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Monitoring
Comission Decission 97/747/EC Directive 96/23/EC
Sample should allow tracking Taken at farm level or at packing centre 12 eggs or more 1 sample per 1000 Tonnes of annual production. Minimum 200 samples. 30 % taken at packing centres
Monitoring
Comission Decission 97/747/EC Directive 96/23/EC
Groups tested: 70 % tested for groups A6, B1 and B2(b) A6 :pharmacologically active substances for which no maximum levels can be fixed (banned) B1 :antibacterial substances, including sulfonamides and quinolones B2 (b ) Anticoccidials, including nitroimidazoles 30 % according to situation, but must include B3 (a) Organochlorine compounds, including PCBs
Limits
Drugs: MRLs in Unofficial consolidated version of Annexes I to IV of Council Regulation 2377/90. Updated 22/12/2004. Limits for pesticides. Directive 2002/32/CE. Limits for Dioxins. Directive 2001/102/CE.
Mycotoxins
other pesticides polychlorinated biphenyls brominated flame retardans mineral oil hydrocarbons
Heavy metals
In general levels are greater in albumin
Lead
Chronic and cumulative Accidental contamination:
airborne deposition in plants. Drinking water. Soft waters and leaded pipes. Paints,
Affects enzymes, anemia, renal toxicity, carcinogen, cardiovascular and neurobiological impact Levels are low in eggs (0.003 to 0.259 mg/kg in European surveys) PTWI= 0.020-0.025 mg/kg body weight
Mercury
Chronic and cumulative Teratogenic and carcinogenic, damages CNS Organic is more toxic. Feeding seed grains treated with methylmercuric chloride to prevent insect infestation. 93 mg/kg in albumen and 5.8 mg/kg in yolk. Accidental contamination in Washington State in 1979 by mercurial fungicides. Nowadays are banned Maximum EU 0.3 mg Hg/kg in calcium carbonate European survey. Avg=0.0013- 0.005 mg/kg. Max= 0.01 mg/kg PTWI= 0.005 mg/kg body weight/week.
< 0.0033 mg/kg body weight/weeek as methyl mercury
Cadmium
Toxicity: long term exposure Teratogenic and carcinogenic, kidney, bones, neurotoxic Sources: plants grown in Cd rich soils, impurities in minerals, drinking water Present in egg yolk. Transfer is low, retained in follicle walls. Low levels in eggs in surveys (0.001-0.01 mg/kg). PTWI = 0.42 mg/person/week
Arsenic
Skin lesions and neurological effects Water, arsenicals (discontinued), pesticides As3 more toxic than As5 In the egg, yolk/albumen=1/3 At increasing levels in feed a plateau is reached rapidly Low levels in eggs in surveys (0.001-0.006 g/g). PTWI= 0.015 mg/kg body weight/week
Fluorine
In phosphate supplements (should be defluorinated). Genotoxic, skeleton (bone fracture), dental fluorosis and hypomineralization of enamel. Transferred to eggshell. High F diets result in 0.4-1.0 mg F/kg fresh albumen, and 3.1-8.4 mg F/kg dry fat free yolk. Risk of bone effects > 5 mg F/day
Nitrates
Present in water and vegetables Anaemia Nitrites in water. Formation of methaemoglobin Fast excretion in urine. No bioaccumulation. Little information in eggs. Content probably low? Low compared to other sources (water)
2
5 350 0.1 0.5
Radionuclides
Transfer is higher than for other animal products Experience from Chernobyl accident: 137Cs and 131I from contaminated cereals. Countermeasures: Iron (III) hexacyanoferrates U and Po in phosphates in Israel. Dose equivalent for all poultry products (70% of meat) is 0.04 mSv/year. (According to ICRP individual doses of less than 10 Sv/year are negligible) Limits according to Council Regulation (EURATOM) No 2218/89 (Bq/kg)
125; 131I: 500; 239Pu and 241Am: 20; 134Cs and 137Cs and all t1/2 > 10 days: 1000 3H, 14C and 40K are excluded
90Sr:
Mycotoxins
Eggs < animal feeds. Unlikely acute toxicity Aflatoxin B1, M, ochratoxin A are carcinogenic and must be monitored. Fumonisin, Vomitoxin, and zearalenone found at very low levels or not found ata all when contaminated feeds are given to hens.
Ochratoxin A
No information in eggs of laying hens In Japanese quail there is transfer to eggs. Contribution of products of animal origin is not more than 3 % of the burden
Aflatoxin B1
Residues when feed is 500 g B1/kg feed but not at 300 g B1/kg feed Transfer 4615:1 in yolk, 3846:1 in albumen In Japanese quail transfer is higher After 7 days withdrawal only traces in eggs Limit in feeds is 0.02 mg B1/kg feed Limit in eggs is 3 pg WHO-PCDD/F-TEQ/ g fat Maximum level based on ALARA
T2 Toxin
Inmunosupresive and carcinogenic 1 mg T2 for 8 days 0.9 gT2/egg Limits in Israel and Russia: 0.1 mg T2/kg feed
Organochlorine pesticides
Transferred to eggs in many cases Very persistent. DDT (metabolites) still can be found in eggs more than 25 years after use was discontinued. Banned for most applications in Europe and USA but still used in some third world countries: Monitoring of eggs and feed ingredients Limits for complete feeds and supplemental fats: see manuscript, adapted from EU 1999
Prevention
At farm level:
Good production practices.
Control and traceability of feeds Wtihdrawal time for drugs Control of drinking water
Feed
Monitoring of ingredients. European legislation
HACCP Traceability