Professional Documents
Culture Documents
13
27.2.08
University of Mumbai
Ordinances, Regulations
and
Syllabus
In
Bioinformatics
at
M.Sc. Degree course
1
Introduction
Programme Overview
2
Objectives of the course
3
Eligibility
4
Programme Structure
Work load: · Four periods per week per paper where each
period is of ONE hour duration.
· Four practical per week. Each practical is of
Four periods where each period is of ONE
hour duration.
· One Seminar per Week. Each seminar is of
ONE hour Duration for a batch of TEN students.
· Guidance to the students for projects.
· Instrument technician - 01
· Technical assistance - 02
· Lecturer - 03 ( Full Time )
· Lecturer - 01 ( Part Time)
· Remaining workload will be shared with visiting faculty.
5
Faculty
The visiting Faculty will be from a post equivalent to that of Senior Lecturer
level with PhD and not less than 5 years of research experience or with
experience in industry not below Assistant Manager Level.
Mark-list
The mark-list of the students must indicate titles of papers in the syllabus.
6
SYLLABUS IN BRIEF
M.Sc Part I
M.sc Part II
7
SYLLABUS FOR M.Sc BIOINFORMATICS
MODULAR DISTRIBUTION OF TOPICS
M.Sc PART I
1.4 Integration of Metabolic Pathways and 1.8 Recombinant DNA technology (15)
Bioenergetics(15)
8
PAPER III Biostatistics
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SYLLABUS FOR M.Sc BIOINFORMATICS
MODULAR DISTRIBUTION OF TOPICS
M.SC PART II
6.3 Pair wise sequence alignment(15) 4.7 Taxonomy and Phylogeny (15)
10
PAPER VII Application of Bioinformatics
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PAPER I
FUNDAMENTALS OF BIOLOGY
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1.5 Enzyme Kinetics(15)
· Introduction to Enzymes.
· Units of activity, coenzymes and metal cofactors.
· Temperature and PH effects,
· Michaelis – Menten Kinetics,
· Feed back inhibition and activation.
· Ribozyme and abzymes
13
LIST OF REFERENCE BOOK
14
PAPER II
15
2.6 Genomics (15)
16
LIST OF REFERENCE BOOK
17
PAPER III
BIOSTATISTICS
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3.5Stochastic Process (15)
· Poisson Process
· Markov chains
· Transition Probabilities
· M.C with Absorbing state and no absorbing state
· Stationary distributions
· Modeling
·One-way classification
·Two – way classification model with and without interaction effects, one
observation per cell and r observations per cell
·Mutiway classification model
·Regression analysis
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LIST OF REFERENCE BOOK
20
PAPER IV
21
4.4 SQL (15)
· Select statement
· Data definition statement; Data Manipulation Statements;
· Data Control Statement.
· Other Database Objects (Views, Sequences, Synonyms); Introduction to
Application.
· Development using Visual Basic; Working with code and forms; Variables,
Procedures and controlling
· Prorgram Executor; Standard Controls; Data Access Using data Control;
Connecting to Oracle / SQL Database using Visual Basic.
22
LIST OF REFERENCE BOOK
23
PAPER V
24
5.5 Drug Delivery systems (15)
· Types of drug delivery system.
· Dendrimers
· Molecularly imprinted polymers(MIPs), MIPs in Drug delivery,
· Introduction to Biomedical micro- and nano-technology
· Liposomes: Enzyme-activated, Phototriggering, Thermosensitive
· Orphan GPCRs
· Identification of Orphan GPCRs
· Reverse Pharmacology
· Reverse Pharmacology for Orphan GPCRs
· Deorphanisation
25
LIST OF REFERENCE BOOK
26
PAPER VI
· Use of Pairwise Alignments for analysis of Nucleic acid and Protein Sequences
and interpretation of results
27
6.5 Heuristic methods of sequence alignment (15)
· BLAST algorithms
· PSI BLASTalgorithm
· FASTA algorithms
· Various versions of basic BLAST and FASTA. Online use of the tools and
interpretation of the results
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LIST OF REFERENCE BOOK
1. Westhead, D.R., Parish, J.H. & Twyman, R.M., Instant Notes: Bioinformatics, 2002,
BIOS
2. Higgs, P.G & Attwood. T.K., Bioinformatics and Molecular Evolution, 2005, Blackwell
Publishing.
3. Campbell, A.M & Heyer, L.J., Discovering genomics, proteomics and bioinformatics,
2003, Benjamin Cummings.
4. Mount, D.W., Bioformatics sequence and genome analysis, 2nd Ed., 2004 Cold
Spring Harbour Laboratory Press.
5. M. Lesk. (2002) Introduction to Bioinformatics Oxford University Press
6. G.B Fogel D.W Corne (2002) Evolutionary Computation in Bioinformatics
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PAPER VII
APPLICATION OF BIOINFORMATICS
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7.5 Machine Learning Techniques (15)
31
LIST OF REFERENCE BOOK
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PAPER VIII
CONCEPTS IN COMPUTING
· Introduction to JDBC
· Client Server Application
· Java Drivers
· java.sql Package
· Executing SQL Statements
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8.5 Introduction to Perl and data types (15)
· Introduction: What is Perl? Why use Perl in Bioinformatics? History of Perl,
Availability, Support, Basic Concepts
· Scalar Data: What Is Scalar Data?, Numbers, Strings, Scalar Operators, Scalar
Variables, Scalar Operators and Functions
· Arrays and List Data: What Is a List or Array? Literal Representation,
Variables, Array Operators and Functions, Scalar and List Context
· Control Structures: Statement Blocks, basic I/O.
· Hashes, Hash Variables, Literal Representation of a Hash, using Hashes.
· Subroutines, System and User Functions, The local Operator
· Using Bioperl.
· Filehandles and File Tests, Opening and Closing a Filehandle, die, Using
Filehandles,
· Directory Access, Moving Around the Directory Tree, Globbing, Directory
Handles, Opening and Closing a Directory Handle, Reading a Directory Handle
· Process Management,
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LIST OF REFERENCE BOOK
35
PRACTICALS SYLLABUS FOR M. Sc. BIOINFORMATICS
Part I Practicals
PRACTICAL I TO IV
PRACTICAL I
· Identification of various stages in mitosis and meiosis from permanent slides.
· 2D separation of amino acids, plant pigments using paper chromatography
· Biochemical estimation of carbohydrates:-
· Biochemical estimation of proteins:-
· Biochemical estimation of lipids:-
· Biochemical estimation of DNA & RNA
PRACTICAL II
PRACTICAL III
Report on Industrial visits – minimum FOUR.
PRACTICAL IV
· Introduction and overview of general computer operation ( Windows and Linux
platforms). Use of various search engines, email etc.
· Writing programs based on C and C++ viz ;
· Finding prime numbers, Fourier series
· Reading a DNA sequence, Translating into RNA sequence.
· From DNA or RNA to Polypeptide sequence..
· Use of SQL Server and mySQL,
· Writing Basic SQL select queries.
· Creating databases in SQL and Oracle, using various data types and granting
privileges.
· Use of statistical packages such as SPSS, metlab etc.
· Population distribution studies,
· Performing statistical tests such as chi square, ANOVA etc.
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PRACTICALS SYLLABUS FOR M. Sc. BIOINFORMATICS
Part II Practical
PRACTICAL V TO VIII
PRACTICAL V
· Different types of search engines & important sites viz NCBI, EBI, Swiss-Prot,
PDB etc.
· Biological Databases: Study of different Biological databases (esp. the ones
given below), Format, their distinguishing features, Uses and Applications.
· Sequence databases: EMBL, DDBJ, GenBank, Uniprot, PIR, TrEMBL
· Domain database: Prosite, PRINT, Pfam, BLOCK
· Structure database:PDB, use of Rasmol, protein explorer.
· Specialised database: KEGG, PUBMED, OMIM
· Use of BLAST and its variants (especially Psi-BLAST) for the identification of
very similar and divergent sequences.
· Use of FASTA and its comparison with BLAST.
· Multiple sequence alignment using ClustalW and preparation of simple
phylogenetic trees using Tree View etc.
PRACTICAL VI
· Detection of general signals, regulatory regions and promoter prediction.
· Secondary structure prediction using various available tools,
· Tertiary structure and function prediction using homology modelling and ab
initio method.
· Validation of predicted structure.
· Visualization of 3D protein structure using Rasmol, VMD etc.
· Microarry and clinical data analysis
· Use of ANN or SVM as a prediction server.
· Target Structure Based Drug Design
· Active site identification,
· Characterization of target site,
· Docking
· Analysis
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PRACTICAL VII
· Preparing a sample WebPages for self in HTML .(self website)
· Writing simple programs in Java like finding prime numbers, Fourier series etc.
· Java applets and servelets.
· JDBC: connecting to a database (mySQL)
· Using perl for writing small scripts like
· Reading a DNA sequence, Translating into RNA sequence.
· From DNA or RNA to Polypeptide sequence etc.
· Using Hashes
· Creating dynamic web pages using CGI perl.
· Retrieving and writing to databases (mySQL) using perl database connectivity.
· Installing and using Bioperl
PRACTICAL VIII
· Industry Oriented Practical Training / Industrially relevant Project work. A
report should be submitted at the time of examination.
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Evaluation:
I 75 3 Hr 19
II 75 3 Hr 19
III 75 3 Hr 19
IV 75 3 Hr 19
V 75 3 Hr 19
VI 75 3 Hr 19
VII 75 3 Hr 19
VIII 75 3 Hr 19
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Practical Examination Question Paper Pattern:
I 50 13
II 50 13
III 50 13 Presentation,Submission
and Viva on Industrial
Visits
IV 50 13
Aggregate 200 80
V 50 13
VI 50 13
VII 50 13 Presentation,Submission
and Viva on Industrial
oriented work.
VIII 50 13
Aggregate 200 80
40
Minimum Infrastructure required for running the course
b. Air-conditioned Room
c. Library Facilities
e. Internet Facilities
g. Instrumental Support
41
Recommended equipment and accessories
Sr. No Equipment
1. Agarose and PAG Electrophoresis systems
2. Analytical Balance
3. Autoclave
4. Capillary Electrophoresis (with PDA & UV detectors)
5. Computers
6. Deep Freezer
7. Dissolution Test Apparatus
8. DNA Sequencer
9. Flame Photometer
10. Fourier Transform Infrared Spectrometer
11. Gas Chromatograph
12. Gel Documentation
13. HPLC with various detectors (UVNIS, E.C.D, PDA) & software
14. HPTLC Densitometer with CATS 3.0software
15. HPTLC Spotter
16. LC/MS/MS
17. Low Volume Evaporator
18. Melting Point Apparatus
19. pH - meter
20. Refrigerators
21. Solid Phase Extractor
22. Top pan balance
23. Ultrasonic bath with Temperature control
24. UV-Vis Scanning Spectrophotometer
25. Water Distillation Apparatus
26. Water Purification System
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