0,5332
Inserted into cooled water for 10 After : H2O (l) R2 = 0,9752
minutes
Glucose after diluted Cu+ + [AsMo12O40]3-
Added 2 drops arseno mollibdat Cu2+ +
Read adsorbance with
25 ml from 11,25 ml
get diluted solution [AsMo12O40]4-
spektrotonic 20 in wave 660 nm
0,3 mg/ml, 0,5
Adsorbancy
mg/ml, o,7 mg/ml,
0,9 mg/ml
Glucose + Cu-
alkalis : light blue
Glucose + cu-alkalis
+ heat
1. Solution 0,9 mg/ml
: Blue solution (+)
+ surrel precipitate
(++)
2. Solution 0,7 mg/ml
: Blue solution
(++) + surrel
precipitate (+)
3. Solution 0,5 mg/ml
: Blue solution
(+++) + surrel
precipitate (+)
4. Solution 0,9 mg/ml
: Blue solution
(++++) + surrel
precipitate (+)
After cooled +
arseno molibdat :
1. Solution 0,9 mg/ml
: Blue solution (+)
2. Solution 0,7 mg/ml
: Blue solution
(++)
3. Solution 0,5 mg/ml
: Blue solution
(+++)
4. Solution 0,3 mg/ml
: Blue solution
(++++)