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A. H. Stead et al., Analyst, 1982, 107, 11061168 and R. A. de Zeeuw et al., Thin-layer
Chromatographic Rf Values of Toxicologically Relevant Substances on Standardized Systems:
Report XVII of the DFG Commission for Clinical-Toxicological Analysis, 2nd Edn, VCH,
Weinheim, 1992.
System TA
Plates: Silica gel G, 250 m thick, dipped in, or sprayed with, 0.1 M potassium
hydroxide in methanol, and dried.
Mobile phase: Methanol:strong ammonia solution (100:1.5).
Reference compounds: Atropine Rf 18, Codeine Rf 33, Chlorprothixene Rf 56,
Diazepam Rf 75.
System TB
Plates: Silica gel G, 250 m thick, dipped in, or sprayed with, 0.1 M potassium
hydroxide in methanol, and dried.
Mobile phase: Cyclohexane:toluene:diethylamine (75:15:10).
Reference compounds: Codeine Rf 06, Desipramine Rf 20, Prazepam Rf 36,
Trimipramine Rf 62.
System TC
Plates: Silica gel G, 250 m thick, dipped in, or sprayed with, 0.1 M potassium
hydroxide in methanol, and dried.
Mobile phase: Chloroform:methanol (90:10).
Reference compounds: Desipramine Rf 11, Physostigmine Rf 36, Trimipramine
Rf 54, Lidocaine Rf 71.
System TL
Plates: Silica gel G, 250 m thick, dipped in, or sprayed with, 0.1 M potassium
hydroxide in methanol, and dried.
Mobile phase: Acetone.
Reference compounds: Amitriptyline Rf 15, Procaine Rf 30, Papaverine Rf 47,
Cinnarizine Rf 65.
System TAE
System TAF
Ninhydrin spray
Spray the plate with the reagent and then heat in an oven at 100 for 5 min. Violet or pink spots
are given by primary amines and yellow colours by secondary amines.
FPN reagent
Red or brown-red spots are given by phenothiazines and blue spots by dibenzazepines. This
reagent may be used to overspray a plate which has been previously sprayed with ninhydrin
spray.
Dragendorff spray
Yellow, orange, red-orange, or brown-orange spots are given by tertiary alkaloids. This reagent
may be used to overspray a plate which has been previously sprayed with ninhydrin spray and
FPN spray.
Mandelins reagent
This reagent is preferably poured onto the plate because of the danger of spraying concentrated
acid. Many different colours are given with a variety of drugs (see Chapter 19 and the Index of
Colour Tests).
Marquis reagent
This reagent is preferably poured onto the plate because of the danger of spraying concentrated
acid. Black or violet spots are given by alkaloids related to morphine. Many different colours are
given with a variety of drugs (see Chapter 19).
Rf values
Rf values for drugs in these systems will be found in drug monographs and in the Indexes of
Analytical Data in Volume 2; they are also included in the systems for specific groups of drugs
which follow.
A. H. Stead et al., Analyst, 1982, 107, 11061168 and R. A. de Zeeuw et al., Thin-layer
Chromatographic Rf Values of Toxicologically Relevant Substances on Standardized Systems:
Report XVII of the DFG Commission for Clinical-Toxicological Analysis, 2nd Edn, VCH,
Weinheim, 1992.
System TD
System TE
System TF
Plates: Silica gel G, 250 m thick.
Mobile phase: Ethyl acetate.
Reference compounds: Sulfathiazole Rf 20, Phenacetin Rf 38, Salicylamide Rf
55, Secobarbital Rf 68.
System TAD
Acidic drugs
Neutral drugs
Furfuraldehyde reagent
Violet to blue-black spots are given by some neutral compounds, e.g. carbamates.
Rf values
Rf values for drugs in these systems will be found in drug monographs and in the Indexes of
Analytical Data in Volume 2; they are also included in the systems for specific groups of drugs
which follow.
Note
It is worth noting that system TE can be used for acidic, neutral and basic drugs. Furthermore,
systems TC and TAD use the same mobile phase so that acidic, neutral and basic drugs can be run
in the same tank, although on separate plates. It should also be noted that the above systems for
basic nitrogenous drugs are also able to separate neutral drugs if the latter are present in the
sample or in the basic extract thereof.
Finally, the Index of Colour Tests lists colour reactions with TLC spray reagents for
approximately 250 compounds and may therefore serve as an indication of colour reactions
specific to certain classes of compounds.
General screening systems
The TLC systems listed below (Systems TAJ, TAK and TAL) were developed primarily by
Professor George Maylin, New York State Racing Wagering Board, Drug Testing Programme, as
well as System TAM listed under steroids.
System TAJ
System TAK
System TAL
Cupric chloride
Dragendorff spray
Fearon's reagent
Fluorescamine
Gibb's reagent
Iodine
Mandelin's reagent
Sodium nitrite
Ninhydrin