QUICK REFERENCE

Workflows for Detection of E. coli in Food Samples

Pub. No. MAN0009419 Rev. C.0
Note: Validated workflows include enrichment, sample preparation, detection, and confirmation methods described in the specified user guides.
Refer to the appropriate publication for the specific matrices included in each validated workflow.

NF VALIDATION™-certified workflows (AFNOR™) for PCR detection

Workflow overview
Species E. coli O157:H7 E. coli O157:H7 E. coli O157:H7
NF VALIDATION™ Raw beef meat and raw produce Raw beef meat and raw produce Raw beef meat
categories
Nucleic acid (NA) isolation Applied Biosystems™ PrepSEQ™ Nucleic Applied Biosystems™ PrepSEQ™ Rapid Thermo Scientific™ SureTect™ E. coli
(sample prep) Acid Extraction Kit (Cat. Nos. 4480466, Spin Sample Preparation Kit (Cat. Nos. O157:H7 PCR Assay (Cat. No. PT0400A)
4428176) 4407760, 4426714, 4413269, 4426715)
NA isolation workflow Automated Spin column Direct lysis
PCR detection Applied Biosystems™ MicroSEQ™ E. coli Applied Biosystems™ MicroSEQ™ E. coli Thermo Scientific™ SureTect™ E. coli
O157:H7 Detection Kit (Cat. No. 4427409) O157:H7 Detection Kit (Cat. No. 4427409) O157:H7 PCR Assay (Cat. No. PT0400A)
User guide (Pub. No.) 4426513: Sample enrichment and NA 4426519: Sample enrichment and NA D12162
isolation isolation
4426511: PCR[1] and confirmation 4426511: PCR[1] and confirmation
[1] Refer to the specified user guide for the real-time PCR instruments and analysis software included in the NF VALIDATION™-certified workflow.

AOAC™Performance Tested Methods - certified workflows for PCR detection

SM

Workflow overview
Species E. coli O157:H7 E. coli O157:H7 E. coli O157:H7 and non- E. coli O157:H7 and non- E. coli O157:H7
O157 STECs[1] O157 STECs[1]
Performance Meat products, leafy green Meat products, leafy green Raw beef Raw beef Raw beef, leafy green
Tested Methods products, and juices products, and juices products, and juices
SM

matrices
Nucleic acid (NA) Applied Biosystems™ Applied Biosystems™ Applied Biosystems™ Applied Biosystems™ Thermo Scientific™
isolation PrepSEQ™ Nucleic Acid PrepSEQ™ Rapid Spin PrepSEQ™ Nucleic Acid PrepSEQ™ Rapid Spin SureTect™ E. coli O157:H7
(sample prep) Extraction Kit Sample Preparation Kit Extraction Kit Sample Preparation Kit – PCR Assay (Cat. No.
(Cat. Nos. 4480466, (Cat. Nos. 4407760, (Cat. Nos. 4480466, Extra Clean with PT0400A)
4428176) 4426714, 4413269, 4428176) Proteinase K (Cat. Nos.
4426715) 4426715)
NA isolation Automated Spin column Automated Spin column Direct lysis
workflow
PCR detection Applied Biosystems™ Applied Biosystems™ Applied Biosystems™ Applied Biosystems™ Thermo Scientific™
MicroSEQ™ E. coli O157:H7 MicroSEQ™ E. coli O157:H7 RapidFinder™ STEC RapidFinder™ STEC SureTect™ E. coli O157:H7
Detection Kit (Cat. No. Detection Kit Screening Assay (Cat. No. Screening Assay (Cat. No. PCR Assay (Cat. No.
4427409) (Cat. No. 4427409) 4476886) 4476886) PT0400A)
Applied Biosystems™ Applied Biosystems™
RapidFinder™ STEC RapidFinder™ STEC
Confirmation Assay Confirmation Assay
(Cat. No. 4476901) (Cat. No. 4476901)
User guide (Pub. 4426513: Sample 4426519: Sample 4477013 MAN0015934 D12162
No.) enrichment and NA enrichment and NA
isolation isolation
4426511: PCR[2] and 4426511: PCR[2] and
confirmation confirmation
[1] Serogroups O26, O45, O103, O111, O121, and O145.
[2] Refer to the specified user guide for the real-time PCR instruments and analysis software included in the Performance Tested Methods -certified workflow.
SM

For testing of Food and Environmental samples only.

AOAC™ Performance Tested Methods -certified workflows for selective agar detection
SM

Workflow overview
Species E. coli O157:H7 E. coli O157:H7 E. coli O157:H7
Performance Tested Raw ground beef Raw ground beef Raw ground beef
Methods matrices
SM

Sample preparation
Sample preparation
workflow
Detection
User guide (Pub. No.)
Applied Biosystems™ Pathatrix™ E. coli Applied Biosystems™ Pathatrix™ 5- Applied Biosystems™ Pathatrix™ 10-
O157:H7 Kit (Cat. No. APE50SD; individual Pooling E. coli O157:H7 Kit Pooling E. coli O157:H7 Kit
samples) (Cat. No. APE250SDP; pooled samples) (Cat. No. APE500SDP; pooled samples)
Immunomagnetic separation Immunomagnetic separation Immunomagnetic separation
Selective agar Selective agar Selective agar
MAN0007092 MAN0007090 MAN0007091

The information in this guide is subject to change without notice.

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6 January 2017