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Gill Membrane
WATER BLOOD
on the difference in the dissolved
oxygen pressure in the water vs Dissolved O2 Pressure
in Water
that in the blood.
The steeper the pressure gradient Dissolved O2 Pressure
in Blood
the greater the rate of diffusion.
HOW FISH TAKE UP OXYGEN
Temperature, as will be explained, affects dissolved oxygen.
Warm water holds less dissolved oxygen than cold water.
Thus a fish in warm water must pump more water across its gills than in
cold water in order to deliver the same amount of oxygen.
This is necessary even though the pressure gradient between water and
blood is the same.
• PRESSURE (e.g.Altitude)
• TEMPERATURE
• SALINITY
SOLUBILITY OF GASES IN WATER:
PRESSURE:
The amount of gas that can be dissolved in water is proportional to the
pressure of that gas in contact with the water - (Henry’s Law). The higher
the pressure the greater the solubility.
SOLUBILITY OF GASES IN WATER:
PRESSURE:
The amount of gas that can be dissolved in water is proportional to the
pressure of that gas in contact with the water - (Henry’s Law). The higher
the pressure the greater the solubility.
Water becomes saturated with a dissolved gas when the pressure of the
dissolved gas, sometimes referred to as “gas tension”, is equal to the
pressure of that gas in contact with the water.
SOLUBILITY OF GASES IN WATER:
PRESSURE:
The amount of gas that can be dissolved in water is proportional to the
pressure of that gas in contact with the water - (Henry’s Law). The higher
the pressure the greater the solubility.
Water becomes saturated with a dissolved gas when the pressure of the
dissolved gas, sometimes referred to as “gas tension”, is equal to the
pressure of that gas in contact with the water.
Gases moves back and forth across the gas liquid
interface until pressure equilibrium is reached.
If water is supersaturated with a gas, then the gas
will want to escape, like when you pop the cap off
a bottle of pop.
SOLUBILITY OF GASES IN WATER:
PRESSURE vs CONCENTRATION:
The actual concentration of the dissolved gas, e.g. mg/l or ppm
depends on the solubility of the gas:
Gases have different solubility’s:
E.g. Oxygen is 2.3 times more soluble than nitrogen.
Carbon Dioxide is 40 times more soluble than oxygen.
TEMPERATURE:
Cold water can absorb more gas than hot water. The higher the water
temperature the lower the solubility.
SALINITY:
Salinity affects solubility. The higher the salinity the lower the solubility.
For example, oxygen solubility in Sea Water (35 ppt), is approximately
80% that of Fresh Water.
AIR IN CONTACT WITH WATER:
Air is a mixture of gases.
COMPOSITION OF AIR: Nitrogen (N2) 78.08 %
Oxygen (O2) 20.94 %
Argon (Ar) 0.94 %
Carbon Dioxide (CO2) 0.04 %
AIR IN CONTACT WITH WATER:
Air is a mixture of gases.
COMPOSITION OF AIR: Nitrogen (N2) 78.08 %
Oxygen (O2) 20.94 %
Argon (Ar) 0.94 %
Carbon Dioxide (CO2) 0.04 %
In order to determine the solubility of a constituent gas in air, you need to know its Partial Pressure.
The partial pressure of a constituent gas in a mixture of gases is equal to its volume fraction
multiplied by the total pressure of all the gases, which in the case of air, is the Barometric Pressure.
Hence: Barometric Pressure, BP = PN2 + PO2 + PAr + PCO2
So, partial pressure of oxygen, PO2 = 20.94/100 x BP
NOTE: Water Vapour Pressure has been omitted from above for simplicity.
It should be included in actual calculations
AIR IN CONTACT WITH WATER:
The table below shows the respective Partial Pressures and Solubility's of
the constituent gases of Air in Fresh Water at Sea Level. BP = 760 mmHg
(101.3 kPa)
Gill Membrane
WATER BLOOD
are dictated by dissolved O2 pressure, Po2
to maintain the necessary pressure gradient. PO2 Sat. 157 mmHg
72 mmHg
concentration is much lower than in colder PO2 70% 110 mmHg
water with the same Po2, it is the mg/l 25 mmHg
value that determines the requirements PO2 Blood
85 mmHg
for respiration.
DISSOLVED OXYGEN CRITERIA
FOR A HEALTHY FISH POPULATION
From statistical data collected, three levels of protection were derived:
Level A This represents more or less ideal
conditions and assures a high Incipient DO Theshold Levels based on either
degree of safety for fish stocks. PO2 in mmHg or DO Concentration in mg/l
Protection PO2 DO Conc
Level B This is the oxygen value where the GROUP
Level mmHg mg/l
average member of a fish population
starts to exhibit symptoms of distress. Fresh water salmonid A 120 7.75
Some degree of risk exists if the O2 population (incl. B 90 6.00
minimum is prolonged beyond a steelhead) C 60 4.25
few hours. Mixed fresh water fish A 110 7.25
Level C At this level a large portion of a fish population, incl. B 85 5.25
salmonids
population will be affected and if C 60 3.25
prolonged beyond a few hours, the Mixed fresh water fish A 95 5.50
effects can be serious. population with no B 75 4.00
salmonids C 55 2.50
DISSOLVED OXYGEN CRITERIA
FOR A HEALTHY FISH POPULATION
By way of example, we will determine which of the two, mmHg or mg/l, will dictate dissolved
oxygen criteria:
Consider Protection Level A, mixed fresh water Incipient DO Theshold Levels based on either
fish population, BP = 760 mmHg (sea level) PO2 in mmHg or DO Concentration in mg/l
Protection PO2 DO Conc
Fresh Water at 10ºC: PO2 = 157 mmHg GROUP
Level mmHg mg/l
DO Conc = 11.3 mg/l A 120 7.75
Fresh water salmonid
mmHg as % sat. = 110/157 = 70% population (incl. B 90 6.00
mg/l as % sat = 7.25/11.3 = 64% steelhead) C 60 4.25
Therefore PO2 determines DO level. Mixed fresh water fish A 110 7.25
population, incl. B 85 5.25
Fresh Water at 20ºC: PO2 = 157 mmHg
salmonids C 60 3.25
DO Conc = 9.1 mg/l
mmHg as % sat. = 110/157 = 70% Mixed fresh water fish A 95 5.50
mg/l as % sat = 7.25/9.1 = 80% population with no B 75 4.00
salmonids C 55 2.50
Therefore DO Conc. determines DO level.
DISSOLVED OXYGEN CRITERIA FOR A HEALTHY FISH POPULATION
Dissolved Oygen Criteria derived from Incipient DO Theshold Levels
based on PO2 in mmHg and DO Concentration in mg/l
Protection PO2 DO Conc % Sat. values from either mmHg or mg/l, which ever the greater
GROUP
Level mmHg mg/l 0 ºC 5 ºC 10 ºC 15 ºC 20 ºC 25 ºC
Fresh water salmonid A 120 7.75 83.9 84.1 84.4 84.9 94.3 104.8
In these two tables, Dissolved population (incl. B 90 6.00 62.9 63.1 63.3 65.7 73.0 81.1
steelhead)
Oxygen criteria are shown as C 60 4.25 41.9 42.0 42.2 46.6 51.7 57.4
Mixed fresh water fish A 110 7.25 76.9 77.1 77.4 79.4 88.2 98.0
both % Sat (top table) and population, incl. B 85 5.25 59.4 59.6 59.8 60.1 63.9 71.0
salmonids
mg/l (bottom table). C 60 3.25 41.9 42.0 42.2 42.4 42.7 43.9
Mixed fresh water fish A 95 5.50 66.4 66.6 66.8 67.2 67.7 74.3
population with no B 75 4.00 52.4 52.6 52.8 53.0 53.4 54.1
salmonids
NOTE: As BP decreases with C 55 2.50 38.4 38.5 38.7 38.9 39.2 39.5
increasing altitude, PO2 for air Altitude, metres 854 BP, mmHg 688
saturated water gets smaller Dissolved Oygen Criteria derived from Incipient DO Theshold Levels
resulting in higher % Sat. values, based on PO2 in mmHg and DO Concentration in mg/l
Protection PO2 DO Conc mg/l values from either mmHg or mg/l, which ever the greater
but mg/l values remain the same GROUP
Level mmHg mg/l 0 ºC 5 ºC 10 ºC 15 ºC 20 ºC 25 ºC
(higher % Sat. x lower mg/l will Fresh water salmonid A 120 7.75 11.08 9.74 8.63 7.75 7.75 7.75
Yield constant mg/l values) population (incl. B 90 6.00 8.31 7.30 6.47 6.00 6.00 6.00
steelhead) C 60 4.25 5.54 4.87 4.31 4.25 4.25 4.25
Mixed fresh water fish A 110 7.25 10.15 8.92 7.91 7.25 7.25 7.25
population, incl. B 85 5.25 7.85 6.90 6.11 5.49 5.25 5.25
salmonids C 60 3.25 5.54 4.87 4.31 3.87 3.51 3.25
Mixed fresh water fish A 95 5.50 8.77 7.71 6.83 6.13 5.56 5.50
population with no B 75 4.00 6.92 6.08 5.39 4.84 4.39 4.00
salmonids C 55 2.50 5.08 4.46 3.95 3.55 3.22 2.93
LAKES AND PONDS
OXYGENATION OF LAKES AND PONDS:
From Natural Aeration: Oxygen from the air gets absorbed at the surface of the
water. Turbulence and wave action increases surface
area, the greater the surface area the more oxygen is
absorbed.
LAKES AND PONDS
OXYGENATION OF LAKES AND PONDS:
From Natural Aeration: Oxygen from the air gets absorbed at the surface of the
water. Turbulence and wave action increases surface
area, the greater the surface area the more oxygen is
absorbed.
From Photosynthesis of: Most oxygen comes from photosynthesis of aquatic plants,
Aquatic Plants algae and phytoplankton. This only happens during daylight
hours, at night aquatic plants consume oxygen. There is a
diurnal fluctuation in oxygen levels: Rising during the day
when O2 from photosynthesis exceeds O2 demand of
aquatic life, then dropping during the night. On bright sunny
days water can become super saturated with oxygen.
Conversely on dark cloudy days oxygen levels can drop
dangerously low at night.
LAKES AND PONDS
CAUSES OF OXYGEN DEPLETION:
Decomposition of: While healthy algae blooms and aquatic plants will produce enough
Organic Matter O2 to support aquatic life over 24 hour period, dying populations may
consume more O2 than they produce. This plus the decay of other
organic matter can lead to dangerously low oxygen levels.
LAKES AND PONDS
CAUSES OF OXYGEN DEPLETION:
Decomposition of: While healthy algae blooms and aquatic plants will produce enough
Organic Matter O2 to support aquatic life over 24 hour period, dying populations may
consume more O2 than they produce. This plus the decay of other
organic matter can lead to dangerously low oxygen levels.
Eutrophication: Discharge of phosphates and other nutrients from fertilizers,
livestock and sewage induces explosive growth of algae and plants,
the decaying of which depletes dissolved oxygen.
LAKES AND PONDS
CAUSES OF OXYGEN DEPLETION:
Decomposition of: While healthy algae blooms and aquatic plants will produce enough
Organic Matter O2 to support aquatic life over 24 hour period, dying populations may
consume more O2 than they produce. This plus the decay of other
organic matter can lead to dangerously low oxygen levels.
Eutrophication: Discharge of phosphates and other nutrients from fertilizers,
livestock and sewage induces explosive growth of algae and plants,
the decaying of which depletes dissolved oxygen.
Ice & Snow cover: Prolonged ice and snow cover can be a problem. Not only does the
water not have access to air, snow covered ice prevents adequate
sunlight penetration for photosynthesis. This together with decaying
organic matter may lead to Winter Kill. A preventive measure is to
partially clear snow in long strips to allow sunlight to penetrate, or
use an aerator(s) to keep an open area of water.
LAKES AND PONDS
CAUSES OF OXYGEN DEPLETION:
25ºC
Lake Stratification and Turnover:
Epilimnion
Epilimnion: Upper warm layer in contact with the air
higher in dissolved oxygen. Thermocline
Seasonal lake turnover results in the oxygen deficient bottom layer coming to the surface
and mixing with the surface water, sometimes resulting in the entire lake or pond becoming
oxygen deficient.