Effect of a Selected Lactobacillus Spp.

-Based Probiotic on Salmonella enterica

Serovar Enteritidis-Infected Broiler Chicks
Author(s): Jose L. Vicente, Alberto Torres-Rodriguez, Stacy E. Higgins, Christopher Pixley, Guillermo
Tellez, Annie M. Donoghue, and Billy M. Hargis
Source: Avian Diseases Digest, 3(1):e26-e26.
Published By: American Association of Avian Pathologists
DOI: http://dx.doi.org/10.1637/1933-5334(2008)3[e26:EOASLS]2.0.CO;2
URL: http://www.bioone.org/doi/full/10.1637/1933-5334%282008%293%5Be26%3AEOASLS
%5D2.0.CO%3B2

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Summarized from Avian Diseases, Vol. 52, No. 1, 2008, Pages 143–146

EFFECT OF A SELECTED LACTOBACILLUS SPP.-BASED PROBIOTIC ON SALMONELLA ENTERICA

SEROVAR ENTERITIDIS-INFECTED BROILER CHICKS

JOSE L. VICENTE,1 ALBERTO TORRES-RODRIGUEZ,1 STACY E. HIGGINS,1 CHRISTOPHER PIXLEY,1

GUILLERMO TELLEZ,1 ANNIE M. DONOGHUE,2 AND BILLY M. HARGIS1,*
1
Poultry Health Laboratory, Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas 72701, U.S.A.
2
USDA, ARS, PPPSRU, Fayetteville, Arkansas 72701, U.S.A.

Despite advances in the treatment of infectious diseases, pathogenic
microorganisms, including Salmonella, are an important threat to
health worldwide. This study found that the administration of either
a liquid or lyophilized Lactobacillus-based probiotic (FM-B11TM) in
the drinking water may help to reduce the incidence of Salmonella
recovery in broiler chicks.
The results of this study showed that application of either liquid or
lyophilized probiotic culture for three consecutive days in the drinking
water may help to reduce Salmonella Enteritidis recovery from young
birds, although further research is needed to elucidate the mechanism of
this response.
Evidence supporting the study results were gained when the effect
of a Lactobacillus-based probiotic (FM-B11TM) on Salmonella
enterica serovar Enteritidis (SE) recovery was evaluated in liquid
*Corresponding author. Mailing address: Poultry Health Laboratory, Depart-
ment of Poultry Science, University of Arkansas, 1260 W. Maple St., POSC 0-114,
Fayetteville, AR 72701. E-mail: bhargis@uark.edu
(Exp. 1) and lyophilized (Exp. 2) forms in two separate experiments
with two trials each. For each trial, eighty broiler chicks were
randomly allocated into two treatments: control and probiotic
culture. All chicks were challenged with SE (,104 cfu) upon arrival
at our laboratory. In both experiments, probiotic culture
was administered in the drinking water for three consecutive days
at a final concentration of approximately 106 cfu/mL, beginning one
hour after SE challenge. Cecal tonsils were aseptically removed at 24
and 72 h post-challenge, followed by enrichment and plating on XLD
agar for the presence or absence of Salmonella-typical colonies.
N In Exp. 1, a significant reduction (P , 0.05) of SE positive
samples was observed in both trials at 24 and 72 h post-
challenge (Table 1).
N Additionally, in Exp. 2, the lyophilized probiotic decreased (P ,
0.05) SE recovery at both 24 and 72 h post-challenge compared
to the control group in trial 1. In trial 2, SE evaluation was
performed only at 72 h after challenge and fewer (P , 0.001)
treated samples were SE positive (Table 2).

Copyright E 2008, American Association of Avian Pathologists, Inc. 1933-5334 online

Table 1. Therapeutic effect of Lactobacillus spp.–based probiotic (FM-B11TM) in liquid form (LIQ) administered in drinking water for 3
consecutive days on Salmonella enterica serovar Enteritidis (SE) recovery in broiler chicks.

Probiotic concentration in the drinking water (cfu/ml) SE-positive samples/total (%)

Trial Treatments 24 hr 48 hr 72 hr 24 hr 72 hr
1A Control 0 0 0 14/20 (70) 13/20 (65)
LIQ 2.0 3 106 4.0 3 106 3.0 3 106 1/20 (5)* 5/20 (25)**
2B Control 0 0 0 13/20 (65) 12/20 (60)
LIQ 9.0 3 106 6.0 3 106 5.0 3 106 4/20 (20)* 4/20 (20)*
A
6.0 3 103 cfu/bird Salmonella serovar Enteritidis.
B
7.5 3 103 cfu/bird Salmonella serovar Enteritidis.
*Values in the same column are significantly different from the control value (P , 0.01).
**Values in same column are significantly different from the control value (P , 0.05).

Table 2. Therapeutic effect of a Lactobacillus spp.–based probiotic (FM-B11TM) in lyophilized form (LYO) in the drinking water for 3 consecutive
days Salmonella enterica serovar Enteritidis (SE) recovery in broiler chicks.

Probiotic concentration in drinking water (cfu/ml) SE-positive samples/total (%)

Trial Treatments Day 1 Day 2 Day 3 24 hr 72 hr
1A Control 0 0 0 16/20 (80) 16/20 (80)
LYO 7.0 3 105 5.0 3 105 2.0 3 106 10/20 (50)* 9/20 (45)*
2B Control 0 0 0 NEC 24/25 (96)
LYO 2.0 3 106 1.0 3 106 2.0 3 106 NE 11/25 (44)**
A
1.0 3 104 cfu/bird Salmonella serovar Enteritidis.
B
5.0 3 103 cfu/bird Salmonella serovar Enteritidis.
C
NE 5 not evaluated.
* Values in the same column significantly different from the control value (P , 0.001).
** Values in the same column significantly different from the control value (P , 0.05).