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ption Factors
The action of transcription factors can either
stimulate or inhibit gene expression.
They must be regulated so that a gene is expressed
only when its product is needed.
The following are a few examples.
Regulation of lac Repressor
The lac operon of E. coli consists of three
genes:- lacZ, lacY, and lacA, encoding -
galactosidase, lactose permease and
thiogalactoside transacetylase, respectively.
Lactose permease is located on the cell membrane,
capable of pumping lactose into the cell.
-galactosidase can convert lactose into glucose
and galactose .
Thiogalactoside transacetylase is responsible for
degrading small molecules.
In the absence of lactose, transcription of
the lac operon is inhibited by the lac repressor .
The lactose can bind to the lac repressor,
preventing it from interacting with its DNA
binding site.
Hence, in a medium containing lactose,
the lac operon is quickly transcribed, producing
the enzymes to generate glucose, which is the
major energy source for E. coli.
Regulation of Repressor (cI)