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Power
Equipment & Reagents
Electrophoresis equipments
Power supply
Casting tray
Gel combs
Gel casting tray & combs
Preparing the Casting Tray
Seal the edges of the casting tray and put in the combs.
Place the casting tray on a level surface.
None of the gel combs should be touching the surface of
the casting tray.
UV light transilluminator
Procedure
(1)Prepare 2% agarose gel:-Combine2g agarose with100 ml
TBE buffer(the TBE concentration should be (1x)otherwise
dilute the TBE with distilled water).
(2)Use a flask that is several times larger than the volume of
buffer.
(3)Boil the mixture until it becomes clear (Be careful when
boiling the agarose solution may become superheated and
may boil violently if it has been heated too long in a
microwave oven).
(4)Wait for the mixture till it cooled down and avoid the vapor
, after that add 1.5l ethidium bromide.
(5)Mix well and wait further time till the mixture becomes
cooler.
(6)Power the mixture into your tray cast with desired combs
and wait till the solidification complete.
(7)Remove the combs.
(8)In a parafilm paper put 3 l loading dye (Bromo Phenol
Blue).
(9)Take 5 l from your PCR product and mix it with the 3 l
loading dye in the paraffin paper and loaded the amount into
the gel wells.
(10)After finish from the loading connect the power with tank
, adjust the desired voltage and time then press on start.
(11)Lastly check the gel in a UV light illuminator or a Gel
Documentation system.
DNA ladder