Organism Selected- Nostoc punctiforme ATCC 29133

3. Number of chromosomes- 1
Number of Plasmids – 5
Genome Size - 9.05919 Mb
rRNA – 12
tRNA - 74

(https://www.ncbi.nlm.nih.gov/genome/1050)

4. 16S rRNA (or rDNA) sequence

taattctgt attggcgcaa ttccttgcca gataatgtcc acaaaatttt ggataaatgc
tgatacttct gtaccacaat cttcagagtt taagtaactt tttatggaaa taacgtctgt
ttttatactt ttttgagacg aaatctgctc taaaaaaaca tagttcatca acgatcccag
taaaatatga gccactgtct ggggatcgca agggcgaagg cgattttggt tgatttcata
ctctagaaaa gcggtaagtg ctttgaaatc ttgtatgggt cttggttcat tacctccaag
ttctggaaac gcattgcctc gcgatcgcag catcacgatc tgaggcagca cctcacgata
aaattccata atctggaggc aaatattgat taggttttct ttgagatcgc ctttaccact
aagagattcc aactcgctca cccacacggg tttttctgga attcccatcg ccgcaaaaaa
taattcttct ttggttgaga atcgcttgaa aattgaagct tcagaaatac ctgcctgttg
agcaatttct aaggttgaag caccaaatcc ttgttgcagg aaaacttgac gagccgcttc
taatatctgc tgattggtga ttttgggtat acgagccat

5. The 16S rRNA gene is used for phylogenetic studies as it is highly conserved between different species
of bacteria and archaea. 16S rRNA gene can be used as a reliable molecular clock because 16S rRNA
sequences from distantly related bacterial lineages are shown to have similar functionalities Some
thermophilic archaea contain 16S rRNA gene introns that are located in highly conserved regions and
can impact the annealing of "universal" primers.
Source: Wikipedia
6.

https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_4325025

7.Codon Usage Table-

UUU 28.6( 367) UCU 17.7( 227) UAU 18.6( 238) UGU 5.9( 75)
UUC 9.1( 116) UCC 5.8( 74) UAC 10.6( 136) UGC 3.8( 49)
UUA 33.8( 433) UCA 10.5( 135) UAA 1.7( 22) UGA 0.2( 2)
UUG 23.5( 301) UCG 5.5( 70) UAG 1.2( 15) UGG 11.7( 150)

CUU 11.5( 148) CCU 14.9( 191) CAU 10.8( 139) CGU 11.9( 152)
CUC 10.6( 136) CCC 10.6( 136) CAC 7.4( 95) CGC 11.1( 142)
CUA 14.0( 179) CCA 18.0( 231) CAA 39.1( 501) CGA 8.9( 114)
CUG 14.0( 179) CCG 5.5( 70) CAG 16.4( 210) CGG 7.6( 97)

AUU 36.8( 472) ACU 20.3( 260) AAU 30.0( 385) AGU 15.4( 197)
AUC 17.4( 223) ACC 10.8( 138) AAC 14.5( 186) AGC 10.5( 134)
AUA 10.3( 132) ACA 15.8( 202) AAA 40.5( 519) AGA 9.3( 119)
AUG 20.3( 260) ACG 5.5( 70) AAG 14.4( 184) AGG 3.2( 41)

GUU 21.5( 275) GCU 29.7( 380) GAU 35.7( 458) GGU 24.3( 312)
GUC 10.5( 134) GCC 16.1( 206) GAC 13.1( 168) GGC 13.6( 174)
GUA 18.0( 231) GCA 24.4( 313) GAA 50.8( 651) GGA 15.1( 194)
GUG 13.9( 178) GCG 11.9( 153) GAG 16.3( 209) GGG 10.0( 128)

Coding GC 42.61% 1st letter GC 53.71% 2nd letter GC 38.51% 3rd letter GC 35.60%

8. Total Protein- 6794

Total Hypothetical Protein - 2614
Unknown Protein- 22
Protein with assigned function- 4158
 Hypothetical Proteins constitute 38.47% and Protein with assigned functions constitute 61.20% of the total
proteins

(Ref. http://genome.annotation.jp/cyanobase/NPUN/genes)