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AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

Problem Would there any uptake of oxygen by the crickets? Hypothesis The level of coloured liquid with crickets is increases, while the level of liquid coloured without crickets tends to decrease. Uptake of oxygen causes decrease in pressure. The coloured liquid flows towards the direction which has a lower pressure. There would be an uptake of oxygen by the crickets, measured by using the respirometer. Apparatus Micropipette Gauze cage Screw clip 2 boiling tubes Manometer tube with scales 1 cm3 syringe Two 3-way taps Stoppers with tube 25 cm3 measuring cylinder

AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

Materials Coloured solution Potassium hydroxide (KOH) solution Crickets

Procedure 1. Two boiling tubes were filled with 15 ml of KOH solution measured using a 25 cm3 measuring cylinder 2. A gauze cage was placed inside of the boiling tube and 4 crickets that were captured beforehand were placed carefully inside the boiling tube. 3. The boiling tube was then quickly closed with a stopper with inlet tubes to prevent the crickets from escaping. The other boiling tube was also closed with a stopper with inlet tubes 4. A 3-way tap was fixed to each boiling tube and made sure that the shorter hand of the tap was pointing up for the boiling tube without the crickets (closing the top outlet to the outside) and the other boiling tube with the crickets was made sure that all the 3 outlets were open. 5. A manometer tube was filled with approximately 100 l of coloured solution using a micropipette. 6. The capillary manometer is then calibrated to get an equal reading on both sides using 1 cm 3 syringes. The reading is recorded as initial reading. 7. Before fixing it to the repirometer, the level of the coloured solution in the manometer tube was determined and recorded. 8. The apparatus was then set up as shown in the figure below.

AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

9. The stopwatch is started and the set up is observed after 30 minutes 10. After 30 minutes, the level of the coloured solution was then noted for any changes and the data collected was recorded into a table. 11. The rate of oxygen uptake is calculated by using formula: Rate of oxygen uptake = distance of coloured liquid moved (cm3) Time taken (min)

Result

AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

Level of coloured solution on manometer / cm

Initial reading Final reading (after an 30 minutes) Distance coloured liquid moved = = = Given the time taken is 30 minutes,

5.8

5.9

Final Reading- Initial Reading (5.9-5.8) 0.1cm

Rate of oxygen uptake = distance of coloured liquid moved (cm3) Time taken (min) = 0.1 cm 30 min = 0.00333 cm min-1 (3 significant figures)

Therefore, the rate uptake of oxygen in respiration for cricket is 0.00333 cm min-1 Discussion Method of experiment After the crickets place in the gauze, The boiling tube was then quickly closed with a stopper with inlet tubes to prevent the crickets from escaping. The other boiling tube was also closed with a stopper with inlet tubes. Besides that, the coloured liquid in the manometer is measured at equal reading as constant which is 5.8 cm. It was ensured that no air bubbles were trapped inside the manometer tube. Before fixing it to the repirometer, the level of the coloured solution in the manometer tube was determined and recorded.

AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

After the apparatus has been set up, the three-way taps are opened so as to allow air passage from surrounding and the test tubes into the capillary. The manometer was also ensured stand vertically and properly in order to get valid level of reading. Before the boiling tube containing the crickets was connected to manometer, the 1cm3 is used and pressed to get the same level of reading of coloured liquid for both arm of manometer. This will correct the estimate of oxygen uptake. Analyses By referring to the result obtained from the experiment, the rate of oxygen uptake are showing a positive result by observing an increase in the level of coloured liquid in the capillary U-tube manometer. An increase in the level of coloured liquid indicates that the air between the coloured liquid and the oxygen has been consumed. Initially, the level of coloured liquid is 5.8 cm3 and it gradually increased to 5.9 cm3 after 30 minutes. After 30 minutes there is no change in the level of the coloured liquid but the result do not include any readings after the period of 15 minutes. After use the formula for rate of oxygen uptake, it is shown that the crickets in this experiment had 0.00333cm min-1. In this experiment, the investigation was carried out to determine the oxygen uptake by the crickets. The problem was raised due to the fact that under normal conditions, most animals use up oxygen to carry out aerobic respiration. To do this, a respirometer was used to measure the uptake. The underlying concept behind the respirometer is the level of the coloure solution would change in correspond to the pressure difference between the gases present in each boiling tube. At the start of the experiment, the pressure of the gases present in both boiling tubes would be the same and both would contain the same proportion of oxygen, carbon dioxide and other atmospheric gases. Since in both boiling tubes there was KOH solution present, the carbon dioxide was absorbed by the solution, so that no carbon dioxide would be present throughout the experiment. As the experiment progressed and an30 minutes had passed, the pressure in the boiling tube with the insect show a decrease, indicated by the increase in the level of the coloured solution connected to the boiling tube with the cricket by 1 cm. One reason behind this was simply due to the oxygen uptake by the crickets for aerobic respiration. Since aerobic respiration
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AZMIL PUTRA BIN SYAFARUDDIN 10M9 2009609648

requires oxygen to produce ATP, the oxygen in the boiling tube was used up by the crickets. This would lead to the decrease in the partial pressure of the oxygen in the boiling tube. Decrease in the amount of carbon dioxide also led to the pressure drop. Although the crickets also removed carbon dioxide gas due to respiration, this did not increase the pressure as it was quickly removed by the KOH solution.

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