GENERAL TEST PROCEDURE (GTP) Page 1 of 4

Title Total Hardness Effective Date 01/01/05

GTP Number GTP 116-02 Review Date 31/12/06

REVISION SUMMARY

GTP Number Date Revised Reason for Revision

GTP 057-00 10/07/2002 First Issue
GTP 116-01 05/03/2003 Renumbering of GTP and Format Changed
GTP 116-02 01/01/2005 The format of the GTP is changed as per SOP QA
030-00 and the procedure is revised.

1.0 PURPOSE
1.1 To lay down the procedure for determining the total Hardness in water samples.

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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 GENERAL TEST PROCEDURE (GTP) Page 2 of 4

Title Total Hardness Effective Date 01/01/05

GTP Number GTP 116-02 Review Date 31/12/06

1.2 Ethylenediaminetetraaacetic acid and its sodiumsalts (abbreviated EDTA) form a

chelated soluble complex when added to a solution of certain metal cations .if a
small amount of dye such as Eriochrome Black T or Calmagite is added to an
aqueous solution containing calcium and magnesium ions at pH of 10.0 +_0.1,
the solution becomes wine red .if EDTA is added to an aqueous solution
containing calcium and magnesium ions These ions will be complex and the
solution turns from wine red to blue, marking the end point of titration

2.0 APPARATUS
2.1 Burette
2.2 Conical flask
2.3 Pipettes

3.0 REAGENTS
3.1 Indicators
3.2 Buffers
3.3 Ethylene Diamine Tetra Acetic Acid
3.2 PREPARATION OF BUFFERS
3.2.1 Dissolve 16.9g of ammonium chloride (NH4Cl) in 143 ml conc.
ammonium hydroxide (NH4OH) Add 1.25g magnesium salt of EDTA
and dilute to 250ml with distilled water
3.2.2 Store solution in borosilicate glass container for no longer than 1 month .
Stopper tightly to prevent the loss of ammonia or absorption of carbon
dioxide from the atmosphere. Discard the buffer when addition of 1 or 2
ml to the sample fails to produce a desired pH of 10.0 to 11.0 at the
titration end point

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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Format No.: QA030-F02-00

 GENERAL TEST PROCEDURE (GTP) Page 3 of 4

Title Total Hardness Effective Date 01/01/05

GTP Number GTP 116-02 Review Date 31/12/06

3.3 PREPARATION OF INDICATORS

3.3.1 Eriochrome Black T
3.3.1.1 Dissolve 0.5 g dye in 100 ml. of triethanolamine (also called ethylene
glycol monoethyl or add 1.0 or 2.0 (about 0.5 mg) crystals of dry
powder in to the water sample.

3.4 PREPARATIONS OF 0.01M EDTA SOLUTIONS

3.4.1 Weigh 3.723g of analytical reagent grade EDTA dissolve in
distilled water, and dilute to 1000ml.

3.5 PREPARATION STANDARD CALCIUM SOLUTION.

3.5.1 Weigh accurately 1.0g anhydrous CaCo3 powder in to a 500ml
Erlenmeyer flask. Place a funnel in the flask neck and add, a little at a
time, 1+1 HCl until all the CaCo3 has dissolved. add 200ml distilled
water and boil for a few minutes to expel CO2 cool .add few drops of
methyl red indicator .and adjust to the intermediate orange color by
adding 3N NH4OH or 1+1HCl,as require .transfer quantitative an dilute
to100 ml with distilled water ,1ml=1.00mg CaCo3.

4.0 PROCEDURE
4.1 Take 100 ml of water sample in a clean 250 ml conical flask.
4.2 Add 2.0 ml of buffer solution to obtain a pH of 10 .0 to 10 .1

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Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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 GENERAL TEST PROCEDURE (GTP) Page 4 of 4

Title Total Hardness Effective Date 01/01/05

GTP Number GTP 116-02 Review Date 31/12/06

4.3 Add 2 drops or 50 mg of dry Eriochrome Black T indicator.

4.4 Add 0.01 EDTA slowly and continue stirring.
4.5 The solution will become blue at the end point.
4.5 CALCULATION
A X B X 1000
Hardness (EDTA) as mg CaCO3 / L = ml of sample

Where A = ml titrant for Sample

B = mg CaCO3 equivalent to 1.00 ml of EDTA titrant.

5.0 REFERENCE:
5.1 American Public Health Association Page No. 2-37 to 2-39

END OF DOCUMENT

Prepared By Checked By Approved By

Name M.SRIHARI N. RAJENDRA KUMAR SANJAY SHAH K. R. PRAKASH

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Format No.: QA030-F02-00