Professional Documents
Culture Documents
Mark Hargrove
Dept. of Biochemistry, Biophysics, & Molecular Biology
Mainpointsfromthislecture.
Whystudybiochemistry?
Howwillthiscourserun?
Theimportanceofbiochemicalunity
Theimportanceofwaterandweakforces
AreminderofpHandbasicthermodynamics
Overview of Biochemistry
courses
the context and level of detail will usually be different in this one
Mainpointsfromthislecture.
Howwillthiscourserun?
(letslookatthesyllabus)
Historical
Development of
Biochemistry as a
Discipline
Physical and chemical
sciences characterize
biomolecules in chemical
terms
Biochemistry is a
young field!
Theearthisold.
ClairPatterson
19221995
HewasfromMitchellvilleIowa,andmeasuredtheageoftheearth:
4.55billionyears.HisworkalsoleadtotheCleanAirAct(1970)
Theroadmapoflife
prebioticworld
Biochemicalunityunderliesbiologicaldiversity
Biochemicalunityunderliesbiologicaldiversity:
Thisisoneobservationthatsupportsspeciationandacommon
biochemicalunderstandingofbiology
Generalbiochemistryissharedbytheseorganisms:
ItdoesntHAVEtobethisway,yetitis.
Molecular Scale in
Biological Systems
Atoms - Angstrom units, 0.1
nm
Prokaryotic cells - 1 to 10 m
Eukaryotic cells - 10 - 100 m
Eukaryotic organisms - 106 m
(1 m), so very many cells in
the organism
% WATER
83
79
79
77
94
87
36
12
water
nutrients used for generation of energy
building block compounds used for assembly of polymeric
biomolecules
Compartmentalization through the hydrophobic effect
Hydrophobic organic molecules are not soluble in water
hydrophobic molecules, mostly lipids, separate from the waterbased solution
barriers are created between water-based compartments
Buffer for temperature and pH
high heat capacity means temperature stays relatively constant
chemical system for absorption and release of protons allows the
concentration of protons (pH) to remain relatively constant
and Participation in Chemical reactions...
Structure of Water
tetrahedral arrangement of sp3
orbitals around oxygen
Hydrogenbondingisveryimportantinbiochemistry
~10kJ/mol
S-H------O
xamples of H-bonding
Individual water molecules can form up to four H bonds, one for each
H nucleus and 2 for the O nucleus
transient: each molecule breaks and reforms bonds every ~10-12 sec
Waterisimportantalsobecauseweakforcesruleinbiochemistry
Electrostatic
1to5kJ/mol
vanderWaals
~2kJ/mol(peratom)
Thehydrophobiceffect
Ch.12
(Waxes)
Examples of
water molecules
separating from
nonpolar atoms
Ch. 12
bonds is necessary
individual weak bonds cannot mediate
a stable association
many weak bonds summed together
are responsible for the association
Molecular recognition is often reversible
Thingsweneedtoknowaboutwaterchemistry
+
H + OH
H 2O
[H ][OH ]
K eq =
[H 2O]
14
K w = [H ][OH ] = 1x10 M
2
[H ] = [OH ] = 1x10 M
AcidsandBases
+
Acid
H + Base
RCOOH H + RCOO
NH 4
H + NH 3
[H + ][A ]
Ka =
[HA]
pH Scale
Remember that the scale is logarithmic: a
Household bleach
DefinitionofpHandpKa
[H + ][A ]
Ka =
[HA]
+
pH = log(1/[H]) = log([H ])
(WaterpH=7)
pK a = log(1/K a ) = log(K a )
[H + ][A ]
log(K a ) = log(
)
[HA]
andsolveforpH
[A ]
pH = pK a + log(
)
[HA]
HendersonHasselbalchequation
The HendersonHasselbalch
Equation
This equation is an algebraic rearrangement of terms from the equation
that defines Ka
pH = pKa + log ([A-]/[HA])
Memorize this equation
Using the HH equation we can set the pH of a solution by knowing the pKa
and varying the amount of A- and HA
A- is provided by adding the salt, (such as Na +A-), which completely
dissociates
HA is added directly
the degree of dissociation is very small, so it can be neglected in the
equation
Relevance of pKa
in biochemistry.
The functional groups with a low pKa (<7) are
deprotonated at neutral pH (like COOH is in the form
COO-), while groups with high pKa (>7) are protonated at
neutral pH (like NH3 is in the form NH4+).
[A ]
pH = pK a + log(
)
[HA]
Buffers
AtpH=pKa,[A]=[HA]
CH3COOH
1NHCl
1NHCl
Thermodynamicsandbiochemistry
1.Thetotalamountofenergyisconstant
2.Entropy(S)increasesforspontaneousreactions
3.S=0at0Kinaperfectlyorderedcrystal
Howcanlifeexistifentropymustalwaysincrease?
Entropyincreasesinthesurroundings
ThisistheimportanceofGibbsFreeEnergy;ittakesintoaccounttheentropyofthesystemanditssurroundings
G=HTS
Letstakeacloserlookattheseterms.
Enthalpy
The enthalpy change, H, is the heat released or absorbed for a
Polymeric Biomolecules
Nutrition
Medicine
Biomaterials
Nano-machines
Genetic Engineering
Feeding the entire world
Unlimited clean-burning fuel
Glowing mice and photographic bacteria