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AFLP
Amplified Fragment Length
Polymorphism
1 nucleotide – up to 16 folds
3 nucleotides – up to 4000 folds
Genotyping
If there are 2 new priming
sites within 400-1600bp =
amplification
result = presence or
absence of amplification
mostly due to SNP
also deletions or insertions
Advantages
replaces RFLP in
fingerprinting technique
highly polymorphic
high reproducibility
identify through absence or
presence of fragment
characters can be increased
by changing the restriction
enzyme and nucleotide at
selective primers
Disadvantages
Dominant – lose the
codominant character
Homology – ability to
differentiate different
fragment with similar size
Mutation rate – high
homoplasy
- High levels of variation
Scoring - bias
Applications
monitoring inheritance of
agronomic traits
diagnostic in genetically
inherited disease
pedigree analysis
forensic typing (parentage
analysis)
identifying hybrids
species level relationship