GENERAL TEST PROCEDURE (GTP) Page 1 of 4

Title Estimation of Free Chlorine Effective Date 04/01/05

GTP Number GTP 117-02 Review Date 03/01/07

REVISION SUMMARY

GTP Number Date Revised Reason for Revision

GTP 056 - 00 01/07/2002 First Issue
GTP 117 - 01 05/03/2003 Renumbering of GTP and Format Changed
GTP 117 - 02 04/01/2005 The format of the GTP is changed as per SOP QA
030-00 and the procedure is revised.

1.0 PURPOSE
1.1 To lay down the procedure for determining the presence of free chlorine in water
samples.

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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 GENERAL TEST PROCEDURE (GTP) Page 2 of 4

Title Estimation of Free Chlorine Effective Date 04/01/05

GTP Number GTP 117-02 Review Date 03/01/07

1.2 Chlorine will liberate free iodine from potassium iodide (KI) solutions at pH 8 or
less. The liberated iodine is titrated with standard solutions of sodium
thiosulphate (NA2S2O3) with starch as the Indicator. Titrate at pH 3 to 4
because the reaction is not stoichiometric at neutral pH due to partial oxidation
of thiosulphate to sulfate)

2.0 APPARATUS
2.1 Burette
2.2 Pipettes
2.3 Conical flask and stopper bottles

3.0 REAGENTS
3.1 Potassium iodide crystals
3.2 Standard sodium thiosulphate 0.1 N
3.3 Starch indicator solution
3.4 Glacial Acetic Acid

4.0 PROCEDURE
4.1 PREPARATION OF REAGENTS
4.2.1 STANDARDIZATION OF 0.1 N SODIUM THIOSULPHATE
4.2.2 Dissolve 3.249 gms of anhydrous potassium biodate KH (I O3) 2 dried at
103 ° C for 1 hr.
4.2.3 Dissolve it in 100 ml and dilute it to 1000 ml to make a final
concentration of 0.1000 N solutions.
4.2.4 Store it in a glass-stoppered bottle.

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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 GENERAL TEST PROCEDURE (GTP) Page 3 of 4

Title Estimation of Free Chlorine Effective Date 04/01/05

GTP Number GTP 117-02 Review Date 03/01/07

4.2 STANDADIZATION
4.2.1 Take 80 ml of distilled water .
4.2.2 To it add with constant stirring 1.0 of conc. H2SO4 , 10. 0 ml of 0.1 000
N KH (I O3)2 and Add 1.0 gram of KI.
4.2.3 Titrate immediately with 0.1 N Na2 S2O3 until the yellow color of iodine
is liberated iodine is discharged .
4.2.4 Add 1.0 ml of the freshly prepared starch indicator and continue titrating
until the blue color disappears.

4.3 METHOD FOR THE ESTIMATION OF FREE CHLORINE.

4.3.1 Take 500 ml sample a clean and dried conical flask.
4.3.2 Add 5.0 ml of glacial acetic acid to acidify the sample its brings the
pH3.0 to 4.0,in a flask.
4.3.3 Add 1.0 gm of KI into the sample and mix with stirring rod to
dissolve.
4.3.4 Take a clean and dried burette and fill it with 0.1 N Na 2 S2O3 upto the
zero mark.
4.3.5 No air bubble should be present in the burette.
4.3.6 Add 1.0 ml of freshly prepared solution of starch indicator in to the
sample.
4.3.7 Titrate till the blue color disappears. Note the reading of the burette.

4.4 BLANK TITRATION

4.4.1. Take 500ml of distilled water in clean dried conical flask.
4.4.2. Add 5.0 ml of glacial acetic acid to acidify the sample its brings the
pH3.0 to 4.0, in a flask.

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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 GENERAL TEST PROCEDURE (GTP) Page 4 of 4

Title Estimation of Free Chlorine Effective Date 04/01/05

GTP Number GTP 117-02 Review Date 03/01/07

4.4.1 Add 1.0 gm of KI into the sample and mix with stirring rod to dissolve.
4.4.2 Take a clean and dried burette and fill it with 0.1 N Na 2 S2O3 up to the
zero mark.
4.4.3 No air bubble should be present in the burette.
4.4.4 Add 1.0 ml of starch indicator in to the sample.
4.4.5 Perform the blank titration.
4.4.6 If blue color develops , titrate with 0.01N NA2SO3 to disappear of blue
color and record the results B is Negative
4.4.7 If no blue color occurs, titrate with 0.0282N iodine solution until a blue
color appears. Back titrate with 0.01N NA2CO3 and record the
difference. B is positive
4.4.8 CALCULATION
For determining the total available residual Chlorine in water sample:
mg Cl as Cl2/L= (A-B)*N*35.45 / ml sample
Where:
35.45 is the chlorine factor.
A=mL titration for sample
B=mL titration for blank
N= normality of NA2S2O3

4.5 ACCEPTANCE CRITERIA

The sample should not contain Not More Than 5.0 ppm

5.0 REFERENCE
5.1. American Public Health Association

END OF DOCUMENT

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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