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Forensic Science International: Genetics 2 (2008) e71–e72


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Announcement of Population Data


16 STR data of a Greek population
P. Sánchez-Diz a,*, P.G. Menounos b, A. Carracedo a, I. Skitsa c
a
Institute of Legal Medicine, Genomics Medicine Group, University of Santiago de Compostela, Santiago de Compostela, Spain
b
Nursing Military Academy, Laboratory of Research, Athens, Greece
c
DNA Analysis Laboratory, Athens Legal Medicine Department, Ministry of Justice, Greece
Received 4 December 2007; received in revised form 22 January 2008; accepted 27 January 2008

Abstract
Allele frequencies for 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA,
TPOX, D18S51, D5S818, FGA, and SE33) were calculated for a sample of 300 unrelated individuals from Greece. No deviations from Hardy–
Weinberg equilibrium were observed for all loci. The combined power of discrimination (PD) and the combined power of exclusion (PE) for the 16
tested STR loci were 0.999999999 and 0.999999816, respectively. Population comparisons were carried out and low genetic distances were found
between our data and those previously published for other neighbouring European populations.
# 2008 Elsevier Ireland Ltd. All rights reserved.

Keywords: STRs; Population data; AmpFlSTR Identifiler; AmpFlSTR SEfiler; Greece; Greeks

Population: Blood samples were obtained from 300 Matching Probability) were calculated using PowerStats v1.2
unrelated healthy individuals living in Greece after informed (Promega Corporation, USA) software package.
consent. The samples were chosen randomly according to their Quality control: Proficiency testing of the GEP-ISFG WG
origin from Peloponnese, Crete, Western Greece islands and (http://www.gep-isfg.org) and GEDNAP blind trials (http://
mainland, Thrace, Macedonia, Central Greece, Aegean islands, www.gednap.de.vu).
Evia and Epirus. Hence, this is a representative sample of the Results: The whole genotype data set, allele frequencies and
general Greek population. genetic distances between the studied sample and other
Extraction: DNA was extracted from blood stains by European populations [2–10] are available as e-component.
Chelex method. Other remarks: For all STRs studied, no deviations from
PCR: According to the manufacturer’s conditions Hardy–Weinberg expectations were observed. The combined
(AmpFlSTR Identifiler PCR Amplification Kit, and AmpFlSTR power of exclusion (PE) and power of discrimination (PD) for
SEfiler PCR Amplification Kit, Applied Biosystems, Foster City, the 16 studied loci were 0.999999816 and 0.999999999,
CA, USA). respectively. The combined matching probability value was 1 in
Typing: PCR products were analysed by capillary electro- 2.78029  1019. Based on heterozygosity and polymorphic
phoresis in an ABI 310 Genetic analyzer (AB). Allele information content, SE33 may be considered as the most
assignment was carried out by comparison with reference informative loci. Allele frequencies at Greece sample were
sequenced ladders (AB). compared with data available for the same markers in other
Analysis of data: Arlequin software ver. 3.0 [1] was used to European populations [2–10]. In general, low genetic distance
calculate allele frequencies, population pairwise genetic values were obtained in all comparisons (see the e-component).
distances (FST), and also to assess departures from Hardy– After applying the Bonferroni correction for multiple tests,
Weinberg equilibrium. Statistical parameters of forensic population differentiation tests showed that Greece had
interest (Power of Discrimination, Power of Exclusion and significant differences with Podlasie in 5 out of 14 loci
(D5S818, D8S1179, D18S51, D21S11 and TH01), with
northern Poland in 1 out of 15 loci (D21S11), and with a
* Corresponding author at: Forensic Genetics Unit, Institute of Legal Med-
icine, Genomics Medicine Group, University of Santiago de Compostela, c/San
sample of Kosovo Albanians in 1 out of 15 loci (D18S51).
Francisco s/n, 15782 Santiago de Compostela, Spain. No inconsistencies were observed between genotypes in the
E-mail address: paulaiml@usc.es (P. Sánchez-Diz). loci shared by both kits.

1872-4973/$ – see front matter # 2008 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.fsigen.2008.01.002
e72 P. Sánchez-Diz et al. / Forensic Science International: Genetics 2 (2008) e71–e72

In summary, other STR databases of Greece are available in [4] Z. Szczerkowska, E. Kapińska, J. Wysocka, L. Cybulska, Northern Polish
population data and forensic usefulness of 15 autosomal STR loci,
the literature [11–15], however, here we report data previously
Forensic Sci. Int. 144 (2004) 69–71.
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widespread in all the country. Tasić, Allele frequencies of the 15 AmpF lSTR Identifiler loci in the
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