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There is a relationship between intake of carbohydrates If the cells need energy, pyruvate will be brought
and lipid synthesis. Predominating hormone during inside the mitochondrial matrix and be oxidatively
sembreak (eat, sleep, rest) is insulin. decarboxylated to form Acetyl CoA.
Needs activated intermediates that are bound to a Franz Knoop elucidated the different steps initially making
particular molecule, example of the molecule of up the β- oxidation or the breakdown of fatty acids. And
which activate intermediates are bound is: since the breakdown of fatty acids is in spiral pathway, he
o ACP – Acyl Carrier Protein hypothesized that synthesis of these is exactly in the
Needs a source of electrons and protons opposite way. And later on he was proven right.
o NADPH + H coming from hexose
monophosphate (alternative carbohydrate Requirements of Fatty Acid Synthesis:
metabolic pathway) pathway and can also
come from citrate shuttle system (aka Acetyl CoA
Acetyl-CoA shuttle system) o Carbohydrates in the form of glucose after
Found in the cytoplasm, enzymes that will carry the being transformed to pyruvate enters the
different steps are coming from the cytosol. mitochondrial matrix to be oxidatively
To carry on the elongation of carbons to make up decarboxylated to form Acetyl CoA
the fatty acid, specifically the palmitic acid, we o The one initially utilized to synthesize fatty
need an enzyme complex known as Fatty Acid acids
Synthase Enzyme Complex. o Acted upon by an enzyme called Acetyl
After intake a lot of carbohydrates, this will go CoA Carboxylase or ACC enzyme
through glycolysis producing the pyruvate. Catalyzes the carboxylation
reaction producing Malonyl CoA
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Catalyzes the rate limiting step for o We start of Acetyl CoA and we elongate
synthesis fatty acids that by utilizing 4 different reactions
Single, multifunctional enzyme utilized by the cell every cycle (every time
Requires a biotin group which is a cell adds 2 Carbon Moiety to Acetyl CoA):
attached to a lysine residue of a Condensation
protein molecule 1st Reduction
Contains an allosteric regulatory Dehydration
site 2nd Reduction
Citrate – most important Answer:
regulatory factor o 7 cycles or spirals needed
Catalyzes the rate-limiting step for We have an original 2 carbon
fatty acid synthesis which forms moiety from Acetyl CoA and cells
malonyl CoA try to elongate this by adding 2
Long chain fatty acyl CoAse carbons at a time (1 spiral/cycle).
Inhibits Acetyl CoA So initially 2 carbon moiety plus 2
Carboxylase carbons added per cycle (7 cycles)
o Initial building block is equal to 16 carbons which is
o 2-carbon moiety the number of carbon of palmitic
o 16th and 15th carbon of palmitic acid acid. 2 + (2 x 7) = 16
(most common fatty acid synthesized by
the cell) came from Acetyl CoA and the
rest came from malonyl CoA Question: How many NADPH + H will the cells need to be
CO2 in the form of bicarbonate able to synthesize a 16-carbon saturated fatty acid?
o Source of carbon
ATP Answer: If the cell utilizes 7 cycles, and each of this
o Source of energy to form a covalent bond cycle involves 2 reduction reactions, it needs 14
Biotin from biocytin NADPH + H because each reduction reaction
o Being a carboxylation reaction wherein the needs one NADPH + H.
the source of carbon is CO2, it is the For every cycle, we need 2 NADPH+H because we
coenzyme needed by Acetyl CoA have 2 reduction reactions
Carboxylase
Malonyl CoA Malonyl CoA
o Utilized by the cell to lengthen the carbons
from Acetyl CoA Has 3 carbons
o As a result of carboxylation reaction, it is Source of 2 carbons used to elongate Acetyl CoA
3-carbon moiety o We only need 2 carbons, and 1 carbon
o It is the source of the 2 carbons used to should be eliminated in the form of
elongate Acetyl CoA Carbon Dioxide.
o In utilizing a 3-carbon moiety, remember Is taken over by fatty acid synthase enzyme
that the cells only need 2 carbons, so the complex, thereby elongating acetyl CoA to
other one is liberated as CO2 eventually form palmitic acid.
Signal for the cell to synthesize fatty acids
o It’s a negative allosteric effector for fatty
Question: If the cells will be synthesizing a 16-carbon fatty acid oxidation
acid, how many spirals or cycles will the cells need in order Cells are endowed with a lot of
to form palmitic acid (most common fatty acid synthesized energy and therefore can
by the cell)? synthesize a lot of fatty acids
Structure of Palmitic acid
Remember:
o Starting material is a 2-carbon moiety,
Acetyl CoA
16th and 15th carbon of Palmitic
acid came from the initial Acetyl
CoA
The rest came from Malonyl CoA
o Acetyl CoA Carboxylase (ACC) is only
initially needed to synthesize Malonyl CoA
After the cells have acted upon
the initial Acetyl CoA by means of
Acetyl CoA Carboxylase, forming
Malonyl CoA. Then the fatty acid
synthase enzyme complex will
now take over the process of Fatty acid Synthase complex
elongation.
o Fatty acid synthesis is a spiral pathway. A dimer
o Arranged in a head-to-tail manner
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A large molecule 3-OHACYL dehydrase Alpha beta trans- Dehydration
Multiple domain butenoyl ACP
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coA and oxaloacetate with the help of ATP dependent structures. For them to be able to transmit their message
citrate lyase enzyme. Acetyl CoA will be acted upon by inside the cell, they must possess their receptors
Acetyl CoA carboxylase to form malonyl-CoA and thereby expressed on the membrane. Epinephrine (amine hormone)
will be taken over by fatty acid synthase to form fatty acids is derived from tyrosine. The receptor in the plasma
(such as palmitate). Oxaloacetate will be subsequently membrane, epinephrine and glucagon have to bind to the
reduced by malate dehydrogenase cytosolic with the help receptor, forming a hormone-receptor complex to activate
of NADH to form malate. Malate can either go again inside the G-protein. Activated G-protein then activates AC
the mitochondrial matrix or it can be oxidatively (Adenylyl cyclase), that tries to cyclycize ATP to form the 2nd
decarboxylated by malate enzyme (or NADP-dependent messenger, which is cAMP (Cyclic AMP). cAMP activates
malate dehydrogenase enzyme) with the help of NADP, the inactive protein kinase A. If there is an activated
which after oxidizing malate back to pyruvate, the cells protein kinase A, proteins and enzymes will receive
generate NADPH+H (much needed reducing equivalent phosphate groups. Therefore, these enzymes and proteins
needed for fatty acid synthesis). are inactivated due to their phosphorylation.
Therefore, if we will try to count the number of citrate There is a predominance of epinephrine and glucagon
molecules that goes out into the cytoplasm, creating acetyl during starvation. Insulin is exerting the opposite effect of
CoA molecules that the cells utilize for fatty acid synthesis, glucagon. Insulin is also a peptide hormone, which has a
How many of this will be generated, that will contribute to receptor on the membrane. The receptor of insulin is called
the 14 NADPH+H to complete the synthesis of palmitic receptor tyrosine kinase. So from this moment, when it
acid ? binds, after forming a hormone-receptor complex, one of
the actions is to stimulate an enzyme called
NADPH+H = 14 phosphodiestarase (3’,5’-cyclic AMP phosphodiesterase) to
transform cAMP to 5’-AMP. Therefore, protein kinase is not
Acetyl CoA needed by the cell to synthesize activated… Enzymes will be in the dephosphorylated form.
palmitic acid = 8 Activated form of Acetyl CoA carboxylase is in
dephosphorylated form, therefore can do its job to
Citrate = 8 (because from 8 citrates, 8 acetyl CoA
transform Acetyl CoA into Malonyl CoA. But with the
will be formed by citrate lyase enzyme)
predominance of glucagon and epinephrine, Acetyl CoA is
OAA= 8 converted to pyruvate (8 NADPH+H is inhibited.
generated)
Remember: Enzymes catalyze a specific reactions and
***14 NADPH+H – 8 NADPH+H = remaining 6 NADPH+H therefore specific names:
Protein kinase A activation is dependent on cAMP.
(which can be provided by HMP or Hexose Monophosphate Protein kinase G activation is dependent cGMP (a second messenger
Pathway) that activates protein kinase G)
Protein kinase C activation is dependent on calcium ions (C2+)
As long as we have citrate in the cytoplasm, Acetyl CoA
Carboxylase is stimulated. Covalent modification:
Inactive form Acetyl CoA carboxylase is in the form of Attachment of phosphate group to enzymes and
monomers. If there are 40 monomers, they try to aggregate proteins
to form the active form (protein-protein interaction). One way of which the cells try to stimulate/ inhibit
2 opposing pathways
o Ex: Glycogenolysis & Glycogenesis
(Carbohydrate metabolism)
By attaching a phosphate through glucagon
Glucagon and epinephrine 1st messengers (they
can transmit messages inside the cells through
cAMP dependent protein kinase A cascade).
Through covalent modifications whereby activity of
most enzymes are activated, while some are
inhibited.
o So.. Depende sa enzymes.. Merong ibang
enzymes na pag naka-tanggap ng
phosphate group, ma-aactivate, meron
naman iba na ma-iinhibit.
Sa ACC, gumagana lang siya
kapag dephosphorylated sila. So
dapat walang phosphate group.
o Our cells do not have the luxury to
synthesize molecules when there’s a need
for energy. When there is a need for
There is insulin predominance during FA synthesis. The energy, cells mostly induce catabolism to
confirmation of Acetyl CoA carboxylase is in the release a lot of electrons and protons to
dephosphorylated form (activated form). Insulin and be harnessed in ETC. After being carried
by coenzymes such as FAD and NADH+H.
glucagon are peptide hormones, so they cannot just
If we transfer electrons and protons
traverse the plasma membrane due to the nature of the through NADH+H, it will create a PO ratio
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of 3:1 (3 moles of ATP per 1 mole of NADH and series 3 leukotrienes). Furthermore, through a
+ H) and ½ of oxygen molecule (O2) or 1 desaturase delta 5, there will be an introduction of double
oxygen atom will be used as electron bonds in between carbons 5 and 6 to form arachidonic acid
acceptor to form metabolic H2O. If FADH2
(precursor of series 2 prostaglandins and series 4
(2:1), 2 moles of ATP as against 1 atom of
oxygen that serves as the final electron leukotrienes). This can also be elongated after elongation
acceptor to form metabolic water. desaturation to form another precursor for eicosanoid
The opposite is brought about by the synthesis called tinodonic acid or eicosapentaenoic acid
predominance of insulin removal of phosphate (precursor of series 3 prostaglandins or series 5
group leukotrienes)
o Wherein it stimulates phosphodiesterase
not adenylyl cyclase
o Insulin also stimulates phosphatase
Phosphatase removes phosphate dihomo-gamma-linolenic acid
group.
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TRANSPORT OF FATTY ACIDS IN THE BLOODSTREAM:
1. PLASMA ALBUMIN
2. LIPOPROTEINS
In β- oxidation, Malonyl CoA inhibits carnitine acyl
transferase 1 (enzyme needed for beta oxidation) a. Carriers of esterified fatty acids, primarily
as triaglycerols
Insulin
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2 kinds of unsaturated fatty acids:
If that is the case, the cleavage will be at the site wherein it releases
propionyl CoA (a 3-carbon moiety). Propionyl CoA is transformed to
Succinyl CoA and eventually enters Kreb’s cycle. Beta carbon can be
subjected to a normal beta oxidation, releasing Acetyl CoA (2-carbon
moiety). After that, it releases propionyl CoA again.
Initially, there is a double bond situated in carbon 9 and
10. Initially, oleoyl CoA is subjected to 3 cycles of normal
B‐oxidation. After removing 6 carbons, the initial carbon Refsun’s Disease refers to the accumulation of phytanic
7 becomes carbon 1, carbon 8 now becomes our alpha acid especially in the brain, secondary to enzyme
carbon, and carbon 9 becomes the beta carbon. The beta
deficiencies.
carbon as we can see is now involved in a double bond,
which our cells will not be able to utilize the carbon with
double bond. So the cells isomerizes cis‐delta‐3‐ OMEGA OXIDATION
Dodecenoyl CoA forming trans‐delta‐2‐Dodecenoyl CoA,
which can be subsequently hydrated with a mole of Last carbon or the omega carbon is transformed
water to form L‐beta‐Hydroxy‐Decanoyl. After this we into a carboxylic carbon.
can now utilize the normal B‐oxidation cycle to degrade
this kind of fatty acid.
o If this happens, both sides can be
subjected to normal B-oxidation
2. Polyunsaturated Fatty Acids
PEROXISOMAL OXIDATION
Flavin-dependent reaction
Amount of energy in the form of ATP is less
because electrons are not transformed to ETC, but
instead transferred to a molecular oxygen, forming
hydrogen peroxide
Hydrogen peroxide is a harmful compound that is
degraded to oxygen and water by the catalase
enzyme.
ALPHA OXIDATION
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o So instead of FA synthesis, the Acetone + dehydrogenase
predominant pathway is beta-oxidation,
forming a lot of acetyl Coa. Acetyl CoA Liver is the site of ketogenesis. But with respect to energy
goes to kreb’s cycle. utilization, the liver cannot utlize them as source of energy
due to the absence of succinyl CoA -aceto-acetate CoA
transferase (thiophorase). “Ako ang nag saing, iba ang kumain.
Awww Emo much:(”
EICOSANOIDS
PROSTAGLANDIN
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Series 3 prostaglandins – there are 3 double bonds outside o Cyclooxygenase activity is inhibited by
the cyclicized region from eicosapentaenoic acid or aspirin (acetylsalicylic acid) and ibuprofen
timnodonic acid (Non-steroidal anti-inflammatory drugs)
2 isoforms of cyclooxygenase
Among the unsaturated fatty acids, there are 2 essential COX1 – a constitutive
polyunsaturated acids: enzyme (it’s always
present whether or not
1. linoleic acid (true essential) there are substrates)
a. From this, we can synthesize dihomo- o When blocked,
gamm-linolenic through a GI bleeding and
dehydrogenation reaction gastritis
2. linolenic acid o Needed for the
maintenance of
integrity of the
membranes of
intestinal cells
COX2 – an inducible
enzyme
o Made only in
response
through
inflammatory
initiators such as
cytokines
o The one that is
blocked
o Celecoxib are
LEUKOTRIENES specific
inhibitors
o Catalysis through lipooxygenase o Cyclooxygenase activity is inhibited by
o Series 3 from eicosatrienoate aspirin and ibuprofen (Nonsteroidal anti-
o Series 4 from arachidonic acid inflammatory drugs)
o Series 5 from eicosapentaenoate
LIPOXINS
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