UNIVERSITY OF SAN AGUSTIN

COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

APPENDIX E

Collection of Flowers and Leaves

Washing of Flowers and Leaves

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Comparison of Plumeria obtusa and Plumeria rubra

Plumeria obtusa flowers Plumeria rubra flowers

Plumeria obtusa leaves Plumeria rubra leaves

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Grinding and Weighing of Samples

Extraction
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Rotary Evaporation

Freeze-drying
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Agar Well Diffusion

Minimum Inhibitory Concentration

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Thin Layer Chromatography

Figure A.)Plumeria obtusa- a.1) viewed under 254nm; a.2) viewed under 365nm; a.3) tested
with Ninhydrin solution.a.4) tested with Ferric Chloride solution

Figure B.) Plumeria rubra- b.1) viewed under 254nm; b.2) viewed under 365nm; b.3) tested
with Ninhydrin solution; b.4) tested with Ferric Chloride solution

Figure C.) Combined- c.1) viewed under 254nm; c.2) viewed under 365nm; c.3) tested with
Ninhydrin solution; c.4) tested with Ferric Chloride solution.
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Agar Well Diffusion Assay Results with S.aureus

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Agar Well Diffusion Assay Results with E.coli

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Minimum Inhibitory Concentration Results

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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Statistical Analysis

For S. aureus
Report
Inhibition

Treatment Mean N Std. Deviation

P obtusa flower extract
29.8333 6 .98319

P rubra leaf extract .0000 6 .00000

Combination 28.1667 6 1.60208
Ampicilin 49.7778 9 1.92209
Total 29.4815 27 18.58529

ANOVA
Inhibition

df F Sig. Interpretation

Between Groups Statistically

3 1450.383 .000
significant
Within Groups 23
Total 26
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Multiple Comparisons
Dependent Variable: Inhibition
LSD

Mean 95% Confidence Interval

Difference (I-
(I) Treatment (J) Treatment J) Std. Error Sig. Lower Bound Upper Bound
P obtusa flower P rubra leaf extract 29.83333* .82727 .000 28.1220 31.5447
extract Combination 1.66667 .82727 .056 -.0447 3.3780

Ampicilin -19.94444* .75519 .000 -21.5067 -18.3822

P rubra leaf extract P obtusa flower
-29.83333* .82727 .000 -31.5447 -28.1220
extract
Combination -28.16667* .82727 .000 -29.8780 -26.4553
Ampicilin -49.77778* .75519 .000 -51.3400 -48.2155
Combination P obtusa flower
-1.66667 .82727 .056 -3.3780 .0447
extract
P rubra leaf extract 28.16667* .82727 .000 26.4553 29.8780
Ampicilin -21.61111* .75519 .000 -23.1733 -20.0489
Ampicilin P obtusa flower
19.94444* .75519 .000 18.3822 21.5067
extract
P rubra leaf extract 49.77778* .75519 .000 48.2155 51.3400

Combination 21.61111* .75519 .000 20.0489 23.1733

*. The mean difference is significant at the 0.05 level.

 UNIVERSITY OF SAN AGUSTIN
COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

For E.coli

Report
Inhibition2

ETreatment Mean N Std. Deviation

P obtusa 11.1667 6 .75277

P rubra 12.6667 6 1.36626
Combination 12.1667 6 1.32916
Ampicilin 9.5556 9 .88192
Total 11.1852 27 1.64169

ANOVA
Inhibition2

df F Sig. Interpretation

Between Groups Statistically

3 12.068 .000
significant
Within Groups 23
Total 26
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COLLEGE OF HEALTH AND ALLIED MEDICAL PROFESSIONS

Multiple Comparisons
Dependent Variable: Inhibition2
LSD

Mean Difference 95% Confidence Interval

(I) ETreatment (J) ETreatment (I-J) Std. Error Sig. Lower Bound Upper Bound

P obtusa P rubra -1.50000* .62811 .026 -2.7993 -.2007

Combination -1.00000 .62811 .125 -2.2993 .2993

Ampicilin 1.61111* .57339 .010 .4250 2.7972

P rubra P obtusa 1.50000* .62811 .026 .2007 2.7993
Combination .50000 .62811 .434 -.7993 1.7993
Ampicilin 3.11111* .57339 .000 1.9250 4.2972
Combination P obtusa 1.00000 .62811 .125 -.2993 2.2993
P rubra -.50000 .62811 .434 -1.7993 .7993
Ampicilin 2.61111* .57339 .000 1.4250 3.7972
Ampicilin P obtusa -1.61111* .57339 .010 -2.7972 -.4250

P rubra -3.11111* .57339 .000 -4.2972 -1.9250

Combination -2.61111* .57339 .000 -3.7972 -1.4250

*. The mean difference is significant at the 0.05 level.