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Cat. No. 11668-027 Cat. No. 11668-019 Cat. No. 11668-500 Size: 0.75 ml Size: 1.5 ml Size: 15 ml Store at +4C (do not freeze)
Description
Lipofectamine 2000 is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats (e.g. 96-well). Refer to the Cell Lines database at www.invitrogen.com for a list of cell types successfully transfected. Nucleic acid-Lipofectamine 2000 complexes can be added directly to cells in culture medium, in the presence or absence of serum. It is not necessary to remove complexes or change/add medium after transfection, but complexes may be removed after 4-6 hours. Use the procedure on page 2 to transfect cells with short interfering RNA (siRNA) or Stealth RNAi. Use the procedure on page 3 to transfect cells with plasmid DNA. We recommend Opti-MEM I Reduced Serum Medium (Cat. No. 31985-062) to dilute Lipofectamine 2000 and nucleic acids before complexing. Do not add antibiotics to media during transfection as this causes cell death. Maintain the same seeding conditions between experiments. Test serum-free media for compatibility with Lipofectamine 2000 since some serum-free formulations (e.g. CD293, SFM II, VP-SFM) may inhibit cationic lipid-mediated transfection.
Note: For more tips for your RNAi experiment, refer to Seven Steps to RNAi Success. This manual is available from www.invitrogen.com/rnai or Technical Service, as are cell-type specific RNAi transfection protocols (see RNAi protocols.)
Quality Control
Lipofectamine 2000 is tested for absence of microbial contamination with blood agar plates, Sabaraud dextrose agar plates, and fluid thioglycolate medium, and functionally by transfection of CHO-K1 cells with a reporter plasmid.
Part No.: 11668.2k.pps Rev. Date: 11 July 2006
For research use only. Not intended for any animal or human therapeutic or diagnostic use. For technical support, contact tech_service@invitrogen.com.
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Surface areas may vary depending on the manufacturer. 2 Volumes of dilution medium in Step 2a & 2b of DNA or RNAi transfection protocols.
Purchaser Notification
This product is covered by one or more Limited Use Label Licenses (see the Invitrogen catalog or our web-site, www.invitrogen.com). By the use of this product you accept the terms and conditions of all applicable Limited Use Label Licenses.
Limited Use Label License No. 27: Lipofectamine 2000 Reagent Limited Use Label License No. 173: Inhibition of Gene Expression by Double-Stranded RNA Limited Use Label License No. 196: Stealth RNAi 2000-2006 Invitrogen Corporation. All rights reserved.