HISTOLOGY OF SPLEEN

BY Click to edit Master subtitle style

DR. MUDASSAR ALI ROOMI (MBBS,

M. PHIL)

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SPLEEN

Largest lymph organ It lies in the left hypochondrial region it is covered all over by peritoneum except at Hilum Mesothelium (simple squamous epithelium) Lines the outer surface of peritoneum. capsule is dense irregular connective tissue which sends in Trebeculae into 4/26/12 the pulp of spleen

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Stroma of spleen
1. 2. 3.
.

C.T. Capsule Trabeculae Reticulum:

it is Framework of spleen Amorphous material with:

fine collagen fibers Reticular Cells. 33

Spaces in reticulum are 4/26/12 filled in by Splenic Pulp

Parenchyma of spleen

Red pulp white pulp

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White Pulp of spleen

(a)

Includes Lymphoid tissue (both diffuse and nodular) Periarterial lymphatic sheats (PALS) surrounding splenic arteriole. replacing their Tunica adventitia. It contains T-lymphocytes.

(b)

(c)
()

Splenic nodule (Malpighian corpuscle) B Lymphocytes (Bone marrow derived). Visible to naked eye as white dots. 0.25 1 mm.. Germinal centre may be present . mantle arteriole entering splenic nodule from periarterial lymph sheath eccentric in position (Central Artery) with 4/26/12 T. Media. definite

()

()

()

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Red Pulp
I.

. .

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SPLENIC Sinusoids No lymphatics Cells modified reticular cells Long axis of cells lies parallel to the long axis of sinusoids

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HISTOLOGY OF SPLLEN

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HOW TO DRAW IT?

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Differences between splenic sinuses and ordinary capillaries

1.

Splenic sinusoids have irregular lumen and are highly distensible Have specialized endothelial cells (littoral cells) in their wall which are rod shaped and run longitudinally in the vessel wall Large gaps between the endothelial cells Incomplete basal lamina
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2.

3.

4.

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Functions of spleen
1.

Filtration of blood: bacteria are removed. Production of lymphocytes: occurs in the white pulp. Destruction of old and worn out erythrocytes: spleen is the graveyard of RBCs. This function is carried out by macrophages in the wall of sinusoids. Storage of blood:
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2.

3.

4.

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Identification points of spleen

Peritoneal covering (serosa) Red and white pulp No differentiation into cortex and medulla Splenic nodules with central arteriole

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SPLLEN Vs Lymph Node

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SPLENIC CIRCULATION

Splenic artery : its 4-5 rami enter the Hilum. Along trabeculae Finest arterioles pass out of Trebaculae Adventitia 4/26/12 replaced by
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Blood Circulation in Spleen

Splenic Artery 5 or 4 branches Enter trabelulae Small arteriole branches (Periart Lymphatic sheath surrounds it) Central Arterioles in Splenic Nodule Eccentric position Branches to Splenic Nodule Arterioles lose thin Lymph Sheath divide in Red Pulp 4/26/12

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Theories of blood flow in spleen

1.

Closed Theory
It states that the terminal arterial capillaries open directly into the venous sinuses.

2.
.

Open Theory
that
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4/26/12 It states

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